SSGCID: Target Page

PsaeA.17894.a

30S ribosomal protein S2

CENTER ID:	PsaeA.17894.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17894.a.B1.GE38359	full length		1	246

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3825
PGAT:	pseudomonas:PA3656
RefSeq:	NP_252346.1
UniProt:	O82850

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQVNMRDML KAGVHFGHQT RYWNPKMGKF IFGARNKIHI INLEKTLPMF NEALTFVERL AAGKNKILFV GTKRSAGKIV REEAARCGMP YVDHRWLGGM LTNYKTIRQS IKRLRDLETQ SQDGTFDKLT KKEALMRSRD LEKLERSLGG IKDMGGLPDA LFVIDVDHER IAITEANKLG IPVIGVVDTN SSPEGVDYVI PGNDDAIRAV QLYLNSMAEA VIRGKQGAAT SADEFVEEAP AESAEG

NT Sequence

atgtcccaag tcaacatgcg cgatatgctg aaggccggtg tgcacttcgg ccaccagacc cgttactgga acccgaaaat gggcaagttc attttcggcg cgcgcaacaa gatccatatc atcaacctcg aaaagaccct gccgatgttc aacgaggccc tgaccttcgt tgagcgcctg gccgctggca agaacaagat cctgttcgtc ggcaccaagc gttccgccgg caagatcgtt cgcgaggaag ccgctcgttg cggtatgccg tacgtcgacc atcgctggct gggcggcatg ctcaccaact acaagaccat ccgtcagtcg atcaagcgcc tgcgcgacct ggaaacccag tcccaggacg gcaccttcga caagctgacc aagaaagaag ccctgatgcg cagccgtgac ctcgagaagc tcgagcgcag cctgggcggc atcaaggaca tgggcggcct gccggacgct ctgttcgtga tcgacgtcga ccacgagcgc atcgccatca ccgaagccaa caagctgggc atcccggtca tcggcgttgt cgataccaac agcagcccgg aaggcgtcga ctatgtaatt cccggtaacg atgatgccat ccgcgccgtg caactctacc tgaactcgat ggccgaagcg gtaatccgcg gcaagcaggg cgctgccacc agcgccgacg agttcgtcga ggaagcgccg gccgaatccg ccgaaggctg a

Details for PsaeA.17894.a.B1.GE38359

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	28kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS QVNMRDMLKA GVHFGHQTRY WNPKMGKFIF GARNKIHIIN LEKTLPMFNE ALTFVERLAA GKNKILFVGT KRSAGKIVRE EAARCGMPYV DHRWLGGMLT NYKTIRQSIK RLRDLETQSQ DGTFDKLTKK EALMRSRDLE KLERSLGGIK DMGGLPDALF VIDVDHERIA ITEANKLGIP VIGVVDTNSS PEGVDYVIPG NDDAIRAVQL YLNSMAEAVI RGKQGAATSA DEFVEEAPAE SAES

Validated NT Sequence

tcnctagaat aattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgt cccaagtcaa catgcgcgat atgctgaagg ccggtgtgca cttcggccac cagacccgtt actggaaccc gaaaatgggc aagttcattt tcggcgcgcg caacaagatc catatcatca acctcgaaaa gaccctgccg atgttcaacg aggccctgac cttcgttgag cgcctggccg ctggcaagaa caagatcctg ttcgtcggca ccaagcgttc cgccggcaag atcgttcgcg aggaagccgc tcgttgcggt atgccgtacg tcgaccatcg ctggctgggc ggcatgctca ccaactacaa gaccatccgt cagtcgatca agcgcctgcg cgacctggaa acccagtccc aggacggcac cttcgacaag ctgaccaaga aagaagccct gatgcgcagc cgtgacctcg agaagctcga gcgcagcctg ggcggcatca aggacatggg cggcctgccg gacgctctgt tcgtgatcga cgtcgaccac gagcgcatcg ccatcaccga agccaacaag ctgggcatcc cggtcatcgg cgttgtcgat accaacagca gcccggaagg cgtcgactat gtaattcccg gtaacgatga tgccatccgc gccgtgcaac tctacctgaa ctcgatggcc gaagcggtaa tccgcggcaa gcagggcgct gccaccagcg ccgacgagtt cgtcgaggaa gcgccggccg aatccgccga aagctaagtg agtaagatag gatccnntnn nnnccccccc caananagaa gagantnann nnnnaaaaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccc aagtcaacat gcgcgatatg ctgaaggccg gtgtgcactt cggccaccag acccgttact ggaacccgaa aatgggcaag ttcattttcg gcgcgcgcaa caagatccat atcatcaacc tcgaaaagac cctgccgatg ttcaacgagg ccctgacctt cgttgagcgc ctggccgctg gcaagaacaa gatcctgttc gtcggcacca agcgttccgc cggcaagatc gttcgcgagg aagccgctcg ttgcggtatg ccgtacgtcg accatcgctg gctgggcggc atgctcacca actacaagac catccgtcag tcgatcaagc gcctgcgcga cctggaaacc cagtcccagg acggcacctt cgacaagctg accaagaaag aagccctgat gcgcagccgt gacctcgaga agctcgagcg cagcctgggc ggcatcaagg acatgggcgg cctgccggac gctctgttcg tgatcgacgt cgaccacgag cgcatcgcca tcaccgaagc caacaagctg ggcatcccgg tcatcggcgt tgtcgatacc aacagcagcc cggaaggcgt cgactatgta attcccggta acgatgatgc catccgcgcc gtgcaactct acctgaactc gatggccgaa gcggtaatcc gcggcaagca gggcgctgcc accagcgccg acgagttcgt cgaggaagcg ccggccgaat ccgccgaagg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat