SSGCID: Target Page

PsaeA.17867.a

Electron transfer flavoprotein subunit alpha (Alpha-ETF) (Electron transfer flavoprotein large subunit) (ETFLS)

CENTER ID:	PsaeA.17867.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17867.a.B1.GE38342	full length		1	309

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3097
PGAT:	pseudomonas:PA2951
RefSeq:	NP_251641.1
UniProt:	Q9HZP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAILVIAEHN NAALAAATLN TVAAAKAIGG DIHVLVAGQN VAAVAEAAAK VEGVSKVLVA DNAAYAHQLP ENVAPLIAEL GKNYSHVLAP ATTNGKNFLP RVAALLDVDQ ISEIVEVVSP DTFKRPIYAG NAIATVQSSA AVKVITVRTT GFDAVAAEGG SAAVEQVSGP ADAGKSAFVG EELAKSDRPE LTAAKIVVSG GRGMQNGDNF KILYALADKL GAAVGASRAA VDAGFVPNDM QVGQTGKIVA PQLYIAVGIS GAIQHLAGMK DSKVIVAINK DEEAPIFQVA DYGLVADLFD AVPELEKAV

NT Sequence

atggctatcc tggttatcgc tgagcacaac aacgcggccc tggcggctgc caccctgaac accgttgccg ctgccaaggc cattggcggc gacatccacg tgctggtcgc cggacagaac gttgccgccg tggccgaagc cgccgccaag gtcgagggcg tctccaaggt gctggtcgcc gacaacgccg cctacgccca tcaactgccg gaaaacgtcg caccgctgat cgccgagctg ggcaagaact acagccacgt gctggccccg gctaccacca acggcaagaa cttcctgccg cgcgtcgccg cgctgctgga cgtcgaccag atctccgaga tcgtcgaagt ggtcagcccc gacaccttca agcgtccgat ctacgccggt aacgccatcg ctaccgtgca gtcctcggct gcggtcaagg tgatcaccgt gcgtaccacc ggcttcgacg ccgtcgccgc cgaaggcggt tcggctgccg tcgagcaggt ttccggcccg gccgatgccg gcaagtccgc cttcgtcggc gaagagctgg ccaagtccga ccgtccggaa ctgaccgctg ccaagatcgt ggtctccggc ggccgcggca tgcagaacgg cgacaacttc aagatcctct acgccctggc cgacaagctg ggtgccgcag tcggcgcttc ccgcgccgcg gtcgacgccg gtttcgtgcc gaacgacatg caggtcggcc agaccggcaa gatcgtcgcc ccgcagctgt acatcgcggt gggcatctcc ggtgccatcc agcacctggc gggcatgaag gactcgaagg tgatcgtcgc gatcaacaag gacgaagagg ctccgatctt ccaggtcgcc gactacggcc tggtcgccga cctgttcgac gccgtaccgg agctggaaaa ggccgtctga

Details for PsaeA.17867.a.B1.GE38342

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	96

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatggctat cctggttatc gctgagcaca acaacgcggc cctggcggct gccaccctga acaccgttgc cgctgccaag gccattggcg gcgacatcca cgtgctggtc gccggacaga acgttgccgc cgtggccgaa gccgccgcca aggtcgaggg cgtctccaag gtgctggtcg ccgacaacgc cgcctacgcc catcaactgc cggaaaacgt cgcaccgctg atcgccgagc tgggcaagaa ctacagccac gtgctggccc cggctaccac caacggcaag aacttcctgc cgcgcgtcgc cgcgctgctg gacgtcgacc agatctccga gatcgtcgaa gtggtcagcc ccgacacctt caagcgtccg atctacgccg gtaacgccat cgctaccgtg cagtcctcgg ctgcggtcaa ggtgatcacc gtgcgtacca ccggcttcga cgccgtcgcc gccgaaggcg gttcggctgc cgtcgagcag gtttccggcc cggccgatgc cggcaagtcc gccttcgtcg gcgaagagct ggccaagtcc gaccgtccgg aactgaccgc tgccaagatc gtggtctccg gcggccgcgg catgcagaac ggcgacaact tcaagatcct ctacgccctg gccgacaagc tgggtgccgc agtcggcgct tcccgcgccg cggtcgacgc cggtttcgtg ccgaacgaca tgcaggtcgg ccagaccggc aagatcgtcg ccccgcagct gtacatcgcg gtgggcatct ccggtgccat ccagcacctg gcgggcatga aggantcnaa ngtgatcgtc gcgatcaaca anggacgaan aggctccgat cttccaggtc gccnactacg gcctggtcgc cganctgttc nannccntac cgganctnga aa

Expected Protein Sequence

MAHHHHHHMA ILVIAEHNNA ALAAATLNTV AAAKAIGGDI HVLVAGQNVA AVAEAAAKVE GVSKVLVADN AAYAHQLPEN VAPLIAELGK NYSHVLAPAT TNGKNFLPRV AALLDVDQIS EIVEVVSPDT FKRPIYAGNA IATVQSSAAV KVITVRTTGF DAVAAEGGSA AVEQVSGPAD AGKSAFVGEE LAKSDRPELT AAKIVVSGGR GMQNGDNFKI LYALADKLGA AVGASRAAVD AGFVPNDMQV GQTGKIVAPQ LYIAVGISGA IQHLAGMKDS KVIVAINKDE EAPIFQVADY GLVADLFDAV PELEKAV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta tcctggttat cgctgagcac aacaacgcgg ccctggcggc tgccaccctg aacaccgttg ccgctgccaa ggccattggc ggcgacatcc acgtgctggt cgccggacag aacgttgccg ccgtggccga agccgccgcc aaggtcgagg gcgtctccaa ggtgctggtc gccgacaacg ccgcctacgc ccatcaactg ccggaaaacg tcgcaccgct gatcgccgag ctgggcaaga actacagcca cgtgctggcc ccggctacca ccaacggcaa gaacttcctg ccgcgcgtcg ccgcgctgct ggacgtcgac cagatctccg agatcgtcga agtggtcagc cccgacacct tcaagcgtcc gatctacgcc ggtaacgcca tcgctaccgt gcagtcctcg gctgcggtca aggtgatcac cgtgcgtacc accggcttcg acgccgtcgc cgccgaaggc ggttcggctg ccgtcgagca ggtttccggc ccggccgatg ccggcaagtc cgccttcgtc ggcgaagagc tggccaagtc cgaccgtccg gaactgaccg ctgccaagat cgtggtctcc ggcggccgcg gcatgcagaa cggcgacaac ttcaagatcc tctacgccct ggccgacaag ctgggtgccg cagtcggcgc ttcccgcgcc gcggtcgacg ccggtttcgt gccgaacgac atgcaggtcg gccagaccgg caagatcgtc gccccgcagc tgtacatcgc ggtgggcatc tccggtgcca tccagcacct ggcgggcatg aaggactcga aggtgatcgt cgcgatcaac aaggacgaag aggctccgat cttccaggtc gccgactacg gcctggtcgc cgacctgttc gacgccgtac cggagctgga aaaggccgtc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat