PsaeA.17908.a

Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (EC 2.1.1.163) (EC 2.1.1.201) (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase) (Demethylmenaquinone methyltransferase)

CENTER ID: PsaeA.17908.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17908.a.B1.GE38368 full length 1 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5307
PGAT: pseudomonas:PA5063
RefSeq: NP_253750.1
UniProt: Q9HUC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNDPRKGADA EPTTHFGYQN VPESQKAKKV AEVFHSVAAK YDLMNDLMSG GIHRLWKRFT IELSGVRSGN RVLDIAGGTG DLTRQFSRLV GPTGEVVLAD INASMLKVGR DKLLDKGVSG NVSFVQADAE KLPFPDNHFD CVTIAFGLRN VTHKDEAIRS MLRVLKPGGR LLVLEFSKPS SNLLSKAYDA YSFSLLPLMG KLVTNDSESY RYLAESIRMH PDQETLKAMM VEAGFDRVTY HNMTGGIVAL HRGIKP
NT Sequence
atgaacgatc cccgcaaagg cgccgacgcc gaacccacca cccacttcgg ctaccagaac gttccggaaa gccagaaggc gaagaaggtc gccgaggtat ttcactcggt ggcagccaag tacgacctga tgaacgacct gatgtccggt ggcatccatc gcctgtggaa gcgcttcacc atcgagctgt ccggcgtgcg cagcgggaac cgcgtgctcg acatcgccgg cggcaccggc gacctgaccc ggcagttctc gcgcctggtc ggcccgaccg gcgaggtggt gctggccgac atcaacgcct cgatgctcaa ggtcggccgc gacaagctgc tcgacaaggg cgtctccggc aacgtcagct tcgtccaggc ggacgccgag aagctgccct tcccggacaa ccacttcgac tgcgtgacca tcgccttcgg cctgcgcaac gtcacccaca aggacgaagc gatccgctcc atgctgcgcg tgctcaagcc cggtggccgc ctgctggtgc tggagttctc caagccgagc agcaacctgc tgtccaaggc ctacgacgcc tattcgttca gcctgctgcc gctgatgggc aagctggtca ccaacgactc cgagagctat cgctacctgg ccgagtcgat ccgcatgcac cccgaccagg aaaccctgaa ggcgatgatg gtggaagccg gcttcgaccg cgtgacctac cacaacatga ccggcggcat cgtcgccctg caccgcggta tcaagcctta a
Details for PsaeA.17908.a.B1.GE38368
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN DPRKGADAEP TTHFGYQNVP ESQKAKKVAE VFHSVAAKYD LMNDLMSGGI HRLWKRFTIE LSGVRSGNRV LDIAGGTGDL TRQFSRLVGP TGEVVLADIN ASMLKVGRDK LLDKGVSGNV SFVQADAEKL PFPDNHFDCV TIAFGLRNVT HKDEAIRSML RVLKPGGRLL VLEFSKPSSN LLSKAYDAYS FSLLPLMGKL VTNDSESYRY LAESIRMHPD QETLKAMMVE AGFDRVTYHN MTGGIVALHR GIKP
Validated NT Sequence
gnnnnnncct ctagaatnat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg atccccgcaa aggcgccgac gccgaaccca ccacccactt cggctaccag aacgttccgg aaagccagaa ggcgaagaag gtcgccgagg tatttcactc ggtggcagcc aagtacgacc tgatgaacga cctgatgtcc ggtggcatcc atcgcctgtg gaagcgcttc accatcgagc tgtccggcgt gcgcagcggg aaccgcgtgc tcgacatcgc cggcggcacc ggcgacctga cccggcagtt ctcgcgcctg gtcggcccga ccggcgaggt ggtgctggcc gacatcaacg cctcgatgct caaggtcggc cgcgacaagc tgctcgacaa gggcgtctcc ggcaacgtca gcttcgtcca ggcggacgcc gagaagctgc ccttcccgga caaccacttc gactgcgtga ccatcgcctt cggcctgcgc aacgtcaccc acaaggacga agcgatccgc tccatgctgc gcgtgctcaa gcccggtggc cgcctgctgg tgctggagtt ctccaagccg agcagcaacc tgctgtccaa ggcctacgac gcctattcgt tcagcctgct gccgctgatg ggcaagctgg tcaccaacga ctccgagagc tatcgctacc tggccgagtc gatccgcatg caccccgacc aggaaaccct gaaggcgatg atggtggaag ccggcttcga ccgcgtgacc taccacaaca tgaccggcgg catcgtcgcc ctgcaccgcg gtatcaagcc ttaagtgagt aagataggat ccggnnnnnn nnccccccac nnnnnnnann naagaanann annnnnnnnn nnggcnctgc gcancgctcc tggaaaagan gcccngcnna aatcaangga gcgggtgttc nacgcccncc cgcgcntcgg catcagaana tgggcgcnat cgatcncgct acnccggctg nnnncntctg gctgtccggg caaccattag nnatcctgnc aatctatntn catactncgn ntgcnncata tgagtcnctn cgccngncgn ctnnnannaa atannacngn ncactnttct tcccagcggn nnnnnna
Expected Protein Sequence
MAHHHHHHMN DPRKGADAEP TTHFGYQNVP ESQKAKKVAE VFHSVAAKYD LMNDLMSGGI HRLWKRFTIE LSGVRSGNRV LDIAGGTGDL TRQFSRLVGP TGEVVLADIN ASMLKVGRDK LLDKGVSGNV SFVQADAEKL PFPDNHFDCV TIAFGLRNVT HKDEAIRSML RVLKPGGRLL VLEFSKPSSN LLSKAYDAYS FSLLPLMGKL VTNDSESYRY LAESIRMHPD QETLKAMMVE AGFDRVTYHN MTGGIVALHR GIKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg atccccgcaa aggcgccgac gccgaaccca ccacccactt cggctaccag aacgttccgg aaagccagaa ggcgaagaag gtcgccgagg tatttcactc ggtggcagcc aagtacgacc tgatgaacga cctgatgtcc ggtggcatcc atcgcctgtg gaagcgcttc accatcgagc tgtccggcgt gcgcagcggg aaccgcgtgc tcgacatcgc cggcggcacc ggcgacctga cccggcagtt ctcgcgcctg gtcggcccga ccggcgaggt ggtgctggcc gacatcaacg cctcgatgct caaggtcggc cgcgacaagc tgctcgacaa gggcgtctcc ggcaacgtca gcttcgtcca ggcggacgcc gagaagctgc ccttcccgga caaccacttc gactgcgtga ccatcgcctt cggcctgcgc aacgtcaccc acaaggacga agcgatccgc tccatgctgc gcgtgctcaa gcccggtggc cgcctgctgg tgctggagtt ctccaagccg agcagcaacc tgctgtccaa ggcctacgac gcctattcgt tcagcctgct gccgctgatg ggcaagctgg tcaccaacga ctccgagagc tatcgctacc tggccgagtc gatccgcatg caccccgacc aggaaaccct gaaggcgatg atggtggaag ccggcttcga ccgcgtgacc taccacaaca tgaccggcgg catcgtcgcc ctgcaccgcg gtatcaagcc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat