PsaeA.17906.a

50S ribosomal protein L15

CENTER ID: PsaeA.17906.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17906.a.B1.GE38366 full length 1 144
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4445
PGAT: pseudomonas:PA4244
RefSeq: NP_252934.1
UniProt: Q9HWF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLNDLRSAP GARREKHRPG RGIGSGLGKT GGRGHKGLTS RSGGKVAPGF EGGQQPLHRR LPKFGFVSLK AMDRAEVRTS ELAKVEGDVV SLQTLKDANL INQHVQRVKV MLSGEVGRAV TLKGIAATKG ARAAIEAAGG KFED
NT Sequence
atgcaactga acgatctgcg ttccgcgccg ggtgcccgtc gcgaaaagca ccgtccgggc cgtggcatcg gtagcggtct gggcaagacc ggtggccgtg gtcacaaagg tctgacctcc cgctccggtg gcaaggtcgc tccgggcttc gagggtggtc aacagcctct gcaccgtcgc ctgccgaagt tcggcttcgt ttccctgaag gctatggatc gtgctgaagt acgcacctcc gagctggcca aggtcgaagg cgacgtcgtc agcctgcaga ccctgaagga tgccaacctg atcaaccagc acgtacagcg cgtcaaagtc atgctgtccg gcgaagttgg tcgtgcggtc accctgaaag gtatcgccgc caccaagggt gcgcgtgcgg ctatcgaagc agctggcggt aaattcgagg actaa
Details for PsaeA.17906.a.B1.GE38366
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 16kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ LNDLRSAPGA RREKHRPGRG IGSGLGKTGG RGHKGLTSRS GGKVAPGFEG GQQPLHRRLP KFGFVSLKAM DRAEVRTSEL AKVEGDVVSL QTLKDANLIN QHVQRVKVML SGEVGRAVTL KGIAATKGAR AAIEAAGGKF ED
Validated NT Sequence
cctctagnnt nttttgttta ntttaagaag gagatatacc atggctcacc accaccacca ccatatgcaa ctgaacgatc tgcgttccgc gccgggtgcc cgtcgcgaaa agcaccgtcc gggccgtggc atcggtagcg gtctgggcaa gaccggtggc cgtggtcaca aaggtctgac ctcccgctcc ggtggcaagg tcgctccggg cttcgagggt ggtcaacagc ctctgcaccg tcgcctgccg aagttcggct tcgtttccct gaaggctatg gatcgtgctg aagtacgcac ctccgagctg gccaaggtcg aaggcgacgt cgtcagcctg cagaccctga aggatgccaa cctgatcaac cagcacgtac agcgcgtcaa agtcatgctg tccggcgaag ttggtcgtgc ggtcaccctg aaaggtatcg ccgccaccaa gggtgcgcgt gcggctatcg aagcagctgg cggtaaattc gaggactaag tgagtaagat aggatccggc tgctaacaag gccccgaaag gannnngngn naanncccnn nnaaaaaaaa tagtaaaact gctcgcnntc gggcccgcct atgtccagtg ccgnattacc tatcatcgac ggagtgcgcg cctcgagaca tccagatcan ngtggcagac antgtgagac agcgaagtct cccgcccaga gcttngcngc gagccgtcgn ggacgancgt gtgagcccgc anatcaagag accantgccc nngncagagc cgcagtagag nactaagtcc atgnacatac cacgacatcn gagtacgttg cnnanntccc atcccgacca tntcaannna cgnnntcngc nnnnncgtng nncatacgnn nnatcacnan nntganntcg acgnngnagc tgatnacnan gncagcggnn tgatnntaca tnctnnnnnn nnngntngat nnnnnannna cagnnngnnn nnngaacnnn nnnngtcgng tcatncannt tcnngnnnnn tnnggnagnn tnccnnnnnn annnnannnn ggnnnccnnn nncnnnnnnc nnnnngcgac ngggttcnnc
Expected Protein Sequence
MAHHHHHHMQ LNDLRSAPGA RREKHRPGRG IGSGLGKTGG RGHKGLTSRS GGKVAPGFEG GQQPLHRRLP KFGFVSLKAM DRAEVRTSEL AKVEGDVVSL QTLKDANLIN QHVQRVKVML SGEVGRAVTL KGIAATKGAR AAIEAAGGKF ED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac tgaacgatct gcgttccgcg ccgggtgccc gtcgcgaaaa gcaccgtccg ggccgtggca tcggtagcgg tctgggcaag accggtggcc gtggtcacaa aggtctgacc tcccgctccg gtggcaaggt cgctccgggc ttcgagggtg gtcaacagcc tctgcaccgt cgcctgccga agttcggctt cgtttccctg aaggctatgg atcgtgctga agtacgcacc tccgagctgg ccaaggtcga aggcgacgtc gtcagcctgc agaccctgaa ggatgccaac ctgatcaacc agcacgtaca gcgcgtcaaa gtcatgctgt ccggcgaagt tggtcgtgcg gtcaccctga aaggtatcgc cgccaccaag ggtgcgcgtg cggctatcga agcagctggc ggtaaattcg aggactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat