SSGCID: Target Page

PsaeA.17874.a

Elongation factor Ts (EF-Ts)

CENTER ID:	PsaeA.17874.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17874.a.B1.GE38348	full length		1	289

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17874.a.B1.PS02327	full length		1	289

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3824
PGAT:	pseudomonas:PA3655
RefSeq:	NP_252345.1
UniProt:	O82851

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEITAAMVK ELRERTGLGM MECKKALTAA GGDIEKAIDD MRAAGAIKAA KKAGNIAAEG SIAVKIAADN KAAVIIEVNS QTDFLALQDD FKGFVAESLE KAFNEKLTDA APLVEAREEA RLALVAKTGE NVNIRRLTRV EGDVVGAYLH GHRIGVVVNL KGGNPELAKD IAMHVAASNP QFLSASEVSE EAIAKEKEIF LALNADKIAG KPENIVENMV KGRISKFLAE ASLVEQPFVK NPEVKVGDLA KQAGAEIVSF VRYEVGEGIE KAEVDFAAEV AAQVAATKQ

NT Sequence

atggcagaaa ttactgcagc catggttaaa gaactgcgcg agcgtaccgg cttgggcatg atggagtgca agaaggcgct gaccgccgct ggcggcgaca tcgagaaggc catcgacgac atgcgcgctg ccggtgcgat caaggctgcc aagaaggccg gcaacattgc cgccgaaggc tcgatcgccg tcaagatcgc tgccgacaac aaggccgccg tgatcatcga agtcaactcc cagaccgact tcctggccct ccaggacgac ttcaagggct tcgtcgccga gagcctggaa aaggccttca acgagaagct gaccgacgcc gctccgctgg tcgaagcacg tgaggaagct cgtctggccc tggtcgccaa gaccggcgag aacgtcaaca tccgccgcct gacccgcgtc gagggcgacg tggtcggcgc ctacctgcac ggccaccgca tcggtgtcgt ggtcaacctg aaaggcggca acccggaact ggccaaggac atcgccatgc atgtcgccgc cagcaacccg cagttcctga gcgcttcgga agtttccgaa gaagcgatcg cgaaagagaa ggaaatcttc ctggcgctga acgccgacaa gatcgccggc aagccggaga acatcgtcga gaacatggtc aagggccgca tcagcaagtt cctcgccgaa gcgagcctgg tcgagcagcc tttcgtgaag aacccggaag tgaaggtcgg cgacctggcc aagcaggccg gtgccgaaat cgtttccttc gttcgttacg aagtgggtga aggcatcgag aaggccgaag tggacttcgc tgccgaagtt gccgctcaag tagccgccac caagcagtaa

Details for PsaeA.17874.a.B1.GE38348

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	93
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA EITAAMVKEL RERTGLGMME CKKALTAAGG DIEKAIDDMR AAGAIKAAKK AGNIAAEGSI AVKIAADNKA AVIIEVNSQT DFLALQDDFK GFVAESLEKA FNEKLTDAAP LVEAREEARL ALVAKTGENV NIRRLTRVEG DVVGAYLHGH RIGVVVNLKG GNPELAKDIA MHVAASNPQF LSASEVSEEA IAKEKEIFLA LNADKIAGKP ENIVENMVKG RISKFLAEAS LVEQPFVKNP EVKVGDLAKX PVPKSFPSFV TKWVKASRKA EVDFAAEVAA QVAATKQ

Validated NT Sequence

tnnatatttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atggcagaaa ttactgcagc catggttaaa gaactgcgcg agcgtaccgg cttgggcatg atggagtgca agaaggcgct gaccgccgct ggcggcgaca tcgagaaggc catcgacgac atgcgcgctg ccggtgcgat caaggctgcc aagaaggccg gcaacattgc cgccgaaggc tcgatcgccg tcaagatcgc tgccgacaac aaggccgccg tgatcatcga agtcaactcc cagaccgact tcctggccct ccaggacgac ttcaagggct tcgtcgccga gagcctggaa aaggccttca acgagaagct gaccgacgcc gctccgctgg tcgaagcacg tgaggaagct cgtctggccc tggtcgccaa gaccggcgag aacgtcaaca tccgccgcct gacccgcgtc gagggcgacg tggtcggcgc ctacctgcac ggccaccgca tcggtgtcgt ggtcaacctg aaaggcggca acccggaact ggccaaggac atcgccatgc atgtcgccgc cagcaacccg cagttcctga gcgcttcgga agtttccgaa gaagcgatcg cgaaagagaa ggaaatcttc ctggcgctga acgccgacaa gatcgccggc aagccggaga acatcgtcga gaacatggtc aagggccgca tcagcaagtt cctcgccgaa gcgagcctgg tcgagcagcc tttcgtgaag aacccggaag tgaaggtcgg cgacctggcc aagcngccgg tgccgaaatc gtttccttcg ttcgttacga agtgggtgaa ggcatcgaga aaggccgaag tggacttcgc tgccgaagtt gccgctcaag tagccgccac caagcagtaa gtgagtaaga taggatccnn tgctannann ccccaagaga gnnccnnnan nnggannnnn tctgacnccn gcngcataga tactgnngca nncntatgnn ngcgcactgt actnngnncc antcgctncn ngnntccgnn nnnntgccca nnccnnnnnn nntnnntnca aacatnggan tgnnnnannc nnntannngn nnngnngang nnaannnnnt nntnnnnnng g

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag aaattactgc agccatggtt aaagaactgc gcgagcgtac cggcttgggc atgatggagt gcaagaaggc gctgaccgcc gctggcggcg acatcgagaa ggccatcgac gacatgcgcg ctgccggtgc gatcaaggct gccaagaagg ccggcaacat tgccgccgaa ggctcgatcg ccgtcaagat cgctgccgac aacaaggccg ccgtgatcat cgaagtcaac tcccagaccg acttcctggc cctccaggac gacttcaagg gcttcgtcgc cgagagcctg gaaaaggcct tcaacgagaa gctgaccgac gccgctccgc tggtcgaagc acgtgaggaa gctcgtctgg ccctggtcgc caagaccggc gagaacgtca acatccgccg cctgacccgc gtcgagggcg acgtggtcgg cgcctacctg cacggccacc gcatcggtgt cgtggtcaac ctgaaaggcg gcaacccgga actggccaag gacatcgcca tgcatgtcgc cgccagcaac ccgcagttcc tgagcgcttc ggaagtttcc gaagaagcga tcgcgaaaga gaaggaaatc ttcctggcgc tgaacgccga caagatcgcc ggcaagccgg agaacatcgt cgagaacatg gtcaagggcc gcatcagcaa gttcctcgcc gaagcgagcc tggtcgagca gcctttcgtg aagaacccgg aagtgaaggt cggcgacctg gccaagcagg ccggtgccga aatcgtttcc ttcgttcgtt acgaagtggg tgaaggcatc gagaaggccg aagtggactt cgctgccgaa gttgccgctc aagtagccgc caccaagcag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for PsaeA.17874.a.B1.PS02327

PURIFICATION DATe:	2/11/2015
CONCENTRATION:	55.85mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	93
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG AAATTACTGC AGCCATGGTT AAAGAACTGC GCGAGCGTAC CGGCTTGGGC ATGATGGAGT GCAAGAAGGC GCTGACCGCC GCTGGCGGCG ACATCGAGAA GGCCATCGAC GACATGCGCG CTGCCGGTGC GATCAAGGCT GCCAAGAAGG CCGGCAACAT TGCCGCCGAA GGCTCGATCG CCGTCAAGAT CGCTGCCGAC AACAAGGCCG CCGTGATCAT CGAAGTCAAC TCCCAGACCG ACTTCCTGGC CCTCCAGGAC GACTTCAAGG GCTTCGTCGC CGAGAGCCTG GAAAAGGCCT TCAACGAGAA GCTGACCGAC GCCGCTCCGC TGGTCGAAGC ACGTGAGGAA GCTCGTCTGG CCCTGGTCGC CAAGACCGGC GAGAACGTCA ACATCCGCCG CCTGACCCGC GTCGAGGGCG ACGTGGTCGG CGCCTACCTG CACGGCCACC GCATCGGTGT CGTGGTCAAC CTGAAAGGCG GCAACCCGGA ACTGGCCAAG GACATCGCCA TGCATGTCGC CGCCAGCAAC CCGCAGTTCC TGAGCGCTTC GGAAGTTTCC GAAGAAGCGA TCGCGAAAGA GAAGGAAATC TTCCTGGCGC TGAACGCCGA CAAGATCGCC GGCAAGCCGG AGAACATCGT CGAGAACATG GTCAAGGGCC GCATCAGCAA GTTCCTCGCC GAAGCGAGCC TGGTCGAGCA GCCTTTCGTG AAGAACCCGG AAGTGAAGGT CGGCGACCTG GCCAAGCAGG CCGGTGCCGA AATCGTTTCC TTCGTTCGTT ACGAAGTGGG TGAAGGCATC GAGAAGGCCG AAGTGGACTT CGCTGCCGAA GTTGCCGCTC AAGTAGCCGC CACCAAGCAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT