PsaeA.17369.c

ATP synthase subunit alpha (EC 3.6.3.14) (ATP synthase F1 sector subunit alpha) (F-ATPase subunit alpha)

CENTER ID: PsaeA.17369.c
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17369.c.B1.GE38330 full length 1 514
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17369.c.B1.PS02338 full length 1 514

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5822
PGAT: pseudomonas:PA5556
RefSeq: NP_254243.1
UniProt: Q9HT18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQLNPSEIS EIIKGRIEKL DVASQARNEG TIVSVSDGIV RIYGLADVMY GEMIEFPGGV YGMALNLEQD SVGAVVLGEY QGLAEGMNAK CTGRILEVPV GPELLGRVVD ALGNPIDGKG PIDAKATDAV EKVAPGVIWR KSVDQPVQTG YKSVDAMIPV GRGQRELIIG DRQIGKTALA VDAIINQKDS GIKCVYVAIG QKQSTIANVV RKLEENGALA NTIVVAASAS ESAALQYLAP YSGCTMGEYF RDRGEDALIV YDDLSKQAVA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RASRVSEEYV EKFTNGAVTG KTGSLTALPI IETQAGDVSA FVPTNVISIT DGQIFLESAM FNSGIRPAVN AGISVSRVGG AAQTKIIKKL SGGIRTALAQ YRELAAFAQF ASDLDEATRK QLEHGQRVTE LMKQKQYAPM SIAEMSLSLY AAERGFLQDV EIAKVGSFEQ ALISYFQREH AALLAKINEK GDFNDEIDAG IKAGIEKFKA TQTW
NT Sequence
atgcagcaac tcaatccttc cgaaattagt gaaatcatca aggggcgcat cgagaaactc gacgtcgcct cccaagcacg aaacgaaggc accatcgtca gcgtgtccga cggtatcgtg cgcatctacg gtctggccga cgtcatgtac ggcgagatga tcgagttccc gggcggcgtc tacggtatgg cgctgaacct ggagcaggat tccgtgggtg ccgtggtcct gggtgaatac cagggcctcg ccgaaggcat gaacgccaag tgcaccgggc gcatcctgga ggttccggtc ggtccggaac tgctgggtcg cgtagtcgac gcactgggca atccgatcga cggcaagggt ccgatcgacg ccaaggccac cgacgcggtc gagaaggtcg ctccgggcgt gatctggcgt aagtcggtag accagccggt acagaccggc tacaagtcgg tcgacgccat gatcccggta ggccgtggcc agcgcgagct gatcatcggc gaccgccaga tcggcaagac cgccctcgcg gtcgacgcca tcatcaacca gaaagacagc ggcattaagt gcgtctacgt ggccatcggc cagaagcagt ccactatcgc caacgtcgta cgcaagctgg aagagaacgg tgccctggcc aacaccatcg tggtcgccgc cagcgcttcc gagtccgccg ccctgcaata cctggcgccg tactccggtt gcaccatggg cgaatacttc cgcgatcgcg gtgaagacgc cctgatcgtc tacgacgacc tgtccaagca ggccgtggct tatcgccaga tctccctgct gctgcgccgt ccgccgggtc gcgaagccta cccgggcgac gtgttctatc tccacagccg tctgctggag cgcgcttccc gcgtttccga agagtatgtc gagaagttca ccaacggcgc cgtgaccggc aagaccggct cgctgaccgc gctgccgatc atcgagaccc aggccggcga cgtttccgcg ttcgttccga ccaacgtgat ctcgatcacc gacggccaga tcttcctgga atccgccatg ttcaactcgg gtatccgtcc ggccgtcaac gccggtatct cggtatcccg cgtaggtggc gcggcccaga ccaagatcat caagaagctg tccggcggca tccgtaccgc tctggcccag taccgtgaac tggcggcctt cgcccagttc gcctcggacc tggacgaagc gacccgcaag cagctggagc atggtcagcg cgttaccgag ctgatgaagc agaagcagta cgcgccgatg tcgatcgccg agatgtcgct gtccctgtat gccgccgagc gtggcttcct gcaggacgtc gaaatcgcca aggtcggtag cttcgagcag gcgttgatct cctacttcca gcgcgagcat gccgctctgc tggcgaagat caacgagaaa ggtgacttca acgacgaaat cgacgcgggc atcaaggccg gcatcgagaa gttcaaggcc acccaaacct ggtaa
Details for PsaeA.17369.c.B1.GE38330
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 56kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 59
Validated AA Sequence
MAHHHHHHMQ QLNPSEISEI IKGRIEKLDV ASQARNEGTI VSVSDGIVRI YGLADVMYGE MIEFPGGVYG MALNLEQDSV GAVVLGEYQG LAEGMNAKCT GRILEVPVGP ELLGRVVDAL GNPIDGKGPI DAKATDAVEK VAPGVIWRKS VDQPVQTGYK SVDAMIPVGR GQRELIIGDR QIGKTALAVD AIINQKDSGI KCVYVAIGQK QSTIANVVRK LEENGALANT IVVAASASES AALQYLAPYS GCTMGEYFRD RGEDALIVYD DLSKQAVAYR QISLLLRRPP GREAYPGDVF YLHSRLLERA SRV
Validated NT Sequence
atggctcacc accaccacca ccatatgcag caactcaatc cttccgaaat tagtgaaatc atcaaggggc gcatcgagaa actcgacgtc gcctcccaag cacgaaacga aggcaccatc gtcagcgtgt ccgacggtat cgtgcgcatc tacggtctgg ccgacgtcat gtacggcgag atgatcgagt tcccgggcgg cgtctacggt atggcgctga acctggagca ggattccgtg ggtgccgtgg tcctgggtga ataccagggc ctcgccgaag gcatgaacgc caagtgcacc gggcgcatcc tggaggttcc ggtcggtccg gaactgctgg gtcgcgtagt cgacgcactg ggcaatccga tcgacggcaa gggtccgatc gacgccaagg ccaccgacgc ggtcgagaag gtcgctccgg gcgtgatctg gcgtaagtcg gtagaccagc cggtacagac cggctacaag tcggtcgacg ccatgatccc ggtaggccgt ggccagcgcg agctgatcat cggcgaccgc cagatcggca agaccgccct cgcggtcgac gccatcatca accagaaaga cagcggcatt aagtgcgtct acgtggccat cggccagaag cagtccacta tcgccaacgt cgtacgcaag ctggaagaga acggtgccct ggccaacacc atcgtggtcg ccgccagcgc ttccgagtcc gccgccctgc aatacctggc gccgtactcc ggttgcacca tgggcgaata cttccgcgat cgcggtgaag acgccctgat cgtctacgac gacctgtcca agcaggccgt ggcttatcgc cagatctccc tgctgctgcg ccgtccgccg ggtcgcgaag cctacccggg cgacgtgttc tatctccaca gccgtctgct ggagcgcgct tcccgcgttt cc
Expected Protein Sequence
MAHHHHHHMQ QLNPSEISEI IKGRIEKLDV ASQARNEGTI VSVSDGIVRI YGLADVMYGE MIEFPGGVYG MALNLEQDSV GAVVLGEYQG LAEGMNAKCT GRILEVPVGP ELLGRVVDAL GNPIDGKGPI DAKATDAVEK VAPGVIWRKS VDQPVQTGYK SVDAMIPVGR GQRELIIGDR QIGKTALAVD AIINQKDSGI KCVYVAIGQK QSTIANVVRK LEENGALANT IVVAASASES AALQYLAPYS GCTMGEYFRD RGEDALIVYD DLSKQAVAYR QISLLLRRPP GREAYPGDVF YLHSRLLERA SRVSEEYVEK FTNGAVTGKT GSLTALPIIE TQAGDVSAFV PTNVISITDG QIFLESAMFN SGIRPAVNAG ISVSRVGGAA QTKIIKKLSG GIRTALAQYR ELAAFAQFAS DLDEATRKQL EHGQRVTELM KQKQYAPMSI AEMSLSLYAA ERGFLQDVEI AKVGSFEQAL ISYFQREHAA LLAKINEKGD FNDEIDAGIK AGIEKFKATQ TW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc aactcaatcc ttccgaaatt agtgaaatca tcaaggggcg catcgagaaa ctcgacgtcg cctcccaagc acgaaacgaa ggcaccatcg tcagcgtgtc cgacggtatc gtgcgcatct acggtctggc cgacgtcatg tacggcgaga tgatcgagtt cccgggcggc gtctacggta tggcgctgaa cctggagcag gattccgtgg gtgccgtggt cctgggtgaa taccagggcc tcgccgaagg catgaacgcc aagtgcaccg ggcgcatcct ggaggttccg gtcggtccgg aactgctggg tcgcgtagtc gacgcactgg gcaatccgat cgacggcaag ggtccgatcg acgccaaggc caccgacgcg gtcgagaagg tcgctccggg cgtgatctgg cgtaagtcgg tagaccagcc ggtacagacc ggctacaagt cggtcgacgc catgatcccg gtaggccgtg gccagcgcga gctgatcatc ggcgaccgcc agatcggcaa gaccgccctc gcggtcgacg ccatcatcaa ccagaaagac agcggcatta agtgcgtcta cgtggccatc ggccagaagc agtccactat cgccaacgtc gtacgcaagc tggaagagaa cggtgccctg gccaacacca tcgtggtcgc cgccagcgct tccgagtccg ccgccctgca atacctggcg ccgtactccg gttgcaccat gggcgaatac ttccgcgatc gcggtgaaga cgccctgatc gtctacgacg acctgtccaa gcaggccgtg gcttatcgcc agatctccct gctgctgcgc cgtccgccgg gtcgcgaagc ctacccgggc gacgtgttct atctccacag ccgtctgctg gagcgcgctt cccgcgtttc cgaagagtat gtcgagaagt tcaccaacgg cgccgtgacc ggcaagaccg gctcgctgac cgcgctgccg atcatcgaga cccaggccgg cgacgtttcc gcgttcgttc cgaccaacgt gatctcgatc accgacggcc agatcttcct ggaatccgcc atgttcaact cgggtatccg tccggccgtc aacgccggta tctcggtatc ccgcgtaggt ggcgcggccc agaccaagat catcaagaag ctgtccggcg gcatccgtac cgctctggcc cagtaccgtg aactggcggc cttcgcccag ttcgcctcgg acctggacga agcgacccgc aagcagctgg agcatggtca gcgcgttacc gagctgatga agcagaagca gtacgcgccg atgtcgatcg ccgagatgtc gctgtccctg tatgccgccg agcgtggctt cctgcaggac gtcgaaatcg ccaaggtcgg tagcttcgag caggcgttga tctcctactt ccagcgcgag catgccgctc tgctggcgaa gatcaacgag aaaggtgact tcaacgacga aatcgacgcg ggcatcaagg ccggcatcga gaagttcaag gccacccaaa cctggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.17369.c.B1.PS02338
PURIFICATION DATe: 2/18/2015
CONCENTRATION: 57.16mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 59
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ QLNPSEISEI IKGRIEKLDV ASQARNEGTI VSVSDGIVRI YGLADVMYGE MIEFPGGVYG MALNLEQDSV GAVVLGEYQG LAEGMNAKCT GRILEVPVGP ELLGRVVDAL GNPIDGKGPI DAKATDAVEK VAPGVIWRKS VDQPVQTGYK SVDAMIPVGR GQRELIIGDR QIGKTALAVD AIINQKDSGI KCVYVAIGQK QSTIANVVRK LEENGALANT IVVAASASES AALQYLAPYS GCTMGEYFRD RGEDALIVYD DLSKQAVAYR QISLLLRRPP GREAYPGDVF YLHSRLLERA SRV
Validated NT Sequence
atggctcacc accaccacca ccatatgcag caactcaatc cttccgaaat tagtgaaatc atcaaggggc gcatcgagaa actcgacgtc gcctcccaag cacgaaacga aggcaccatc gtcagcgtgt ccgacggtat cgtgcgcatc tacggtctgg ccgacgtcat gtacggcgag atgatcgagt tcccgggcgg cgtctacggt atggcgctga acctggagca ggattccgtg ggtgccgtgg tcctgggtga ataccagggc ctcgccgaag gcatgaacgc caagtgcacc gggcgcatcc tggaggttcc ggtcggtccg gaactgctgg gtcgcgtagt cgacgcactg ggcaatccga tcgacggcaa gggtccgatc gacgccaagg ccaccgacgc ggtcgagaag gtcgctccgg gcgtgatctg gcgtaagtcg gtagaccagc cggtacagac cggctacaag tcggtcgacg ccatgatccc ggtaggccgt ggccagcgcg agctgatcat cggcgaccgc cagatcggca agaccgccct cgcggtcgac gccatcatca accagaaaga cagcggcatt aagtgcgtct acgtggccat cggccagaag cagtccacta tcgccaacgt cgtacgcaag ctggaagaga acggtgccct ggccaacacc atcgtggtcg ccgccagcgc ttccgagtcc gccgccctgc aatacctggc gccgtactcc ggttgcacca tgggcgaata cttccgcgat cgcggtgaag acgccctgat cgtctacgac gacctgtcca agcaggccgt ggcttatcgc cagatctccc tgctgctgcg ccgtccgccg ggtcgcgaag cctacccggg cgacgtgttc tatctccaca gccgtctgct ggagcgcgct tcccgcgttt cc
Expressed Protein Sequence
MAHHHHHHMQ QLNPSEISEI IKGRIEKLDV ASQARNEGTI VSVSDGIVRI YGLADVMYGE MIEFPGGVYG MALNLEQDSV GAVVLGEYQG LAEGMNAKCT GRILEVPVGP ELLGRVVDAL GNPIDGKGPI DAKATDAVEK VAPGVIWRKS VDQPVQTGYK SVDAMIPVGR GQRELIIGDR QIGKTALAVD AIINQKDSGI KCVYVAIGQK QSTIANVVRK LEENGALANT IVVAASASES AALQYLAPYS GCTMGEYFRD RGEDALIVYD DLSKQAVAYR QISLLLRRPP GREAYPGDVF YLHSRLLERA SRVSEEYVEK FTNGAVTGKT GSLTALPIIE TQAGDVSAFV PTNVISITDG QIFLESAMFN SGIRPAVNAG ISVSRVGGAA QTKIIKKLSG GIRTALAQYR ELAAFAQFAS DLDEATRKQL EHGQRVTELM KQKQYAPMSI AEMSLSLYAA ERGFLQDVEI AKVGSFEQAL ISYFQREHAA LLAKINEKGD FNDEIDAGIK AGIEKFKATQ TW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGC AACTCAATCC TTCCGAAATT AGTGAAATCA TCAAGGGGCG CATCGAGAAA CTCGACGTCG CCTCCCAAGC ACGAAACGAA GGCACCATCG TCAGCGTGTC CGACGGTATC GTGCGCATCT ACGGTCTGGC CGACGTCATG TACGGCGAGA TGATCGAGTT CCCGGGCGGC GTCTACGGTA TGGCGCTGAA CCTGGAGCAG GATTCCGTGG GTGCCGTGGT CCTGGGTGAA TACCAGGGCC TCGCCGAAGG CATGAACGCC AAGTGCACCG GGCGCATCCT GGAGGTTCCG GTCGGTCCGG AACTGCTGGG TCGCGTAGTC GACGCACTGG GCAATCCGAT CGACGGCAAG GGTCCGATCG ACGCCAAGGC CACCGACGCG GTCGAGAAGG TCGCTCCGGG CGTGATCTGG CGTAAGTCGG TAGACCAGCC GGTACAGACC GGCTACAAGT CGGTCGACGC CATGATCCCG GTAGGCCGTG GCCAGCGCGA GCTGATCATC GGCGACCGCC AGATCGGCAA GACCGCCCTC GCGGTCGACG CCATCATCAA CCAGAAAGAC AGCGGCATTA AGTGCGTCTA CGTGGCCATC GGCCAGAAGC AGTCCACTAT CGCCAACGTC GTACGCAAGC TGGAAGAGAA CGGTGCCCTG GCCAACACCA TCGTGGTCGC CGCCAGCGCT TCCGAGTCCG CCGCCCTGCA ATACCTGGCG CCGTACTCCG GTTGCACCAT GGGCGAATAC TTCCGCGATC GCGGTGAAGA CGCCCTGATC GTCTACGACG ACCTGTCCAA GCAGGCCGTG GCTTATCGCC AGATCTCCCT GCTGCTGCGC CGTCCGCCGG GTCGCGAAGC CTACCCGGGC GACGTGTTCT ATCTCCACAG CCGTCTGCTG GAGCGCGCTT CCCGCGTTTC CGAAGAGTAT GTCGAGAAGT TCACCAACGG CGCCGTGACC GGCAAGACCG GCTCGCTGAC CGCGCTGCCG ATCATCGAGA CCCAGGCCGG CGACGTTTCC GCGTTCGTTC CGACCAACGT GATCTCGATC ACCGACGGCC AGATCTTCCT GGAATCCGCC ATGTTCAACT CGGGTATCCG TCCGGCCGTC AACGCCGGTA TCTCGGTATC CCGCGTAGGT GGCGCGGCCC AGACCAAGAT CATCAAGAAG CTGTCCGGCG GCATCCGTAC CGCTCTGGCC CAGTACCGTG AACTGGCGGC CTTCGCCCAG TTCGCCTCGG ACCTGGACGA AGCGACCCGC AAGCAGCTGG AGCATGGTCA GCGCGTTACC GAGCTGATGA AGCAGAAGCA GTACGCGCCG ATGTCGATCG CCGAGATGTC GCTGTCCCTG TATGCCGCCG AGCGTGGCTT CCTGCAGGAC GTCGAAATCG CCAAGGTCGG TAGCTTCGAG CAGGCGTTGA TCTCCTACTT CCAGCGCGAG CATGCCGCTC TGCTGGCGAA GATCAACGAG AAAGGTGACT TCAACGACGA AATCGACGCG GGCATCAAGG CCGGCATCGA GAAGTTCAAG GCCACCCAAA CCTGGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT