PsaeA.17380.a

Riboflavin biosynthesis protein RibD

CENTER ID: PsaeA.17380.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17380.a.B1.GE38331 full length 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4247
PGAT: pseudomonas:PA4056
RefSeq: NP_252745.1
UniProt: Q9HWX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAADPLYMA RALELARQGL YSTQPNPRVG CVLVKDGQVV GEGWHVRAGE PHAEVHALRQ AGENARGATA YVTLEPCSHF GRTPPCADAL VGAGVARVVA AMQDPNPEVA GRGLLRLMQA GIAVQSGVLE AEARELNIGF IKRMEHGLPF VRVKLAMSLD GRTAMASGES QWITGPSARS AVQRLRARSS VVLSGADTLL ADDARLNVRP DELGLNAELT ALAAARQPLR VLVDGRLRVP LESAFYQAGP ALVATCAAAS ARERFQDAGH ELLALPSAEG HVDLHKLLLE LAARGANEVL VEAGPRLAGA FARLGLVDEY RLFVAPKFLG SSARPLLDWP LARMADAAQL RIDDIRAVGD DWLVTARPHL RDA
NT Sequence
atgaacgccg ccgacccgct gtacatggcc cgcgccctgg aactggcgcg ccaggggctg tactccaccc agccgaatcc gcgcgtcggc tgcgtgctgg tcaaggacgg ccaggtggtc ggcgagggct ggcacgtccg cgccggcgag ccgcacgccg aggtccatgc cctgcgccag gccggcgaga atgcacgcgg cgccaccgcc tacgtgaccc tcgagccctg cagccacttc ggccgcaccc cgccgtgcgc cgatgcgctg gtgggcgccg gggtggcgcg ggtggtcgcg gcgatgcagg atcccaaccc cgaggtcgcc ggtcgcggcc tgctgcgtct gatgcaggcc ggcatcgcgg tgcagagcgg cgtcctcgaa gccgaggcgc gggaactcaa catcggtttc atcaagcgca tggaacatgg cctgccgttc gtccgggtca agctggcgat gagcctggac gggcgcaccg ccatggccag cggggaaagc cagtggatca ccgggccctc cgcgcgttcg gcggtgcagc gcttgcgcgc tcgctccagc gtggtgttgt ccggcgccga tacgctgctg gcggacgatg cgcggctcaa cgtacgcccc gacgaactgg gcctgaacgc cgagcttacc gcgctcgccg cggctcgcca gccgttgcgg gtgctggtcg acgggcgcct gcgggtgccg ctggagtcgg cgttctacca ggccggtccg gcgctggtgg cgacctgcgc cgccgcctcg gcgcgcgaac gcttccagga cgccggccac gaactgctgg cgctgccctc cgccgagggc cacgtcgatt tgcacaagct gctcctggaa ctggcggcgc gcggcgccaa cgaagtgctg gtggaggccg gcccgcgtct ggccggcgcc ttcgcccggc tggggctggt ggatgagtac cggctgttcg tcgcgccgaa gttcctcggc tccagcgccc gtcctttgct cgattggccg ctggcgcgga tggccgatgc ggcgcagttg cggatcgacg acatccgcgc ggtgggcgac gactggctgg tcaccgcgcg gccccatctg cgggacgcct ga
Details for PsaeA.17380.a.B1.GE38331
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 41kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMN AADPLYMARA LELARQGLYS TQPNPRVGCV LVKDGQVVGE GWHVRAGEPH AEVHALRQAG ENARGATAYV TLEPCSHFGR TPPCADALVG AGVARVVAAM QDPNPEVAGR GLLRLMQAGI AVQSGVLEAE ARELNIGFIK RMEHGLPFVR VKLAMSLDGR TAMASGESQW ITGPSARSAV QRLRARSSVV LSGADTLLAD DARLNVRPDE LGLNAELTAL AAARQPLRVL VDGRLRVPLE SAFYQAGPAL VATCAAASAR ERFQDAGHEL LALPSAEGHV DLHKLLLELA ARGATKCWVE PGPRLGXLRP AGAGG
Validated NT Sequence
gnnnntnnnt ctanatattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgaacgcc gccgacccgc tgtacatggc ccgcgccctg gaactggcgc gccaggggct gtactccacc cagccgaatc cgcgcgtcgg ctgcgtgctg gtcaaggacg gccaggtggt cggcgagggc tggcacgtcc gcgccggcga gccgcacgcc gaggtccatg ccctgcgcca ggccggcgag aatgcacgcg gcgccaccgc ctacgtgacc ctcgagccct gcagccactt cggccgcacc ccgccgtgcg ccgatgcgct ggtgggcgcc ggggtggcgc gggtggtcgc ggcgatgcag gatcccaacc ccgaggtcgc cggtcgcggc ctgctgcgtc tgatgcaggc cggcatcgcg gtgcagagcg gcgtcctcga agccgaggcg cgggaactca acatcggttt catcaagcgc atggaacatg gcctgccgtt cgtccgggtc aagctggcga tgagcctgga cgggcgcacc gccatggcca gcggggaaag ccagtggatc accgggccct ccgcgcgttc ggcggtgcag cgcttgcgcg ctcgctccag cgtggtgttg tccggcgccg atacgctgct ggcggacgat gcgcggctca acgtacgccc cgacgaactg ggcctgaacg ccgagcttac cgcgctcgcc gcggctcgcc agccgttgcg ggtgctggtc gacgggcgcc tgcgggtgcc gctggagtcg gcgttctacc aggccggtcc ggcgctggtg gcgacctgcg ccgccgcctc ggcgcgcgaa cgcttccagg acgccggcca cgaactgctg gcgctgccct ccgccgaggg ccacgtcgat ttgcacaagc tgctcctgga actggcggcg cgcggcgcca cgaagtgctg ggtggagccg ggcccgcgtc tgggcncgct tcgcccggct ggggctggtg gatgagtacc ggctggtcgt cgcgccgaan ttnctnnnnc ngcgnccgtc nttgctcgat tggcnnctgg cgcgnangcc nnatnnnnnn nnntgtcgga ntnnnnnnna nntncngnnn ggggcgnnnn actgntgnnn ngnnnnnncn ntnnnnnnnn nnnnnnnnnn ngnnncnnnn nananannnn nnnnngagna nncnag
Expected Protein Sequence
MAHHHHHHMN AADPLYMARA LELARQGLYS TQPNPRVGCV LVKDGQVVGE GWHVRAGEPH AEVHALRQAG ENARGATAYV TLEPCSHFGR TPPCADALVG AGVARVVAAM QDPNPEVAGR GLLRLMQAGI AVQSGVLEAE ARELNIGFIK RMEHGLPFVR VKLAMSLDGR TAMASGESQW ITGPSARSAV QRLRARSSVV LSGADTLLAD DARLNVRPDE LGLNAELTAL AAARQPLRVL VDGRLRVPLE SAFYQAGPAL VATCAAASAR ERFQDAGHEL LALPSAEGHV DLHKLLLELA ARGANEVLVE AGPRLAGAFA RLGLVDEYRL FVAPKFLGSS ARPLLDWPLA RMADAAQLRI DDIRAVGDDW LVTARPHLRD A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg ccgccgaccc gctgtacatg gcccgcgccc tggaactggc gcgccagggg ctgtactcca cccagccgaa tccgcgcgtc ggctgcgtgc tggtcaagga cggccaggtg gtcggcgagg gctggcacgt ccgcgccggc gagccgcacg ccgaggtcca tgccctgcgc caggccggcg agaatgcacg cggcgccacc gcctacgtga ccctcgagcc ctgcagccac ttcggccgca ccccgccgtg cgccgatgcg ctggtgggcg ccggggtggc gcgggtggtc gcggcgatgc aggatcccaa ccccgaggtc gccggtcgcg gcctgctgcg tctgatgcag gccggcatcg cggtgcagag cggcgtcctc gaagccgagg cgcgggaact caacatcggt ttcatcaagc gcatggaaca tggcctgccg ttcgtccggg tcaagctggc gatgagcctg gacgggcgca ccgccatggc cagcggggaa agccagtgga tcaccgggcc ctccgcgcgt tcggcggtgc agcgcttgcg cgctcgctcc agcgtggtgt tgtccggcgc cgatacgctg ctggcggacg atgcgcggct caacgtacgc cccgacgaac tgggcctgaa cgccgagctt accgcgctcg ccgcggctcg ccagccgttg cgggtgctgg tcgacgggcg cctgcgggtg ccgctggagt cggcgttcta ccaggccggt ccggcgctgg tggcgacctg cgccgccgcc tcggcgcgcg aacgcttcca ggacgccggc cacgaactgc tggcgctgcc ctccgccgag ggccacgtcg atttgcacaa gctgctcctg gaactggcgg cgcgcggcgc caacgaagtg ctggtggagg ccggcccgcg tctggccggc gccttcgccc ggctggggct ggtggatgag taccggctgt tcgtcgcgcc gaagttcctc ggctccagcg cccgtccttt gctcgattgg ccgctggcgc ggatggccga tgcggcgcag ttgcggatcg acgacatccg cgcggtgggc gacgactggc tggtcaccgc gcggccccat ctgcgggacg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat