PsaeA.17391.a

RNA polymerase sigma factor RpoD (Sigma-70)

CENTER ID: PsaeA.17391.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17391.a.B1.GE38334 full length 1 617
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.610
PGAT: pseudomonas:PA0576
RefSeq: NP_249267.1
UniProt: P26480

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKAQQQSR LKELIARGRE QGYLTYAEVN DHLPEDISDP EQVEDIIRMI NDMGINVFET APDADALLLA EADTDEAAAE EAAAALAAVE SDIGRTTDPV RMYMREMGTV ELLTREGEIE IAKRIEEGIR EVMSAIAQFP GTVDSILADY NRIVAEGGRL SDVLSGYIDP DDGSLPAEEV EPVNLKDDSA DSKEKDDEEE ESDDSSDSDD EGDGGPDPEE ARLRFTAVSE QLDKAKKALK KHGRGSKQAT AELTGLAELF MPIKLVPKQF DALVARVRSA LEGVRAQERA IMQLCVRDAR MPRADFLRLF PNHETDEKWV DSVLKSKPKY AEAIERLRDD ILRNQQKLAA LESEVELTVA EIKEINRAMS IGEAKARRAK KEMVEANLRL VISIAKKYTN RGLQFLDLIQ EGNIGLMKAV DKFEYRRGYK FSTYATWWIR QAITRSIADQ ARTIRIPVHM IETINKLNRI SRQMLQEMGR EPTPEELGER MDMPEDKIRK VLKIAKEPIS METPIGDDED SHLGDFIEDS TMQSPIEMAT SESLKESTRE VLAGLTAREA KVLRMRFGID MNTDHTLEEV GKQFDVTRER IRQIEAKALR KLRHPSRSEH LRSFLDE
NT Sequence
atgtccggaa aagcgcaaca gcaatctcgt ctgaaagagt tgatcgcccg aggccgtgag cagggatacc tgacttacgc ggaggtcaac gaccacctgc cggaggatat ttccgatccg gaacaggtgg aagacatcat ccgcatgatc aacgacatgg ggatcaacgt attcgagaca gccccggatg cggatgccct gttgctggcc gaggcggata ccgacgaagc cgcagcggaa gaagccgcag cggccctggc cgcggtggaa agcgacatcg gtcgcaccac cgacccggtg cgcatgtaca tgcgtgaaat gggtaccgtg gaactgctga cccgcgaagg cgagatcgaa atcgccaagc gcatcgagga aggcatccgc gaagtgatga gcgccatcgc ccagttcccg ggcacggtgg acagcatcct ggccgactac aatcgcatcg tcgccgaagg cggtcgcctc tccgacgtcc tcagcggcta tatcgatccc gatgacggca gcctgcccgc cgaagaggtg gagccggtca acctgaagga cgattccgcc gactcgaaag agaaggacga cgaggaagaa gaaagcgacg acagcagcga cagcgacgac gaaggcgacg gcggtccgga tccggaagaa gcccgcctgc gtttcaccgc ggtctccgag cagctcgaca aggccaagaa ggccctgaag aagcacggtc gcggcagcaa gcaggccacc gccgaactca ccggcctggc cgagctgttc atgccgatca agctggtgcc caagcagttc gacgccctgg tcgcccgcgt gcgctccgcc ctggaaggcg tgcgcgccca ggaacgcgcc atcatgcagc tctgcgtgcg tgacgcgcgc atgccgcgtg ccgacttcct gcgcctgttc ccgaaccacg agaccgacga gaagtgggtc gacagcgtcc tgaagagcaa gccgaagtac gccgaggcca tcgagcgcct gcgcgacgac atcctgcgca accagcagaa gctggcggcc ctggaaagcg aggtcgagct gaccgtcgcc gagatcaagg aaatcaaccg cgcgatgtcg atcggcgaag ccaaggctcg ccgggcgaag aaggaaatgg tcgaggccaa cctgcgcctg gtgatttcca tcgccaagaa gtacaccaac cgcggcctgc aattcctcga cctgatccag gaaggcaaca tcggcctgat gaaggcggtg gacaagttcg aataccgtcg cggctacaag ttctccacct acgccacctg gtggattcgc caggcgatca cccgttcgat cgccgaccag gcacgcacca tccgcatccc ggtgcacatg atcgagacga tcaacaagct caaccgcatc tcccgccaga tgctccagga aatgggtcgc gagcccaccc cggaagagct tggcgagcgc atggacatgc ctgaggacaa gatccgcaag gtactgaaga tcgccaaaga gccgatctcc atggaaaccc cgatcggtga cgacgaagat tcgcacctgg gcgatttcat cgaggactcc accatgcagt cgccgatcga gatggcgacc agcgagagcc tcaaggaatc cacccgcgaa gtcctcgccg gcctcactgc ccgggaagcc aaggtgctgc gcatgcgctt cggcatcgac atgaacaccg accacaccct cgaggaagtc ggcaagcagt tcgatgtgac ccgcgagcgg atccgccaga tcgaagccaa ggcgctgcgc aagctgcgcc atccgtcgcg aagcgagcac cttcgctcct tcctcgacga gtga
Details for PsaeA.17391.a.B1.GE38334
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 71kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMS GKAQQQSRLK ELIARGREQG YLTYAEVNDH LPEDISDPEQ VEDIIRMIND MGINVFETAP DADALLLAEA DTDEAAAEEA AAALAAVESD IGRTTDPVRM YMREMGTVEL LTREGEIEIA KRIEEGIREV MSAIAQFPGT VDSILADYNR IVAEGGRLSD VLSGYIDPDD GSLPAEEVEP VNLKDDSADS KEKDDEEEES DDSSDSDDEG DGGPDPEEAR LRFTAVSEQL DKAKKALKKH GRGSKQATAE LTGLAELFMP IKLVPKQFDA LVARVRSALE GVRAQERAIM QLCVRDARMP RADFLRXSRT TRPTRSGSTA S
Validated NT Sequence
cnnctagata ttttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg gaaaagcgca acagcaatct cgtctgaaag agttgatcgc ccgaggccgt gagcagggat acctgactta cgcggaggtc aacgaccacc tgccggagga tatttccgat ccggaacagg tggaagacat catccgcatg atcaacgaca tggggatcaa cgtattcgag acagccccgg atgcggatgc cctgttgctg gccgaggcgg ataccgacga agccgcagcg gaagaagccg cagcggccct ggccgcggtg gaaagcgaca tcggtcgcac caccgacccg gtgcgcatgt acatgcgtga aatgggtacc gtggaactgc tgacccgcga aggcgagatc gaaatcgcca agcgcatcga ggaaggcatc cgcgaagtga tgagcgccat cgcccagttc ccgggcacgg tggacagcat cctggccgac tacaatcgca tcgtcgccga aggcggtcgc ctctccgacg tcctcagcgg ctatatcgat cccgatgacg gcagcctgcc cgccgaagag gtggagccgg tcaacctgaa ggacgattcc gccgactcga aagagaagga cgacgaggaa gaagaaagcg acgacagcag cgacagcgac gacgaaggcg acggcggtcc ggatccggaa gaagcccgcc tgcgtttcac cgcggtctcc gagcagctcg acaaggccaa gaaggccctg aagaagcacg gtcgcggcag caagcaggcc accgccgaac tcaccggcct ggccgagctg ttcatgccga tcaagctggt gcccaagcag ttcgacgccc tggtcgcccg cgtgcgctcc gccctggaag gcgtgcgcgc ccaggaacgc gccatcatgc agctctgcgt gcgtgacgcg cgcatgccgc gtgccgactt cctgcgcngt tcccgaacca cgagaccgac nagaagtggg tcgacagcgt cctgaagagc aagccgagta cgncgaggnc atcgagcnnn gcncgacgan nnngnncann gcanaagctg gcngnnnggn ngcgagncga nctgacnnnn cganatnnnn nnancnnnnn nnnnnnntng nnnanncang gnnnnnnnnn nnaaaaannn nnnnnncnan ggnna
Expected Protein Sequence
MAHHHHHHMS GKAQQQSRLK ELIARGREQG YLTYAEVNDH LPEDISDPEQ VEDIIRMIND MGINVFETAP DADALLLAEA DTDEAAAEEA AAALAAVESD IGRTTDPVRM YMREMGTVEL LTREGEIEIA KRIEEGIREV MSAIAQFPGT VDSILADYNR IVAEGGRLSD VLSGYIDPDD GSLPAEEVEP VNLKDDSADS KEKDDEEEES DDSSDSDDEG DGGPDPEEAR LRFTAVSEQL DKAKKALKKH GRGSKQATAE LTGLAELFMP IKLVPKQFDA LVARVRSALE GVRAQERAIM QLCVRDARMP RADFLRLFPN HETDEKWVDS VLKSKPKYAE AIERLRDDIL RNQQKLAALE SEVELTVAEI KEINRAMSIG EAKARRAKKE MVEANLRLVI SIAKKYTNRG LQFLDLIQEG NIGLMKAVDK FEYRRGYKFS TYATWWIRQA ITRSIADQAR TIRIPVHMIE TINKLNRISR QMLQEMGREP TPEELGERMD MPEDKIRKVL KIAKEPISME TPIGDDEDSH LGDFIEDSTM QSPIEMATSE SLKESTREVL AGLTAREAKV LRMRFGIDMN TDHTLEEVGK QFDVTRERIR QIEAKALRKL RHPSRSEHLR SFLDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg gaaaagcgca acagcaatct cgtctgaaag agttgatcgc ccgaggccgt gagcagggat acctgactta cgcggaggtc aacgaccacc tgccggagga tatttccgat ccggaacagg tggaagacat catccgcatg atcaacgaca tggggatcaa cgtattcgag acagccccgg atgcggatgc cctgttgctg gccgaggcgg ataccgacga agccgcagcg gaagaagccg cagcggccct ggccgcggtg gaaagcgaca tcggtcgcac caccgacccg gtgcgcatgt acatgcgtga aatgggtacc gtggaactgc tgacccgcga aggcgagatc gaaatcgcca agcgcatcga ggaaggcatc cgcgaagtga tgagcgccat cgcccagttc ccgggcacgg tggacagcat cctggccgac tacaatcgca tcgtcgccga aggcggtcgc ctctccgacg tcctcagcgg ctatatcgat cccgatgacg gcagcctgcc cgccgaagag gtggagccgg tcaacctgaa ggacgattcc gccgactcga aagagaagga cgacgaggaa gaagaaagcg acgacagcag cgacagcgac gacgaaggcg acggcggtcc ggatccggaa gaagcccgcc tgcgtttcac cgcggtctcc gagcagctcg acaaggccaa gaaggccctg aagaagcacg gtcgcggcag caagcaggcc accgccgaac tcaccggcct ggccgagctg ttcatgccga tcaagctggt gcccaagcag ttcgacgccc tggtcgcccg cgtgcgctcc gccctggaag gcgtgcgcgc ccaggaacgc gccatcatgc agctctgcgt gcgtgacgcg cgcatgccgc gtgccgactt cctgcgcctg ttcccgaacc acgagaccga cgagaagtgg gtcgacagcg tcctgaagag caagccgaag tacgccgagg ccatcgagcg cctgcgcgac gacatcctgc gcaaccagca gaagctggcg gccctggaaa gcgaggtcga gctgaccgtc gccgagatca aggaaatcaa ccgcgcgatg tcgatcggcg aagccaaggc tcgccgggcg aagaaggaaa tggtcgaggc caacctgcgc ctggtgattt ccatcgccaa gaagtacacc aaccgcggcc tgcaattcct cgacctgatc caggaaggca acatcggcct gatgaaggcg gtggacaagt tcgaataccg tcgcggctac aagttctcca cctacgccac ctggtggatt cgccaggcga tcacccgttc gatcgccgac caggcacgca ccatccgcat cccggtgcac atgatcgaga cgatcaacaa gctcaaccgc atctcccgcc agatgctcca ggaaatgggt cgcgagccca ccccggaaga gcttggcgag cgcatggaca tgcctgagga caagatccgc aaggtactga agatcgccaa agagccgatc tccatggaaa ccccgatcgg tgacgacgaa gattcgcacc tgggcgattt catcgaggac tccaccatgc agtcgccgat cgagatggcg accagcgaga gcctcaagga atccacccgc gaagtcctcg ccggcctcac tgcccgggaa gccaaggtgc tgcgcatgcg cttcggcatc gacatgaaca ccgaccacac cctcgaggaa gtcggcaagc agttcgatgt gacccgcgag cggatccgcc agatcgaagc caaggcgctg cgcaagctgc gccatccgtc gcgaagcgag caccttcgct ccttcctcga cgagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat