PsaeA.17905.a

Thiamine-monophosphate kinase (TMP kinase) (Thiamine-phosphate kinase) (EC 2.7.4.16)

CENTER ID: PsaeA.17905.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17905.a.B2.GE38365 mature protein 27 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4242
PGAT: pseudomonas:PA4051
RefSeq: NP_252740.1
UniProt: Q9HWX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGEFELIRRF FAAAACAAPA ADVALGIGDD CALLAPPAGE QLAVSTDTLV EGVHFPAGCD PFLLAQRALA VSASDLAAMG AAPLAFTLAL TLPQADAEWL QGFARGLDAM ARQCGLALVG GDTTRGPLSM TLTVFGRVPA GQALTRAGAR PGDLLCVGGP LGEAGAALEL VLERRSAPAE VAEPLLARYW TPAPQFGLGL ALRGKASAAL DISDGLLADC GHIARASGVA LLVECQRLQA SAALSGLLAG EEALRQQLAA GDDYVLVFTL PPEYLGEIRA AWPAMAVIGR VEAGQGVHLL DADGKELIPA AAGYQHFGTQ RD
NT Sequence
atgggtgagt tcgagctgat ccgccgcttc ttcgccgcgg ccgcctgcgc ggcgccggcg gcggacgtgg ccctcggcat cggcgacgac tgcgccctgc tggcgccgcc agccggcgaa caactggcgg tctccaccga taccctggtg gaaggcgtgc attttcccgc cggttgcgac cctttccttc ttgcccagcg cgccctggcg gtctccgcca gcgacctggc ggcgatgggc gccgcgcccc tggccttcac cctggccctg accttgccgc aggccgacgc cgagtggttg cagggcttcg cccgcggcct cgacgccatg gcccggcagt gcggcctggc cctggtcggc ggcgacacca cgcgcggccc gctgagcatg accctgaccg tattcggcag ggtgccggcc ggccaggcgc tgacccgcgc cggtgcgcgc ccgggcgacc tgctctgcgt cgggggcccg ctcggcgagg ctggagcggc gctggaactg gtgctggagc gaagatcggc gcccgccgag gtggccgaac cgctgctggc gcgctactgg acgccggccc cgcaattcgg tctgggcctc gccctgcgcg gcaaggccag cgccgccctg gacatctccg acgggctgct cgccgactgc gggcatatcg cccgcgcttc cggcgttgcc ctgttggtcg aatgccagcg cttgcaggca agcgccgcgt tgagcggcct gctcgccggc gaggaagcct tgcgccagca actggccgcc ggcgacgact acgtgctggt tttcaccctg ccgcctgagt accttggcga gattcgcgcc gcgtggccgg ccatggccgt catcggccgg gtcgaggcgg gacagggggt gcacctgctc gacgccgacg gaaaggaact cataccggcc gcggccggtt accaacattt cggaacccaa cgtgactga
Details for PsaeA.17905.a.B2.GE38365
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMI GDDCALLAPP AGEQLAVSTD TLVEGVHFPA GCDPFLLAQR ALAVSASDLA AMGAAPLAFT LALTLPQADA EWLQGFARGL DAMARQCGLA LVGGDTTRGP LSMTLTVFGR VPAGQALTRA GARPGDLLCV GGPLGEAGAA LELVLERRSA PAEVAEPLLA RYWTPAPQFG LGLALRGKAS AALDISDGLL ADCGHIARAS GVALLVECQR LQASAALSGL LAGEEALRQQ LAAGDDYVLV FTLPPEYLGE IRAAWPAMGR HRPGXXREQG VHLLDADGRN SYRPRPVTXF GPT
Validated NT Sequence
tnctctanat attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgat cggcgacgac tgcgccctgc tggcgccgcc agccggcgaa caactggcgg tctccaccga taccctggtg gaaggcgtgc attttcccgc cggttgcgac cctttccttc ttgcccagcg cgccctggcg gtctccgcca gcgacctggc ggcgatgggc gccgcgcccc tggccttcac cctggccctg accttgccgc aggccgacgc cgagtggttg cagggcttcg cccgcggcct cgacgccatg gcccggcagt gcggcctggc cctggtcggc ggcgacacca cgcgcggccc gctgagcatg accctgaccg tattcggcag ggtgccggcc ggccaggcgc tgacccgcgc cggtgcgcgc ccgggcgacc tgctctgcgt cgggggcccg ctcggcgagg ctggagcggc gctggaactg gtgctggagc gaagatcggc gcccgccgag gtggccgaac cgctgctggc gcgctactgg acgccggccc cgcaattcgg tctgggcctc gccctgcgcg gcaaggccag cgccgccctg gacatctccg acgggctgct cgccgactgc gggcatatcg cccgcgcttc cggcgttgcc ctgttggtcg aatgccagcg cttgcaggca agcgccgcgt tgagcggcct gctcgccggc gaggaagcct tgcgccagca actggccgcc ggcgacgact acgtgctggt tttcaccctg ccgcctgagt accttggcga gattcgcgcc gcgtggccgg ccatgggccg tcatcggccg ggtcnnnggc gggaacaggg ggtgcacctg ctcgacgccg acggaaggaa ctcataccgg ccgcggccgg ttacnnattt cggacccacg tgactantga gtanatnnnt cnntnnnnnn nnnnnaannn nnannnnncn tngngnnttc gggaaggtgc tcntcaannn nntatnnnnn gagntcngtc ncnncgnnnn gacagctnnn nnttnnncgn nngnancnnn nnnctagcaa cntnnnnncg ggannganna
Expected Protein Sequence
MAHHHHHHIG DDCALLAPPA GEQLAVSTDT LVEGVHFPAG CDPFLLAQRA LAVSASDLAA MGAAPLAFTL ALTLPQADAE WLQGFARGLD AMARQCGLAL VGGDTTRGPL SMTLTVFGRV PAGQALTRAG ARPGDLLCVG GPLGEAGAAL ELVLERRSAP AEVAEPLLAR YWTPAPQFGL GLALRGKASA ALDISDGLLA DCGHIARASG VALLVECQRL QASAALSGLL AGEEALRQQL AAGDDYVLVF TLPPEYLGEI RAAWPAMAVI GRVEAGQGVH LLDADGKELI PAAAGYQHFG TQRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcggcg acgactgcgc cctgctggcg ccgccagccg gcgaacaact ggcggtctcc accgataccc tggtggaagg cgtgcatttt cccgccggtt gcgacccttt ccttcttgcc cagcgcgccc tggcggtctc cgccagcgac ctggcggcga tgggcgccgc gcccctggcc ttcaccctgg ccctgacctt gccgcaggcc gacgccgagt ggttgcaggg cttcgcccgc ggcctcgacg ccatggcccg gcagtgcggc ctggccctgg tcggcggcga caccacgcgc ggcccgctga gcatgaccct gaccgtattc ggcagggtgc cggccggcca ggcgctgacc cgcgccggtg cgcgcccggg cgacctgctc tgcgtcgggg gcccgctcgg cgaggctgga gcggcgctgg aactggtgct ggagcgaaga tcggcgcccg ccgaggtggc cgaaccgctg ctggcgcgct actggacgcc ggccccgcaa ttcggtctgg gcctcgccct gcgcggcaag gccagcgccg ccctggacat ctccgacggg ctgctcgccg actgcgggca tatcgcccgc gcttccggcg ttgccctgtt ggtcgaatgc cagcgcttgc aggcaagcgc cgcgttgagc ggcctgctcg ccggcgagga agccttgcgc cagcaactgg ccgccggcga cgactacgtg ctggttttca ccctgccgcc tgagtacctt ggcgagattc gcgccgcgtg gccggccatg gccgtcatcg gccgggtcga ggcgggacag ggggtgcacc tgctcgacgc cgacggaaag gaactcatac cggccgcggc cggttaccaa catttcggaa cccaacgtga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat