PsaeA.17869.b

Probable iron-binding protein IscA

CENTER ID: PsaeA.17869.b
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17869.b.B1.GE38344 full length 1 107
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3991
PGAT: pseudomonas:PA3812
RefSeq: NP_252501.1
UniProt: Q9HXJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAISMTEAAA KHVQRSLEGR GKGEGIRLGV RTTGCSGLAY VLEFVDELAA EDLVFESHGV KVIIDPKSLV YLDGTELDFT REGLNEGFKF NNPNVRGECG CGESFNV
NT Sequence
atggccatca gcatgaccga agccgccgcc aaacacgtgc agcgctccct cgaggggcgc ggcaagggcg agggtatccg tcttggcgtg cgcaccactg gctgttccgg gcttgcctat gtcctcgaat tcgtcgatga actggcggcc gaggacttgg tcttcgagag ccatggcgtg aaggtcatca tcgacccgaa aagcctggtc tatctcgatg gcaccgagct ggacttcact cgcgaagggc tcaacgaagg cttcaagttc aacaatccca acgttcgtgg cgaatgcggc tgcggcgaaa gcttcaacgt ctga
Details for PsaeA.17869.b.B1.GE38344
HARVESTED ON: data unavailable
SEQUENCED ON: 7/26/2014
EXPECTED MW: 13kDa
OBSERVED MW: 10kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ISMTEAAAKH VQRSLEGRGK GEGIRLGVRT TGCSGLAYVL EFVDELAAED LVFESHGVKV IIDPKSLVYL DGTELDFTRE GLNEGFKFNN PNVRGECGCG ESFNV
Validated NT Sequence
aanttnntct agantatttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atggccatca gcatgaccga agccgccgcc aaacacgtgc agcgctccct cgaggggcgc ggcaagggcg agggtatccg tcttggcgtg cgcaccactg gctgttccgg gcttgcctat gtcctcgaat tcgtcgatga actggcggcc gaggacttgg tcttcgagag ccatggcgtg aaggtcatca tcgacccgaa aagcctggtc tatctcgatg gcaccgagct ggacttcact cgcgaagggc tcaacgaagg cttcaagttc aacaatccca acgttcgtgg cgaatgcggc tgcggcgaaa gcttcaacgt ctaagtgagt aagataggat ccggctgcta acaagccncc naaagancca gcagcngngn gtcctcganc cgtggggcgc cnnctcgact tcggtttcat ccaacgcgtg acagatggcc tgacgttcct ccgggtcaag ggagggatga gcctggacag gctcaccgcg ccgnccaacg ctgaagccaa gtggatcacc aggccctgcg cgcgttcgcc atggcagcgc tcgtgcgctc gctcgggcga ggacttgctc cggcgccgac gcgttgctgt gcggacgatg ggcgactcat cgaacgcgcc cacgaactgg gcctgaacgg gcacgttacc tcgctcgccg cgactcaaca gccgttgctg atgcgatacg acccggctnn nncgcatacc ccctggagac nncnttctac caggccngac cgggcgctgg tggagancnt gtccaccgcc tccnnncgcg aattcatnca ggacgcctcc nacnaactgt tngcgctgcc ttcgcctagg gncaantcng attttcacta nctgctcctg gaacatgacg ntncgcgacn nctacgaatc gnttgttgga ggcccgtccg tcttcntggt tcggcnccnt ttgcccggct nngnncttgg tncatnagna nncnnntngt tctgatnnnn cnnagatann ctcgggtnct ngcccnnttc tttttntnnn natngtcg
Expected Protein Sequence
MAHHHHHHMA ISMTEAAAKH VQRSLEGRGK GEGIRLGVRT TGCSGLAYVL EFVDELAAED LVFESHGVKV IIDPKSLVYL DGTELDFTRE GLNEGFKFNN PNVRGECGCG ESFNV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca tcagcatgac cgaagccgcc gccaaacacg tgcagcgctc cctcgagggg cgcggcaagg gcgagggtat ccgtcttggc gtgcgcacca ctggctgttc cgggcttgcc tatgtcctcg aattcgtcga tgaactggcg gccgaggact tggtcttcga gagccatggc gtgaaggtca tcatcgaccc gaaaagcctg gtctatctcg atggcaccga gctggacttc actcgcgaag ggctcaacga aggcttcaag ttcaacaatc ccaacgttcg tggcgaatgc ggctgcggcg aaagcttcaa cgtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat