PsaeA.17389.a
Methionyl-tRNA formyltransferase (EC 2.1.2.9)
| CENTER ID: | PsaeA.17389.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17389.a.B1.GE38333 | full length | 1 | 314 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17389.a.B1.PW37587 | full length | 1 | 314 |
Structures
5UAI
DEPOSITED: 12/19/2016
DETERMINATION: XRay
CLONE: PsaeA.17389.a.B1.GE38333
PROTEIN: PsaeA.17389.a.B1.PW37587
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.17 |
| PGAT: | pseudomonas:PA0018 |
| RefSeq: | NP_248708.1 |
| RefSeq: | WP_003114642.1 |
| UniProt: | O85732 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQALRIVFA GTPEFAAEHL KALLDTPHRI VAVYTQPDRP AGRGQKLMPS AVKSLALEHG LPVMQPQSLR NAEAQAELAA LRADLMVVVA YGLILPQAVL DIPRLGCINS HASLLPRWRG AAPIQRAVEA GDAESGVTVM QMEAGLDTGP MLLKVSTPIS AADTGGSLHD RLAALGPKAV IEAIAGLAAG TLHGEIQDDA LATYAHKLNK DEARLDWSRP AVELERQVRA FTPWPVCHTS LADAPLKVLG ASLGQGSGAP GTILEASRDG LLVACGEGAL RLTRLQLPGG KPLAFADLYN SRREQFAAGQ VLGQ
NT Sequence
atgagccaag cattgcgcat cgtcttcgcc ggaaccccgg aattcgccgc cgagcatctc aaggccctgc tcgacacccc acatcggatc gtcgccgtct acacccagcc tgaccggccg gccggccgcg ggcagaaact gatgcccagc gcggtgaaga gcctggccct ggagcatggc ctgccggtca tgcagccgca gagcctgcgt aatgccgagg cccaggcgga gctggcggcc ctgcgcgcgg acctgatggt ggtggtcgcc tatggcctga tcctgcccca ggcggtactc gatatcccgc gcctgggctg catcaacagc cacgcctcgc tgctgccgcg ctggcgcggc gccgcgccga tccagcgcgc ggtggaagcc ggcgacgcgg agagcggcgt caccgtgatg cagatggaag cagggctcga caccggcccg atgctgctca aggtgagcac gccgatttcc gccgcggaca ccggcggcag cctgcacgat cggctcgccg cgctcggccc gaaagcggtg atcgaagcca tcgccggcct ggccgccggc accctgcatg gcgagatcca ggacgacgcc ctggccacct acgcgcacaa gctgaacaag gacgaggcac gcctcgactg gagccgtccg gccgtcgaac tggagcgcca ggtccgcgcc ttcaccccct ggccggtctg ccacaccagc ctcgccgatg cgccgctgaa agtcctcggc gccagcctgg ggcagggcag cggggcgccc ggaaccatcc tcgaggccag ccgcgacggc ctgctggtcg cctgcggcga aggcgccctg cgcctgaccc gcctgcaatt gcctggcggc aagccactgg ccttcgccga cctctacaac agccgccgcg agcaattcgc cgccggccag gtgctcggcc aatga
Details for PsaeA.17389.a.B1.GE38333
| HARVESTED ON: | 7/21/2014 |
| SEQUENCED ON: | 7/26/2014 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 93 |
| PERCENT COVERAGE: | 101 |
Validated AA Sequence
MAHHHHHHMS QALRIVFAGT PEFAAEHLKA LLDTPHRIVA VYTQPDRPAG RGQKLMPSAV KSLALEHGLP VMQPQSLRNA EAQAELAALR ADLMVVVAYG LILPQAVLDI PRLGCINSHA SLLPRWRGAA PIQRAVEAGD AESGVTVMQM EAGLDTGPML LKVSTPISAA DTGGSLHDRL AALGPKAVIE AIAGLAAGTL HGEIQDDALA TYAHKLNKDE ARLDWSRPAV ELERQVRAFT PWPVCHTSLA DAPLKVLGAS LGQGSGAPGT ILEASRDGLL VACGESALRL TRLQLPGGKP LGPSPTSTTA AASNSPPGQX XRPISE
Validated NT Sequence
gnntnnctct nnatantttt gtttacttta agaaggagat ataccatggc tcaccaccac caccaccata tgagccaagc attgcgcatc gtcttcgccg gaaccccgga attcgccgcc gagcatctca aggccctgct cgacacccca catcggatcg tcgccgtcta cacccagcct gaccggccgg ccggccgcgg gcagaaactg atgcccagcg cggtgaagag cctggccctg gagcatggcc tgccggtcat gcagccgcag agcctgcgta atgccgaggc ccaggcggag ctggcggccc tgcgcgcgga cctgatggtg gtggtcgcct atggcctgat cctgccccag gcggtactcg atatcccgcg cctgggctgc atcaacagcc acgcctcgct gctgccgcgc tggcgcggcg ccgcgccgat ccagcgcgcg gtggaagccg gcgacgcgga gagcggcgtc accgtgatgc agatggaagc agggctcgac accggcccga tgctgctcaa ggtgagcacg ccgatttccg ccgcggacac cggcggcagc ctgcacgatc ggctcgccgc gctcggcccg aaagcggtga tcgaagccat cgccggcctg gccgccggca ccctgcatgg cgagatccag gacgacgccc tggccaccta cgcgcacaag ctgaacaagg acgaggcacg cctcgactgg agccgtccgg ccgtcgaact ggagcgccag gtccgcgcct tcaccccctg gccggtctgc cacaccagcc tcgccgatgc gccgctgaaa gtcctcggcg ccagcctggg gcagggcagc ggggcgcccg gaaccatcct cgaggccagc cgcgacggcc tgctggtcgc ctgcggcgaa agcgccctgc gcctgacccg cctgcaattg cctggcggca agccactggg cccttcgccg acctctacaa cagccgccgc gagcaattcg ccgcccggcc agntgcntcg gccaataagt gagtaagata ggatc
Expected Protein Sequence
MAHHHHHHMS QALRIVFAGT PEFAAEHLKA LLDTPHRIVA VYTQPDRPAG RGQKLMPSAV KSLALEHGLP VMQPQSLRNA EAQAELAALR ADLMVVVAYG LILPQAVLDI PRLGCINSHA SLLPRWRGAA PIQRAVEAGD AESGVTVMQM EAGLDTGPML LKVSTPISAA DTGGSLHDRL AALGPKAVIE AIAGLAAGTL HGEIQDDALA TYAHKLNKDE ARLDWSRPAV ELERQVRAFT PWPVCHTSLA DAPLKVLGAS LGQGSGAPGT ILEASRDGLL VACGEGALRL TRLQLPGGKP LAFADLYNSR REQFAAGQVL GQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc aagcattgcg catcgtcttc gccggaaccc cggaattcgc cgccgagcat ctcaaggccc tgctcgacac cccacatcgg atcgtcgccg tctacaccca gcctgaccgg ccggccggcc gcgggcagaa actgatgccc agcgcggtga agagcctggc cctggagcat ggcctgccgg tcatgcagcc gcagagcctg cgtaatgccg aggcccaggc ggagctggcg gccctgcgcg cggacctgat ggtggtggtc gcctatggcc tgatcctgcc ccaggcggta ctcgatatcc cgcgcctggg ctgcatcaac agccacgcct cgctgctgcc gcgctggcgc ggcgccgcgc cgatccagcg cgcggtggaa gccggcgacg cggagagcgg cgtcaccgtg atgcagatgg aagcagggct cgacaccggc ccgatgctgc tcaaggtgag cacgccgatt tccgccgcgg acaccggcgg cagcctgcac gatcggctcg ccgcgctcgg cccgaaagcg gtgatcgaag ccatcgccgg cctggccgcc ggcaccctgc atggcgagat ccaggacgac gccctggcca cctacgcgca caagctgaac aaggacgagg cacgcctcga ctggagccgt ccggccgtcg aactggagcg ccaggtccgc gccttcaccc cctggccggt ctgccacacc agcctcgccg atgcgccgct gaaagtcctc ggcgccagcc tggggcaggg cagcggggcg cccggaacca tcctcgaggc cagccgcgac ggcctgctgg tcgcctgcgg cgaaggcgcc ctgcgcctga cccgcctgca attgcctggc ggcaagccac tggccttcgc cgacctctac aacagccgcc gcgagcaatt cgccgccggc caggtgctcg gccaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.17389.a.B1.PW37587
| PURIFICATION DATe: | 11/4/2014 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 93 |
| PERCENT COVERAGE: | 101 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS QALRIVFAGT PEFAAEHLKA LLDTPHRIVA VYTQPDRPAG RGQKLMPSAV KSLALEHGLP VMQPQSLRNA EAQAELAALR ADLMVVVAYG LILPQAVLDI PRLGCINSHA SLLPRWRGAA PIQRAVEAGD AESGVTVMQM EAGLDTGPML LKVSTPISAA DTGGSLHDRL AALGPKAVIE AIAGLAAGTL HGEIQDDALA TYAHKLNKDE ARLDWSRPAV ELERQVRAFT PWPVCHTSLA DAPLKVLGAS LGQGSGAPGT ILEASRDGLL VACGESALRL TRLQLPGGKP LGPSPTSTTA AASNSPPGQX XRPISE
Validated NT Sequence
gnntnnctct nnatantttt gtttacttta agaaggagat ataccatggc tcaccaccac caccaccata tgagccaagc attgcgcatc gtcttcgccg gaaccccgga attcgccgcc gagcatctca aggccctgct cgacacccca catcggatcg tcgccgtcta cacccagcct gaccggccgg ccggccgcgg gcagaaactg atgcccagcg cggtgaagag cctggccctg gagcatggcc tgccggtcat gcagccgcag agcctgcgta atgccgaggc ccaggcggag ctggcggccc tgcgcgcgga cctgatggtg gtggtcgcct atggcctgat cctgccccag gcggtactcg atatcccgcg cctgggctgc atcaacagcc acgcctcgct gctgccgcgc tggcgcggcg ccgcgccgat ccagcgcgcg gtggaagccg gcgacgcgga gagcggcgtc accgtgatgc agatggaagc agggctcgac accggcccga tgctgctcaa ggtgagcacg ccgatttccg ccgcggacac cggcggcagc ctgcacgatc ggctcgccgc gctcggcccg aaagcggtga tcgaagccat cgccggcctg gccgccggca ccctgcatgg cgagatccag gacgacgccc tggccaccta cgcgcacaag ctgaacaagg acgaggcacg cctcgactgg agccgtccgg ccgtcgaact ggagcgccag gtccgcgcct tcaccccctg gccggtctgc cacaccagcc tcgccgatgc gccgctgaaa gtcctcggcg ccagcctggg gcagggcagc ggggcgcccg gaaccatcct cgaggccagc cgcgacggcc tgctggtcgc ctgcggcgaa agcgccctgc gcctgacccg cctgcaattg cctggcggca agccactggg cccttcgccg acctctacaa cagccgccgc gagcaattcg ccgcccggcc agntgcntcg gccaataagt gagtaagata ggatc
Expressed Protein Sequence
MAHHHHHHMS QALRIVFAGT PEFAAEHLKA LLDTPHRIVA VYTQPDRPAG RGQKLMPSAV KSLALEHGLP VMQPQSLRNA EAQAELAALR ADLMVVVAYG LILPQAVLDI PRLGCINSHA SLLPRWRGAA PIQRAVEAGD AESGVTVMQM EAGLDTGPML LKVSTPISAA DTGGSLHDRL AALGPKAVIE AIAGLAAGTL HGEIQDDALA TYAHKLNKDE ARLDWSRPAV ELERQVRAFT PWPVCHTSLA DAPLKVLGAS LGQGSGAPGT ILEASRDGLL VACGEGALRL TRLQLPGGKP LAFADLYNSR REQFAAGQVL GQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC AAGCATTGCG CATCGTCTTC GCCGGAACCC CGGAATTCGC CGCCGAGCAT CTCAAGGCCC TGCTCGACAC CCCACATCGG ATCGTCGCCG TCTACACCCA GCCTGACCGG CCGGCCGGCC GCGGGCAGAA ACTGATGCCC AGCGCGGTGA AGAGCCTGGC CCTGGAGCAT GGCCTGCCGG TCATGCAGCC GCAGAGCCTG CGTAATGCCG AGGCCCAGGC GGAGCTGGCG GCCCTGCGCG CGGACCTGAT GGTGGTGGTC GCCTATGGCC TGATCCTGCC CCAGGCGGTA CTCGATATCC CGCGCCTGGG CTGCATCAAC AGCCACGCCT CGCTGCTGCC GCGCTGGCGC GGCGCCGCGC CGATCCAGCG CGCGGTGGAA GCCGGCGACG CGGAGAGCGG CGTCACCGTG ATGCAGATGG AAGCAGGGCT CGACACCGGC CCGATGCTGC TCAAGGTGAG CACGCCGATT TCCGCCGCGG ACACCGGCGG CAGCCTGCAC GATCGGCTCG CCGCGCTCGG CCCGAAAGCG GTGATCGAAG CCATCGCCGG CCTGGCCGCC GGCACCCTGC ATGGCGAGAT CCAGGACGAC GCCCTGGCCA CCTACGCGCA CAAGCTGAAC AAGGACGAGG CACGCCTCGA CTGGAGCCGT CCGGCCGTCG AACTGGAGCG CCAGGTCCGC GCCTTCACCC CCTGGCCGGT CTGCCACACC AGCCTCGCCG ATGCGCCGCT GAAAGTCCTC GGCGCCAGCC TGGGGCAGGG CAGCGGGGCG CCCGGAACCA TCCTCGAGGC CAGCCGCGAC GGCCTGCTGG TCGCCTGCGG CGAAGGCGCC CTGCGCCTGA CCCGCCTGCA ATTGCCTGGC GGCAAGCCAC TGGCCTTCGC CGACCTCTAC AACAGCCGCC GCGAGCAATT CGCCGCCGGC CAGGTGCTCG GCCAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT