SSGCID: Target Page

PsaeA.17879.a

tRNA(Ile)-lysidine synthase (EC 6.3.4.19) (tRNA(Ile)-2-lysyl-cytidine synthase) (tRNA(Ile)-lysidine synthetase)

CENTER ID:	PsaeA.17879.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17879.a.B1.GE38350	full length		1	442

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17879.a.B1.PW37592	full length		1	442

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3807
PGAT:	pseudomonas:PA3638
RefSeq:	NP_252328.1
UniProt:	Q9HXZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAVPLESRL LQALAPWREA GGWCVAFSGG LDSTVLLHLL AQLARSEALP ALSALHVHHG LQAAADGWPA HCQAVCRSLG IPLRVERVQV AVGGSIEQAA RDARYRAFQA NLGEGQVLLT AQHLDDQAET LLFRLLRGAG LRGLAAMPAS RPLGGGRLCR PLLGVSRAEL EAYAQAHRLD WVEDPSNQDP RFSRNYLRRE IMPRLASHWP QAVAGMARCA AHLREAEDLL AELAAIDLRA CRQPSELDWP DWLDLPRIAL EPLRRLSAAR QRNLLRAWLG QLTRLPDSDH WAGWESLRDA GDDADPIWRL ESGELRRGAG RIWWLPADWR AAAGPFVWER PAHPLLLPGN GRLALLGEVP AGPLRVDYRR GGEVLALAGR GRRDLKRLLN EAGVPSFLRG RLPLLFRADE LLAVANIPGL DSPREGGWRL SWSPPTNDPG LS

NT Sequence

atgccggcgg tgccgctcga atcccgtctg ttacaggccc tggcgccctg gcgcgaagcg gggggctggt gcgtggcgtt ttccggcggg ctggattcca cggtgttgct gcatctgctc gcccaactgg cccggagcga agccttgccg gcgctgtcgg cgctgcatgt ccaccatggc ttgcaggctg cggcggacgg ttggccggcg cactgccagg cggtctgccg gtcgctgggt atcccgctgc gggtcgagcg ggtgcaggtc gccgttggcg gcagcatcga gcaggcggcg cgcgacgccc gctatcgcgc attccaggcg aacctgggcg agggacaggt gctgctcacc gcgcagcacc tcgacgacca ggccgagacc ttgctgttcc gcctgttgcg tggagcgggc ctgcgcggcc tggcggcgat gccggcgagc cggccgctgg gcggtggccg cctgtgccga cccttgctcg gggtttcccg cgccgaactg gaagcctatg cgcaggcgca ccggctggac tgggtggagg atccttccaa ccaggatccg cgcttctcgc gcaactacct gcgccgggag atcatgccgc gtctcgcctc ccactggccg caggccgtcg ccggcatggc gcgctgcgcg gcgcatctgc gcgaggccga agacctgctg gctgaactgg ccgccatcga cctccgggcc tgccgccaac cctcggaact ggattggccg gactggctgg atctgccccg gatcgccctg gagcctctgc gccgcctcag cgctgcgcgc cagcgcaacc tgctgcgggc ctggctggga cagctgacgc ggttgccgga cagtgaccac tgggccggtt gggagtcgtt gcgcgacgcc ggcgacgatg ccgacccgat ctggcgcctg gagagcggcg agctgcgtcg cggcgcgggg cggatctggt ggttgccggc ggactggcgg gcggcggcgg gaccgttcgt ctgggagcgt cccgctcatc cattgcttct tcccggcaat ggtcggctgg cgttgctcgg cgaggttccg gcgggaccgt tgcgcgtcga ctaccggcgg ggtggggagg tgctggccct ggccgggcgc ggtcgccgcg acctcaagcg attgctcaac gaagcgggcg taccgagctt cctgcgcggt cgtttgccgc tgctcttccg agccgacgaa ctgctcgcgg tggccaacat tcctggcctg gacagtcccc gcgagggggg ctggcgactg tcctggagcc cgccgacgaa cgacccgggt ttgagctag

Details for PsaeA.17879.a.B1.GE38350

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	50kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	84

Validated AA Sequence

MAHHHHHHMP AVPLESRLLQ ALAPWREAGG WCVAFSGGLD STVLLHLLAQ LARSEALPAL SALHVHHGLQ AAADGWPAHC QAVCRSLGIP LRVERVQVAV GGSIEQAARD ARYRAFQANL GEGQVLLTAQ HLDDQAETLL FRLLRGAGLR GLAAMPASRP LGGGRLCRPL LGVSRAELEA YAQAHRLDWV EDPSNQDPRF SRNYLRREIM PRLASHWPQA VAGMARCAAH LREAEDLLAE LAAIDLRACR QPSELDWPDW LDLPRIALEP LRRLSAARQR NLLRAWLGQL TRLPDSDHWA GWESLRDAGD DADPIWRLXS GELRRXRGGS GGXRXLXARR DRSSWXVPLI XXFFRXXXXX XLGXXXXXXX XXXXXXXXXX P

Validated NT Sequence

cnnnnnnnnt cnntanatat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgg cggtgccgct cgaatcccgt ctgttacagg ccctggcgcc ctggcgcgaa gcggggggct ggtgcgtggc gttttccggc gggctggatt ccacggtgtt gctgcatctg ctcgcccaac tggcccggag cgaagccttg ccggcgctgt cggcgctgca tgtccaccat ggcttgcagg ctgcggcgga cggttggccg gcgcactgcc aggcggtctg ccggtcgctg ggtatcccgc tgcgggtcga gcgggtgcag gtcgccgttg gcggcagcat cgagcaggcg gcgcgcgacg cccgctatcg cgcattccag gcgaacctgg gcgagggaca ggtgctgctc accgcgcagc acctcgacga ccaggccgag accttgctgt tccgcctgtt gcgtggagcg ggcctgcgcg gcctggcggc gatgccggcg agccggccgc tgggcggtgg ccgcctgtgc cgacccttgc tcggggtttc ccgcgccgaa ctggaagcct atgcgcaggc gcaccggctg gactgggtgg aggatccttc caaccaggat ccgcgcttct cgcgcaacta cctgcgccgg gagatcatgc cgcgtctcgc ctcccactgg ccgcaggccg tcgccggcat ggcgcgctgc gcggcgcatc tgcgcgaggc cgaagacctg ctggctgaac tggccgccat cgacctccgg gcctgccgcc aaccctcgga actggattgg ccggactggc tggatctgcc ccggatcgcc ctggagcctc tgcgccgcct cagcgctgcg cgccagcgca acctgctgcg ggcctggctg ggncagctga cgcggttgcc ggacagtgac cactgggccg gttgggagtc gttgcgcgac gccggcgacg atgccgaccc gatctggcgc ctgganagcg gcgagctgcg tcgcgngcgg ggcggatctg gtggtngccg gcnnctnngg gcgcgncggg accgttcgtc ntggnncgtc ccgctcatcc ntngcttctt ccgnnatnnn gnntnnnnnn ncctcggnng cncnntnnan nncnnngnnn nnnnnnnntn nnngnnngnn nncgnntncc cncna

Expected Protein Sequence

MAHHHHHHMP AVPLESRLLQ ALAPWREAGG WCVAFSGGLD STVLLHLLAQ LARSEALPAL SALHVHHGLQ AAADGWPAHC QAVCRSLGIP LRVERVQVAV GGSIEQAARD ARYRAFQANL GEGQVLLTAQ HLDDQAETLL FRLLRGAGLR GLAAMPASRP LGGGRLCRPL LGVSRAELEA YAQAHRLDWV EDPSNQDPRF SRNYLRREIM PRLASHWPQA VAGMARCAAH LREAEDLLAE LAAIDLRACR QPSELDWPDW LDLPRIALEP LRRLSAARQR NLLRAWLGQL TRLPDSDHWA GWESLRDAGD DADPIWRLES GELRRGAGRI WWLPADWRAA AGPFVWERPA HPLLLPGNGR LALLGEVPAG PLRVDYRRGG EVLALAGRGR RDLKRLLNEA GVPSFLRGRL PLLFRADELL AVANIPGLDS PREGGWRLSW SPPTNDPGLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgg cggtgccgct cgaatcccgt ctgttacagg ccctggcgcc ctggcgcgaa gcggggggct ggtgcgtggc gttttccggc gggctggatt ccacggtgtt gctgcatctg ctcgcccaac tggcccggag cgaagccttg ccggcgctgt cggcgctgca tgtccaccat ggcttgcagg ctgcggcgga cggttggccg gcgcactgcc aggcggtctg ccggtcgctg ggtatcccgc tgcgggtcga gcgggtgcag gtcgccgttg gcggcagcat cgagcaggcg gcgcgcgacg cccgctatcg cgcattccag gcgaacctgg gcgagggaca ggtgctgctc accgcgcagc acctcgacga ccaggccgag accttgctgt tccgcctgtt gcgtggagcg ggcctgcgcg gcctggcggc gatgccggcg agccggccgc tgggcggtgg ccgcctgtgc cgacccttgc tcggggtttc ccgcgccgaa ctggaagcct atgcgcaggc gcaccggctg gactgggtgg aggatccttc caaccaggat ccgcgcttct cgcgcaacta cctgcgccgg gagatcatgc cgcgtctcgc ctcccactgg ccgcaggccg tcgccggcat ggcgcgctgc gcggcgcatc tgcgcgaggc cgaagacctg ctggctgaac tggccgccat cgacctccgg gcctgccgcc aaccctcgga actggattgg ccggactggc tggatctgcc ccggatcgcc ctggagcctc tgcgccgcct cagcgctgcg cgccagcgca acctgctgcg ggcctggctg ggacagctga cgcggttgcc ggacagtgac cactgggccg gttgggagtc gttgcgcgac gccggcgacg atgccgaccc gatctggcgc ctggagagcg gcgagctgcg tcgcggcgcg gggcggatct ggtggttgcc ggcggactgg cgggcggcgg cgggaccgtt cgtctgggag cgtcccgctc atccattgct tcttcccggc aatggtcggc tggcgttgct cggcgaggtt ccggcgggac cgttgcgcgt cgactaccgg cggggtgggg aggtgctggc cctggccggg cgcggtcgcc gcgacctcaa gcgattgctc aacgaagcgg gcgtaccgag cttcctgcgc ggtcgtttgc cgctgctctt ccgagccgac gaactgctcg cggtggccaa cattcctggc ctggacagtc cccgcgaggg gggctggcga ctgtcctgga gcccgccgac gaacgacccg ggtttgagct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for PsaeA.17879.a.B1.PW37592

PURIFICATION DATe:	11/10/2014
CONCENTRATION:	27mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	87
PERCENT COVERAGE:	84

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMP AVPLESRLLQ ALAPWREAGG WCVAFSGGLD STVLLHLLAQ LARSEALPAL SALHVHHGLQ AAADGWPAHC QAVCRSLGIP LRVERVQVAV GGSIEQAARD ARYRAFQANL GEGQVLLTAQ HLDDQAETLL FRLLRGAGLR GLAAMPASRP LGGGRLCRPL LGVSRAELEA YAQAHRLDWV EDPSNQDPRF SRNYLRREIM PRLASHWPQA VAGMARCAAH LREAEDLLAE LAAIDLRACR QPSELDWPDW LDLPRIALEP LRRLSAARQR NLLRAWLGQL TRLPDSDHWA GWESLRDAGD DADPIWRLES GELRRGAGRI WWLPADWRAA AGPFVWERPA HPLLLPGNGR LALLGEVPAG PLRVDYRRGG EVLALAGRGR RDLKRLLNEA GVPSFLRGRL PLLFRADELL AVANIPGLDS PREGGWRLSW SPPTNDPGLS

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGG CGGTGCCGCT CGAATCCCGT CTGTTACAGG CCCTGGCGCC CTGGCGCGAA GCGGGGGGCT GGTGCGTGGC GTTTTCCGGC GGGCTGGATT CCACGGTGTT GCTGCATCTG CTCGCCCAAC TGGCCCGGAG CGAAGCCTTG CCGGCGCTGT CGGCGCTGCA TGTCCACCAT GGCTTGCAGG CTGCGGCGGA CGGTTGGCCG GCGCACTGCC AGGCGGTCTG CCGGTCGCTG GGTATCCCGC TGCGGGTCGA GCGGGTGCAG GTCGCCGTTG GCGGCAGCAT CGAGCAGGCG GCGCGCGACG CCCGCTATCG CGCATTCCAG GCGAACCTGG GCGAGGGACA GGTGCTGCTC ACCGCGCAGC ACCTCGACGA CCAGGCCGAG ACCTTGCTGT TCCGCCTGTT GCGTGGAGCG GGCCTGCGCG GCCTGGCGGC GATGCCGGCG AGCCGGCCGC TGGGCGGTGG CCGCCTGTGC CGACCCTTGC TCGGGGTTTC CCGCGCCGAA CTGGAAGCCT ATGCGCAGGC GCACCGGCTG GACTGGGTGG AGGATCCTTC CAACCAGGAT CCGCGCTTCT CGCGCAACTA CCTGCGCCGG GAGATCATGC CGCGTCTCGC CTCCCACTGG CCGCAGGCCG TCGCCGGCAT GGCGCGCTGC GCGGCGCATC TGCGCGAGGC CGAAGACCTG CTGGCTGAAC TGGCCGCCAT CGACCTCCGG GCCTGCCGCC AACCCTCGGA ACTGGATTGG CCGGACTGGC TGGATCTGCC CCGGATCGCC CTGGAGCCTC TGCGCCGCCT CAGCGCTGCG CGCCAGCGCA ACCTGCTGCG GGCCTGGCTG GGACAGCTGA CGCGGTTGCC GGACAGTGAC CACTGGGCCG GTTGGGAGTC GTTGCGCGAC GCCGGCGACG ATGCCGACCC GATCTGGCGC CTGGAGAGCG GCGAGCTGCG TCGCGGCGCG GGGCGGATCT GGTGGTTGCC GGCGGACTGG CGGGCGGCGG CGGGACCGTT CGTCTGGGAG CGTCCCGCTC ATCCATTGCT TCTTCCCGGC AATGGTCGGC TGGCGTTGCT CGGCGAGGTT CCGGCGGGAC CGTTGCGCGT CGACTACCGG CGGGGTGGGG AGGTGCTGGC CCTGGCCGGG CGCGGTCGCC GCGACCTCAA GCGATTGCTC AACGAAGCGG GCGTACCGAG CTTCCTGCGC GGTCGTTTGC CGCTGCTCTT CCGAGCCGAC GAACTGCTCG CGGTGGCCAA CATTCCTGGC CTGGACAGTC CCCGCGAGGG GGGCTGGCGA CTGTCCTGGA GCCCGCCGAC GAACGACCCG GGTTTGAGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT