PsaeA.17229.a

4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)

CENTER ID: PsaeA.17229.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17229.a.B1.GE38294 full length 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4769
PGAT: pseudomonas:PA4557
RefSeq: NP_253247.1
UniProt: Q9HVM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIKLANPRG FCAGVDRAIE IVNRALDVFG PPIYVRHEVV HNKFVVDNLR QRGAIFVEEL DQVPNNVIVI FSAHGVSQAV RKEAEGRGLK VFDATCPLVT KVHMEVVRYS RDGHECVLIG HEGHPEVEGT MGQYDASNGG AIYLVEDEAD VAALEVRKPE ALHYVTQTTL SMDDTSKVID ALRAKFPQIQ GPRKNDICYA TQNRQDAVKE LADQCDMVLV VGSPNSSNSN RLRELAERMG TPAYLIDGAE DMQRGWFDGV RRIGITAGAS APEVLVRGVI AQLREWGASE EQELEGREEN ITFSMPKELR VKAL
NT Sequence
atgcaaatca aactcgccaa tccccgcggc ttctgcgccg gcgtggatcg cgccatcgag atcgtcaacc gtgccctcga tgtcttcggc ccgccgatct acgtgcgtca cgaggtggtg cacaacaagt tcgtcgtgga caacctgcgc cagcgcggcg ccatcttcgt cgaggaactc gatcaggtgc cggacaacgt catcgtcatc ttcagcgccc acggcgtttc ccaggcggtc cgcaaggaag ccgaggggcg cggcctgaag gttttcgacg cgacctgccc gctggtgacc aaggtgcaca tggaagtggt gcgctacagc cgcgacggcc acgaatgcgt gctgatcggg catgaaggcc accccgaggt ggaaggcacc atgggccagt acgatgccag caacggcggt gccatctacc tggtggagga cgaggccgac gtcgccgcgc tggaggtgcg caagcccgaa gccctgcact acgtgaccca gaccaccctg tcgatggacg acacctcgaa ggtcatcgat gccctgcgcg ccaagttccc gcagatccag gggccgcgca agaacgacat ctgctatgcc acccagaacc gccaggatgc cgtgaaggaa ctggccgacc agtgcgacat ggtcctggtg gtgggcagcc ccaacagttc caactccaac cgcctgcgcg aactcgccga gcgcatgggc acgccggcct acctgatcga cggcgccgag gacatgcaac gcggctggtt cgacggtgtg cgtcgcatcg gaatcaccgc aggcgcctcc gcgccggaag tgctggtgcg cggagtgatc gcccagctac gtgagtgggg ggcgtcggaa gagcaggaac tggagggacg ggaggagaac attaccttct cgatgcccaa ggaactgcgg gtcaaggctt tgtaa
Details for PsaeA.17229.a.B1.GE38294
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ IKLANPRGFC AGVDRAIEIV NRALDVFGPP IYVRHEVVHN KFVVDNLRQR GAIFVEELDQ VPNNVIVIFS AHGVSQAVRK EAEGRGLKVF DATCPLVTKV HMEVVRYSRD GHECVLIGHE GHPEVEGTMG QYDASNGGAI YLVEDEADVA ALEVRKPEAL HYVTQTTLSM DDTSKVIDAL RAKFPQIQGP RKNDICYATQ NRQDAVKELA DQCDMVLVVG SPNSSNSNRL RELAERMGTP AYLIDGAEDM QRGWFDGVRR IGITAGASAP EVLVRGVIAQ LREWGASEEQ ELEGREENIT FSMPKELRVK AL
Validated NT Sequence
tactcactta caaagccttg acccgcagtt ccttgggcat cgagaaggta atgttctcct cccgcccctc cagttcctgc tcctccgacg ccccccactc gcgtagctgg gcgatcacgc cgcgcaccag cacttccggc gcggaagcgc ctgcggtgat tccgatgcga cgcacaccgt cgaaccagcc gcgttgcatg tcctcggcgc cgtcgatcag gtaggccggc gtgcccatcc gctcggcgag ttcgcgcagg cggttggagt tggaactgtt ggggctgccc accaccagga ccatgtcgca ctggtcggcc agttccttca cggcatcctg gcggttctgg gtggcatagc agatgtcgtt cttgcgcggt ccctggatct gcgggaactt ggcgcgcagg gcatcgatga ccttcgaggt gtcgtccatc gacagggtcg tctgggtcac gtagtgcagg gcttcgggct tgcgcacctc cagcgcggcg acgtcggcct cgtcctccac taggtagatg gcaccaccgt tgctggcatc gtactggccc atggtgcctt ccacctcggg gtgcccttca tgcccgatca gcacgcattc gtggccgtcg cggctgtagc gcaccacttc catgtgcact ttggtcacca gcgggcaggt cgcgtcgaaa accttcaggc cgcgcccctc ggcttccttg cggaccgcct gggaaacgcc gtgggcgctg aagatgacga tgacgttgtt cggcacctga tcgagttcct cgacgaagat ggcgccgcgc tggcgcaggt tgtccacgac gaacttgttg tgcaccacct cgtgacgcac gtagatcggc ggaccgaaga catcgagggc acggttgacg atctcgatgg cgcgatccac gccggcgcag aagccgcggg gattggcgag tttgatttgc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMQ IKLANPRGFC AGVDRAIEIV NRALDVFGPP IYVRHEVVHN KFVVDNLRQR GAIFVEELDQ VPNNVIVIFS AHGVSQAVRK EAEGRGLKVF DATCPLVTKV HMEVVRYSRD GHECVLIGHE GHPEVEGTMG QYDASNGGAI YLVEDEADVA ALEVRKPEAL HYVTQTTLSM DDTSKVIDAL RAKFPQIQGP RKNDICYATQ NRQDAVKELA DQCDMVLVVG SPNSSNSNRL RELAERMGTP AYLIDGAEDM QRGWFDGVRR IGITAGASAP EVLVRGVIAQ LREWGASEEQ ELEGREENIT FSMPKELRVK AL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa tcaaactcgc caatccccgc ggcttctgcg ccggcgtgga tcgcgccatc gagatcgtca accgtgccct cgatgtcttc ggcccgccga tctacgtgcg tcacgaggtg gtgcacaaca agttcgtcgt ggacaacctg cgccagcgcg gcgccatctt cgtcgaggaa ctcgatcagg tgccggacaa cgtcatcgtc atcttcagcg cccacggcgt ttcccaggcg gtccgcaagg aagccgaggg gcgcggcctg aaggttttcg acgcgacctg cccgctggtg accaaggtgc acatggaagt ggtgcgctac agccgcgacg gccacgaatg cgtgctgatc gggcatgaag gccaccccga ggtggaaggc accatgggcc agtacgatgc cagcaacggc ggtgccatct acctggtgga ggacgaggcc gacgtcgccg cgctggaggt gcgcaagccc gaagccctgc actacgtgac ccagaccacc ctgtcgatgg acgacacctc gaaggtcatc gatgccctgc gcgccaagtt cccgcagatc caggggccgc gcaagaacga catctgctat gccacccaga accgccagga tgccgtgaag gaactggccg accagtgcga catggtcctg gtggtgggca gccccaacag ttccaactcc aaccgcctgc gcgaactcgc cgagcgcatg ggcacgccgg cctacctgat cgacggcgcc gaggacatgc aacgcggctg gttcgacggt gtgcgtcgca tcggaatcac cgcaggcgcc tccgcgccgg aagtgctggt gcgcggagtg atcgcccagc tacgtgagtg gggggcgtcg gaagagcagg aactggaggg acgggaggag aacattacct tctcgatgcc caaggaactg cgggtcaagg ctttgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat