PsaeA.17873.a

Biotin carboxyl carrier protein of acetyl-CoA carboxylase (BCCP)

CENTER ID: PsaeA.17873.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17873.a.B1.GE38347 full length 1 156
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5079
PGAT: pseudomonas:PA4847
RefSeq: NP_253534.1
UniProt: P37799

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDIRKVKKLI ELLEESGIDE LEIREGEESV RISRHSKTAA QPVYAQAPAF AAPVAAPAPA AAAPAAAAAE SAPAAPKLNG NVVRSPMVGT FYRAASPTSA NFVEVGQSVK KGDILCIVEA MKMMNHIEAE VSGTIESILV ENGQPVEFDQ PLFTIV
NT Sequence
atggacattc gtaaagtcaa gaaactgatc gagctgctgg aagagtccgg tatcgacgag ctggaaatcc gcgaaggcga agagtcggta cgcatcagcc gccacagcaa gaccgccgcc cagccggtgt acgcacaggc tccggccttc gccgctccgg tcgccgcgcc ggcgccggca gccgccgctc cggccgccgc tgccgcggaa agcgccccgg ccgctccgaa gctgaacggc aacgtggttc gctcgccgat ggtcggcacc ttctaccgcg ccgcctcgcc gacctcggcc aacttcgtcg aagtcggcca gagcgtgaag aaaggcgaca tcctgtgcat cgtcgaagcc atgaagatga tgaaccacat cgaagccgaa gttagcggca ccatcgagtc gatcctggtg gagaacggcc agccggttga gttcgaccag ccgctgttca ccatcgtcta a
Details for PsaeA.17873.a.B1.GE38347
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 17kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD IRKVKKLIEL LEESGIDELE IREGEESVRI SRHSKTAAQP VYAQAPAFAA PVAAPAPAAA APAAAAAESA PAAPKLNGNV VRSPMVGTFY RAASPTSANF VEVGQSVKKG DILCIVEAMK MMNHIEAEVS GTIESILVEN GQPVEFDQPL FTIV
Validated NT Sequence
annataattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggacatt cgtaaagtca agaaactgat cgagctgctg gaagagtccg gtatcgacga gctggaaatc cgcgaaggcg aagagtcggt acgcatcagc cgccacagca agaccgccgc ccagccggtg tacgcacagg ctccggcctt cgccgctccg gtcgccgcgc cggcgccggc agccgccgct ccggccgccg ctgccgcgga aagcgccccg gccgctccga agctgaacgg caacgtggtt cgctcgccga tggtcggcac cttctaccgc gccgcctcgc cgacctcggc caacttcgtc gaagtcggcc agagcgtgaa gaaaggcgac atcctgtgca tcgtcgaagc catgaagatg atgaaccaca tcgaagccga agttagcggc accatcgagt cgatcctggt ggagaacggc cagccggttg agttcgacca gccgctgttc accatcgtct aagtgagtaa gataggatcc ggctgctaac aagccnccga aagannntaa gaaacgnnat cctggtttcg ggaatccggt atctgcgctg ctgctatcca gacctgtgcg gagtcgaatc cactcatccn ttgccgaaac gggactgcgc aaagtgccct ctaacctgct cgatggcaca acaaccaatc ccttggcgaa tcgggggctg gccccgggtc cgactgggcc gaccccggat acgcactggg aatccgccaa cggccnttcc gtgcntgcgc ntgacatact ccacctccca ctggccgncc aacccgtnnt cncnctcccn tcgncagtcg cnatgcctct cngcnangnn ctgccttcna ccctggagta ccgtnanccc tgcntccccg cntcctgtcc nggaaacggc gnnnnnnnnc nnnnnnnntg nntggtacta acnngctnna tcccctgtnc nccnannact ctntcncacn tgttttgcnc nnntggcncn attnctgcct tnnctntntc tatccncctc gnnccncgga cananctcgn ncntgtctac ctggnanctc ttgntccggc ctttngtttc aggtattngg gatntnttga nccnttnanc
Expected Protein Sequence
MAHHHHHHMD IRKVKKLIEL LEESGIDELE IREGEESVRI SRHSKTAAQP VYAQAPAFAA PVAAPAPAAA APAAAAAESA PAAPKLNGNV VRSPMVGTFY RAASPTSANF VEVGQSVKKG DILCIVEAMK MMNHIEAEVS GTIESILVEN GQPVEFDQPL FTIV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca ttcgtaaagt caagaaactg atcgagctgc tggaagagtc cggtatcgac gagctggaaa tccgcgaagg cgaagagtcg gtacgcatca gccgccacag caagaccgcc gcccagccgg tgtacgcaca ggctccggcc ttcgccgctc cggtcgccgc gccggcgccg gcagccgccg ctccggccgc cgctgccgcg gaaagcgccc cggccgctcc gaagctgaac ggcaacgtgg ttcgctcgcc gatggtcggc accttctacc gcgccgcctc gccgacctcg gccaacttcg tcgaagtcgg ccagagcgtg aagaaaggcg acatcctgtg catcgtcgaa gccatgaaga tgatgaacca catcgaagcc gaagttagcg gcaccatcga gtcgatcctg gtggagaacg gccagccggt tgagttcgac cagccgctgt tcaccatcgt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat