SSGCID: Target Page

PsaeA.11416.a

Uncharacterized protein (HHPRED: ATPase, cell division inhibitor)

CENTER ID:	PsaeA.11416.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.11416.a.B2.GE39840	ATPase-like domain		95	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3644
PGAT:	pseudomonas:PA3481
RefSeq:	NP_252171.1
UniProt:	Q9HYC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAITRQTVE ATLRQYQDPY LNQDPVSAGC LREVEIQGDR VRVRLELGYA AGLFRNGLAQ TLQMALEALD GVARAEVRVD CVIQPHKAQP QVEVMGNVKN IVAVASGKGG VGKSTTAANL ALALAREGAR VGILDADIYG PSQGIMFGLP EGTRPKVREQ KWFEPLEAHG VQVMSMAFLT DDSTPVVWRG PMVSGALIQL ITQTAWDNLD YLVVDMPPGT GDIQLTLAQK VPVAGAVIVT TPQDLALLDA KKGVEMFRKV NIPVLGVVEN MAVHICSNCG HAEHLFGEGG GEKLAAQFGV ELLASMPLSI AIRTQADSGR PTVIADPESQ LAMLYQEIAR HVGARIVLSE RASVGLPNIS ISDD

NT Sequence

atgtccgcta tcactcgcca aacggtggaa gccaccctcc gccagtacca ggacccctat ctgaaccagg accccgtcag cgccggctgc ctgcgcgagg tggagatcca gggcgaccgc gtgcgggtgc gcctggaact ggggtacgcc gccgggctgt tcagaaacgg cctggcgcaa accctgcaaa tggccctgga ggcgctcgac ggcgtcgctc gcgccgaggt ccgggtcgac tgcgtgatcc agccgcacaa ggcacagccg caggtcgagg tgatgggcaa cgtgaagaac atcgtcgcgg tggcttccgg caagggcggc gtcggcaagt ccaccaccgc cgccaacctg gccctggcct tggcccgcga gggtgcgcgg gtgggcatcc tcgacgcgga catctacgga cccagccagg gcatcatgtt cggcctgccg gaaggcaccc ggccgaaggt ccgtgagcag aagtggttcg agcccctgga ggcgcacggg gtgcaggtga tgtcgatggc cttcctcacc gacgacagca cgccggtggt ctggcgcggg ccgatggtct ccggtgccct gatccaactg atcacccaga ccgcctggga caacctcgac tacctggtgg tggacatgcc gccgggcacc ggcgacatcc agctgaccct ggcgcagaag gtgccggtgg ccggcgcggt gatcgtcacc accccgcagg acctggcgct gctcgacgcg aagaagggcg tggagatgtt ccgcaaggtg aacatcccgg tgctcggcgt ggtggagaac atggcggtgc atatctgctc caactgcggc catgccgagc acctgttcgg cgagggcggc ggcgagaagc tggcggcgca gttcggcgtc gagctgctgg cgtccatgcc gctgtcgatc gccatccgca cccaggccga cagcggccgg ccgacggtga tcgccgatcc ggaaagccaa ctggcgatgc tctaccagga aatcgcccgc catgtcggcg cgcgcatcgt tctcagcgag cgggccagcg tcggcctgcc gaacatcagc atcagcgacg actga

Details for PsaeA.11416.a.B2.GE39840

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	28kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG NVKNIVAVAS GKGGVGKSTT AANLALALAR EGARVGILDA DIYGPSQGIM FGLPEGTRPK VREQKWFEPL EAHGVQVMSM AFLTDDSTPV VWRGPMVSGA LIQLITQTAW DNLDYLVVDM PPGTGDIQLT LAQKVPVAGA VIVTTPQDLA LLDAKKGVEM FRKVNIPVLG VVENMAVHIC SNCGHAEHLF GEGGGEKLAA QFGVELLASM PLSIAIRTQA DSGRPTVIAD PESQLAMLYQ EIARHVGAR

Validated NT Sequence

atcttactca cttagcgcgc gccgacatgg cgggcgattt cctggtagag catcgccagt tggctttccg gatcggcgat caccgtcggc cggccgctgt cggcctgggt gcggatggcg atcgacagcg gcatggacgc cagcagctcg acgccgaact gcgccgccag cttctcgccg ccgccctcgc cgaacaggtg ctcggcatgg ccgcagttgg agcagatatg caccgccatg ttctccacca cgccgagcac cgggatgttc accttgcgga acatctccac gcccttcttc gcgtcgagca gcgccaggtc ctgcggggtg gtgacgatca ccgcgccggc caccggcacc ttctgcgcca gggtcagctg gatgtcgccg gtgcccggcg gcatgtccac caccaggtag tcgaggttgt cccaggcggt ctgggtgatc agttggatca gggcaccgga gaccatcggc ccgcgccaga ccaccggcgt gctgtcgtcg gtgaggaagg ccatcgacat cacctgcacc ccgtgcgcct ccaggggctc gaaccacttc tgctcacgga ccttcggccg ggtgccttcc ggcaggccga acatgatgcc ctggctgggt ccgtagatgt ccgcgtcgag gatgcccacc cgcgcaccct cgcgggccaa ggccagggcc aggttggcgg cggtggtgga cttgccgacg ccgcccttgc cggaagccac cgcgacgatg ttcttcacgt tgcccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca acgtgaagaa catcgtcgcg gtggcttccg gcaagggcgg cgtcggcaag tccaccaccg ccgccaacct ggccctggcc ttggcccgcg agggtgcgcg ggtgggcatc ctcgacgcgg acatctacgg acccagccag ggcatcatgt tcggcctgcc ggaaggcacc cggccgaagg tccgtgagca gaagtggttc gagcccctgg aggcgcacgg ggtgcaggtg atgtcgatgg ccttcctcac cgacgacagc acgccggtgg tctggcgcgg gccgatggtc tccggtgccc tgatccaact gatcacccag accgcctggg acaacctcga ctacctggtg gtggacatgc cgccgggcac cggcgacatc cagctgaccc tggcgcagaa ggtgccggtg gccggcgcgg tgatcgtcac caccccgcag gacctggcgc tgctcgacgc gaagaagggc gtggagatgt tccgcaaggt gaacatcccg gtgctcggcg tggtggagaa catggcggtg catatctgct ccaactgcgg ccatgccgag cacctgttcg gcgagggcgg cggcgagaag ctggcggcgc agttcggcgt cgagctgctg gcgtccatgc cgctgtcgat cgccatccgc acccaggccg acagcggccg gccgacggtg atcgccgatc cggaaagcca actggcgatg ctctaccagg aaatcgcccg ccatgtcggc gcgcgctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t