PsaeA.17885.a
Aspartate-semialdehyde dehydrogenase (ASA dehydrogenase) (ASADH) (EC 1.2.1.11) (Aspartate-beta-semialdehyde dehydrogenase)
| CENTER ID: | PsaeA.17885.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17885.a.B1.GE38354 | full length | 1 | 370 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17885.a.B1.PS02341 | full length | 1 | 370 |
Structures
5BNT
DEPOSITED: 5/26/2015
DETERMINATION: XRay
CLONE: PsaeA.17885.a.B1.GE38354
PROTEIN: PsaeA.17885.a.B1.PS02341
Publications by SSGCID
Structural Characterization of Aspartate-Semialdehyde Dehydrogenase from Pseudomonas aeruginosa and Neisseria gonorrhoeae
Abendroth J, Dranow DM, Edwards TE, Fairman JW, Lorimer DD, Muruthi MM, Forwood JK, Teakel S
Sci Rep - 2022
volume 12, issue 1, pages 14010
PMID: 35977963; PMCID: PMC9385607
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.3269 |
| PGAT: | pseudomonas:PA3117 |
| RefSeq: | NP_251807.1 |
| UniProt: | Q51344 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRVGLIGWR GMVGSVLMQR MLEERDFDLI EPVFFTTSNV GGQGPEVGKD IAPLKDAYSI DELKTLDVIL TCQGGDYTSE VFPKLREAGW QGYWIDAASS LRMEDDAVIV LDPVNRKVID QALDAGTRNY IGGNCTVSLM LMALGGLFDA GLVEWMSAMT YQAASGAGAQ NMRELLKQMG AAHASVADDL ANPASAILDI DRKVAETLRS EAFPTEHFGA PLGGSLIPWI DKELPNGQSR EEWKAQAETN KILARFKNPI PVDGICVRVG AMRCHSQALT IKLNKDVPLT DIEGLISQHN PWVKLVPNHR EVSVRELTPA AVTGTLSVPV GRLRKLNMGS QYLGAFTVGD QLLWGAAEPL RRMLRILLER
NT Sequence
ATGAAGCGTG TAGGTCTGAT CGGTTGGCGC GGGATGGTGG GTTCCGTACT CATGCAGCGA ATGCTGGAAG AGCGGGACTT CGACCTGATC GAGCCGGTGT TCTTCACCAC GTCGAACGTC GGTGGCCAGG GCCCGGAAGT GGGCAAGGAC ATCGCTCCGC TGAAGGACGC CTACAGCATC GACGAGCTGA AGACCCTCGA CGTGATCCTG ACCTGCCAGG GCGGCGACTA CACCAGCGAA GTATTCCCCA AGCTGCGCGA GGCCGGCTGG CAGGGCTACT GGATCGATGC CGCCTCCAGT CTGCGCATGG AAGACGACGC GGTGATCGTC CTCGACCCGG TCAACCGCAA GGTCATCGAC CAGGCTCTGG ACGCCGGCAC CCGCAACTAC ATCGGCGGCA ACTGCACCGT CAGCCTGATG CTGATGGCCC TGGGCGGGCT GTTCGACGCC GGTCTGGTCG AGTGGATGAG TGCCATGACC TACCAGGCCG CCTCCGGCGC CGGCGCGCAG AACATGCGTG AACTGCTCAA GCAGATGGGC GCCGCCCATG CATCGGTGGC GGATGACCTG GCCAACCCGG CCAGCGCGAT CCTCGACATC GACCGCAAGG TGGCGGAGAC CCTGCGCAGC GAAGCCTTCC CCACCGAGCA TTTCGGCGCG CCGCTGGGCG GCAGCCTGAT CCCCTGGATC GACAAGGAAC TGCCCAACGG GCAGAGCCGC GAAGAGTGGA AGGCCCAGGC GGAGACCAAC AAGATCCTTG CCCGCTTCAA GAACCCGATC CCGGTCGACG GTATCTGCGT GCGCGTCGGC GCCATGCGTT GCCACAGCCA GGCGCTGACC ATCAAGTTGA ACAAGGATGT GCCGCTGACC GACATCGAGG GCCTGATCAG CCAGCACAAT CCCTGGGTCA AGCTGGTGCC GAACCACCGC GAGGTAAGCG TGCGCGAGCT GACCCCGGCC GCCGTCACCG GCACCCTGAG CGTTCCGGTC GGGCGCCTGC GCAAGTTGAA CATGGGCTCC CAGTATCTCG GCGCCTTCAC CGTCGGCGAC CAGTTGCTGT GGGGCGCTGC CGAGCCGCTG CGGCGCATGT TGCGCATCCT GCTGGAGCGC TGA
Details for PsaeA.17885.a.B1.GE38354
| HARVESTED ON: | 7/21/2014 |
| SEQUENCED ON: | 7/26/2014 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MVGSVLMQRM LEERDFDLIE PVFFTTSNVG GQGPEVGKDI APLKDAYSID ELKTLDVILT CQGGDYTSEV FPKLREAGWQ GYWIDAASSL RMEDDAVIVL DPVNRKVIDQ ALDAGTRNYI GGNCTVSLML MALGGLFDAG LVEWMSAMTY QAASGAGAQN MRELLKQMGA AHASVADDLA NPASAILDID RKVAETLRSE AFPTEHFGAP LGGSLIPWID KELPNGQSRE EWKAQAETNK ILARFKNPIP VDGICVRVGA MRCHSQALTI KLNKDVPLTD IEGLISQHNP WVKLVPNHRE VSVRELTPAA VTGTLSVPVG RLRKLNMGSQ YLGAFTVGDQ LLWGAAEPLR RMLRILLER
Validated NT Sequence
tagcgctcca gcaggatgcg caacatgcgc cgcagcggct cggcagcgcc ccacagcaac tggtcgccga cggtgaaggc gccgagatac tgggagccca tgttcaactt gcgcaggcgc ccgaccggaa cgctcagggt gccggtgacg gcggccgggg tcagctcgcg cacgcttacc tcgcggtggt tcggcaccag cttgacccag ggattgtgct ggctgatcag gccctcgatg tcggtcagcg gcacatcctt gttcaacttg atggtcagcg cctggctgtg gcaacgcatg gcgccgacgc gcacgcagat accgtcgacc gggatcgggt tcttgaagcg ggcaaggatc ttgttggtct ccgcctgggc cttccactct tcgcggctct gcccgttggg cagttccttg tcgatccagg ggatcaggct gccgcccagc ggcgcgccga aatgctcggt ggggaaggct tcgctgcgca gggtctccgc caccttgcgg tcgatgtcga ggatcgcgct ggccgggttg gccaggtcat ccgccaccga tgcatgggcg gcgcccatct gcttgagcag ttcacgcatg ttctgcgcgc cggcgccgga ggcggcctgg taggtcatgg cactcatcca ctcgaccaga ccggcgtcga acagcccgcc cagggccatc agcatcaggc tgacggtgca gttgccgccg atgtagttgc gggtgccggc gtccagagcc tggtcgatga ccttgcggtt gaccgggtcg aggacgatca ccgcgtcgtc ttccatgcgc agactggagg cggcatcgat ccagtagccc tgccagccgg cctcgcgcag cttggggaat acttcgctgg tgtagtcgcc gccctggcag gtcaggatca cgtcgagggt cttcagctcg tcgatgctgt aggcgtcctt cagcggagcg atgtccttgc ccacttccgg gccctggcca ccgacgttcg acgtggtgaa gaacaccggc tcgatcaggt cgaagtcccg ctcttccagc attcgctgca tgagtacgga acccaccatc ccgcgccaac cgatcagacc tacacgctt
Expected Protein Sequence
MAHHHHHHMK RVGLIGWRGM VGSVLMQRML EERDFDLIEP VFFTTSNVGG QGPEVGKDIA PLKDAYSIDE LKTLDVILTC QGGDYTSEVF PKLREAGWQG YWIDAASSLR MEDDAVIVLD PVNRKVIDQA LDAGTRNYIG GNCTVSLMLM ALGGLFDAGL VEWMSAMTYQ AASGAGAQNM RELLKQMGAA HASVADDLAN PASAILDIDR KVAETLRSEA FPTEHFGAPL GGSLIPWIDK ELPNGQSREE WKAQAETNKI LARFKNPIPV DGICVRVGAM RCHSQALTIK LNKDVPLTDI EGLISQHNPW VKLVPNHREV SVRELTPAAV TGTLSVPVGR LRKLNMGSQY LGAFTVGDQL LWGAAEPLRR MLRILLER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc gtgtaggtct gatcggttgg cgcgggatgg tgggttccgt actcatgcag cgaatgctgg aagagcggga cttcgacctg atcgagccgg tgttcttcac cacgtcgaac gtcggtggcc agggcccgga agtgggcaag gacatcgctc cgctgaagga cgcctacagc atcgacgagc tgaagaccct cgacgtgatc ctgacctgcc agggcggcga ctacaccagc gaagtattcc ccaagctgcg cgaggccggc tggcagggct actggatcga tgccgcctcc agtctgcgca tggaagacga cgcggtgatc gtcctcgacc cggtcaaccg caaggtcatc gaccaggctc tggacgccgg cacccgcaac tacatcggcg gcaactgcac cgtcagcctg atgctgatgg ccctgggcgg gctgttcgac gccggtctgg tcgagtggat gagtgccatg acctaccagg ccgcctccgg cgccggcgcg cagaacatgc gtgaactgct caagcagatg ggcgccgccc atgcatcggt ggcggatgac ctggccaacc cggccagcgc gatcctcgac atcgaccgca aggtggcgga gaccctgcgc agcgaagcct tccccaccga gcatttcggc gcgccgctgg gcggcagcct gatcccctgg atcgacaagg aactgcccaa cgggcagagc cgcgaagagt ggaaggccca ggcggagacc aacaagatcc ttgcccgctt caagaacccg atcccggtcg acggtatctg cgtgcgcgtc ggcgccatgc gttgccacag ccaggcgctg accatcaagt tgaacaagga tgtgccgctg accgacatcg agggcctgat cagccagcac aatccctggg tcaagctggt gccgaaccac cgcgaggtaa gcgtgcgcga gctgaccccg gccgccgtca ccggcaccct gagcgttccg gtcgggcgcc tgcgcaagtt gaacatgggc tcccagtatc tcggcgcctt caccgtcggc gaccagttgc tgtggggcgc tgccgagccg ctgcggcgca tgttgcgcat cctgctggag cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.17885.a.B1.PS02341
| PURIFICATION DATe: | 2/26/2015 |
| CONCENTRATION: | 58.82mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVGSVLMQRM LEERDFDLIE PVFFTTSNVG GQGPEVGKDI APLKDAYSID ELKTLDVILT CQGGDYTSEV FPKLREAGWQ GYWIDAASSL RMEDDAVIVL DPVNRKVIDQ ALDAGTRNYI GGNCTVSLML MALGGLFDAG LVEWMSAMTY QAASGAGAQN MRELLKQMGA AHASVADDLA NPASAILDID RKVAETLRSE AFPTEHFGAP LGGSLIPWID KELPNGQSRE EWKAQAETNK ILARFKNPIP VDGICVRVGA MRCHSQALTI KLNKDVPLTD IEGLISQHNP WVKLVPNHRE VSVRELTPAA VTGTLSVPVG RLRKLNMGSQ YLGAFTVGDQ LLWGAAEPLR RMLRILLER
Validated NT Sequence
tagcgctcca gcaggatgcg caacatgcgc cgcagcggct cggcagcgcc ccacagcaac tggtcgccga cggtgaaggc gccgagatac tgggagccca tgttcaactt gcgcaggcgc ccgaccggaa cgctcagggt gccggtgacg gcggccgggg tcagctcgcg cacgcttacc tcgcggtggt tcggcaccag cttgacccag ggattgtgct ggctgatcag gccctcgatg tcggtcagcg gcacatcctt gttcaacttg atggtcagcg cctggctgtg gcaacgcatg gcgccgacgc gcacgcagat accgtcgacc gggatcgggt tcttgaagcg ggcaaggatc ttgttggtct ccgcctgggc cttccactct tcgcggctct gcccgttggg cagttccttg tcgatccagg ggatcaggct gccgcccagc ggcgcgccga aatgctcggt ggggaaggct tcgctgcgca gggtctccgc caccttgcgg tcgatgtcga ggatcgcgct ggccgggttg gccaggtcat ccgccaccga tgcatgggcg gcgcccatct gcttgagcag ttcacgcatg ttctgcgcgc cggcgccgga ggcggcctgg taggtcatgg cactcatcca ctcgaccaga ccggcgtcga acagcccgcc cagggccatc agcatcaggc tgacggtgca gttgccgccg atgtagttgc gggtgccggc gtccagagcc tggtcgatga ccttgcggtt gaccgggtcg aggacgatca ccgcgtcgtc ttccatgcgc agactggagg cggcatcgat ccagtagccc tgccagccgg cctcgcgcag cttggggaat acttcgctgg tgtagtcgcc gccctggcag gtcaggatca cgtcgagggt cttcagctcg tcgatgctgt aggcgtcctt cagcggagcg atgtccttgc ccacttccgg gccctggcca ccgacgttcg acgtggtgaa gaacaccggc tcgatcaggt cgaagtcccg ctcttccagc attcgctgca tgagtacgga acccaccatc ccgcgccaac cgatcagacc tacacgctt
Expressed Protein Sequence
MAHHHHHHMK RVGLIGWRGM VGSVLMQRML EERDFDLIEP VFFTTSNVGG QGPEVGKDIA PLKDAYSIDE LKTLDVILTC QGGDYTSEVF PKLREAGWQG YWIDAASSLR MEDDAVIVLD PVNRKVIDQA LDAGTRNYIG GNCTVSLMLM ALGGLFDAGL VEWMSAMTYQ AASGAGAQNM RELLKQMGAA HASVADDLAN PASAILDIDR KVAETLRSEA FPTEHFGAPL GGSLIPWIDK ELPNGQSREE WKAQAETNKI LARFKNPIPV DGICVRVGAM RCHSQALTIK LNKDVPLTDI EGLISQHNPW VKLVPNHREV SVRELTPAAV TGTLSVPVGR LRKLNMGSQY LGAFTVGDQL LWGAAEPLRR MLRILLER
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC GTGTAGGTCT GATCGGTTGG CGCGGGATGG TGGGTTCCGT ACTCATGCAG CGAATGCTGG AAGAGCGGGA CTTCGACCTG ATCGAGCCGG TGTTCTTCAC CACGTCGAAC GTCGGTGGCC AGGGCCCGGA AGTGGGCAAG GACATCGCTC CGCTGAAGGA CGCCTACAGC ATCGACGAGC TGAAGACCCT CGACGTGATC CTGACCTGCC AGGGCGGCGA CTACACCAGC GAAGTATTCC CCAAGCTGCG CGAGGCCGGC TGGCAGGGCT ACTGGATCGA TGCCGCCTCC AGTCTGCGCA TGGAAGACGA CGCGGTGATC GTCCTCGACC CGGTCAACCG CAAGGTCATC GACCAGGCTC TGGACGCCGG CACCCGCAAC TACATCGGCG GCAACTGCAC CGTCAGCCTG ATGCTGATGG CCCTGGGCGG GCTGTTCGAC GCCGGTCTGG TCGAGTGGAT GAGTGCCATG ACCTACCAGG CCGCCTCCGG CGCCGGCGCG CAGAACATGC GTGAACTGCT CAAGCAGATG GGCGCCGCCC ATGCATCGGT GGCGGATGAC CTGGCCAACC CGGCCAGCGC GATCCTCGAC ATCGACCGCA AGGTGGCGGA GACCCTGCGC AGCGAAGCCT TCCCCACCGA GCATTTCGGC GCGCCGCTGG GCGGCAGCCT GATCCCCTGG ATCGACAAGG AACTGCCCAA CGGGCAGAGC CGCGAAGAGT GGAAGGCCCA GGCGGAGACC AACAAGATCC TTGCCCGCTT CAAGAACCCG ATCCCGGTCG ACGGTATCTG CGTGCGCGTC GGCGCCATGC GTTGCCACAG CCAGGCGCTG ACCATCAAGT TGAACAAGGA TGTGCCGCTG ACCGACATCG AGGGCCTGAT CAGCCAGCAC AATCCCTGGG TCAAGCTGGT GCCGAACCAC CGCGAGGTAA GCGTGCGCGA GCTGACCCCG GCCGCCGTCA CCGGCACCCT GAGCGTTCCG GTCGGGCGCC TGCGCAAGTT GAACATGGGC TCCCAGTATC TCGGCGCCTT CACCGTCGGC GACCAGTTGC TGTGGGGCGC TGCCGAGCCG CTGCGGCGCA TGTTGCGCAT CCTGCTGGAG CGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT