PsaeA.17274.a

DNA-directed RNA polymerase subunit beta-prime (RNAP subunit beta-prime) (EC 2.7.7.6) (RNA polymerase subunit beta-prime) (Transcriptase subunit beta-prime)

CENTER ID: PsaeA.17274.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.17274.a.B1.GE38322 full length 1 1399
PsaeA.17274.a.B2.GE38323 structural domain (4AYB_A) 12 844
PsaeA.17274.a.B3.GE38324 structural domain (4AYB_C) 846 1365
PsaeA.17274.a.B5.GE38326 RNA polymerase alpha subunit, domains 4-5 674 1321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4470
PGAT: pseudomonas:PA4269
RefSeq: NP_252959.1
UniProt: Q9HWC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDLLNLLKN QGQIEEFDAI RIGLASPEMI RSWSFGEVKK PETINYRTFK PERDGLFCAK IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVALAKVRRE RMGHIELASP VAHIWFLKSL PSRIGLLLDM TLRDIERVLY FESYVVIDPG MTTLEKGQLL NDEQYFEALE EFGDDFDARM GAEAVHELLN AIDLEHEIGR LREEIPQTNS ETKIKKLSKR LKLMEAFQGS GNKPEWMVLT VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPT LRLHQCGLPK KMALELFKPF IFGKLEGRGM ATTIKAAKKM VERELPEVWD VLAEVIREHP VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA RALMMSTNNI LSPANGEPII VPSQDVVMGL YYMTREAINA KGEGMAFADL QEVDRAYRSG QASLHARVKV RINEKIKGED GQLTANTRIV DTTVGRALLF QVVPAGLPFD VVNQSMKKKA ISKLINHCYR VVGLKDTVIF ADQLMYTGFA YSTISGVSIG VNDFVIPDEK ARIINAATDE VKEIESQYAS GLVTQGEKYN KVIDLWSKAN DEVSKAMMAN LSKEKVVDRE GKEVDQESFN SMYMMADSGA RGSAAQIRQL AGMRGLMAKP DGSIIETPIT ANFREGLNVL QYFISTHGAR KGLADTALKT ANSGYLTRRL VDVAQDLVVT EIDCGTEHGL LMSPHIEGGD VVEPLGERVL GRVIARDVFK PGSDEVIVPA GTLIDEKWVD FLEVMSVDEV VVRSPITCET RHGICAMCYG RDLARGHRVN IGEAVGVIAA QSIGEPGTQL TMRTFHIGGA ASRTSAADNV QVKNGGTIRL HNLKHVVRAD GALVAVSRSG ELAVADDFGR ERERYKLPYG AVISVKEGDK VDPGAIVAKW DPHTHPIVTE VDGTVAFVGM EEGITVKRQT DELTGLTNIE VMDPKDRPAA GKDIRPAVKL IDAAGKDLLL PGTDVPAQYF LPANALVNLT DGAKVSIGDV VARIPQETSK TRDITGGLPR VADLFEARRP KEPSILAEIS GTISFGKETK GKRRLVITPN DGSDPYEELI PKWRHLNVFE GEQVNRGEVI SDGPSNPHDI LRLLGVSSLA KYIVNEIQDV YRLQGVKIND KHIETILRQM LRKVEVSESG DSSFIKGDQV ELTQVLEENE QLGTEDKFPA KYERVLLGIT KASLSTESFI SAASFQETTR VLTEAAVTGK RDFLRGLKEN VVVGRLIPAG TGLAYHSERK RQRDLGKPQR VSASEAEAAL TEALNSSGN
NT Sequence
ttgaaagact tgcttaatct gttgaaaaac cagggtcaaa tcgaagagtt cgatgccatc cgtattggcc tggcttcgcc cgagatgatt cgttcctggt ctttcggcga agttaaaaag ccggaaacca tcaactaccg taccttcaag ccggagcgcg acggcctgtt ctgcgccaag atcttcggcc cggtgaagga ttacgagtgc ctgtgcggca agtacaagcg cctcaagcac cgcggtgtga tctgcgagaa gtgcggcgtg gaagtcgccc tggccaaggt gcgccgcgag cgcatgggcc acatcgagct ggcttcgccg gttgcccaca tctggttcct gaagtcgctg ccgtcccgta tcggcctgct gctggacatg accctgcgcg atatcgagcg cgtgctctat ttcgagagct acgtggtgat cgatccgggc atgaccaccc tggaaaaggg ccagctgctg aacgacgagc aatacttcga ggccctcgaa gagttcggtg acgatttcga tgctcgcatg ggcgccgaag ctgttcacga actgctcaac gccatcgacc tggagcacga gatcggccgc ctgcgcgaag agattccgca gaccaactcg gaaaccaaga tcaagaagct gtccaagcgc ctgaagctga tggaagcctt ccagggctcc ggcaacaagc ctgagtggat ggtcctgacc gtcctgccgg tgctgccgcc ggacctgcgt ccgctggttc cgctggatgg cggtcgcttc gcgacttccg acctgaacga cctgtatcgt cgggtgatca accgtaacaa ccgtctgaag cgcctgctcg acctggctgc tccggacatc atcgtgcgca acgaaaagcg catgctgcag gaagccgtcg acgccctgct cgacaacggc cgtcgcggtc gcgccatcac cggctcgaac aagcgtccgc tgaagtcgct ggccgacatg atcaagggca agcaaggtcg cttccgtcag aacctgctcg gcaagcgcgt ggactactcc ggtcgttccg tgatcaccgt gggcccgacc ctgcgcctgc accagtgcgg tctgccgaag aagatggccc tggagctgtt caagccgttc attttcggca agctggaagg tcgtggtatg gccaccacca tcaaggccgc caagaaaatg gtcgagcgcg agctgcccga ggtctgggac gttctcgccg aagtcatccg cgagcatccg gttctcctga accgtgcgcc gaccctgcac cgtctgggca tccaggcgtt cgagccggtc ctcatcgaag gcaaggcgat ccagctgcac ccgctggtct gcgccgcgta caacgccgac ttcgacggtg accagatggc cgtccacgtt ccgctgaccc tcgaggccca gctggaagcg cgcgcgctga tgatgtccac caacaacatc ctgtcgcccg ccaacggcga gccgatcatc gttccgtcgc aggacgtggt aatgggtctg tactacatga cccgtgaagc gatcaacgcg aagggcgagg gcatggcctt cgccgacctg caggaagtcg accgcgccta ccgcagcggc caggcgtccc tgcacgctcg cgtaaaagtg cgcatcaacg aaaagatcaa gggtgaagac ggccaactga ccgccaatac ccgtatcgtc gacaccaccg tcggccgcgc gctgctgttc caggtcgttc cggccggcct gccgttcgac gtggtcaacc agtcgatgaa gaagaaggcg atctccaagc tgatcaacca ctgctatcgc gtggtgggtc tgaaggacac cgtcatcttc gccgaccagc tgatgtacac cggtttcgcc tactcgacca tttccggcgt gtccatcggc gtcaacgact tcgtcatccc ggacgagaag gcgcggatca tcaacgccgc caccgacgaa gtgaaggaga tcgagagcca gtacgcctcc ggcctggtaa cccagggcga gaagtacaac aaggtgatcg acctgtggtc gaaggccaac gacgaagtgt ccaaggcgat gatggccaac ctctcgaaag agaaggtcgt cgatcgcgag ggcaaggaag tcgaccagga gtccttcaac tccatgtaca tgatggctga ctcgggtgcg cggggctccg cggcccagat ccgtcagctg gcaggtatgc gtggcctgat ggccaagccg gacggctcga tcatcgagac cccgatcacc gcgaacttcc gtgaaggcct gaacgtactc cagtacttca tctccaccca cggtgctcgt aagggtctgg cggataccgc gctgaagacc gcgaactccg gttacctgac ccgtcgtctg gtcgacgtgg cccaggatct ggtagtgacc gagatcgatt gcggtaccga gcacggcctg ctgatgtcgc cgcacatcga aggcggcgac gtggtcgaac cgctcggcga gcgcgtgctc ggccgtgtga tcgcgcgcga cgtgttcaag ccgggcagcg acgaggtcat cgtgccggcc ggcaccctga tcgacgagaa gtgggtggac ttcctcgaag tgatgagcgt cgacgaagtg gtggtgcgtt ccccgatcac ctgcgaaacc cgtcatggca tctgcgccat gtgctacggc cgcgacctgg cccgtggcca tcgcgtcaac atcggcgagg cggtcggtgt catcgctgcc cagtccatcg gtgagccggg cacccagctg accatgcgta ccttccacat cggtggtgcg gccagccgga cctccgcggc cgacaacgtc caggtgaaga acggcggcac catccgcctg cacaacctga agcatgtggt tcgtgccgac ggcgcgctgg tcgcggtttc ccgttccggc gaactggcgg ttgccgacga cttcggtcgc gagcgcgagc gctacaagct gccgtacggt gcggtgatct cggtcaagga aggtgacaag gtcgacccgg gcgctatcgt cgccaagtgg gacccgcaca cccacccgat cgtcaccgag gtggacggta ccgtggcctt cgtgggcatg gaagagggca tcaccgtcaa gcgtcagacc gacgaactga ccggcctgac caacatcgaa gtgatggatc cgaaggaccg tccggctgcc ggcaaggaca tccgtccggc cgtgaagctg attgatgccg cgggcaagga cctgctgctg ccgggtaccg acgtaccggc gcagtacttc ctgccggcca acgccctggt caacctgacc gacggcgcca aggtgagcat cggtgacgtt gtcgcgcgta tcccgcagga aacctcgaag acccgtgaca tcaccggtgg tctgccgcgc gttgccgacc tgttcgaggc tcgtcgtccg aaagagcctt cgatcctggc ggaaatcagc ggcaccatct ccttcggcaa ggagaccaag ggcaagcgcc gcctggtcat cacgccgaac gatggcagcg atccgtacga ggagctgatt ccgaagtggc gtcacctgaa cgtgttcgaa ggcgaacagg tgaaccgcgg cgaagtcatc tccgacggtc cgagcaaccc gcacgacatc ctgcgcctcc tgggcgtgag ctcgctggcg aagtacatcg tcaacgagat tcaggacgtc taccgtctgc agggcgtgaa gatcaacgac aagcacatcg agaccatcct gcgtcagatg ctgcgcaagg tcgaagtcag cgagtccggc gactccagct tcatcaaagg cgaccaggtg gaactcaccc aggtgctgga agagaacgaa cagctcggta ccgaggacaa gttcccggcc aagtacgagc gggttctgct gggtatcacc aaggcctccc tgtcgaccga gtcgttcatt tccgcggcgt cgttccagga gaccacccgc gtcctcaccg aggcggcggt caccggcaag cgcgacttcc tgcgtggtct gaaagagaac gtggtcgtgg gtcgcctgat cccggccggt accggtctgg cttaccacag cgaacgcaag cgtcagcgcg acctcggcaa gccgcagcgt gtgagtgcca gcgaggcgga agccgcactg accgaagcgc tgaactcgag cggtaactaa
Details for PsaeA.17274.a.B1.GE38322
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 155kDa
OBSERVED MW: 150kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 23
Validated AA Sequence
MAHHHHHHML KDLLNLLKNQ GQIEEFDAIR IGLASPEMIR SWSFGEVKKP ETINYRTFKP ERDGLFCAKI FGPVKDYECL CGKYKRLKHR GVICEKCGVE VALAKVRRER MGHIELASPV AHIWFLKSLP SRIGLLLDMT LRDIERVLYF ESYVVIDPGM TTLEKGQLLN DEQYFEALEE FGDDFDARMG AEAVHELLNA IDLEHEIGRL REEIPQTNSE TKIKKLSKRL KLMEAFQGSG NKPEWMVLTV LPVLPPDLRP LVPLDGGRFA TSDLNDLYRR VINRNNRLKR LLDLAAPGHH RAQRKAHAAG SRRRPARQRA VAXAPSPGSN SVR
Validated NT Sequence
cnntananna ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg aaagacttgc ttaatctgtt gaaaaaccag ggtcaaatcg aagagttcga tgccatccgt attggcctgg cttcgcccga gatgattcgt tcctggtctt tcggcgaagt taaaaagccg gaaaccatca actaccgtac cttcaagccg gagcgcgacg gcctgttctg cgccaagatc ttcggcccgg tgaaggatta cgagtgcctg tgcggcaagt acaagcgcct caagcaccgc ggtgtgatct gcgagaagtg cggcgtggaa gtcgccctgg ccaaggtgcg ccgcgagcgc atgggccaca tcgagctggc ttcgccggtt gcccacatct ggttcctgaa gtcgctgccg tcccgtatcg gcctgctgct ggacatgacc ctgcgcgata tcgagcgcgt gctctatttc gagagctacg tggtgatcga tccgggcatg accaccctgg aaaagggcca gctgctgaac gacgagcaat acttcgaggc cctcgaagag ttcggtgacg atttcgatgc tcgcatgggc gccgaagctg ttcacgaact gctcaacgcc atcgacctgg agcacgagat cggccgcctg cgcgaagaga ttccgcagac caactcggaa accaagatca agaagctgtc caagcgcctg aagctgatgg aagccttcca gggctccggc aacaagcctg agtggatggt cctgaccgtc ctgccggtgc tgccgccgga cctgcgtccg ctggttccgc tggatggcgg tcgcttcgcg acttccgacc tgaacgacct gtatcgtcgg gtgatcaacc gtaacaaccg tctgaagcgc ctgctcgacc tggctgctcc cggacatcat cgtgcgcaac gaaaagcgca tgctgcagga agccgtcgac gccctgctcg acaacgggcc gtcgcngncg cgccatcacc nggctcgaac agcgtccgct gantcgctgg ncgacatgat cannnagcaa gncgcttccg tcaganngct cggcagcnnn gngnctactc nncntnnnga tcacnnnggn cngancnnnc nncnnnnnng nctgccnann nnangnnnnn nnnnnnnnnn nanntcattt tnngnnaagc ntnnngnaa
Expected Protein Sequence
MAHHHHHHMK DLLNLLKNQG QIEEFDAIRI GLASPEMIRS WSFGEVKKPE TINYRTFKPE RDGLFCAKIF GPVKDYECLC GKYKRLKHRG VICEKCGVEV ALAKVRRERM GHIELASPVA HIWFLKSLPS RIGLLLDMTL RDIERVLYFE SYVVIDPGMT TLEKGQLLND EQYFEALEEF GDDFDARMGA EAVHELLNAI DLEHEIGRLR EEIPQTNSET KIKKLSKRLK LMEAFQGSGN KPEWMVLTVL PVLPPDLRPL VPLDGGRFAT SDLNDLYRRV INRNNRLKRL LDLAAPDIIV RNEKRMLQEA VDALLDNGRR GRAITGSNKR PLKSLADMIK GKQGRFRQNL LGKRVDYSGR SVITVGPTLR LHQCGLPKKM ALELFKPFIF GKLEGRGMAT TIKAAKKMVE RELPEVWDVL AEVIREHPVL LNRAPTLHRL GIQAFEPVLI EGKAIQLHPL VCAAYNADFD GDQMAVHVPL TLEAQLEARA LMMSTNNILS PANGEPIIVP SQDVVMGLYY MTREAINAKG EGMAFADLQE VDRAYRSGQA SLHARVKVRI NEKIKGEDGQ LTANTRIVDT TVGRALLFQV VPAGLPFDVV NQSMKKKAIS KLINHCYRVV GLKDTVIFAD QLMYTGFAYS TISGVSIGVN DFVIPDEKAR IINAATDEVK EIESQYASGL VTQGEKYNKV IDLWSKANDE VSKAMMANLS KEKVVDREGK EVDQESFNSM YMMADSGARG SAAQIRQLAG MRGLMAKPDG SIIETPITAN FREGLNVLQY FISTHGARKG LADTALKTAN SGYLTRRLVD VAQDLVVTEI DCGTEHGLLM SPHIEGGDVV EPLGERVLGR VIARDVFKPG SDEVIVPAGT LIDEKWVDFL EVMSVDEVVV RSPITCETRH GICAMCYGRD LARGHRVNIG EAVGVIAAQS IGEPGTQLTM RTFHIGGAAS RTSAADNVQV KNGGTIRLHN LKHVVRADGA LVAVSRSGEL AVADDFGRER ERYKLPYGAV ISVKEGDKVD PGAIVAKWDP HTHPIVTEVD GTVAFVGMEE GITVKRQTDE LTGLTNIEVM DPKDRPAAGK DIRPAVKLID AAGKDLLLPG TDVPAQYFLP ANALVNLTDG AKVSIGDVVA RIPQETSKTR DITGGLPRVA DLFEARRPKE PSILAEISGT ISFGKETKGK RRLVITPNDG SDPYEELIPK WRHLNVFEGE QVNRGEVISD GPSNPHDILR LLGVSSLAKY IVNEIQDVYR LQGVKINDKH IETILRQMLR KVEVSESGDS SFIKGDQVEL TQVLEENEQL GTEDKFPAKY ERVLLGITKA SLSTESFISA ASFQETTRVL TEAAVTGKRD FLRGLKENVV VGRLIPAGTG LAYHSERKRQ RDLGKPQRVS ASEAEAALTE ALNSSGN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaag acttgcttaa tctgttgaaa aaccagggtc aaatcgaaga gttcgatgcc atccgtattg gcctggcttc gcccgagatg attcgttcct ggtctttcgg cgaagttaaa aagccggaaa ccatcaacta ccgtaccttc aagccggagc gcgacggcct gttctgcgcc aagatcttcg gcccggtgaa ggattacgag tgcctgtgcg gcaagtacaa gcgcctcaag caccgcggtg tgatctgcga gaagtgcggc gtggaagtcg ccctggccaa ggtgcgccgc gagcgcatgg gccacatcga gctggcttcg ccggttgccc acatctggtt cctgaagtcg ctgccgtccc gtatcggcct gctgctggac atgaccctgc gcgatatcga gcgcgtgctc tatttcgaga gctacgtggt gatcgatccg ggcatgacca ccctggaaaa gggccagctg ctgaacgacg agcaatactt cgaggccctc gaagagttcg gtgacgattt cgatgctcgc atgggcgccg aagctgttca cgaactgctc aacgccatcg acctggagca cgagatcggc cgcctgcgcg aagagattcc gcagaccaac tcggaaacca agatcaagaa gctgtccaag cgcctgaagc tgatggaagc cttccagggc tccggcaaca agcctgagtg gatggtcctg accgtcctgc cggtgctgcc gccggacctg cgtccgctgg ttccgctgga tggcggtcgc ttcgcgactt ccgacctgaa cgacctgtat cgtcgggtga tcaaccgtaa caaccgtctg aagcgcctgc tcgacctggc tgctccggac atcatcgtgc gcaacgaaaa gcgcatgctg caggaagccg tcgacgccct gctcgacaac ggccgtcgcg gtcgcgccat caccggctcg aacaagcgtc cgctgaagtc gctggccgac atgatcaagg gcaagcaagg tcgcttccgt cagaacctgc tcggcaagcg cgtggactac tccggtcgtt ccgtgatcac cgtgggcccg accctgcgcc tgcaccagtg cggtctgccg aagaagatgg ccctggagct gttcaagccg ttcattttcg gcaagctgga aggtcgtggt atggccacca ccatcaaggc cgccaagaaa atggtcgagc gcgagctgcc cgaggtctgg gacgttctcg ccgaagtcat ccgcgagcat ccggttctcc tgaaccgtgc gccgaccctg caccgtctgg gcatccaggc gttcgagccg gtcctcatcg aaggcaaggc gatccagctg cacccgctgg tctgcgccgc gtacaacgcc gacttcgacg gtgaccagat ggccgtccac gttccgctga ccctcgaggc ccagctggaa gcgcgcgcgc tgatgatgtc caccaacaac atcctgtcgc ccgccaacgg cgagccgatc atcgttccgt cgcaggacgt ggtaatgggt ctgtactaca tgacccgtga agcgatcaac gcgaagggcg agggcatggc cttcgccgac ctgcaggaag tcgaccgcgc ctaccgcagc ggccaggcgt ccctgcacgc tcgcgtaaaa gtgcgcatca acgaaaagat caagggtgaa gacggccaac tgaccgccaa tacccgtatc gtcgacacca ccgtcggccg cgcgctgctg ttccaggtcg ttccggccgg cctgccgttc gacgtggtca accagtcgat gaagaagaag gcgatctcca agctgatcaa ccactgctat cgcgtggtgg gtctgaagga caccgtcatc ttcgccgacc agctgatgta caccggtttc gcctactcga ccatttccgg cgtgtccatc ggcgtcaacg acttcgtcat cccggacgag aaggcgcgga tcatcaacgc cgccaccgac gaagtgaagg agatcgagag ccagtacgcc tccggcctgg taacccaggg cgagaagtac aacaaggtga tcgacctgtg gtcgaaggcc aacgacgaag tgtccaaggc gatgatggcc aacctctcga aagagaaggt cgtcgatcgc gagggcaagg aagtcgacca ggagtccttc aactccatgt acatgatggc tgactcgggt gcgcggggct ccgcggccca gatccgtcag ctggcaggta tgcgtggcct gatggccaag ccggacggct cgatcatcga gaccccgatc accgcgaact tccgtgaagg cctgaacgta ctccagtact tcatctccac ccacggtgct cgtaagggtc tggcggatac cgcgctgaag accgcgaact ccggttacct gacccgtcgt ctggtcgacg tggcccagga tctggtagtg accgagatcg attgcggtac cgagcacggc ctgctgatgt cgccgcacat cgaaggcggc gacgtggtcg aaccgctcgg cgagcgcgtg ctcggccgtg tgatcgcgcg cgacgtgttc aagccgggca gcgacgaggt catcgtgccg gccggcaccc tgatcgacga gaagtgggtg gacttcctcg aagtgatgag cgtcgacgaa gtggtggtgc gttccccgat cacctgcgaa acccgtcatg gcatctgcgc catgtgctac ggccgcgacc tggcccgtgg ccatcgcgtc aacatcggcg aggcggtcgg tgtcatcgct gcccagtcca tcggtgagcc gggcacccag ctgaccatgc gtaccttcca catcggtggt gcggccagcc ggacctccgc ggccgacaac gtccaggtga agaacggcgg caccatccgc ctgcacaacc tgaagcatgt ggttcgtgcc gacggcgcgc tggtcgcggt ttcccgttcc ggcgaactgg cggttgccga cgacttcggt cgcgagcgcg agcgctacaa gctgccgtac ggtgcggtga tctcggtcaa ggaaggtgac aaggtcgacc cgggcgctat cgtcgccaag tgggacccgc acacccaccc gatcgtcacc gaggtggacg gtaccgtggc cttcgtgggc atggaagagg gcatcaccgt caagcgtcag accgacgaac tgaccggcct gaccaacatc gaagtgatgg atccgaagga ccgtccggct gccggcaagg acatccgtcc ggccgtgaag ctgattgatg ccgcgggcaa ggacctgctg ctgccgggta ccgacgtacc ggcgcagtac ttcctgccgg ccaacgccct ggtcaacctg accgacggcg ccaaggtgag catcggtgac gttgtcgcgc gtatcccgca ggaaacctcg aagacccgtg acatcaccgg tggtctgccg cgcgttgccg acctgttcga ggctcgtcgt ccgaaagagc cttcgatcct ggcggaaatc agcggcacca tctccttcgg caaggagacc aagggcaagc gccgcctggt catcacgccg aacgatggca gcgatccgta cgaggagctg attccgaagt ggcgtcacct gaacgtgttc gaaggcgaac aggtgaaccg cggcgaagtc atctccgacg gtccgagcaa cccgcacgac atcctgcgcc tcctgggcgt gagctcgctg gcgaagtaca tcgtcaacga gattcaggac gtctaccgtc tgcagggcgt gaagatcaac gacaagcaca tcgagaccat cctgcgtcag atgctgcgca aggtcgaagt cagcgagtcc ggcgactcca gcttcatcaa aggcgaccag gtggaactca cccaggtgct ggaagagaac gaacagctcg gtaccgagga caagttcccg gccaagtacg agcgggttct gctgggtatc accaaggcct ccctgtcgac cgagtcgttc atttccgcgg cgtcgttcca ggagaccacc cgcgtcctca ccgaggcggc ggtcaccggc aagcgcgact tcctgcgtgg tctgaaagag aacgtggtcg tgggtcgcct gatcccggcc ggtaccggtc tggcttacca cagcgaacgc aagcgtcagc gcgacctcgg caagccgcag cgtgtgagtg ccagcgaggc ggaagccgca ctgaccgaag cgctgaactc gagcggtaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for PsaeA.17274.a.B2.GE38323
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 94kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 38
Validated AA Sequence
MAHHHHHHMG QIEEFDAIRI GLASPEMIRS WSFGEVKKPE TINYRTFKPE RDGLFCAKIF GPVKDYECLC GKYKRLKHRG VICEKCGVEV ALAKVRRERM GHIELASPVA HIWFLKSLPS RIGLLLDMTL RDIERVLYFE SYVVIDPGMT TLEKGQLLND EQYFEALEEF GDDFDARMGA EAVHELLNAI DLEHEIGRLR EEIPQTNSET KIKKLSKRLK LMEAFQGSGN KPEWMVLTVL PVLPPDLRPL VPLDGGRFAT SDLNDLYRRV INRNNRLKRL LDLAAPDIIV RNESACCRSR RRPARHGRRG RASRLDSVAX SLAT
Validated NT Sequence
gannnnnnta nanaattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgggtcaaat cgaagagttc gatgccatcc gtattggcct ggcttcgccc gagatgattc gttcctggtc tttcggcgaa gttaaaaagc cggaaaccat caactaccgt accttcaagc cggagcgcga cggcctgttc tgcgccaaga tcttcggccc ggtgaaggat tacgagtgcc tgtgcggcaa gtacaagcgc ctcaagcacc gcggtgtgat ctgcgagaag tgcggcgtgg aagtcgccct ggccaaggtg cgccgcgagc gcatgggcca catcgagctg gcttcgccgg ttgcccacat ctggttcctg aagtcgctgc cgtcccgtat cggcctgctg ctggacatga ccctgcgcga tatcgagcgc gtgctctatt tcgagagcta cgtggtgatc gatccgggca tgaccaccct ggaaaagggc cagctgctga acgacgagca atacttcgag gccctcgaag agttcggtga cgatttcgat gctcgcatgg gcgccgaagc tgttcacgaa ctgctcaacg ccatcgacct ggagcacgag atcggccgcc tgcgcgaaga gattccgcag accaactcgg aaaccaagat caagaagctg tccaagcgcc tgaagctgat ggaagccttc cagggctccg gcaacaagcc tgagtggatg gtcctgaccg tcctgccggt gctgccgccg gacctgcgtc cgctggttcc gctggatggc ggtcgcttcg cgacttccga cctgaacgac ctgtatcgtc gggtgatcaa ccgtaacaac cgtctgaagc gcctgctcga cctggctgct ccggacatca tcgtgcgcaa cgaaagcgca tgctgcagaa gccgtcgacg ccctgctcga cacggccgtc gcggtcgcgc atcacggctc gacagcgtcg ctgantcgct ggcgacatga tcanggcagc agtcgcttcg tcaganngct cgnnagcgcg ngnctactcg gtcgttcgtg atcncgngga ncnnnnnngn ncnnnnnngn ntgcgannaa natnnntngn anntnttagc nnnatttnnn anntnnnnnn nnnnnncncn cnnnnangnc gnncaaannn nnnaanncnn aa
Expected Protein Sequence
MAHHHHHHGQ IEEFDAIRIG LASPEMIRSW SFGEVKKPET INYRTFKPER DGLFCAKIFG PVKDYECLCG KYKRLKHRGV ICEKCGVEVA LAKVRRERMG HIELASPVAH IWFLKSLPSR IGLLLDMTLR DIERVLYFES YVVIDPGMTT LEKGQLLNDE QYFEALEEFG DDFDARMGAE AVHELLNAID LEHEIGRLRE EIPQTNSETK IKKLSKRLKL MEAFQGSGNK PEWMVLTVLP VLPPDLRPLV PLDGGRFATS DLNDLYRRVI NRNNRLKRLL DLAAPDIIVR NEKRMLQEAV DALLDNGRRG RAITGSNKRP LKSLADMIKG KQGRFRQNLL GKRVDYSGRS VITVGPTLRL HQCGLPKKMA LELFKPFIFG KLEGRGMATT IKAAKKMVER ELPEVWDVLA EVIREHPVLL NRAPTLHRLG IQAFEPVLIE GKAIQLHPLV CAAYNADFDG DQMAVHVPLT LEAQLEARAL MMSTNNILSP ANGEPIIVPS QDVVMGLYYM TREAINAKGE GMAFADLQEV DRAYRSGQAS LHARVKVRIN EKIKGEDGQL TANTRIVDTT VGRALLFQVV PAGLPFDVVN QSMKKKAISK LINHCYRVVG LKDTVIFADQ LMYTGFAYST ISGVSIGVND FVIPDEKARI INAATDEVKE IESQYASGLV TQGEKYNKVI DLWSKANDEV SKAMMANLSK EKVVDREGKE VDQESFNSMY MMADSGARGS AAQIRQLAGM RGLMAKPDGS IIETPITANF REGLNVLQYF ISTHGARKGL ADTALKTANS GYLTRRLVDV AQDLVVTEID CGTEHGLLMS PHIEGGDVVE PLGERVLGRV I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtcaaa tcgaagagtt cgatgccatc cgtattggcc tggcttcgcc cgagatgatt cgttcctggt ctttcggcga agttaaaaag ccggaaacca tcaactaccg taccttcaag ccggagcgcg acggcctgtt ctgcgccaag atcttcggcc cggtgaagga ttacgagtgc ctgtgcggca agtacaagcg cctcaagcac cgcggtgtga tctgcgagaa gtgcggcgtg gaagtcgccc tggccaaggt gcgccgcgag cgcatgggcc acatcgagct ggcttcgccg gttgcccaca tctggttcct gaagtcgctg ccgtcccgta tcggcctgct gctggacatg accctgcgcg atatcgagcg cgtgctctat ttcgagagct acgtggtgat cgatccgggc atgaccaccc tggaaaaggg ccagctgctg aacgacgagc aatacttcga ggccctcgaa gagttcggtg acgatttcga tgctcgcatg ggcgccgaag ctgttcacga actgctcaac gccatcgacc tggagcacga gatcggccgc ctgcgcgaag agattccgca gaccaactcg gaaaccaaga tcaagaagct gtccaagcgc ctgaagctga tggaagcctt ccagggctcc ggcaacaagc ctgagtggat ggtcctgacc gtcctgccgg tgctgccgcc ggacctgcgt ccgctggttc cgctggatgg cggtcgcttc gcgacttccg acctgaacga cctgtatcgt cgggtgatca accgtaacaa ccgtctgaag cgcctgctcg acctggctgc tccggacatc atcgtgcgca acgaaaagcg catgctgcag gaagccgtcg acgccctgct cgacaacggc cgtcgcggtc gcgccatcac cggctcgaac aagcgtccgc tgaagtcgct ggccgacatg atcaagggca agcaaggtcg cttccgtcag aacctgctcg gcaagcgcgt ggactactcc ggtcgttccg tgatcaccgt gggcccgacc ctgcgcctgc accagtgcgg tctgccgaag aagatggccc tggagctgtt caagccgttc attttcggca agctggaagg tcgtggtatg gccaccacca tcaaggccgc caagaaaatg gtcgagcgcg agctgcccga ggtctgggac gttctcgccg aagtcatccg cgagcatccg gttctcctga accgtgcgcc gaccctgcac cgtctgggca tccaggcgtt cgagccggtc ctcatcgaag gcaaggcgat ccagctgcac ccgctggtct gcgccgcgta caacgccgac ttcgacggtg accagatggc cgtccacgtt ccgctgaccc tcgaggccca gctggaagcg cgcgcgctga tgatgtccac caacaacatc ctgtcgcccg ccaacggcga gccgatcatc gttccgtcgc aggacgtggt aatgggtctg tactacatga cccgtgaagc gatcaacgcg aagggcgagg gcatggcctt cgccgacctg caggaagtcg accgcgccta ccgcagcggc caggcgtccc tgcacgctcg cgtaaaagtg cgcatcaacg aaaagatcaa gggtgaagac ggccaactga ccgccaatac ccgtatcgtc gacaccaccg tcggccgcgc gctgctgttc caggtcgttc cggccggcct gccgttcgac gtggtcaacc agtcgatgaa gaagaaggcg atctccaagc tgatcaacca ctgctatcgc gtggtgggtc tgaaggacac cgtcatcttc gccgaccagc tgatgtacac cggtttcgcc tactcgacca tttccggcgt gtccatcggc gtcaacgact tcgtcatccc ggacgagaag gcgcggatca tcaacgccgc caccgacgaa gtgaaggaga tcgagagcca gtacgcctcc ggcctggtaa cccagggcga gaagtacaac aaggtgatcg acctgtggtc gaaggccaac gacgaagtgt ccaaggcgat gatggccaac ctctcgaaag agaaggtcgt cgatcgcgag ggcaaggaag tcgaccagga gtccttcaac tccatgtaca tgatggctga ctcgggtgcg cggggctccg cggcccagat ccgtcagctg gcaggtatgc gtggcctgat ggccaagccg gacggctcga tcatcgagac cccgatcacc gcgaacttcc gtgaaggcct gaacgtactc cagtacttca tctccaccca cggtgctcgt aagggtctgg cggataccgc gctgaagacc gcgaactccg gttacctgac ccgtcgtctg gtcgacgtgg cccaggatct ggtagtgacc gagatcgatt gcggtaccga gcacggcctg ctgatgtcgc cgcacatcga aggcggcgac gtggtcgaac cgctcggcga gcgcgtgctc ggccgtgtga tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PsaeA.17274.a.B3.GE38324
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 58kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 21
PERCENT COVERAGE: 56
Validated AA Sequence
MSRVFEVXXG IRATTSPMLT XGAVGQVDQG VGRQEVLRRY VGTRQQQVLA RGINQLHGRT DVLAGSRTVL RIHHFDVGQA GQFVGLTLDG DALFHAHEGH GTVHLGDDRV GVRVPLGDDS ARVDLVTFLD RDHRTVRQLV ALALATEVVG NRQFAGTGNR DQRAVGTNHM LQVVQADGAA VLHLDVVGRG GPAGRTTDVE GTHGQLGARL TDGLGSDDTD RLADVDAMAT GQVAAVAHGA DAMTGFAGDR GTHHHFVDAH HFEEVHPLLV DQGAGRHDDL VAARLEHVAH MVVVVVS
Validated NT Sequence
ggctcaccac caccaccacc atatgcgcga cgtgttcaag ccgggcagcg acgaggtcat cgtgccggcc ggcaccctga tcgacgagaa gtgggtggac ttcctcgaag tgatgagcgt cgacgaagtg gtggtgcgtt ccccgatcac ctgcgaaacc cgtcatggca tctgcgccat gtgctacggc cgcgacctgg cccgtggcca tcgcgtcaac atcggcgagg cggtcggtgt catcgctgcc cagtccatcg gtgagccggg cacccagctg accatgcgta ccttccacat cggtggtgcg gccagccgga cctccgcggc cgacaacgtc caggtgaaga acggcggcac catccgcctg cacaacctga agcatgtggt tcgtgccgac ggcgcgctgg tcgcggtttc ccgttccggc gaactggcgg ttgccgacga cttcggtcgc gagcgcgagc gctacaagct gccgtacggt gcggtgatct cggtcaagga aggtgacaag gtcgacccgg gcgctatcgt cgccaagtgg gacccgcaca cccacccgat cgtcaccgag gtggacggta ccgtggcctt cgtgggcatg gaagagggca tcaccgtcaa gcgtcagacc gacgaactga ccggcctgac caacatcgaa gtgatggatc cgaaggaccg tccggctgcc ggcaaggaca tccgtccggc cgtgaagctg attgatgccg cgggcaagga cctgctgctg ccgggtaccg acgtaccggc gcagtacttc ctgccggcca acgccctggt caacctgacc gacggcgccn anggtgagca tcggtgacgt tgtcgcgcgt atcccgcngn aaacctcgaa nacccgtgac atcaccggtg gtctgccgcg cgttgccgac ctgttcgagn ctcgtcgtnn gaaanannnt tcgatccnng nnngaantca gcggcnnnnt c
Expected Protein Sequence
MAHHHHHHRD VFKPGSDEVI VPAGTLIDEK WVDFLEVMSV DEVVVRSPIT CETRHGICAM CYGRDLARGH RVNIGEAVGV IAAQSIGEPG TQLTMRTFHI GGAASRTSAA DNVQVKNGGT IRLHNLKHVV RADGALVAVS RSGELAVADD FGRERERYKL PYGAVISVKE GDKVDPGAIV AKWDPHTHPI VTEVDGTVAF VGMEEGITVK RQTDELTGLT NIEVMDPKDR PAAGKDIRPA VKLIDAAGKD LLLPGTDVPA QYFLPANALV NLTDGAKVSI GDVVARIPQE TSKTRDITGG LPRVADLFEA RRPKEPSILA EISGTISFGK ETKGKRRLVI TPNDGSDPYE ELIPKWRHLN VFEGEQVNRG EVISDGPSNP HDILRLLGVS SLAKYIVNEI QDVYRLQGVK INDKHIETIL RQMLRKVEVS ESGDSSFIKG DQVELTQVLE ENEQLGTEDK FPAKYERVLL GITKASLSTE SFISAASFQE TTRVLTEAAV TGKRDFLRGL KENVVVGRLI PAGTGLAY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgacg tgttcaagcc gggcagcgac gaggtcatcg tgccggccgg caccctgatc gacgagaagt gggtggactt cctcgaagtg atgagcgtcg acgaagtggt ggtgcgttcc ccgatcacct gcgaaacccg tcatggcatc tgcgccatgt gctacggccg cgacctggcc cgtggccatc gcgtcaacat cggcgaggcg gtcggtgtca tcgctgccca gtccatcggt gagccgggca cccagctgac catgcgtacc ttccacatcg gtggtgcggc cagccggacc tccgcggccg acaacgtcca ggtgaagaac ggcggcacca tccgcctgca caacctgaag catgtggttc gtgccgacgg cgcgctggtc gcggtttccc gttccggcga actggcggtt gccgacgact tcggtcgcga gcgcgagcgc tacaagctgc cgtacggtgc ggtgatctcg gtcaaggaag gtgacaaggt cgacccgggc gctatcgtcg ccaagtggga cccgcacacc cacccgatcg tcaccgaggt ggacggtacc gtggccttcg tgggcatgga agagggcatc accgtcaagc gtcagaccga cgaactgacc ggcctgacca acatcgaagt gatggatccg aaggaccgtc cggctgccgg caaggacatc cgtccggccg tgaagctgat tgatgccgcg ggcaaggacc tgctgctgcc gggtaccgac gtaccggcgc agtacttcct gccggccaac gccctggtca acctgaccga cggcgccaag gtgagcatcg gtgacgttgt cgcgcgtatc ccgcaggaaa cctcgaagac ccgtgacatc accggtggtc tgccgcgcgt tgccgacctg ttcgaggctc gtcgtccgaa agagccttcg atcctggcgg aaatcagcgg caccatctcc ttcggcaagg agaccaaggg caagcgccgc ctggtcatca cgccgaacga tggcagcgat ccgtacgagg agctgattcc gaagtggcgt cacctgaacg tgttcgaagg cgaacaggtg aaccgcggcg aagtcatctc cgacggtccg agcaacccgc acgacatcct gcgcctcctg ggcgtgagct cgctggcgaa gtacatcgtc aacgagattc aggacgtcta ccgtctgcag ggcgtgaaga tcaacgacaa gcacatcgag accatcctgc gtcagatgct gcgcaaggtc gaagtcagcg agtccggcga ctccagcttc atcaaaggcg accaggtgga actcacccag gtgctggaag agaacgaaca gctcggtacc gaggacaagt tcccggccaa gtacgagcgg gttctgctgg gtatcaccaa ggcctccctg tcgaccgagt cgttcatttc cgcggcgtcg ttccaggaga ccacccgcgt cctcaccgag gcggcggtca ccggcaagcg cgacttcctg cgtggtctga aagagaacgt ggtcgtgggt cgcctgatcc cggccggtac cggtctggct tactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.17274.a.B5.GE38326
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 48
Validated AA Sequence
MTQGEKYNKV IDLWSKANDE VSKAMMANLS KEKVVDREGK EVDQESFNSM YMMADSGARG SAAQIRQLAG MRGLMAKPDG SIIETPITAN FREGLNVLQY FISTHGARKG LADTALKTAN SGYLTRRLVD VAQDLVVTEI DCGTEHGLLM SPHIEGGDVV EPLGERVLGR VIARDVFKPG SDEVIVPAGT LIDEKWVDFL EVMSVDEVVV RSPITCETRH GICAMCYGRD LARGHRVNIG EAVGVIAAQS IGEPGTQLTM RTFHIGGAAS RTSAADNVQV KNGGTIRLHN XKHVVRAXXA LVXXFPXXXT GXCRXLXXR
Validated NT Sequence
ggctcaccac caccaccacc atatgaccca gggcgagaag tacaacaagg tgatcgacct gtggtcgaag gccaacgacg aagtgtccaa ggcgatgatg gccaacctct cgaaagagaa ggtcgtcgat cgcgagggca aggaagtcga ccaggagtcc ttcaactcca tgtacatgat ggctgactcg ggtgcgcggg gctccgcggc ccagatccgt cagctggcag gtatgcgtgg cctgatggcc aagccggacg gctcgatcat cgagaccccg atcaccgcga acttccgtga aggcctgaac gtactccagt acttcatctc cacccacggt gctcgtaagg gtctggcgga taccgcgctg aagaccgcga actccggtta cctgacccgt cgtctggtcg acgtggccca ggatctggta gtgaccgaga tcgattgcgg taccgagcac ggcctgctga tgtcgccgca catcgaaggc ggcgacgtgg tcgaaccgct cggcgagcgc gtgctcggcc gtgtgatcgc gcgcgacgtg ttcaagccgg gcagcgacga ggtcatcgtg ccggccggca ccctgatcga cgagaagtgg gtggacttcc tcgaagtgat gagcgtcgac gaagtggtgg tgcgttcccc gatcacctgc gaaacccgtc atggcatctg cgccatgtgc tacggccgcg acctggcccg tggccatcgc gtcaacatcg gcgaggcggt cggtgtcatc gctgcccagt ccatcggtga gccgggcacc cagctgacca tgcgtacctt ccacatcggt ggtgcggcca gccggacctc cgcggccgac aacgtccagg tgaagaacgg cggcaccatc cgcctgcaca accngaagca tgtggttcgt gccgannncg cgctggtcgn ngntttcccg tnnnnnnnaa ctggnngttg ccgannactt nnnncgcgag c
Expected Protein Sequence
MAHHHHHHTQ GEKYNKVIDL WSKANDEVSK AMMANLSKEK VVDREGKEVD QESFNSMYMM ADSGARGSAA QIRQLAGMRG LMAKPDGSII ETPITANFRE GLNVLQYFIS THGARKGLAD TALKTANSGY LTRRLVDVAQ DLVVTEIDCG TEHGLLMSPH IEGGDVVEPL GERVLGRVIA RDVFKPGSDE VIVPAGTLID EKWVDFLEVM SVDEVVVRSP ITCETRHGIC AMCYGRDLAR GHRVNIGEAV GVIAAQSIGE PGTQLTMRTF HIGGAASRTS AADNVQVKNG GTIRLHNLKH VVRADGALVA VSRSGELAVA DDFGRERERY KLPYGAVISV KEGDKVDPGA IVAKWDPHTH PIVTEVDGTV AFVGMEEGIT VKRQTDELTG LTNIEVMDPK DRPAAGKDIR PAVKLIDAAG KDLLLPGTDV PAQYFLPANA LVNLTDGAKV SIGDVVARIP QETSKTRDIT GGLPRVADLF EARRPKEPSI LAEISGTISF GKETKGKRRL VITPNDGSDP YEELIPKWRH LNVFEGEQVN RGEVISDGPS NPHDILRLLG VSSLAKYIVN EIQDVYRLQG VKINDKHIET ILRQMLRKVE VSESGDSSFI KGDQVELTQV LEENEQLGTE DKFPAKYERV LLGITKASLS TESFIS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacccagg gcgagaagta caacaaggtg atcgacctgt ggtcgaaggc caacgacgaa gtgtccaagg cgatgatggc caacctctcg aaagagaagg tcgtcgatcg cgagggcaag gaagtcgacc aggagtcctt caactccatg tacatgatgg ctgactcggg tgcgcggggc tccgcggccc agatccgtca gctggcaggt atgcgtggcc tgatggccaa gccggacggc tcgatcatcg agaccccgat caccgcgaac ttccgtgaag gcctgaacgt actccagtac ttcatctcca cccacggtgc tcgtaagggt ctggcggata ccgcgctgaa gaccgcgaac tccggttacc tgacccgtcg tctggtcgac gtggcccagg atctggtagt gaccgagatc gattgcggta ccgagcacgg cctgctgatg tcgccgcaca tcgaaggcgg cgacgtggtc gaaccgctcg gcgagcgcgt gctcggccgt gtgatcgcgc gcgacgtgtt caagccgggc agcgacgagg tcatcgtgcc ggccggcacc ctgatcgacg agaagtgggt ggacttcctc gaagtgatga gcgtcgacga agtggtggtg cgttccccga tcacctgcga aacccgtcat ggcatctgcg ccatgtgcta cggccgcgac ctggcccgtg gccatcgcgt caacatcggc gaggcggtcg gtgtcatcgc tgcccagtcc atcggtgagc cgggcaccca gctgaccatg cgtaccttcc acatcggtgg tgcggccagc cggacctccg cggccgacaa cgtccaggtg aagaacggcg gcaccatccg cctgcacaac ctgaagcatg tggttcgtgc cgacggcgcg ctggtcgcgg tttcccgttc cggcgaactg gcggttgccg acgacttcgg tcgcgagcgc gagcgctaca agctgccgta cggtgcggtg atctcggtca aggaaggtga caaggtcgac ccgggcgcta tcgtcgccaa gtgggacccg cacacccacc cgatcgtcac cgaggtggac ggtaccgtgg ccttcgtggg catggaagag ggcatcaccg tcaagcgtca gaccgacgaa ctgaccggcc tgaccaacat cgaagtgatg gatccgaagg accgtccggc tgccggcaag gacatccgtc cggccgtgaa gctgattgat gccgcgggca aggacctgct gctgccgggt accgacgtac cggcgcagta cttcctgccg gccaacgccc tggtcaacct gaccgacggc gccaaggtga gcatcggtga cgttgtcgcg cgtatcccgc aggaaacctc gaagacccgt gacatcaccg gtggtctgcc gcgcgttgcc gacctgttcg aggctcgtcg tccgaaagag ccttcgatcc tggcggaaat cagcggcacc atctccttcg gcaaggagac caagggcaag cgccgcctgg tcatcacgcc gaacgatggc agcgatccgt acgaggagct gattccgaag tggcgtcacc tgaacgtgtt cgaaggcgaa caggtgaacc gcggcgaagt catctccgac ggtccgagca acccgcacga catcctgcgc ctcctgggcg tgagctcgct ggcgaagtac atcgtcaacg agattcagga cgtctaccgt ctgcagggcg tgaagatcaa cgacaagcac atcgagacca tcctgcgtca gatgctgcgc aaggtcgaag tcagcgagtc cggcgactcc agcttcatca aaggcgacca ggtggaactc acccaggtgc tggaagagaa cgaacagctc ggtaccgagg acaagttccc ggccaagtac gagcgggttc tgctgggtat caccaaggcc tccctgtcga ccgagtcgtt catttcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at