SSGCID: Target Page

PsaeA.17866.a

DNA polymerase subunits gamma and tau

CENTER ID:	PsaeA.17866.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17866.a.B1.GE38340	full length		1	681

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.1585
PGAT:	pseudomonas:PA1532
RefSeq:	NP_250223.1
UniProt:	Q9I3I1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYQVLARKW RPRSFREMVG QTHVLKALIN ALDNQRLHHA YLFTGTRGVG KTTIARILAK CLNCETGVSS TPCGECSVCR EIDEGRFVDL IEVDAASRTK VEDTRELLDN VQYSPTRGRY KVYLIDEVHM LSSHSFNALL KTLEEPPPHV KFLLATTDPQ KLPVTILSRC LQFSLKNMPP ERVVEHLTHV LGAENVPFED DALWLLGRAA DGSMRDAMSL TDQAIAFGEG KVLAADVRAM LGTLDHGQVY GVLQALLEGD ARALLEAVRH LAEQGPDWGG VLAEILNVLH RVAIAQALPE AIDNGQGDRE RVLALAQALP AEDVQFYYQM GLIGRRDLPL APDPRSGFEM VLLRMLAFRP ADADGVPRTP LKDLGISKAT TDPANSPVAG AASPAPVATV APAPVVAAPV EAPAAPPAAP SAPPAAVEAR VTEAVVEEPA AAAEVVDLPW EEPAPSLAAE PEPEPEPEPL AVEAPSVPPA VAVEAVVETV LEALPAALPV APDEQDEQDD EPPPADDYYE VDMDTLAYLD ATPEPDVVVV EEPLPAAKPA TGLAAEWLEL FPRLGLGGLT ASIGANCTLV AADDDHWHLH LDPGQSALFN ATQQRRLNDA LNQHLGRTLK LEVTLQKPEQ ETPAQAAARR RAERQRAAEA SIDADPLVRQ LREQFAAVVR DGTIEPLEAK A

NT Sequence

atgagttatc aagttcttgc gcgtaaatgg cgtccccgtt ccttccgtga aatggtcggc cagacccatg tgctcaaggc tctgatcaac gccctggaca accagcggct gcaccatgcc tacctgttca ccggtacccg cggggtcggc aagaccacca tcgcgcggat cctggccaag tgcctgaact gcgagaccgg ggtcagctcc acgccgtgcg gcgaatgctc ggtctgtcgc gaaatcgacg aaggccgctt cgtcgacctg atcgaagtgg acgccgcgag ccggaccaag gtcgaggata cccgcgagct gctcgacaac gtgcagtact cgccgacccg cggacgctac aaggtctacc tgatcgacga agtgcacatg ctctcgtcgc acagtttcaa tgcgctgctc aagaccctcg aggagccgcc gccgcacgtc aagttcctgc tggccaccac cgacccgcag aagttgccgg tgaccatcct ctcgcgctgt ctgcagttct ccctgaagaa catgccgccg gagcgggtgg tcgagcacct gacccacgtg ctcggagccg agaacgtgcc cttcgaggat gacgccctgt ggttgctcgg gcgcgccgcc gacggctcga tgcgtgacgc catgagcctg accgaccagg ccatcgcctt cggcgagggc aaggtgctgg ccgcagacgt gcgggcgatg ctcggtaccc tcgatcacgg ccaggtctat ggcgtgctcc aggccttgct ggagggcgat gcgcgcgccc tgctcgaagc ggtgcgccac ctggccgagc agggcccgga ctggggcggc gtgcttgccg aaatactcaa cgtgctgcac cgcgtcgcga tcgcccaggc cctgccggaa gcgatcgaca atggccaggg cgaccgcgag cgggtgctgg ccctggccca ggcgttgccg gcggaagacg tacagttcta ttaccagatg ggcctgatcg gccgccgcga cctgccgctg gcgcccgatc cgcgtagcgg cttcgagatg gtgttgctgc ggatgctcgc gttccgcccg gcggacgccg acggcgtgcc gaggacgccg ctaaaggacc tggggatcag caaggccacg actgatcccg ccaactcccc cgtggccggc gcggcctcgc ctgcgccggt cgctaccgtt gctcccgcgc ctgtcgtggc tgcgcccgtc gaagccccgg ctgccccgcc ggcagcgcca tccgcgccgc cggcggcggt cgaggcgcgc gtcacggaag cggttgtcga agagcccgcc gccgcagcag aggtcgtcga cttgccctgg gaagagccgg cgccgagcct cgctgccgag cccgagcccg agcccgagcc cgagccgcta gccgtggagg ctccctcggt ccctccggca gtggcggtcg aggcggtggt ggaaaccgtt ctggaagccc tgccggctgc gttgccggta gcacctgacg agcaggacga gcaggacgac gagccaccgc ccgcggacga ctattacgaa gtcgatatgg ataccctggc ctacctggat gccacgcccg agcctgacgt ggtcgtggtg gaggagccgc tgccggcggc gaagccggcc accggcctgg ccgcggagtg gctggagctg ttcccgcggc tcggcctcgg cggcctgacc gccagcatcg gtgccaactg tacgctggta gctgccgacg acgaccactg gcatctgcat ctcgatccgg gccagagcgc gctgttcaac gccacccagc agcggcggct gaacgatgcg ctgaaccagc acctggggcg caccctcaaa ctggaggtga ccctgcagaa gcctgagcag gagaccccgg cccaggcagc ggcacgccgg cgtgccgagc gccagcgggc ggccgaggca tcgatcgatg ccgacccgtt ggtccggcag ttgcgcgagc agttcgccgc ggtggtccgt gacggtacca tcgaaccttt ggaagccaag gcctga

Details for PsaeA.17866.a.B1.GE38340

HARVESTED ON:	data unavailable
SEQUENCED ON:	7/30/2014
EXPECTED MW:	74kDa
OBSERVED MW:	92kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	46

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatgagtta tcaagttctt gcgcgtaaat ggcgtccccg ttccttccgt gaaatggtcg gccagaccca tgtgctcaag gctctgatca acgccctgga caaccagcgg ctgcaccatg cctacctgtt caccggtacc cgcggggtcg gcaagaccac catcgcgcgg atcctggcca agtgcctgaa ctgcgagacc ggggtcagct ccacgccgtg cggcgaatgc tcggtctgtc gcgaaatcga cgaaggccgc ttcgtcgacc tgatcgaagt ggacgccgcg agccggacca aggtcgagga tacccgcgag ctgctcgaca acgtgcagta ctcgccgacc cgcggacgct acaaggtcta cctgatcgac gaagtgcaca tgctctcgtc gcacagtttc aatgcgctgc tcaagaccct cgaggagccg ccgccgcacg tcaagttcct gctggccacc accgacccgc agaagttgcc ggtgaccatc ctctcgcgct gtctgcagtt ctccctgaag aacatgccgc cggagcgggt ggtcgagcac ctgacccacg tgctcggagc cgagaacgtg cccttcgagg atgacgccct gtggttgctc gggcgcgccg ccgacggctc gatgcgtgac gccatgagcc tgaccgacca ggccatcgcc ttcggcgagg gcaaggtgct ggccgcagac gtgcgggcga tgctcggtac cctcgatcac ggccaggtct atggcgtgct ccannccttg ctggagggcg atgcgcgcgc cctgctcgaa gcggtgcgcc acctggnccn agcagggccc ggactggggn cggcgtgctt gccgaaatac tcaacgtgct gcaccgcgtc gcgatcgccc nngccctgnc nnnaagcgat cganaatggc nnnnnnnacc gcnagcgggn gctnnnccng gcccnnnnnt

Expected Protein Sequence

MAHHHHHHMS YQVLARKWRP RSFREMVGQT HVLKALINAL DNQRLHHAYL FTGTRGVGKT TIARILAKCL NCETGVSSTP CGECSVCREI DEGRFVDLIE VDAASRTKVE DTRELLDNVQ YSPTRGRYKV YLIDEVHMLS SHSFNALLKT LEEPPPHVKF LLATTDPQKL PVTILSRCLQ FSLKNMPPER VVEHLTHVLG AENVPFEDDA LWLLGRAADG SMRDAMSLTD QAIAFGEGKV LAADVRAMLG TLDHGQVYGV LQALLEGDAR ALLEAVRHLA EQGPDWGGVL AEILNVLHRV AIAQALPEAI DNGQGDRERV LALAQALPAE DVQFYYQMGL IGRRDLPLAP DPRSGFEMVL LRMLAFRPAD ADGVPRTPLK DLGISKATTD PANSPVAGAA SPAPVATVAP APVVAAPVEA PAAPPAAPSA PPAAVEARVT EAVVEEPAAA AEVVDLPWEE PAPSLAAEPE PEPEPEPLAV EAPSVPPAVA VEAVVETVLE ALPAALPVAP DEQDEQDDEP PPADDYYEVD MDTLAYLDAT PEPDVVVVEE PLPAAKPATG LAAEWLELFP RLGLGGLTAS IGANCTLVAA DDDHWHLHLD PGQSALFNAT QQRRLNDALN QHLGRTLKLE VTLQKPEQET PAQAAARRRA ERQRAAEASI DADPLVRQLR EQFAAVVRDG TIEPLEAKA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt atcaagttct tgcgcgtaaa tggcgtcccc gttccttccg tgaaatggtc ggccagaccc atgtgctcaa ggctctgatc aacgccctgg acaaccagcg gctgcaccat gcctacctgt tcaccggtac ccgcggggtc ggcaagacca ccatcgcgcg gatcctggcc aagtgcctga actgcgagac cggggtcagc tccacgccgt gcggcgaatg ctcggtctgt cgcgaaatcg acgaaggccg cttcgtcgac ctgatcgaag tggacgccgc gagccggacc aaggtcgagg atacccgcga gctgctcgac aacgtgcagt actcgccgac ccgcggacgc tacaaggtct acctgatcga cgaagtgcac atgctctcgt cgcacagttt caatgcgctg ctcaagaccc tcgaggagcc gccgccgcac gtcaagttcc tgctggccac caccgacccg cagaagttgc cggtgaccat cctctcgcgc tgtctgcagt tctccctgaa gaacatgccg ccggagcggg tggtcgagca cctgacccac gtgctcggag ccgagaacgt gcccttcgag gatgacgccc tgtggttgct cgggcgcgcc gccgacggct cgatgcgtga cgccatgagc ctgaccgacc aggccatcgc cttcggcgag ggcaaggtgc tggccgcaga cgtgcgggcg atgctcggta ccctcgatca cggccaggtc tatggcgtgc tccaggcctt gctggagggc gatgcgcgcg ccctgctcga agcggtgcgc cacctggccg agcagggccc ggactggggc ggcgtgcttg ccgaaatact caacgtgctg caccgcgtcg cgatcgccca ggccctgccg gaagcgatcg acaatggcca gggcgaccgc gagcgggtgc tggccctggc ccaggcgttg ccggcggaag acgtacagtt ctattaccag atgggcctga tcggccgccg cgacctgccg ctggcgcccg atccgcgtag cggcttcgag atggtgttgc tgcggatgct cgcgttccgc ccggcggacg ccgacggcgt gccgaggacg ccgctaaagg acctggggat cagcaaggcc acgactgatc ccgccaactc ccccgtggcc ggcgcggcct cgcctgcgcc ggtcgctacc gttgctcccg cgcctgtcgt ggctgcgccc gtcgaagccc cggctgcccc gccggcagcg ccatccgcgc cgccggcggc ggtcgaggcg cgcgtcacgg aagcggttgt cgaagagccc gccgccgcag cagaggtcgt cgacttgccc tgggaagagc cggcgccgag cctcgctgcc gagcccgagc ccgagcccga gcccgagccg ctagccgtgg aggctccctc ggtccctccg gcagtggcgg tcgaggcggt ggtggaaacc gttctggaag ccctgccggc tgcgttgccg gtagcacctg acgagcagga cgagcaggac gacgagccac cgcccgcgga cgactattac gaagtcgata tggataccct ggcctacctg gatgccacgc ccgagcctga cgtggtcgtg gtggaggagc cgctgccggc ggcgaagccg gccaccggcc tggccgcgga gtggctggag ctgttcccgc ggctcggcct cggcggcctg accgccagca tcggtgccaa ctgtacgctg gtagctgccg acgacgacca ctggcatctg catctcgatc cgggccagag cgcgctgttc aacgccaccc agcagcggcg gctgaacgat gcgctgaacc agcacctggg gcgcaccctc aaactggagg tgaccctgca gaagcctgag caggagaccc cggcccaggc agcggcacgc cggcgtgccg agcgccagcg ggcggccgag gcatcgatcg atgccgaccc gttggtccgg cagttgcgcg agcagttcgc cgcggtggtc cgtgacggta ccatcgaacc tttggaagcc aaggcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t