SSGCID: Target Page

PsaeA.17267.a

Chaperone SurA (Peptidyl-prolyl cis-trans isomerase SurA) (PPIase SurA) (EC 5.2.1.8) (Rotamase SurA)

CENTER ID:	PsaeA.17267.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17267.a.B2.GE38321	mature protein		13	417

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17267.a.B2.PS02183	mature protein		13	417

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.630
PGAT:	pseudomonas:PA0594
RefSeq:	NP_249285.1
UniProt:	Q9I5U3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAALLCSFA HAEVVPLDRV VAIVDNDVIM QSQLDQRLRE VHQTLLKRGA PLPPEHVLTQ QVLERLIIEN IQQQIGDRSG IRISDEELNQ AMGTIAQRNG MSLEQFQTAL TRDGLSYADA REQVRREMVI SRVRQRRVAE RIQVSEQEVK NFLASDMGKI QLSEEYRLAN ILIPVPEAAS SDVIQAAARQ AQELYQQLKQ GADFGQLAIS RSAGDNALEG GEIGWRKAAQ LPQPFDSMIG SLAVGDVTEP VRTPGGFIIL KLEEKRGGSK MVRDEVHVRH ILLKPSEIRS EAETEKLAQK LYERIQSGED FGELAKSFSE DPGSALNGGD LNWIDPEALV PEFRQVMNDT PQGELSKPFR SQFGWHILQV LGRRATDSSE KFREQQAVSV LRNRKYDEEL QAWLRQIRDE AYVEIKQ

NT Sequence

TTGGGCGCAG CACTCCTTTG CTCCTTCGCG CATGCCGAAG TGGTACCACT GGACCGGGTC GTCGCCATCG TCGACAACGA TGTGATCATG CAGAGCCAGC TCGACCAGCG CCTGCGTGAA GTGCACCAGA CGCTGCTCAA GCGCGGTGCG CCGCTGCCGC CCGAGCACGT GCTGACCCAG CAGGTGCTGG AACGCCTGAT CATCGAGAAC ATCCAGCAGC AGATCGGCGA CCGCTCCGGC ATCCGCATCT CCGACGAGGA GCTGAACCAG GCGATGGGCA CCATCGCCCA GCGCAACGGC ATGAGCCTGG AGCAGTTCCA GACCGCCCTC ACCCGCGACG GCTTGTCCTA CGCCGACGCC CGCGAGCAGG TCCGTCGCGA GATGGTGATC AGCCGTGTGC GCCAGCGCCG GGTCGCCGAG CGCATCCAGG TCAGCGAGCA GGAGGTGAAG AACTTCCTTG CCTCGGACAT GGGCAAGATC CAGCTTTCCG AAGAGTACCG CCTGGCCAAT ATCCTGATCC CGGTGCCGGA AGCCGCGTCC TCGGACGTGA TCCAGGCCGC CGCAAGGCAG GCCCAGGAGC TCTACCAGCA GCTCAAGCAG GGCGCCGACT TCGGCCAACT GGCGATTTCC CGCTCGGCCG GCGACAACGC CCTGGAAGGC GGCGAGATCG GCTGGCGCAA GGCCGCCCAG CTGCCGCAGC CGTTCGACAG CATGATCGGC TCCCTGGCCG TCGGCGACGT CACCGAGCCT GTCCGCACCC CCGGCGGCTT CATCATCCTC AAGCTGGAAG AGAAGCGCGG CGGCAGCAAG ATGGTCCGTG ACGAAGTGCA TGTCCGCCAT ATCCTGCTCA AGCCCAGCGA AATCCGCAGC GAAGCGGAGA CCGAGAAGCT AGCGCAGAAG CTGTACGAGC GCATCCAGTC CGGGGAAGAC TTCGGCGAAC TGGCGAAGAG CTTCTCCGAA GATCCGGGTT CCGCCCTCAA CGGCGGCGAC CTGAACTGGA TCGATCCGGA AGCCCTGGTG CCCGAGTTCC GCCAGGTGAT GAACGATACC CCGCAGGGCG AGCTGTCCAA GCCGTTCCGC TCGCAGTTCG GCTGGCACAT CCTGCAGGTC CTCGGCCGTC GCGCCACCGA CAGCAGCGAG AAGTTCCGCG AGCAGCAGGC GGTAAGCGTA CTGCGCAACC GCAAGTACGA CGAGGAGCTG CAAGCCTGGC TGCGGCAGAT CCGCGACGAG GCCTACGTGG AAATCAAGCA GTGA

Details for PsaeA.17267.a.B2.GE38321

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	47kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Validated AA Sequence

MQSQLDQRLR EVHQTLLKRG APLPPEHVLT QQVLERLIIE NIQQQIGDRS GIRISDEELN QAMGTIAQRN GMSLEQFQTA LTRDGLSYAD AREQVRREMV ISRVRQRRVA ERIQVSEQEV KNFLASDMGK IQLSEEYRLA NILIPVPEAA SSDVIQAAAR QAQELYQQLK QGADFGQLAI SRSAGDNALE GGEIGWRKAA QLPQPFDSMI GSLAVGDVTE PVRTPGGFII LKLEEKRGGS KMVRDEVHVR HILLKPSEIR SEAETEKLAQ KLYERIQSGE DFGELAKSFS EDPGSALNGG DLNWIDPEAL VPEFRQVMND TPQGELSKPF RSQFGWHILQ VLGRRATDSS EKFREQQAVS VLRNRKYDEE LQAWLRQIR

Validated NT Sequence

tcgcggatct gccgcagcca ggcttgcagc tcctcgtcgt acttgcggtt gcgcagtacg cttaccgcct gctgctcgcg gaacttctcg ctgctgtcgg tggcgcgacg gccgaggacc tgcaggatgt gccagccgaa ctgcgagcgg aacggcttgg acagctcgcc ctgcggggta tcgttcatca cctggcggaa ctcgggcacc agggcttccg gatcgatcca gttcaggtcg ccgccgttga gggcggaacc cggatcttcg gagaagctct tcgccagttc gccgaagtct tccccggact ggatgcgctc gtacagcttc tgcgctagct tctcggtctc cgcttcgctg cggatttcgc tgggcttgag caggatatgg cggacatgca cttcgtcacg gaccatcttg ctgccgccgc gcttctcttc cagcttgagg atgatgaagc cgccgggggt gcggacaggc tcggtgacgt cgccgacggc cagggagccg atcatgctgt cgaacggctg cggcagctgg gcggccttgc gccagccgat ctcgccgcct tccagggcgt tgtcgccggc cgagcgggaa atcgccagtt ggccgaagtc ggcgccctgc ttgagctgct ggtagagctc ctgggcctgc cttgcggcgg cctggatcac gtccgaggac gcggcttccg gcaccgggat caggatattg gccaggcggt actcttcgga aagctggatc ttgcccatgt ccgaggcaag gaagttcttc acctcctgct cgctgacctg gatgcgctcg gcgacccggc gctggcgcac acggctgatc accatctcgc gacggacctg ctcgcgggcg tcggcgtagg acaagccgtc gcgggtgagg gcggtctgga actgctccag gctcatgccg ttgcgctggg cgatggtgcc catcgcctgg ttcagctcct cgtcggagat gcggatgccg gagcggtcgc cgatctgctg ctggatgttc tcgatgatca ggcgttccag cacctgctgg gtcagcacgt gctcgggcgg cagcggcgca ccgcgcttga gcagcgtctg gtgcacttca cgcaggcgct ggtcgagctg gctctgcatg atcacatcgt tgtcgacgat ggcgacgacc cggtccagtg gtacc

Expected Protein Sequence

MAHHHHHHEV VPLDRVVAIV DNDVIMQSQL DQRLREVHQT LLKRGAPLPP EHVLTQQVLE RLIIENIQQQ IGDRSGIRIS DEELNQAMGT IAQRNGMSLE QFQTALTRDG LSYADAREQV RREMVISRVR QRRVAERIQV SEQEVKNFLA SDMGKIQLSE EYRLANILIP VPEAASSDVI QAAARQAQEL YQQLKQGADF GQLAISRSAG DNALEGGEIG WRKAAQLPQP FDSMIGSLAV GDVTEPVRTP GGFIILKLEE KRGGSKMVRD EVHVRHILLK PSEIRSEAET EKLAQKLYER IQSGEDFGEL AKSFSEDPGS ALNGGDLNWI DPEALVPEFR QVMNDTPQGE LSKPFRSQFG WHILQVLGRR ATDSSEKFRE QQAVSVLRNR KYDEELQAWL RQIRDEAYVE IKQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagtgg taccactgga ccgggtcgtc gccatcgtcg acaacgatgt gatcatgcag agccagctcg accagcgcct gcgtgaagtg caccagacgc tgctcaagcg cggtgcgccg ctgccgcccg agcacgtgct gacccagcag gtgctggaac gcctgatcat cgagaacatc cagcagcaga tcggcgaccg ctccggcatc cgcatctccg acgaggagct gaaccaggcg atgggcacca tcgcccagcg caacggcatg agcctggagc agttccagac cgccctcacc cgcgacggct tgtcctacgc cgacgcccgc gagcaggtcc gtcgcgagat ggtgatcagc cgtgtgcgcc agcgccgggt cgccgagcgc atccaggtca gcgagcagga ggtgaagaac ttccttgcct cggacatggg caagatccag ctttccgaag agtaccgcct ggccaatatc ctgatcccgg tgccggaagc cgcgtcctcg gacgtgatcc aggccgccgc aaggcaggcc caggagctct accagcagct caagcagggc gccgacttcg gccaactggc gatttcccgc tcggccggcg acaacgccct ggaaggcggc gagatcggct ggcgcaaggc cgcccagctg ccgcagccgt tcgacagcat gatcggctcc ctggccgtcg gcgacgtcac cgagcctgtc cgcacccccg gcggcttcat catcctcaag ctggaagaga agcgcggcgg cagcaagatg gtccgtgacg aagtgcatgt ccgccatatc ctgctcaagc ccagcgaaat ccgcagcgaa gcggagaccg agaagctagc gcagaagctg tacgagcgca tccagtccgg ggaagacttc ggcgaactgg cgaagagctt ctccgaagat ccgggttccg ccctcaacgg cggcgacctg aactggatcg atccggaagc cctggtgccc gagttccgcc aggtgatgaa cgataccccg cagggcgagc tgtccaagcc gttccgctcg cagttcggct ggcacatcct gcaggtcctc ggccgtcgcg ccaccgacag cagcgagaag ttccgcgagc agcaggcggt aagcgtactg cgcaaccgca agtacgacga ggagctgcaa gcctggctgc ggcagatccg cgacgaggcc tacgtggaaa tcaagcagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for PsaeA.17267.a.B2.PS02183

PURIFICATION DATe:	10/14/2014
CONCENTRATION:	28mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAAGTGG TACCACTGGA CCGGGTCGTC GCCATCGTCG ACAACGATGT GATCATGCAG AGCCAGCTCG ACCAGCGCCT GCGTGAAGTG CACCAGACGC TGCTCAAGCG CGGTGCGCCG CTGCCGCCCG AGCACGTGCT GACCCAGCAG GTGCTGGAAC GCCTGATCAT CGAGAACATC CAGCAGCAGA TCGGCGACCG CTCCGGCATC CGCATCTCCG ACGAGGAGCT GAACCAGGCG ATGGGCACCA TCGCCCAGCG CAACGGCATG AGCCTGGAGC AGTTCCAGAC CGCCCTCACC CGCGACGGCT TGTCCTACGC CGACGCCCGC GAGCAGGTCC GTCGCGAGAT GGTGATCAGC CGTGTGCGCC AGCGCCGGGT CGCCGAGCGC ATCCAGGTCA GCGAGCAGGA GGTGAAGAAC TTCCTTGCCT CGGACATGGG CAAGATCCAG CTTTCCGAAG AGTACCGCCT GGCCAATATC CTGATCCCGG TGCCGGAAGC CGCGTCCTCG GACGTGATCC AGGCCGCCGC AAGGCAGGCC CAGGAGCTCT ACCAGCAGCT CAAGCAGGGC GCCGACTTCG GCCAACTGGC GATTTCCCGC TCGGCCGGCG ACAACGCCCT GGAAGGCGGC GAGATCGGCT GGCGCAAGGC CGCCCAGCTG CCGCAGCCGT TCGACAGCAT GATCGGCTCC CTGGCCGTCG GCGACGTCAC CGAGCCTGTC CGCACCCCCG GCGGCTTCAT CATCCTCAAG CTGGAAGAGA AGCGCGGCGG CAGCAAGATG GTCCGTGACG AAGTGCATGT CCGCCATATC CTGCTCAAGC CCAGCGAAAT CCGCAGCGAA GCGGAGACCG AGAAGCTAGC GCAGAAGCTG TACGAGCGCA TCCAGTCCGG GGAAGACTTC GGCGAACTGG CGAAGAGCTT CTCCGAAGAT CCGGGTTCCG CCCTCAACGG CGGCGACCTG AACTGGATCG ATCCGGAAGC CCTGGTGCCC GAGTTCCGCC AGGTGATGAA CGATACCCCG CAGGGCGAGC TGTCCAAGCC GTTCCGCTCG CAGTTCGGCT GGCACATCCT GCAGGTCCTC GGCCGTCGCG CCACCGACAG CAGCGAGAAG TTCCGCGAGC AGCAGGCGGT AAGCGTACTG CGCAACCGCA AGTACGACGA GGAGCTGCAA GCCTGGCTGC GGCAGATCCG CGACGAGGCC TACGTGGAAA TCAAGCAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT