PsaeA.17385.a
Uncharacterized protein (HHPRED: peptidase YEAZ)
| CENTER ID: | PsaeA.17385.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17385.a.B1.GE38332 | full length | 1 | 226 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5BR9
DEPOSITED: 5/29/2015
DETERMINATION: XRay
CLONE: PsaeA.17385.a.B1.GE38332
PROTEIN: PsaeA.17385.a.B1.PS02242
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.3854 |
| PGAT: | pseudomonas:PA3685 |
| RefSeq: | NP_252375.1 |
| UniProt: | Q9HXV5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLLALDTS TEACSVALLH EGRALSHYEV IPRLHAQRLL PMVRDLLDEA GVALSAVDAI AFGRGPGAFT GVRIAIGVVQ GLAFALQRPV LAVSDLAILA QRAYREQGAE RVAAAIDARM DEVYWGCYQL QQGEMRLAGS EAVLPPERVA VPWDAAAADW FGAGTGWGYV ERMPQRPVAL DASLLPHAED LLSLAGFAWA RGEGVEAEQA LPVYLRDNVA TPKKAP
NT Sequence
atgtccactc tgttggccct ggatacctcc accgaagcct gctccgtcgc cctgctccac gaggggcggg cgctcagtca ctacgaggtg atcccgcgcc tgcacgccca gcgcctgctg ccgatggtcc gcgacctgct cgacgaggcc ggcgtcgccc tgtccgcggt ggacgccatc gccttcgggc gcggtccggg cgccttcacc ggcgtgcgca tcgccatcgg cgtggtccag ggcctggcct tcgccctgca gcgcccggta ctggcggtct ccgacctggc gatcctggcc cagcgggcct accgcgagca gggtgcggag cgggtcgccg cggccatcga cgcgcggatg gacgaggtct actggggttg ctaccagttg caacagggcg agatgcgcct ggccggcagc gaagcggtac tgccgcccga gcgggtcgcc gtaccgtggg acgccgctgc cgcggactgg ttcggcgccg gcaccggctg gggctacgtc gagcgcatgc cgcagcgccc ggtcgcgctg gacgcgagcc tgctgccgca tgccgaggac ttgctcagcc tggccggctt cgcctgggcg cgcggcgaag gcgtggaggc ggaacaggcg ctgccggtgt acctgcgcga caacgtggcg acgccgaaaa aagcgccctg a
Details for PsaeA.17385.a.B1.GE38332
| HARVESTED ON: | 7/21/2014 |
| SEQUENCED ON: | 7/26/2014 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS TLLALDTSTE ACSVALLHEG RALSHYEVIP RLHAQRLLPM VRDLLDEAGV ALSAVDAIAF GRGPGAFTGV RIAIGVVQGL AFALQRPVLA VSDLAILAQR AYREQGAERV AAAIDARMDE VYWGCYQLQQ GEMRLAGSEA VLPPERVAVP WDAAAADWFG AGTGWGYVER MPQRPVALDA SLLPHAEDLL SLAGFAWARG EGVEAEQALP VYLRDNVATP KKAP
Validated NT Sequence
tcntctanat attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgtc cactctgttg gccctggata cctccaccga agcctgctcc gtcgccctgc tccacgaggg gcgggcgctc agtcactacg aggtgatccc gcgcctgcac gcccagcgcc tgctgccgat ggtccgcgac ctgctcgacg aggccggcgt cgccctgtcc gcggtggacg ccatcgcctt cgggcgcggt ccgggcgcct tcaccggcgt gcgcatcgcc atcggcgtgg tccagggcct ggccttcgcc ctgcagcgcc cggtactggc ggtctccgac ctggcgatcc tggcccagcg ggcctaccgc gagcagggtg cggagcgggt cgccgcggcc atcgacgcgc ggatggacga ggtctactgg ggttgctacc agttgcaaca gggcgagatg cgcctggccg gcagcgaagc ggtactgccg cccgagcggg tcgccgtacc gtgggacgcc gctgccgcgg actggttcgg cgccggcacc ggctggggct acgtcgagcg catgccgcag cgcccggtcg cgctggacgc gagcctgctg ccgcatgccg aggacttgct cagcctggcc ggcttcgcct gggcgcgcgg cgaaggcgtg gaggcggaac aggcgctgcc ggtgtacctg cgcgacaacg tggcgacgcc gaaaaaagcg ccctaagtga gtaagatagg atccggctgn acnaaccccc ccnaaannna gggannaann nnnncnnccg nnnntggtgg caacctggcc gccgccnctc gcgcgaacgc attcanaacg ccgnccacna acngctggcg ctgccctncc ccgagggcca cgtcaatttg cacaagctgc ctcctggaac tgncggcccn gcggcatcaa cgagcgnttc ttggaggccg tcccncantc tngtccngcg cctntcccgg gctgggnctt ggtggatnag tacacgncta gtcnnancgc tcngactanc tnngggttcn ctggaccc
Expected Protein Sequence
MAHHHHHHMS TLLALDTSTE ACSVALLHEG RALSHYEVIP RLHAQRLLPM VRDLLDEAGV ALSAVDAIAF GRGPGAFTGV RIAIGVVQGL AFALQRPVLA VSDLAILAQR AYREQGAERV AAAIDARMDE VYWGCYQLQQ GEMRLAGSEA VLPPERVAVP WDAAAADWFG AGTGWGYVER MPQRPVALDA SLLPHAEDLL SLAGFAWARG EGVEAEQALP VYLRDNVATP KKAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca ctctgttggc cctggatacc tccaccgaag cctgctccgt cgccctgctc cacgaggggc gggcgctcag tcactacgag gtgatcccgc gcctgcacgc ccagcgcctg ctgccgatgg tccgcgacct gctcgacgag gccggcgtcg ccctgtccgc ggtggacgcc atcgccttcg ggcgcggtcc gggcgccttc accggcgtgc gcatcgccat cggcgtggtc cagggcctgg ccttcgccct gcagcgcccg gtactggcgg tctccgacct ggcgatcctg gcccagcggg cctaccgcga gcagggtgcg gagcgggtcg ccgcggccat cgacgcgcgg atggacgagg tctactgggg ttgctaccag ttgcaacagg gcgagatgcg cctggccggc agcgaagcgg tactgccgcc cgagcgggtc gccgtaccgt gggacgccgc tgccgcggac tggttcggcg ccggcaccgg ctggggctac gtcgagcgca tgccgcagcg cccggtcgcg ctggacgcga gcctgctgcc gcatgccgag gacttgctca gcctggccgg cttcgcctgg gcgcgcggcg aaggcgtgga ggcggaacag gcgctgccgg tgtacctgcg cgacaacgtg gcgacgccga aaaaagcgcc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat