SSGCID: Target Page

PsaeA.17369.a

ATP synthase subunit beta (EC 3.6.3.14) (ATP synthase F1 sector subunit beta) (F-ATPase subunit beta)

CENTER ID:	PsaeA.17369.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17369.a.B1.GE38328	full length		1	458

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5820
PGAT:	pseudomonas:PA5554
RefSeq:	NP_254241.1
UniProt:	Q9HT20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSGRIVQII GAVIDVEFPR DAVPSIYEAL KVQGVETTLE VQQQLGDGVV RSIAMGSTEG LKRGLNVDST GAAISVPVGK ATLGRIMDVL GNPIDEAGPI GEEERWGIHR EAPSYADQAG GNELLETGIK VIDLVCPFAK GGKVGLFGGA GVGKTVNMME LIRNIAIEHS GYSVFAGVGE RTREGNDFYH EMKDSNVLDK VALVYGQMNE PPGNRLRVAL TGLTMAEKFR DEGRDVLLFI DNIYRYTLAG TEVSALLGRM PSAVGYQPTL AEEMGVLQER ITSTKKGSIT SIQAVYVPAD DLTDPSPATT FAHLDATVVL SRDIASLGIY PAVDPLDSTS RQLDPLVIGQ DHYDTARGVQ YVLQRYKELK DIIAILGMDE LSEADKLLVA RARKIQRFLS QPFFVAEVFT GSPGKYVSLK DTIAGFKGIL NGDYDHLPEQ AFYMVGGIEE AVEKAKKL

NT Sequence

atgagtagcg gacgtatcgt tcaaatcatc ggcgccgtga tcgacgtgga attcccgcgc gatgccgtgc cgagcatcta tgaagccctg aaggttcagg gcgttgaaac caccctggaa gtccagcagc agctgggcga cggcgtggtt cgttccattg cgatgggttc caccgaaggc ctcaagcgtg gcctgaacgt ggacagcact ggtgctgcca tctccgtgcc ggtcggtaaa gcgaccctgg gccggatcat ggacgtactg ggtaacccga tcgacgaagc cggtcccatc ggcgaagaag agcgctgggg catccaccgc gaagctccgt cctatgccga ccaggctggc ggcaacgagc tgctggaaac cggcatcaag gtgatcgacc tggtctgccc gttcgccaag ggcggcaagg taggcctgtt cggcggcgcc ggcgtgggca agaccgtcaa catgatggaa ctgatccgta acatcgccat cgagcacagc ggttattccg tgttcgcggg tgtgggcgag cgtactcgtg agggtaacga cttctaccac gagatgaagg actccaacgt actggacaag gtagccctgg tatacggcca gatgaacgag ccgccgggca accgtctgcg cgtcgcactg accggcctga ccatggccga gaagttccgt gacgaaggcc gtgacgttct gttgttcatc gacaacatct accgttacac cctcgccggt accgaagtat ccgcactgct gggccgtatg ccgtccgcgg tgggctatca gccgaccctg gccgaagaga tgggcgttct gcaggagcgc atcacctcca ccaagaaagg ctcgatcacc tcgatccagg ccgtatacgt tcccgcggac gacctcaccg acccgtcccc ggcgaccacc ttcgcccacc tggacgcgac cgtcgtactg tcccgtgaca tcgcctcgct gggtatctac cccgcggtcg acccgctgga ctccacctct cgccagctcg atccgctggt gatcggccag gatcactacg acaccgctcg cggcgtccag tatgttctgc agcgctacaa ggagctgaag gacatcatcg cgatcctggg catggacgaa ctgtccgaag ccgacaagct gctggtggcg cgcgctcgta agatccagcg cttcctgtcc cagccgttct tcgtagcgga agtcttcacc ggctcgccgg gcaagtacgt ttccctgaag gacaccatcg ctggcttcaa gggcattctc aacggcgact acgatcacct gccggagcag gcgttctaca tggtcggcgg tatcgaagaa gcagtcgaga aagccaaaaa actgtaa

Details for PsaeA.17369.a.B1.GE38328

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	51kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	68

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatgagtag cggacgtatc gttcaaatca tcggcgccgt gatcgacgtg gaattcccgc gcgatgccgt gccgagcatc tatgaagccc tgaaggttca gggcgttgaa accaccctgg aagtccagca gcagctgggc gacggcgtgg ttcgttccat tgcgatgggt tccaccgaag gcctcaagcg tggcctgaac gtggacagca ctggtgctgc catctccgtg ccggtcggta aagcgaccct gggccggatc atggacgtac tgggtaaccc gatcgacgaa gccggtccca tcggcgaaga agagcgctgg ggcatccacc gcgaagctcc gtcctatgcc gaccaggctg gcggcaacga gctgctggaa accggcatca aggtgatcga cctggtctgc ccgttcgcca agggcggcaa ggtaggcctg ttcggcggcg ccggcgtggg caagaccgtc aacatgatgg aactgatccg taacatcgcc atcgagcaca gcggttattc cgtgttcgcg ggtgtgggcg agcgtactcg tgagggtaac gacttctacc acgagatgaa ggactccaac gtactggaca aggtagccct ggtatacggc cagatgaacg agccgccggg caaccgtctg cgcgtcgcac tgaccggcct gaccatggcc gagaagttcc gtgacgaagg ccgtgacgtt ctgttgttca tcgacaacat ctaccgttac accctcgccg gtaccgaagt atccgcactg ctgggccgta tgccgtccgc ggtgggctat cagccgaccc tggccgaaga gatgggcgtt ctgcaggagc gcatcacctc caccnagaaa ggctcgatca cctcgatcca ggccgtatac gttcccgcgg acgacctcac cgacccgtcc ccggcnacca ccnttcgccc nnnnngnann nnannnncgn actgnnc

Expected Protein Sequence

MAHHHHHHMS SGRIVQIIGA VIDVEFPRDA VPSIYEALKV QGVETTLEVQ QQLGDGVVRS IAMGSTEGLK RGLNVDSTGA AISVPVGKAT LGRIMDVLGN PIDEAGPIGE EERWGIHREA PSYADQAGGN ELLETGIKVI DLVCPFAKGG KVGLFGGAGV GKTVNMMELI RNIAIEHSGY SVFAGVGERT REGNDFYHEM KDSNVLDKVA LVYGQMNEPP GNRLRVALTG LTMAEKFRDE GRDVLLFIDN IYRYTLAGTE VSALLGRMPS AVGYQPTLAE EMGVLQERIT STKKGSITSI QAVYVPADDL TDPSPATTFA HLDATVVLSR DIASLGIYPA VDPLDSTSRQ LDPLVIGQDH YDTARGVQYV LQRYKELKDI IAILGMDELS EADKLLVARA RKIQRFLSQP FFVAEVFTGS PGKYVSLKDT IAGFKGILNG DYDHLPEQAF YMVGGIEEAV EKAKKL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta gcggacgtat cgttcaaatc atcggcgccg tgatcgacgt ggaattcccg cgcgatgccg tgccgagcat ctatgaagcc ctgaaggttc agggcgttga aaccaccctg gaagtccagc agcagctggg cgacggcgtg gttcgttcca ttgcgatggg ttccaccgaa ggcctcaagc gtggcctgaa cgtggacagc actggtgctg ccatctccgt gccggtcggt aaagcgaccc tgggccggat catggacgta ctgggtaacc cgatcgacga agccggtccc atcggcgaag aagagcgctg gggcatccac cgcgaagctc cgtcctatgc cgaccaggct ggcggcaacg agctgctgga aaccggcatc aaggtgatcg acctggtctg cccgttcgcc aagggcggca aggtaggcct gttcggcggc gccggcgtgg gcaagaccgt caacatgatg gaactgatcc gtaacatcgc catcgagcac agcggttatt ccgtgttcgc gggtgtgggc gagcgtactc gtgagggtaa cgacttctac cacgagatga aggactccaa cgtactggac aaggtagccc tggtatacgg ccagatgaac gagccgccgg gcaaccgtct gcgcgtcgca ctgaccggcc tgaccatggc cgagaagttc cgtgacgaag gccgtgacgt tctgttgttc atcgacaaca tctaccgtta caccctcgcc ggtaccgaag tatccgcact gctgggccgt atgccgtccg cggtgggcta tcagccgacc ctggccgaag agatgggcgt tctgcaggag cgcatcacct ccaccaagaa aggctcgatc acctcgatcc aggccgtata cgttcccgcg gacgacctca ccgacccgtc cccggcgacc accttcgccc acctggacgc gaccgtcgta ctgtcccgtg acatcgcctc gctgggtatc taccccgcgg tcgacccgct ggactccacc tctcgccagc tcgatccgct ggtgatcggc caggatcact acgacaccgc tcgcggcgtc cagtatgttc tgcagcgcta caaggagctg aaggacatca tcgcgatcct gggcatggac gaactgtccg aagccgacaa gctgctggtg gcgcgcgctc gtaagatcca gcgcttcctg tcccagccgt tcttcgtagc ggaagtcttc accggctcgc cgggcaagta cgtttccctg aaggacacca tcgctggctt caagggcatt ctcaacggcg actacgatca cctgccggag caggcgttct acatggtcgg cggtatcgaa gaagcagtcg agaaagccaa aaaactgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at