SSGCID: Target Page

PsaeA.17928.a

Glycine--tRNA ligase beta subunit (EC 6.1.1.14) (Glycyl-tRNA synthetase beta subunit) (GlyRS)

CENTER ID:	PsaeA.17928.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17928.a.B1.GE38378	full length		1	684
PsaeA.17928.a.B2.GE40331	catalytic central domain		1	570

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17928.a.B2.PS38534	catalytic central domain		1	570
PsaeA.17928.a.B1.PW37596	full length		1	684

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.8
PGAT:	pseudomonas:PA0008
RefSeq:	NP_064728.1
UniProt:	Q9I7B8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAKDFLVEL GTEELPPKAL NSLGEAFLSG IEKGLKAAGL SYAAARFYAA PRRLAVLVEQ LAVQQPDRTV NLDGPPLQAA FDASGNPTQA ALGFAKKCGV DLQQIDKSGP KLRFIQTIAG QPAAGLLPGI VEASLNELPI PKRMRWAARR EEFVRPTQWL VMLFGDDVVE CEILAQKAGR ESRGHRFHNP DNVRISSPAA YLEDLRGAHV LADFAERREL IAKRVAELAA EQQGSAIVPP SLLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDANG KLLPRFITVA NVESKAPENI VSGNEKVVRP RLTDAEFFFK QDKKQPLESF NERLRNVVFQ AQLGTVFEKA QRVSGLAAYI AERIGGNAQN ASRAGILSKC DLATEMVGEF PEMQGIAGYY YATHGGEAED VALALNEQYM PRGAGAELPS TLTGAAVAVA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL GVLRILIEKQ LDLDLVAAVN AAVEQYGDKV KAAGLAEQVL DFVFDRLRAR YEDEGVDVAV YQSVRALKPS SPLDFDQRVQ AVQAFRQLPE AEALAAANKR VSNILAKSED EVPPNVDASL LVEAAEKALG SAVANAESEV APLAAARDYR AALARLAALR EPVDTFFADV MVNVDDAAVR ANRYALLAKL RGSFLGVADI SLLG

NT Sequence

atgagcgcca aggatttcct ggtcgaactg ggcaccgaag agctgccgcc aaaagccctc aacagcctcg gcgaagcctt cctcagcggt atcgagaagg gcctcaaggc tgccggtctg agctatgccg ctgcgcgttt ctatgcggcg ccgcgccgtc tcgccgtgct ggtcgagcaa ctcgccgtgc agcagcccga ccgtaccgtc aacctagacg gtccgccgct acaggccgcc ttcgacgcca gcggtaaccc gacccaggcc gccctgggct tcgccaagaa gtgcggcgtg gatctgcagc agatcgacaa gagcggtccg aagctgcgct tcatccagac catcgccggc caaccggccg ccggcctgct gccgggcatc gtcgaggcct cgttgaacga actgccgatt cccaagcgga tgcgctgggc agctcgccgc gaggagttcg tgcgtccgac ccaatggctg gtgatgctgt tcggcgacga tgtggtcgag tgcgagatcc tcgcccagaa agccgggcgc gaatcccgtg gccaccgctt ccacaatccg gacaatgtgc gtatttccag cccggcagcg tatctggaag acctgcgcgg cgctcatgtg ctggccgatt tcgccgagcg ccgcgagctg atcgccaagc gcgtggccga actggccgcc gagcaacagg gcagcgccat cgtgccgccg agcctgctcg acgaagtgac tgcactggtc gagtggccgg tgccgctggt ctgctccttc gaggagcgct tcctcgaggt cccgcaggaa gccctgatca ccaccatgca ggacaaccag aaatacttct gcctgctgga cgccaacggc aagctgctgc ctcgcttcat caccgtcgcc aacgtcgaga gcaaggcgcc ggagaacatc gtcagcggta acgagaaggt ggttcgcccg cgcctcaccg acgccgagtt cttcttcaag caggacaaga agcagccgct cgaaagcttc aatgagcgcc tgcgcaacgt agtgttccag gcccagctcg gcaccgtgtt cgagaaggcc cagcgcgtct ccggcctggc cgcctacatc gccgaacgta taggcggcaa tgcgcagaac gcctcgcgcg ccggcatcct gtccaagtgt gacctggcga ccgagatggt cggcgagttc cccgaaatgc agggcatcgc cggctactac tacgccaccc acggcggcga agcggaagac gtcgccctgg ccctcaacga gcagtacatg ccgcgcggcg ccggcgccga gctaccctcg accctgaccg gcgccgccgt ggcggtggcc gacaagctcg ataccctggt cggtatcttc ggcatcggca tgcttcccac cggcagcaag gacccctacg cgctgcgccg cgctgcgctg ggcgtgctgc gcatcctcat cgagaagcag ctcgacctgg acctggtagc cgcggtcaac gccgccgtcg agcaatacgg cgacaaggtc aaggccgccg gcctggccga gcaggtgctg gacttcgtgt tcgaccgcct gcgcgcgcgc tacgaggacg aaggcgtgga cgtggccgtg taccagtcgg tgcgcgcgct caagccaagc tcgccgctgg acttcgacca gcgcgtacag gccgtccagg ccttccgcca gttgcctgaa gccgaggccc tggccgcggc gaacaaacgg gtgtcgaata ttctcgccaa gtccgaggac gaggttccgc cgaacgtgga tgccagcctg ctggtggaag ccgccgagaa ggccctgggc agcgccgtgg cgaacgccga aagcgaagtc gcgccgctgg cagcggcacg cgactatcgc gccgcgctgg cccgcctggc ggccttgcgc gagccggtgg atacgttctt cgccgatgtg atggtcaatg tcgacgacgc ggcggtgcgc gccaaccgct atgcgctgct ggccaagctg cgcgggtcgt tcctcggggt ggcggacatc tcgctgctcg gctga

Details for PsaeA.17928.a.B1.GE38378

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	75kDa
OBSERVED MW:	78kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	78
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

anncnntagn ntnttttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg agcgccaagg atttcctggt cgaactgggc accgaagagc tgccgccaaa agccctcaac agcctcggcg aagccttcct cagcggtatc gagaagggcc tcaaggctgc cggtctgagc tatgccgctg cgcgtttcta tgcggcgccg cgccgtctcg ccgtgctggt cgagcaactc gccgtgcagc agcccgaccg taccgtcaac ctagacggtc cgccgctaca ggccgccttc gacgccagcg gtaacccgac ccaggccgcc ctgggcttcg ccaagaagtg cggcgtggat ctgcagcaga tcgacaagag cggtccgaag ctgcgcttca tccagaccat cgccggccaa ccggccgccg gcctgctgcc gggcatcgtc gaggcctcgt tgaacgaact gccgattccc aagcggatgc gctgggcagc tcgccgcgag gagttcgtgc gtccgaccca atggctggtg atgctgttcg gcgacgatgt ggtcgagtgc gagatcctcg cccagaaagc cgggcgcgaa tcccgtggcc accgcttcca caatccggac aatgtgcgta tttccagccc ggcagcgtat ctggaagacc tgcgcggcgc tcatgtgctg gccgatttcg ccgagcgccg cgagctgatc gccaagcgcg tggccgaact ggccgccgag caacagggca gcgccatcgt gccgccgagc ctgctcgacg aagtgactgc actggtcgag tggccggtgc cgctggtctg ctccttcgag gagcgcttcc tcgaggtccc gcaggaagcc ctgatcacca ccatgcngac aaccagaaat acttctgcct gctggacgcc aacggcaagc tgctgcctcg cttcatcacc gtcgcnaacg tcgagagcaa gnngccnnan aatcgtcagc ggtaacgaga nnggnngccg ngctcacgac ncganttcnt ntcaagcngn nagagcagcn ctcgaaagct tcanggannn cngncnnacg tnnnnnnnnn nnnnngnncn nntcnanann cagcnngtcn cnntnnnncc tanncgccnn nnnnnnnnnn ngcnnnnnnn gcnnnngnnt tcnnntncca agngtgngaa c

Expected Protein Sequence

MAHHHHHHMS AKDFLVELGT EELPPKALNS LGEAFLSGIE KGLKAAGLSY AAARFYAAPR RLAVLVEQLA VQQPDRTVNL DGPPLQAAFD ASGNPTQAAL GFAKKCGVDL QQIDKSGPKL RFIQTIAGQP AAGLLPGIVE ASLNELPIPK RMRWAARREE FVRPTQWLVM LFGDDVVECE ILAQKAGRES RGHRFHNPDN VRISSPAAYL EDLRGAHVLA DFAERRELIA KRVAELAAEQ QGSAIVPPSL LDEVTALVEW PVPLVCSFEE RFLEVPQEAL ITTMQDNQKY FCLLDANGKL LPRFITVANV ESKAPENIVS GNEKVVRPRL TDAEFFFKQD KKQPLESFNE RLRNVVFQAQ LGTVFEKAQR VSGLAAYIAE RIGGNAQNAS RAGILSKCDL ATEMVGEFPE MQGIAGYYYA THGGEAEDVA LALNEQYMPR GAGAELPSTL TGAAVAVADK LDTLVGIFGI GMLPTGSKDP YALRRAALGV LRILIEKQLD LDLVAAVNAA VEQYGDKVKA AGLAEQVLDF VFDRLRARYE DEGVDVAVYQ SVRALKPSSP LDFDQRVQAV QAFRQLPEAE ALAAANKRVS NILAKSEDEV PPNVDASLLV EAAEKALGSA VANAESEVAP LAAARDYRAA LARLAALREP VDTFFADVMV NVDDAAVRAN RYALLAKLRG SFLGVADISL LG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ccaaggattt cctggtcgaa ctgggcaccg aagagctgcc gccaaaagcc ctcaacagcc tcggcgaagc cttcctcagc ggtatcgaga agggcctcaa ggctgccggt ctgagctatg ccgctgcgcg tttctatgcg gcgccgcgcc gtctcgccgt gctggtcgag caactcgccg tgcagcagcc cgaccgtacc gtcaacctag acggtccgcc gctacaggcc gccttcgacg ccagcggtaa cccgacccag gccgccctgg gcttcgccaa gaagtgcggc gtggatctgc agcagatcga caagagcggt ccgaagctgc gcttcatcca gaccatcgcc ggccaaccgg ccgccggcct gctgccgggc atcgtcgagg cctcgttgaa cgaactgccg attcccaagc ggatgcgctg ggcagctcgc cgcgaggagt tcgtgcgtcc gacccaatgg ctggtgatgc tgttcggcga cgatgtggtc gagtgcgaga tcctcgccca gaaagccggg cgcgaatccc gtggccaccg cttccacaat ccggacaatg tgcgtatttc cagcccggca gcgtatctgg aagacctgcg cggcgctcat gtgctggccg atttcgccga gcgccgcgag ctgatcgcca agcgcgtggc cgaactggcc gccgagcaac agggcagcgc catcgtgccg ccgagcctgc tcgacgaagt gactgcactg gtcgagtggc cggtgccgct ggtctgctcc ttcgaggagc gcttcctcga ggtcccgcag gaagccctga tcaccaccat gcaggacaac cagaaatact tctgcctgct ggacgccaac ggcaagctgc tgcctcgctt catcaccgtc gccaacgtcg agagcaaggc gccggagaac atcgtcagcg gtaacgagaa ggtggttcgc ccgcgcctca ccgacgccga gttcttcttc aagcaggaca agaagcagcc gctcgaaagc ttcaatgagc gcctgcgcaa cgtagtgttc caggcccagc tcggcaccgt gttcgagaag gcccagcgcg tctccggcct ggccgcctac atcgccgaac gtataggcgg caatgcgcag aacgcctcgc gcgccggcat cctgtccaag tgtgacctgg cgaccgagat ggtcggcgag ttccccgaaa tgcagggcat cgccggctac tactacgcca cccacggcgg cgaagcggaa gacgtcgccc tggccctcaa cgagcagtac atgccgcgcg gcgccggcgc cgagctaccc tcgaccctga ccggcgccgc cgtggcggtg gccgacaagc tcgataccct ggtcggtatc ttcggcatcg gcatgcttcc caccggcagc aaggacccct acgcgctgcg ccgcgctgcg ctgggcgtgc tgcgcatcct catcgagaag cagctcgacc tggacctggt agccgcggtc aacgccgccg tcgagcaata cggcgacaag gtcaaggccg ccggcctggc cgagcaggtg ctggacttcg tgttcgaccg cctgcgcgcg cgctacgagg acgaaggcgt ggacgtggcc gtgtaccagt cggtgcgcgc gctcaagcca agctcgccgc tggacttcga ccagcgcgta caggccgtcc aggccttccg ccagttgcct gaagccgagg ccctggccgc ggcgaacaaa cgggtgtcga atattctcgc caagtccgag gacgaggttc cgccgaacgt ggatgccagc ctgctggtgg aagccgccga gaaggccctg ggcagcgccg tggcgaacgc cgaaagcgaa gtcgcgccgc tggcagcggc acgcgactat cgcgccgcgc tggcccgcct ggcggccttg cgcgagccgg tggatacgtt cttcgccgat gtgatggtca atgtcgacga cgcggcggtg cgcgccaacc gctatgcgct gctggccaag ctgcgcgggt cgttcctcgg ggtggcggac atctcgctgc tcggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for PsaeA.17928.a.B2.GE40331

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	74
PERCENT COVERAGE:	60

Validated AA Sequence

Validated NT Sequence

cnnnnnnnnn ngcaccgact ggnannngnn nangtccann ncttcgncnn ngnngnnnnc gcgcagnnnn nnnnnnnnnn nnnnnnnnnn cnnnnnnnnc cannnnnnnn gnnnttgacc ttnnnnncnn nttgctcnnn nnnnnnnnng accgnnncta ccannnnnng gnnnngctgc ttctnnnnnn nnatgnnnac cnnncccnnn nnnnnncgnn nnnnnnnnnn nnnntccttg ctgccggtgg nnnnnntgcc gatgcnnnnn ntncnnncca ggntatcgag cntnnnngcc acnnncacgg cggcgccggt nnnnntcgag ggtagctcgg cgccggcgcc nnnnngcatg tactgctcgt tgannncnnn ngngnnnnnn tccgcttcgn nnccnnnnnt ggcgtnnnnn nnnncngcga tgccctgcat ttnnnggaac tcgcnnncca tctcgnnngc caggtcacac ttngacagga tgccggnnng cgaggcgttc tgcgnattgc nnnctatang ttcggcgatg tnnnnngnca ggnnngannn nnnctgggcc ttctcgaaca cgntnnnnnn ntgngcctgg aacactacgt tgcnnnnnnn ctcattnnnn ntttnnngcg gctgcttctt gtcctgcnnn nnnnnnnact cggnnnnnnt gaggcgcnnn nnnnnnnnnn tnnngttnnn nnnnnnnntg ttctcngnnn ccttgctnnn nnnnnnnnnn annnnnntgn nnnnnggnnn nnnnnnnnnn nnnnnntnnn nnnnnnngan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggnnn nnnnnnnnnn nnnnnnnnaa ggnnnannnc agcggcaccg nnnactcgac cagtgcagtc nnnnnntcga gcagnctcgg cggcacgatg gcgctgccct gntgctcggc ggccagttcg gccacgcgct tggcgatcag ctcgcggcgc tcggcgaaat cggccagcac atgagcgccg cgcaggtctt ccagatacgc tgccgggctg gaaatacgca cattgtccgg attgtggaag cggtggccac gggattcgcg cccggctttc tgggcgagga tctcgcactc gaccacatcg tcgccgaaca gcatcaccag ccattgggtc ggacgcacga actcctcgcg gcgagctgcc cagcgcatcc gcttgggaat cggcagttcg ttcaacgagg cctcgacgat gcccggcagc aggccggcgg ccggttggcc ggcgatggtc tggatgaagc gcagcttcgg accgctcttg tcgatctgct gcagatccac gccgcacttc ttggcgaagc ccagggcggc ctgggtcggg ttaccgctgg cgtcgaaggc ggcctgtagc ggcggaccgt ctaggttgac ggtacggtcg ggctgctgca cggcgagttg ctcgaccagc acggcgagac ggcgcggcgc cgcatagaaa cgcgcagcgg catagctcag accggcagcc ttgaggccct tctcgatacc gctgaggaag gcttcgccga ggctgttgag ggcttttggc ggcagctctt cggtgcccag ttcgaccagg aaatccttgg cgctcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ccaaggattt cctggtcgaa ctgggcaccg aagagctgcc gccaaaagcc ctcaacagcc tcggcgaagc cttcctcagc ggtatcgaga agggcctcaa ggctgccggt ctgagctatg ccgctgcgcg tttctatgcg gcgccgcgcc gtctcgccgt gctggtcgag caactcgccg tgcagcagcc cgaccgtacc gtcaacctag acggtccgcc gctacaggcc gccttcgacg ccagcggtaa cccgacccag gccgccctgg gcttcgccaa gaagtgcggc gtggatctgc agcagatcga caagagcggt ccgaagctgc gcttcatcca gaccatcgcc ggccaaccgg ccgccggcct gctgccgggc atcgtcgagg cctcgttgaa cgaactgccg attcccaagc ggatgcgctg ggcagctcgc cgcgaggagt tcgtgcgtcc gacccaatgg ctggtgatgc tgttcggcga cgatgtggtc gagtgcgaga tcctcgccca gaaagccggg cgcgaatccc gtggccaccg cttccacaat ccggacaatg tgcgtatttc cagcccggca gcgtatctgg aagacctgcg cggcgctcat gtgctggccg atttcgccga gcgccgcgag ctgatcgcca agcgcgtggc cgaactggcc gccgagcaac agggcagcgc catcgtgccg ccgagcctgc tcgacgaagt gactgcactg gtcgagtggc cggtgccgct ggtctgctcc ttcgaggagc gcttcctcga ggtcccgcag gaagccctga tcaccaccat gcaggacaac cagaaatact tctgcctgct ggacgccaac ggcaagctgc tgcctcgctt catcaccgtc gccaacgtcg agagcaaggc gccggagaac atcgtcagcg gtaacgagaa ggtggttcgc ccgcgcctca ccgacgccga gttcttcttc aagcaggaca agaagcagcc gctcgaaagc ttcaatgagc gcctgcgcaa cgtagtgttc caggcccagc tcggcaccgt gttcgagaag gcccagcgcg tctccggcct ggccgcctac atcgccgaac gtataggcgg caatgcgcag aacgcctcgc gcgccggcat cctgtccaag tgtgacctgg cgaccgagat ggtcggcgag ttccccgaaa tgcagggcat cgccggctac tactacgcca cccacggcgg cgaagcggaa gacgtcgccc tggccctcaa cgagcagtac atgccgcgcg gcgccggcgc cgagctaccc tcgaccctga ccggcgccgc cgtggcggtg gccgacaagc tcgataccct ggtcggtatc ttcggcatcg gcatgcttcc caccggcagc aaggacccct acgcgctgcg ccgcgctgcg ctgggcgtgc tgcgcatcct catcgagaag cagctcgacc tggacctggt agccgcggtc aacgccgccg tcgagcaata cggcgacaag gtcaaggccg ccggcctggc cgagcaggtg ctggacttcg tgttcgaccg cctgcgcgcg cgctacgagg acgaaggcgt ggacgtggcc gtgtaccagt cggtgcgcgc gctcaagcca agctcgccgc tggacttcga ccagcgcgta caggccgtcc aggccttccg ccagttgcct gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for PsaeA.17928.a.B2.PS38534

PURIFICATION DATe:	11/20/2018
CONCENTRATION:	4.08mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	74
PERCENT COVERAGE:	60

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CCAAGGATTT CCTGGTCGAA CTGGGCACCG AAGAGCTGCC GCCAAAAGCC CTCAACAGCC TCGGCGAAGC CTTCCTCAGC GGTATCGAGA AGGGCCTCAA GGCTGCCGGT CTGAGCTATG CCGCTGCGCG TTTCTATGCG GCGCCGCGCC GTCTCGCCGT GCTGGTCGAG CAACTCGCCG TGCAGCAGCC CGACCGTACC GTCAACCTAG ACGGTCCGCC GCTACAGGCC GCCTTCGACG CCAGCGGTAA CCCGACCCAG GCCGCCCTGG GCTTCGCCAA GAAGTGCGGC GTGGATCTGC AGCAGATCGA CAAGAGCGGT CCGAAGCTGC GCTTCATCCA GACCATCGCC GGCCAACCGG CCGCCGGCCT GCTGCCGGGC ATCGTCGAGG CCTCGTTGAA CGAACTGCCG ATTCCCAAGC GGATGCGCTG GGCAGCTCGC CGCGAGGAGT TCGTGCGTCC GACCCAATGG CTGGTGATGC TGTTCGGCGA CGATGTGGTC GAGTGCGAGA TCCTCGCCCA GAAAGCCGGG CGCGAATCCC GTGGCCACCG CTTCCACAAT CCGGACAATG TGCGTATTTC CAGCCCGGCA GCGTATCTGG AAGACCTGCG CGGCGCTCAT GTGCTGGCCG ATTTCGCCGA GCGCCGCGAG CTGATCGCCA AGCGCGTGGC CGAACTGGCC GCCGAGCAAC AGGGCAGCGC CATCGTGCCG CCGAGCCTGC TCGACGAAGT GACTGCACTG GTCGAGTGGC CGGTGCCGCT GGTCTGCTCC TTCGAGGAGC GCTTCCTCGA GGTCCCGCAG GAAGCCCTGA TCACCACCAT GCAGGACAAC CAGAAATACT TCTGCCTGCT GGACGCCAAC GGCAAGCTGC TGCCTCGCTT CATCACCGTC GCCAACGTCG AGAGCAAGGC GCCGGAGAAC ATCGTCAGCG GTAACGAGAA GGTGGTTCGC CCGCGCCTCA CCGACGCCGA GTTCTTCTTC AAGCAGGACA AGAAGCAGCC GCTCGAAAGC TTCAATGAGC GCCTGCGCAA CGTAGTGTTC CAGGCCCAGC TCGGCACCGT GTTCGAGAAG GCCCAGCGCG TCTCCGGCCT GGCCGCCTAC ATCGCCGAAC GTATAGGCGG CAATGCGCAG AACGCCTCGC GCGCCGGCAT CCTGTCCAAG TGTGACCTGG CGACCGAGAT GGTCGGCGAG TTCCCCGAAA TGCAGGGCAT CGCCGGCTAC TACTACGCCA CCCACGGCGG CGAAGCGGAA GACGTCGCCC TGGCCCTCAA CGAGCAGTAC ATGCCGCGCG GCGCCGGCGC CGAGCTACCC TCGACCCTGA CCGGCGCCGC CGTGGCGGTG GCCGACAAGC TCGATACCCT GGTCGGTATC TTCGGCATCG GCATGCTTCC CACCGGCAGC AAGGACCCCT ACGCGCTGCG CCGCGCTGCG CTGGGCGTGC TGCGCATCCT CATCGAGAAG CAGCTCGACC TGGACCTGGT AGCCGCGGTC AACGCCGCCG TCGAGCAATA CGGCGACAAG GTCAAGGCCG CCGGCCTGGC CGAGCAGGTG CTGGACTTCG TGTTCGACCG CCTGCGCGCG CGCTACGAGG ACGAAGGCGT GGACGTGGCC GTGTACCAGT CGGTGCGCGC GCTCAAGCCA AGCTCGCCGC TGGACTTCGA CCAGCGCGTA CAGGCCGTCC AGGCCTTCCG CCAGTTGCCT GAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT

Details for PsaeA.17928.a.B1.PW37596

PURIFICATION DATe:	11/11/2014
CONCENTRATION:	28mg/ml
OBSERVED MW:	76kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	78
PERCENT COVERAGE:	55

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CCAAGGATTT CCTGGTCGAA CTGGGCACCG AAGAGCTGCC GCCAAAAGCC CTCAACAGCC TCGGCGAAGC CTTCCTCAGC GGTATCGAGA AGGGCCTCAA GGCTGCCGGT CTGAGCTATG CCGCTGCGCG TTTCTATGCG GCGCCGCGCC GTCTCGCCGT GCTGGTCGAG CAACTCGCCG TGCAGCAGCC CGACCGTACC GTCAACCTAG ACGGTCCGCC GCTACAGGCC GCCTTCGACG CCAGCGGTAA CCCGACCCAG GCCGCCCTGG GCTTCGCCAA GAAGTGCGGC GTGGATCTGC AGCAGATCGA CAAGAGCGGT CCGAAGCTGC GCTTCATCCA GACCATCGCC GGCCAACCGG CCGCCGGCCT GCTGCCGGGC ATCGTCGAGG CCTCGTTGAA CGAACTGCCG ATTCCCAAGC GGATGCGCTG GGCAGCTCGC CGCGAGGAGT TCGTGCGTCC GACCCAATGG CTGGTGATGC TGTTCGGCGA CGATGTGGTC GAGTGCGAGA TCCTCGCCCA GAAAGCCGGG CGCGAATCCC GTGGCCACCG CTTCCACAAT CCGGACAATG TGCGTATTTC CAGCCCGGCA GCGTATCTGG AAGACCTGCG CGGCGCTCAT GTGCTGGCCG ATTTCGCCGA GCGCCGCGAG CTGATCGCCA AGCGCGTGGC CGAACTGGCC GCCGAGCAAC AGGGCAGCGC CATCGTGCCG CCGAGCCTGC TCGACGAAGT GACTGCACTG GTCGAGTGGC CGGTGCCGCT GGTCTGCTCC TTCGAGGAGC GCTTCCTCGA GGTCCCGCAG GAAGCCCTGA TCACCACCAT GCAGGACAAC CAGAAATACT TCTGCCTGCT GGACGCCAAC GGCAAGCTGC TGCCTCGCTT CATCACCGTC GCCAACGTCG AGAGCAAGGC GCCGGAGAAC ATCGTCAGCG GTAACGAGAA GGTGGTTCGC CCGCGCCTCA CCGACGCCGA GTTCTTCTTC AAGCAGGACA AGAAGCAGCC GCTCGAAAGC TTCAATGAGC GCCTGCGCAA CGTAGTGTTC CAGGCCCAGC TCGGCACCGT GTTCGAGAAG GCCCAGCGCG TCTCCGGCCT GGCCGCCTAC ATCGCCGAAC GTATAGGCGG CAATGCGCAG AACGCCTCGC GCGCCGGCAT CCTGTCCAAG TGTGACCTGG CGACCGAGAT GGTCGGCGAG TTCCCCGAAA TGCAGGGCAT CGCCGGCTAC TACTACGCCA CCCACGGCGG CGAAGCGGAA GACGTCGCCC TGGCCCTCAA CGAGCAGTAC ATGCCGCGCG GCGCCGGCGC CGAGCTACCC TCGACCCTGA CCGGCGCCGC CGTGGCGGTG GCCGACAAGC TCGATACCCT GGTCGGTATC TTCGGCATCG GCATGCTTCC CACCGGCAGC AAGGACCCCT ACGCGCTGCG CCGCGCTGCG CTGGGCGTGC TGCGCATCCT CATCGAGAAG CAGCTCGACC TGGACCTGGT AGCCGCGGTC AACGCCGCCG TCGAGCAATA CGGCGACAAG GTCAAGGCCG CCGGCCTGGC CGAGCAGGTG CTGGACTTCG TGTTCGACCG CCTGCGCGCG CGCTACGAGG ACGAAGGCGT GGACGTGGCC GTGTACCAGT CGGTGCGCGC GCTCAAGCCA AGCTCGCCGC TGGACTTCGA CCAGCGCGTA CAGGCCGTCC AGGCCTTCCG CCAGTTGCCT GAAGCCGAGG CCCTGGCCGC GGCGAACAAA CGGGTGTCGA ATATTCTCGC CAAGTCCGAG GACGAGGTTC CGCCGAACGT GGATGCCAGC CTGCTGGTGG AAGCCGCCGA GAAGGCCCTG GGCAGCGCCG TGGCGAACGC CGAAAGCGAA GTCGCGCCGC TGGCAGCGGC ACGCGACTAT CGCGCCGCGC TGGCCCGCCT GGCGGCCTTG CGCGAGCCGG TGGATACGTT CTTCGCCGAT GTGATGGTCA ATGTCGACGA CGCGGCGGTG CGCGCCAACC GCTATGCGCT GCTGGCCAAG CTGCGCGGGT CGTTCCTCGG GGTGGCGGAC ATCTCGCTGC TCGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT