SSGCID: Target Page

PsaeA.17914.a

2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-) (5-demethoxyubiquinone hydroxylase) (DMQ hydroxylase)

CENTER ID:	PsaeA.17914.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17914.a.B1.GE38370	full length		1	215

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.686
PGAT:	pseudomonas:PA0655
RefSeq:	NP_249346.1
UniProt:	Q9I5R6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSADRHYSPI DRFLLQADSA LRTLLPFSGQ PARPSPAIVE PDGELSEEDT RHIAGLMRIN HTGEVCAQAL YQGQSLTAKL PEVREAMEEA AEEEIDHLAW CEQRIRQLGS RPSVLNPIFY GLSFGVGAAA GLVSDRVSLG FVAATEDQVC KHLDEHLAQI PQEDRKSRAI LEQMRIDEEQ HSSNALAAGG LRFPAPVKLG MSLLAKVMTK STYRI

NT Sequence

atgtccgccg accgccacta ttcgcccatc gaccgtttcc tgctgcaggc cgactccgcc ctgcgcaccc tgctgccgtt cagcggccag ccggcgcgac cgtcgccggc catcgtggaa cccgatggcg aactgagcga ggaagacacc cgccatatcg ccggcctgat gcgcatcaac cacaccggcg aggtctgcgc ccaggcgctg taccagggcc agtcgctgac cgccaagctg cccgaggtcc gcgaggcgat ggaggaagcc gccgaggagg agatcgacca cctggcctgg tgcgagcaac gcatccgcca gttgggcagc cgtcccagcg tcctcaatcc gatcttctac ggcctgtcct tcggcgtcgg tgccgccgcc gggctggtca gcgaccgggt cagcctgggc ttcgtcgccg ccaccgaaga ccaggtgtgc aagcatctgg acgaacacct ggcgcagatt ccccaggagg atcggaaatc ccgtgccatc ctcgagcaga tgcgtatcga cgaggaacag cattcgagca acgccctggc cgccggcggc ctgcgcttcc cggcaccggt gaagctgggc atgagcctgc tggcgaaggt gatgaccaag agcacctacc gcatctga

Details for PsaeA.17914.a.B1.GE38370

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	25kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS ADRHYSPIDR FLLQADSALR TLLPFSGQPA RPSPAIVEPD GELSEEDTRH IAGLMRINHT GEVCAQALYQ GQSLTAKLPE VREAMEEAAE EEIDHLAWCE QRIRQLGSRP SVLNPIFYGL SFGVGAAAGL VSDRVSLGFV AATEDQVCKH LDEHLAQIPQ EDRKSRAILE QMRIDEEQHS SNALAAGGLR FPAPVKLGMS LLAKVMTKST YRI

Validated NT Sequence

gnnnntccnn tagannnttt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgtccgcc gaccgccact attcgcccat cgaccgtttc ctgctgcagg ccgactccgc cctgcgcacc ctgctgccgt tcagcggcca gccggcgcga ccgtcgccgg ccatcgtgga acccgatggc gaactgagcg aggaagacac ccgccatatc gccggcctga tgcgcatcaa ccacaccggc gaggtctgcg cccaggcgct gtaccagggc cagtcgctga ccgccaagct gcccgaggtc cgcgaggcga tggaggaagc cgccgaggag gagatcgacc acctggcctg gtgcgagcaa cgcatccgcc agttgggcag ccgtcccagc gtcctcaatc cgatcttcta cggcctgtcc ttcggcgtcg gtgccgccgc cgggctggtc agcgaccggg tcagcctggg cttcgtcgcc gccaccgaag accaggtgtg caagcatctg gacgaacacc tggcgcagat tccccaggag gatcggaaat cccgtgccat cctcgagcag atgcgtatcg acgaggaaca gcattcgagc aacgccctgg ccgccggcgg cctgcgcttc ccggcaccgg tgaagctggg catgagcctg ctggcgaagg tgatgaccaa gagcacctac cgcatctaag tgagtaagat aggatccggc tgctacagac cccccaaaag ganncagnnn nnnnnccccc cccttttttt ttttcttttg atattcctcc ctttcttccc ggnncaagtc attgagtcat cagaaactaa ctctccgccc anccccccnn cct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg ccgaccgcca ctattcgccc atcgaccgtt tcctgctgca ggccgactcc gccctgcgca ccctgctgcc gttcagcggc cagccggcgc gaccgtcgcc ggccatcgtg gaacccgatg gcgaactgag cgaggaagac acccgccata tcgccggcct gatgcgcatc aaccacaccg gcgaggtctg cgcccaggcg ctgtaccagg gccagtcgct gaccgccaag ctgcccgagg tccgcgaggc gatggaggaa gccgccgagg aggagatcga ccacctggcc tggtgcgagc aacgcatccg ccagttgggc agccgtccca gcgtcctcaa tccgatcttc tacggcctgt ccttcggcgt cggtgccgcc gccgggctgg tcagcgaccg ggtcagcctg ggcttcgtcg ccgccaccga agaccaggtg tgcaagcatc tggacgaaca cctggcgcag attccccagg aggatcggaa atcccgtgcc atcctcgagc agatgcgtat cgacgaggaa cagcattcga gcaacgccct ggccgccggc ggcctgcgct tcccggcacc ggtgaagctg ggcatgagcc tgctggcgaa ggtgatgacc aagagcacct accgcatctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat