PsaeA.17901.a

Peptide chain release factor 1 (RF-1)

CENTER ID: PsaeA.17901.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17901.a.B1.GE38362 full length 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4887
PGAT: pseudomonas:PA4665
RefSeq: NP_253354.1
UniProt: P42806

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKASLLKKLD VLSDRYEELT ALLGDAEVIS DQTRFRAYSR EYAEVEPVIL AFRDYRKVQA DLEGAQALLK DSDPELRDLA EEEVAEARGR LAALGDSLQR MLLPKDPNDS RNVFLEIRAG TGGDEAAIFS GDLFRMYSRY AERQGWRIET LSENEGEHGG YKEVIARVEG DNVYAKLKFE SGAHRVQRVP ETESQGRIHT SACTVAVLPE PDEQAAIEIN PADLRVDTYR SSGAGGQHVN KTDSAVRITH IPSGIVVECQ EERSQHKNRA KAMAWLAAKL NDQQQAAAQQ AIASTRKLLV GSGDRSERIR TYNFPQGRVT DHRINLTLYS LGEVMEGAVE QVIEPLLQEY QADQLAALGD
NT Sequence
ATGAAAGCTT CTCTGCTGAA AAAGCTGGAT GTCCTCAGCG ATCGCTACGA AGAACTGACG GCGCTGCTCG GCGACGCCGA GGTGATCAGT GACCAGACCC GCTTCCGCGC CTATTCCCGC GAGTACGCCG AGGTCGAACC GGTGATCCTG GCGTTCCGCG ACTACCGCAA GGTGCAGGCC GACCTCGAGG GCGCCCAGGC GTTGCTCAAG GACAGCGACC CGGAGTTGCG CGACCTCGCC GAGGAGGAGG TCGCCGAAGC GCGCGGCCGC CTCGCCGCCC TCGGCGACAG CCTGCAGCGC ATGCTGCTGC CGAAGGATCC CAACGACAGC CGCAACGTGT TCCTGGAGAT CCGTGCCGGC ACCGGTGGCG ACGAGGCGGC GATCTTCTCC GGCGACCTGT TCCGCATGTA TTCGCGCTAC GCCGAGCGCC AGGGCTGGCG GATCGAGACG CTGTCGGAGA ACGAGGGCGA GCACGGTGGC TACAAGGAAG TGATTGCCCG GGTCGAGGGC GACAACGTCT ACGCCAAGCT CAAGTTCGAG TCCGGCGCGC ACCGCGTGCA GCGGGTGCCG GAAACCGAAT CCCAGGGCCG GATCCACACT TCCGCCTGCA CCGTCGCGGT GCTGCCGGAG CCGGACGAGC AGGCAGCGAT CGAGATCAAC CCGGCCGACC TGCGGGTGGA CACCTACCGT TCCTCCGGTG CCGGCGGCCA GCACGTCAAC AAGACCGACT CGGCGGTGCG CATCACCCAC ATTCCCAGCG GCATCGTGGT CGAGTGCCAG GAAGAGCGCT CGCAGCACAA GAACCGCGCC AAGGCCATGG CCTGGCTGGC GGCCAAGCTC AACGACCAGC AGCAGGCCGC GGCGCAGCAG GCGATCGCCA GCACGCGCAA GCTGCTGGTG GGCTCGGGCG ACCGCTCGGA GCGCATCCGT ACCTACAACT TCCCGCAAGG GCGGGTCACC GACCATCGCA TCAACCTCAC CCTGTACTCC CTGGGCGAGG TGATGGAGGG CGCGGTGGAA CAGGTGATCG AGCCGCTGCT GCAGGAATAC CAGGCCGATC AACTGGCGGC CCTGGGCGAC TGA
Details for PsaeA.17901.a.B1.GE38362
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 41kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMK ASLLKKLDVL SDRYEELTAL LGDAEVISDQ TRFRAYSREY AEVEPVILAF RDYRKVQADL EGAQALLKDS DPELRDLAEE EVAEARGRLA ALGDSLQRML LPKDPNDSRN VFLEIRAGTG GDEAAIFSGD LFRMYSRYAE RQGWRIETLS ENEGEHGGYK EVIARVEGDN VYAKLKFESG AHRVQRVPET ESQGRIHTSA CTVAVLPEPD EQAAIEINPA DLRVDTYRSS GAGGQHVNKT DSAVRITHIP SGIVVECQEE RSQHKNRAKA MAWLAAKLND QQQAAAQQAI ASTRKLLVGS GDRSERIXLH FPQGXVTTSH HSPCTPWXRX WRXRXNRXSX RXCXXXXIXG XXXXXXXXXX XXXXXXXXXX XXXXRXXXXX X
Validated NT Sequence
anttnnctag antattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat gaaagcttct ctgctgaaaa agctggatgt cctcagcgat cgctacgaag aactgacggc gctgctcggc gacgccgagg tgatcagtga ccagacccgc ttccgcgcct attcccgcga gtacgccgag gtcgaaccgg tgatcctggc gttccgcgac taccgcaagg tgcaggccga cctcgagggc gcccaggcgt tgctcaagga cagcgacccg gagttgcgcg acctcgccga ggaggaggtc gccgaagcgc gcggccgcct cgccgccctc ggcgacagcc tgcagcgcat gctgctgccg aaggatccca acgacagccg caacgtgttc ctggagatcc gtgccggcac cggtggcgac gaggcggcga tcttctccgg cgacctgttc cgcatgtatt cgcgctacgc cgagcgccag ggctggcgga tcgagacgct gtcggagaac gagggcgagc acggtggcta caaggaagtg attgcccggg tcgagggcga caacgtctac gccaagctca agttcgagtc cggcgcgcac cgcgtgcagc gggtgccgga aaccgaatcc cagggccgga tccacacttc cgcctgcacc gtcgcggtgc tgccggagcc ggacgagcag gcagcgatcg agatcaaccc ggccgacctg cgggtggaca cctaccgttc ctccggtgcc ggcggccagc acgtcaacaa gaccgactcg gcggtgcgca tcacccacat tcccagcggc atcgtggtcg agtgccagga agagcgctcg cagcacaaga accgcgccaa ggccatggcc tggctggcgg ccaagctcaa cgaccagcag caggccgcgg cgcagcaggc gatcgccagc acgcgcaagc tgctggtggg ctcgggcgac cgctcggagc gcatcntact acacttcccg caagggnggg tcacgacntc gcatcactca ccctgtactc cctggnnnag gngatggagg gnncggngga acaggtnatc ganccgctnc tgcngantnc nnnnnatcan tggnnnntgn nnnntnnnnn anagnnnnnn nnnnnnncnn nnnnnnnnnc nnnnnnnnan nangannann tnnnnnagan nnanangana nnngnnnga
Expected Protein Sequence
MAHHHHHHMK ASLLKKLDVL SDRYEELTAL LGDAEVISDQ TRFRAYSREY AEVEPVILAF RDYRKVQADL EGAQALLKDS DPELRDLAEE EVAEARGRLA ALGDSLQRML LPKDPNDSRN VFLEIRAGTG GDEAAIFSGD LFRMYSRYAE RQGWRIETLS ENEGEHGGYK EVIARVEGDN VYAKLKFESG AHRVQRVPET ESQGRIHTSA CTVAVLPEPD EQAAIEINPA DLRVDTYRSS GAGGQHVNKT DSAVRITHIP SGIVVECQEE RSQHKNRAKA MAWLAAKLND QQQAAAQQAI ASTRKLLVGS GDRSERIRTY NFPQGRVTDH RINLTLYSLG EVMEGAVEQV IEPLLQEYQA DQLAALGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cttctctgct gaaaaagctg gatgtcctca gcgatcgcta cgaagaactg acggcgctgc tcggcgacgc cgaggtgatc agtgaccaga cccgcttccg cgcctattcc cgcgagtacg ccgaggtcga accggtgatc ctggcgttcc gcgactaccg caaggtgcag gccgacctcg agggcgccca ggcgttgctc aaggacagcg acccggagtt gcgcgacctc gccgaggagg aggtcgccga agcgcgcggc cgcctcgccg ccctcggcga cagcctgcag cgcatgctgc tgccgaagga tcccaacgac agccgcaacg tgttcctgga gatccgtgcc ggcaccggtg gcgacgaggc ggcgatcttc tccggcgacc tgttccgcat gtattcgcgc tacgccgagc gccagggctg gcggatcgag acgctgtcgg agaacgaggg cgagcacggt ggctacaagg aagtgattgc ccgggtcgag ggcgacaacg tctacgccaa gctcaagttc gagtccggcg cgcaccgcgt gcagcgggtg ccggaaaccg aatcccaggg ccggatccac acttccgcct gcaccgtcgc ggtgctgccg gagccggacg agcaggcagc gatcgagatc aacccggccg acctgcgggt ggacacctac cgttcctccg gtgccggcgg ccagcacgtc aacaagaccg actcggcggt gcgcatcacc cacattccca gcggcatcgt ggtcgagtgc caggaagagc gctcgcagca caagaaccgc gccaaggcca tggcctggct ggcggccaag ctcaacgacc agcagcaggc cgcggcgcag caggcgatcg ccagcacgcg caagctgctg gtgggctcgg gcgaccgctc ggagcgcatc cgtacctaca acttcccgca agggcgggtc accgaccatc gcatcaacct caccctgtac tccctgggcg aggtgatgga gggcgcggtg gaacaggtga tcgagccgct gctgcaggaa taccaggccg atcaactggc ggccctgggc gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat