SSGCID: Target Page

PsaeA.17880.a

Protein GrpE (HSP-70 cofactor)

CENTER ID:	PsaeA.17880.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17880.a.B1.GE38351	full length		1	186

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4988
PGAT:	pseudomonas:PA4762
RefSeq:	NP_253450.1
UniProt:	Q9HV42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADEQQTLDQ QTPEQPTGAA EDLTARVQEL EEQLAAAQDQ ALRMVADLQN VRRRAEQDVE KAHKFALEKF AGDLLAVVDT LERGLEMSDP NDEAIKPMRE GMELTLKMFD DTLRRYQVEA LNPEGEPFNP EQHQAMAMQE SASAEPGSVL KVFQKGYLLN GRLLRPAMVV VSKAPAETPP SIDEQA

NT Sequence

atggctgacg aacagcagac cctggatcaa cagacccccg agcagcccac cggcgccgcc gaggacctga ccgcccgcgt gcaggaactg gaggagcagc tggctgccgc ccaggaccag gcgctgcgca tggtcgcgga tctgcagaac gtacgccggc gtgcggagca ggatgtcgag aaggcgcaca agttcgcgct ggagaaattc gccggcgacc tgctggcggt ggtcgacacc ctcgagcgcg gcctggagat gtccgatccc aacgacgagg cgatcaagcc gatgcgcgaa gggatggaac tgaccctgaa gatgttcgac gacaccctgc gccgctacca ggtggaggcg ctgaacccgg aaggcgagcc tttcaacccc gagcagcacc aggcgatggc catgcaggaa agcgcttcgg ccgagccggg cagtgtcctc aaggtgttcc agaaaggcta cctgctcaac ggccgcctgc tgcgtccggc gatggtggta gtcagcaagg cgccggcgga aaccccgccg tccatcgacg agcaggcttg a

Details for PsaeA.17880.a.B1.GE38351

HARVESTED ON:	data unavailable
SEQUENCED ON:	7/26/2014
EXPECTED MW:	22kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA DEQQTLDQQT PEQPTGAAED LTARVQELEE QLAAAQDQAL RMVADLQNVR RRAEQDVEKA HKFALEKFAG DLLAVVDTLE RGLEMSDPND EAIKPMREGM ELTLKMFDDT LRRYQVEALN PEGEPFNPEQ HQAMAMQESA SAEPGSVLKV FQKGYLLNGR LLRPAMVVVS KAPAETPPSI DEQA

Validated NT Sequence

tcnctagaat attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatggct gacgaacagc agaccctgga tcaacagacc cccgagcagc ccaccggcgc cgccgaggac ctgaccgccc gcgtgcagga actggaggag cagctggctg ccgcccagga ccaggcgctg cgcatggtcg cggatctgca gaacgtacgc cggcgtgcgg agcaggatgt cgagaaggcg cacaagttcg cgctggagaa attcgccggc gacctgctgg cggtggtcga caccctcgag cgcggcctgg agatgtccga tcccaacgac gaggcgatca agccgatgcg cgaagggatg gaactgaccc tgaagatgtt cgacgacacc ctgcgccgct accaggtgga ggcgctgaac ccggaaggcg agcctttcaa ccccgagcag caccaggcga tggccatgca ggaaagcgct tcggccgagc cgggcagtgt cctcaaggtg ttccagaaag gctacctgct caacggccgc ctgctgcgtc cggcgatggt ggtagtcagc aaggcgccgg cggaaacccc gccgtccatc gacgagcagg cttaagtgag taagatagga tccggctgct anagaccccc ccnaannnaa nnnnnntana angcnntncc tctcggcaac cgtcctcacc gactcgttaa tccgggtgct cggagggccc cgaaatccac gacaatcatc gaaatccaga tcaaccnggt cgacttggcn ccgatacata cggctccttc ngtgacgggc ngtcttatgc gaacaanacn canttngngg tgctgatcan ntacntgtcg acaatanngg ggatggcgtg ncagaactaa cactcccnag cacgnctacc aggacacgcg ctgaagatgc tgnggggcna acttgtacna nctggaaact gcacgaanta gtaccgcnnt cntcgcagnt nntgtgaggt ntcncnagtt ccgaantcng gcnggcgncn natcnnnctc ctnannnnnn nnnncagnnn gagannatgn acnnggtatn nnttntnnnn ngaannactn nnannnannt gntnnnnnnn nnancnngat naatnctcnn nnnnnnccnc anntnggggg tgtnntgngt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg acgaacagca gaccctggat caacagaccc ccgagcagcc caccggcgcc gccgaggacc tgaccgcccg cgtgcaggaa ctggaggagc agctggctgc cgcccaggac caggcgctgc gcatggtcgc ggatctgcag aacgtacgcc ggcgtgcgga gcaggatgtc gagaaggcgc acaagttcgc gctggagaaa ttcgccggcg acctgctggc ggtggtcgac accctcgagc gcggcctgga gatgtccgat cccaacgacg aggcgatcaa gccgatgcgc gaagggatgg aactgaccct gaagatgttc gacgacaccc tgcgccgcta ccaggtggag gcgctgaacc cggaaggcga gcctttcaac cccgagcagc accaggcgat ggccatgcag gaaagcgctt cggccgagcc gggcagtgtc ctcaaggtgt tccagaaagg ctacctgctc aacggccgcc tgctgcgtcc ggcgatggtg gtagtcagca aggcgccggc ggaaaccccg ccgtccatcg acgagcaggc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat