SSGCID: Target Page

PsaeA.17659.a

PcpS

CENTER ID:	PsaeA.17659.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17659.a.B1.GE38339	full length		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.1210
PGAT:	pseudomonas:PA1165
RefSeq:	NP_249856.1
UniProt:	Q9I4H2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRAMNDRLPS FCTPLDDRWP LPVALPGVQL RSTRFDPALL QPGDFALAGI QPPANILRAV AKRQAEFLAG RLCARAALFA LDGRAQTPAV GEDRAPVWPA AISGSITHGD RWAAALVAAR GDWRGLGLDV ETLLEAERAR YLHGEILTEG ERLRFADDLE RRTGLLVTLA FSLKESLFKA LYPLVGKRFY FEHAELLEWR ADGQARLRLL TDLSPEWRHG SELDAQFAVL DGRLLSLVAV GA

NT Sequence

atgcgcgcca tgaacgaccg tctcccctcc ttctgcaccc cgctggacga ccgctggccg ctgcccgtcg cgctgcccgg cgtgcaactg cgcagcaccc gcttcgaccc ggcgctgctg caacccggcg acttcgccct ggccggcatc cagccgccgg cgaacatcct ccgcgcggtg gccaagcgcc aggccgagtt tctcgccggg cgcctctgcg cacgcgccgc cctgttcgcc ctcgatggcc gcgcccagac gccggccgtc ggcgaggacc gcgcaccggt ctggccggcc gcgatcagcg gctcgatcac ccatggcgac cgctgggccg ccgccctggt ggcggcgcgc ggcgactggc gcgggctcgg cctggacgtg gaaaccctgc tcgaagccga acgggcgcgc tacctgcatg gcgagatact caccgaaggc gagcgcctgc gcttcgccga cgacctggag cgacgcaccg gcctgctggt gaccctggcg ttctcgctga aggaaagcct gttcaaggcg ctctatccgc tggtgggcaa gcgcttctat ttcgaacatg cggaactgct ggaatggcgc gccgacggcc aggcccggct gcgcctgctc accgacctgt cgccggagtg gcgccacggc agcgagctgg acgcccagtt cgccgtgctc gacggacgcc tgctcagcct ggtggcggtc ggcgcctga

Details for PsaeA.17659.a.B1.GE38339

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatgcgcgc catgaacgac cgtctcccct ccttctgcac cccgctggac gaccgctggc cgctgcccgt cgcgctgccc ggcgtgcaac tgcgcagcac ccgcttcgac ccggcgctgc tgcaacccgg cgacttcgcc ctggccggca tccagccgcc ggcgaacatc ctccgcgcgg tggccaagcg ccaggccgag tttctcgccg ggcgcctctg cgcacgcgcc gccctgttcg ccctcgatgg ccgcgcccag acgccggccg tcggcgagga ccgcgcaccg gtctggccgg ccgcgatcag cggctcgatc acccatggcg accgctgggc cgccgccctg gtggcggcgc gcggcgactg gcgcgggctc ggcctggacg tggaaaccct gctcgaagcc gaacgggcgc gctacctgca tggcgagata ctcaccgaag gcgagcgcct gcgcttcgcc gacgacctgg agcgacgcac cggcctgctg gtgaccctgg cgttctcgct gaaggaaagc ctgttcaagg cgctctatcc gctggtgggc aagcgcttct atttcgaaca tgcggaactg ctggaatggc gcgccgacgg ccaggcccgg ctgcgcctgc tcaccgacct gtcgccggag tggcgccacg gcagcgagct ggacgcccag ttcgccgtgc tcgacggacg cctgctcagc ctggtggcgg tcggcgccta agtgagtaag atagganccg

Expected Protein Sequence

MAHHHHHHMR AMNDRLPSFC TPLDDRWPLP VALPGVQLRS TRFDPALLQP GDFALAGIQP PANILRAVAK RQAEFLAGRL CARAALFALD GRAQTPAVGE DRAPVWPAAI SGSITHGDRW AAALVAARGD WRGLGLDVET LLEAERARYL HGEILTEGER LRFADDLERR TGLLVTLAFS LKESLFKALY PLVGKRFYFE HAELLEWRAD GQARLRLLTD LSPEWRHGSE LDAQFAVLDG RLLSLVAVGA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg ccatgaacga ccgtctcccc tccttctgca ccccgctgga cgaccgctgg ccgctgcccg tcgcgctgcc cggcgtgcaa ctgcgcagca cccgcttcga cccggcgctg ctgcaacccg gcgacttcgc cctggccggc atccagccgc cggcgaacat cctccgcgcg gtggccaagc gccaggccga gtttctcgcc gggcgcctct gcgcacgcgc cgccctgttc gccctcgatg gccgcgccca gacgccggcc gtcggcgagg accgcgcacc ggtctggccg gccgcgatca gcggctcgat cacccatggc gaccgctggg ccgccgccct ggtggcggcg cgcggcgact ggcgcgggct cggcctggac gtggaaaccc tgctcgaagc cgaacgggcg cgctacctgc atggcgagat actcaccgaa ggcgagcgcc tgcgcttcgc cgacgacctg gagcgacgca ccggcctgct ggtgaccctg gcgttctcgc tgaaggaaag cctgttcaag gcgctctatc cgctggtggg caagcgcttc tatttcgaac atgcggaact gctggaatgg cgcgccgacg gccaggcccg gctgcgcctg ctcaccgacc tgtcgccgga gtggcgccac ggcagcgagc tggacgccca gttcgccgtg ctcgacggac gcctgctcag cctggtggcg gtcggcgcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat