SSGCID: Target Page

PsaeA.17926.a

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (D-fructose-6-phosphate amidotransferase) (GFAT) (Glucosamine-6-phosphate synthase) (Hexosephosphate aminotransferase) (L-glutamine--D-fructose-6-phosphate amidotransferase)

CENTER ID:	PsaeA.17926.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17926.a.B1.GE38376	full length		1	611

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5815
PGAT:	pseudomonas:PA5549
RefSeq:	NP_254236.1
UniProt:	Q9HT25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCGIVGAIAE RNITPILIEG LKRLEYRGYD SAGVAVFDNE GRLQRCRRVG KVASLEEGLA GTPLLGRLGI AHTRWATHGA PTEGNAHPHF SSDEVAVVHN GIIENHEPLR ERLKGLGYVF TSQTDTEVIV HLLHHKLQSI GDLTLALKDA VKELHGAYGL AVISAAQPDR IVAARSGSPL VIGLGLGENF LASDQLALRQ VTDRFIYLEE GDIAEIRRDS VRLWDVQGND VQRETVQYHE GAEAADKGEY RHFMLKEIHE QPSVVQRTLE GRLGQNQVLV ESFGPQAAEL FAKVRNVQIV ACGTSYHAGM VARYWLESLT GIPCQVEVAS EFRYRKVAVQ PDCLFVTISQ SGETADTLAA LRNAKELGFL SSVAICNVAT SSLVRESDLT LLTQAGPEIG VASTKAFTTQ LVALLLLTLG IGQVQKRLAD GVEAELVDEL RRLPTRLGEA LAMNRTVEKV SELFAEKHHT LFLGRGAQFP VALEGALKLK EISYIHAEAY PAGELKHGPL ALVDSDMPVV TVAPNNELVE KLKSNLQEVR ARGGELVVFA DEGAGIEAGE GTHVVGMPHI GDVLSPILYT IPLQLLSYHV AVLKGTDVDQ PRNLAKSVTV E

NT Sequence

atgtgtggaa tcgtaggcgc gatcgccgag cgcaacatca cccccatcct tatcgaaggc ctcaagcgcc tcgagtatcg cggctacgac agcgccggcg tggcggtctt cgataacgaa ggcaggctgc agcgctgccg ccgggtcggc aaggtcgcca gcctggagga aggcctggcc ggtacgccgc tgctcggccg cctgggcatc gcccataccc gctgggcgac ccacggcgcg ccaaccgaag gcaacgccca cccgcatttc tccagcgacg aagtggcggt ggtgcacaac ggcatcatcg agaaccacga acctctccgt gagcggctca agggcctggg ctacgtgttc acctcgcaga ccgacaccga ggtcatcgtc cacctgctgc accacaagct gcagtccatc ggcgacctga ccctggcgct gaaggacgcg gtcaaggaat tgcacggggc ctatggcctg gcagtgatca gcgcggcgca gccggaccgc atcgtcgccg ctcgcagcgg cagcccgctg gtgatcggcc tcgggcttgg cgagaacttc ctcgcttccg accaactggc gctgcgccag gtgaccgacc gcttcatcta cctggaagaa ggcgatatcg ccgagatccg tcgcgacagc gtgcgcctgt gggatgtcca gggcaacgac gtgcagcgcg agaccgtgca gtaccacgaa ggcgccgaag ccgccgacaa gggcgagtac cggcacttca tgctcaagga aatccacgag cagcccagcg tcgtgcagcg caccctggaa ggccgcctcg ggcagaacca ggtactggtg gaaagcttcg gtccgcaggc ggcggagctg ttcgccaagg tccgcaacgt gcagatcgtc gcctgcggca ccagttacca cgccggcatg gtcgcccgtt actggctgga aagcctgacc gggattccct gccaggtgga ggtcgccagc gagttccgct accgcaaggt cgcggtgcag ccggattgcc tgttcgtcac catctcccag tccggtgaga ccgccgacac cctggctgcc ctgcgcaacg ccaaggagct gggcttcctc tccagcgtgg cgatctgcaa cgttgccacc agttcgctgg tgcgcgaatc cgacctgacc ctgctgaccc aggccggccc ggaaatcggc gtggcctcga ccaaggcctt caccacccag ttggtcgccc tgctgctgct gaccctgggc atcggccagg tgcagaagcg cctggccgat ggcgtcgaag ccgagctggt ggacgaactg cgtcgcctgc cgacccggct gggcgaggcg ctggcgatga accggacggt ggagaaggtt tccgagctgt tcgccgagaa gcaccacacc ctgttcctcg gccgtggcgc gcagttcccg gtggccctgg aaggcgcgct gaagctcaag gaaatttcct atatccatgc cgaagcctac ccggccggcg aactcaagca tggcccgctg gcgctggtgg acagcgacat gccggtggtt accgtggcgc cgaacaacga gctggtggag aagctcaagt cgaacctgca ggaagtccgc gcccgcggcg gcgaactggt ggtgttcgcc gacgaaggcg cgggcatcga ggctggcgag ggcacccatg tggtgggcat gccgcacatc ggcgacgtgc tctcgccgat cctctacacc atcccgttgc aattgctctc gtaccatgtc gccgtgctca agggcaccga cgtcgaccag ccgcgcaacc tggccaagtc ggtcaccgtc gagtaa

Details for PsaeA.17926.a.B1.GE38376

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	67kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	79
PERCENT COVERAGE:	63

Validated AA Sequence

Validated NT Sequence

antncnntan nnnntttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg tgtggaatcg taggcgcgat cgccgagcgc aacatcaccc ccatccttat cgaaggcctc aagcgcctcg agtatcgcgg ctacgacagc gccggcgtgg cggtcttcga taacgaaggc aggctgcagc gctgccgccg ggtcggcaag gtcgccagcc tggaggaagg cctggccggt acgccgctgc tcggccgcct gggcatcgcc catacccgct gggcgaccca cggcgcgcca accgaaggca acgcccaccc gcatttctcc agcgacgaag tggcggtggt gcacaacggc atcatcgaga accacgaacc tctccgtgag cggctcaagg gcctgggcta cgtgttcacc tcgcagaccg acaccgaggt catcgtccac ctgctgcacc acaagctgca gtccatcggc gacctgaccc tggcgctgaa ggacgcggtc aaggaattgc acggggccta tggcctggca gtgatcagcg cggcgcagcc ggaccgcatc gtcgccgctc gcagcggcag cccgctggtg atcggcctcg ggcttggcga gaacttcctc gcttccgacc aactggcgct gcgccaggtg accgaccgct tcatctacct ggaagaaggc gatatcgccg agatccgtcg cgacagcgtg cgcctgtggg atgtccaggg caacgacgtg cagcgcgaga ccgtgcagta ccacgaaggc gccgaagccg ccgacaaggg cgagtaccgg cacttcatgc tcaaggaaat ccacgagcag cccagcgtcg tgcagcgcac cctggaaagg ccgcctcggg cagaaccagg tactggtgga aagcttcggt ccgcagcggc ggagctgttc gccaaggtcc gcaacgtgca gatcgtcgcc tgcggcacca gttacacgcc ggcatggtcg cccgttactg gctggaaagc tgaccgggat ncctgccagg tggagtcnnn gcgagtnnct acngcaaggt cgcgngcagc cggattgcnn tcgtcaccat ctcccantcc ggtganaccn nnaaccccnn nntnnnnnnn cnancnngnn ntgggntncn nntccagcnn tggnnnnnan nnnnnnccna ntggnnncnn attacgaann naacccngtc nnngnga

Expected Protein Sequence

MAHHHHHHMC GIVGAIAERN ITPILIEGLK RLEYRGYDSA GVAVFDNEGR LQRCRRVGKV ASLEEGLAGT PLLGRLGIAH TRWATHGAPT EGNAHPHFSS DEVAVVHNGI IENHEPLRER LKGLGYVFTS QTDTEVIVHL LHHKLQSIGD LTLALKDAVK ELHGAYGLAV ISAAQPDRIV AARSGSPLVI GLGLGENFLA SDQLALRQVT DRFIYLEEGD IAEIRRDSVR LWDVQGNDVQ RETVQYHEGA EAADKGEYRH FMLKEIHEQP SVVQRTLEGR LGQNQVLVES FGPQAAELFA KVRNVQIVAC GTSYHAGMVA RYWLESLTGI PCQVEVASEF RYRKVAVQPD CLFVTISQSG ETADTLAALR NAKELGFLSS VAICNVATSS LVRESDLTLL TQAGPEIGVA STKAFTTQLV ALLLLTLGIG QVQKRLADGV EAELVDELRR LPTRLGEALA MNRTVEKVSE LFAEKHHTLF LGRGAQFPVA LEGALKLKEI SYIHAEAYPA GELKHGPLAL VDSDMPVVTV APNNELVEKL KSNLQEVRAR GGELVVFADE GAGIEAGEGT HVVGMPHIGD VLSPILYTIP LQLLSYHVAV LKGTDVDQPR NLAKSVTVE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg gaatcgtagg cgcgatcgcc gagcgcaaca tcacccccat ccttatcgaa ggcctcaagc gcctcgagta tcgcggctac gacagcgccg gcgtggcggt cttcgataac gaaggcaggc tgcagcgctg ccgccgggtc ggcaaggtcg ccagcctgga ggaaggcctg gccggtacgc cgctgctcgg ccgcctgggc atcgcccata cccgctgggc gacccacggc gcgccaaccg aaggcaacgc ccacccgcat ttctccagcg acgaagtggc ggtggtgcac aacggcatca tcgagaacca cgaacctctc cgtgagcggc tcaagggcct gggctacgtg ttcacctcgc agaccgacac cgaggtcatc gtccacctgc tgcaccacaa gctgcagtcc atcggcgacc tgaccctggc gctgaaggac gcggtcaagg aattgcacgg ggcctatggc ctggcagtga tcagcgcggc gcagccggac cgcatcgtcg ccgctcgcag cggcagcccg ctggtgatcg gcctcgggct tggcgagaac ttcctcgctt ccgaccaact ggcgctgcgc caggtgaccg accgcttcat ctacctggaa gaaggcgata tcgccgagat ccgtcgcgac agcgtgcgcc tgtgggatgt ccagggcaac gacgtgcagc gcgagaccgt gcagtaccac gaaggcgccg aagccgccga caagggcgag taccggcact tcatgctcaa ggaaatccac gagcagccca gcgtcgtgca gcgcaccctg gaaggccgcc tcgggcagaa ccaggtactg gtggaaagct tcggtccgca ggcggcggag ctgttcgcca aggtccgcaa cgtgcagatc gtcgcctgcg gcaccagtta ccacgccggc atggtcgccc gttactggct ggaaagcctg accgggattc cctgccaggt ggaggtcgcc agcgagttcc gctaccgcaa ggtcgcggtg cagccggatt gcctgttcgt caccatctcc cagtccggtg agaccgccga caccctggct gccctgcgca acgccaagga gctgggcttc ctctccagcg tggcgatctg caacgttgcc accagttcgc tggtgcgcga atccgacctg accctgctga cccaggccgg cccggaaatc ggcgtggcct cgaccaaggc cttcaccacc cagttggtcg ccctgctgct gctgaccctg ggcatcggcc aggtgcagaa gcgcctggcc gatggcgtcg aagccgagct ggtggacgaa ctgcgtcgcc tgccgacccg gctgggcgag gcgctggcga tgaaccggac ggtggagaag gtttccgagc tgttcgccga gaagcaccac accctgttcc tcggccgtgg cgcgcagttc ccggtggccc tggaaggcgc gctgaagctc aaggaaattt cctatatcca tgccgaagcc tacccggccg gcgaactcaa gcatggcccg ctggcgctgg tggacagcga catgccggtg gttaccgtgg cgccgaacaa cgagctggtg gagaagctca agtcgaacct gcaggaagtc cgcgcccgcg gcggcgaact ggtggtgttc gccgacgaag gcgcgggcat cgaggctggc gagggcaccc atgtggtggg catgccgcac atcggcgacg tgctctcgcc gatcctctac accatcccgt tgcaattgct ctcgtaccat gtcgccgtgc tcaagggcac cgacgtcgac cagccgcgca acctggccaa gtcggtcacc gtcgagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t