PsaeA.17870.a

50S ribosomal protein L6

CENTER ID: PsaeA.17870.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17870.a.B1.GE38345 full length 1 177
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4449
PGAT: pseudomonas:PA4248
RefSeq: NP_252938.1
UniProt: Q9HWF0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRVAKNPVK LPAGVEIKLA GQQLSIKGAK GALELKVHPS VEVIQDSGEL RFAARNGDQQ TRAMAGTTRA LVNNMVVGVS QGFERKLQLV GVGYKAQAKG QVLSLSLGFS HPVDYELPAG IVAETPSQTD ILIKGIDKQL VGQVAAEIRD FRPPEPYKGK GVRYADEVVR RKEAKKK
NT Sequence
atgtctcgcg ttgctaagaa ccccgtcaag ctgcctgccg gcgttgaaat caaactggct ggtcagcaac tgtccatcaa gggcgccaaa ggtgctctgg agctcaaggt ccatccttcg gtcgaagtga tccaggacag cggtgagctg cgttttgctg cgcgtaacgg cgaccagcag actcgcgcca tggctggtac cacccgtgct ctggtgaaca acatggtagt cggcgtcagc cagggcttcg agcgcaagct ccagctggtc ggcgttggtt acaaagcgca ggccaagggc caagtgctgt ccctgtccct cggcttctcc catccggttg attacgaact gccggccggc atcgtcgctg agacccccag ccagaccgat atcctgatca agggtatcga caagcagctg gtcggtcagg ttgctgccga aattcgcgac ttccgtccgc cggagcctta caaaggcaag ggcgtgcgtt acgccgacga agtcgtccgt cgtaaagaag ccaagaagaa gtag
Details for PsaeA.17870.a.B1.GE38345
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 20kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS RVAKNPVKLP AGVEIKLAGQ QLSIKGAKGA LELKVHPSVE VIQDSGELRF AARNGDQQTR AMAGTTRALV NNMVVGVSQG FERKLQLVGV GYKAQTKGQV LSLSLGFSHP VDYELPAGIV AETPSQTDIL IKGIDKQLVG QVAAEIRDFR PPEPYKGKGV RYADEVVRRK EAKKK
Validated NT Sequence
ttccctctan annttttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg tctcgcgttg ctaagaaccc cgtcaagctg cctgccggcg ttgaaatcaa actggctggt cagcaactgt ccatcaaggg cgccaaaggt gctctggagc tcaaggtcca tccttcggtc gaagtgatcc aggacagcgg tgagctgcgt tttgctgcgc gtaacggcga ccagcagact cgcgccatgg ctggtaccac ccgtgctctg gtgaacaaca tggtagtcgg cgtcagccag ggcttcgagc gcaagctcca gctggtcggc gttggttaca aagcgcagac caagggccaa gtgctgtccc tgtccctcgg cttctcccat ccggttgatt acgaactgcc ggccggcatc gtcgctgaga cccccagcca gaccgatatc ctgatcaagg gtatcgacaa gcagctggtc ggtcaggttg ctgccgaaat tcgcgacttc cgtccgccgg agccttacaa aggcaagggc gtgcgttacg ccgacgaagt cgtccgtcgt aaagaagcca agaagaagta agtgagtaag ataggatccg gctgctaaca ggnccngaan ganttnccgg ttcccgaacg aacccctgcc cacctacgcg caccccctga acaaggtctg acactcccct cnactgaagc catcccgccg aacaaactga aaggccaggt acncgcctct cactcacttg ggccggtctg caaacgctcc ctcgcccatc ccgcagctga cagtccnccc cgcctccnng gggcaagaca acggtggggc ccanaaccat tnttccatcc cttgccgcgc ccgcctgnta gatgactggc tgcgaaggcc cccctgtncc tgacccgttc cgnatctccc ttttttggaa ncc
Expected Protein Sequence
MAHHHHHHMS RVAKNPVKLP AGVEIKLAGQ QLSIKGAKGA LELKVHPSVE VIQDSGELRF AARNGDQQTR AMAGTTRALV NNMVVGVSQG FERKLQLVGV GYKAQAKGQV LSLSLGFSHP VDYELPAGIV AETPSQTDIL IKGIDKQLVG QVAAEIRDFR PPEPYKGKGV RYADEVVRRK EAKKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc gcgttgctaa gaaccccgtc aagctgcctg ccggcgttga aatcaaactg gctggtcagc aactgtccat caagggcgcc aaaggtgctc tggagctcaa ggtccatcct tcggtcgaag tgatccagga cagcggtgag ctgcgttttg ctgcgcgtaa cggcgaccag cagactcgcg ccatggctgg taccacccgt gctctggtga acaacatggt agtcggcgtc agccagggct tcgagcgcaa gctccagctg gtcggcgttg gttacaaagc gcaggccaag ggccaagtgc tgtccctgtc cctcggcttc tcccatccgg ttgattacga actgccggcc ggcatcgtcg ctgagacccc cagccagacc gatatcctga tcaagggtat cgacaagcag ctggtcggtc aggttgctgc cgaaattcgc gacttccgtc cgccggagcc ttacaaaggc aagggcgtgc gttacgccga cgaagtcgtc cgtcgtaaag aagccaagaa gaagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat