PsaeA.10825.a

Cell division protein FtsQ

CENTER ID: PsaeA.10825.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.10825.a.B2.GE38292 soluble domain 65 287
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4618
PGAT: pseudomonas:PA4409
RefSeq: NP_253099.1
RefSeq: WP_003094118.1
UniProt: G3XDA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGVLLRHQQ PGGLGRAPRK PMPRGASRLV AKEPLSVRLP KADFSFLKYL AWPLLLAVLG YGAYRGAEYI LPYADRPIAK VSVEGDLSYI SQRAVQQRIS PYLAASFFTI DLAGMRGQLE QMPWIAHAEV RRVWPDQVVI RLDEQLPIAR WGDEALLNNQ GQAFTPKELA NYEHLPRLHG PQRAQQQVMQ QYQLLSQLLR PLGFSIARLE MSDRGGWALT TAQGVEIQIG RDHVVDKIRR FVSIYDKALK DQISNIARID LRYPNGLAVA WREPVTPATV ATASAVQ
NT Sequence
atgaatggcg tactgctccg tcatcagcaa cccggtggac tcggccgcgc tccgcgcaag ccgatgccgc gcggcgccag ccgtctggtg gccaaggaac cgctgagcgt gcgcctgccg aaagccgact tcagtttcct caagtacctg gcctggccgc tgttgctggc cgttctcggc tatggcgcct atcgcggcgc cgagtacatc ctgccgtacg ccgaccggcc gatcgccaag gtcagtgtcg agggcgacct cagctacatc agccagcgcg cggtgcagca gcggatctcc ccgtacctgg cggcgagctt cttcaccatc gacctggcgg gcatgcgcgg gcaactggaa cagatgccct ggatcgccca cgccgaggtg cgccgggtgt ggccggacca ggtggtgatc cgcctggacg agcaactgcc gatcgcgcgg tggggcgacg aagcgctgct gaacaaccag ggacaggctt ttacgccaaa ggaactggcg aattatgaac atctgccccg cttgcatggt ccacagcggg cgcaacagca ggtgatgcag caataccagt tgctcagcca gttgctgcgt cccctcgggt tctccatcgc ccgcctggaa atgagcgatc gcggcggttg ggcgctgacc acggcccagg gcgtcgagat ccagatcggg cgcgaccacg tggtagacaa gattcgccgc ttcgtaagca tctacgacaa ggcactcaaa gatcagattt cgaacattgc gcggatcgac ctgcgttacc ccaacggcct cgccgttgcg tggcgcgagc cggtcacgcc cgcgacggtg gccacggcca gcgccgtgca gtga
Details for PsaeA.10825.a.B2.GE38292
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 26kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR GAEYILPYAD RPIAKVSVEG DLSYISQRAV QQRISPYLAA SFFTIDLAGM RGQLEQMPWI AHAEVRRVWP DQVVIRLDEQ LPIARWGDEA LLNNQGQAFT PKELANYEHL PRLHGPQRAQ QQVMQQYQLL SQLLRPLGFS IARLEMSDRG GWALTTAQGV EIQIGRDHVV DKIRRFVSIY DKALKDQISN IARIDLRYPN GLAVAWREPV TPATVATASA VQ
Validated NT Sequence
annntnnnnn nnntnntttt gtttaacttt aagaaaaaga tataccatgg ctcaccacca ccaccaccat atgcgcggcg ccgagtacat cctgccgtac gccgaccggc cgatcgccaa ggtcagtgtc gagggcgacc tcagctacat cagccagcgc gcggtgcagc agcggatctc cccgtacctg gcggcgagct tcttcaccat cgacctggcg ggcatgcgcg ggcaactgga acagatgccc tggatcgccc acgccgaggt gcgccgggtg tggccggacc aggtggtgat ccgcctggac gagcaactgc cgatcgcgcg gtggggcgac gaagcgctgc tgaacaacca gggacaggct tttacgccaa aggaactggc gaattatgaa catctgcccc gcttgcatgg tccacagcgg gcgcaacagc aggtgatgca gcaataccag ttgctcagcc agttgctgcg tcccctcggg ttctccatcg cccgcctgga aatgagcgat cgcggcggtt gggcgctgac cacggcccag ggcgtcgaga tccagatcgg gcgcgaccac gtggtagaca agattcgccg cttcgtaagc atctacgaca aggcactcaa agatcagatt tcgaacattg cgcggatcga cctgcgttac cccaacggcc tcgccgttgc gtggcgcgag ccggtcacgc ccgcgacggt ggccacggcc agcgccgtgc agtaagtgag taagatagga tccggctgct aacaagcccc cgaaag
Expected Protein Sequence
MAHHHHHHRG AEYILPYADR PIAKVSVEGD LSYISQRAVQ QRISPYLAAS FFTIDLAGMR GQLEQMPWIA HAEVRRVWPD QVVIRLDEQL PIARWGDEAL LNNQGQAFTP KELANYEHLP RLHGPQRAQQ QVMQQYQLLS QLLRPLGFSI ARLEMSDRGG WALTTAQGVE IQIGRDHVVD KIRRFVSIYD KALKDQISNI ARIDLRYPNG LAVAWREPVT PATVATASAV Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcggcg ccgagtacat cctgccgtac gccgaccggc cgatcgccaa ggtcagtgtc gagggcgacc tcagctacat cagccagcgc gcggtgcagc agcggatctc cccgtacctg gcggcgagct tcttcaccat cgacctggcg ggcatgcgcg ggcaactgga acagatgccc tggatcgccc acgccgaggt gcgccgggtg tggccggacc aggtggtgat ccgcctggac gagcaactgc cgatcgcgcg gtggggcgac gaagcgctgc tgaacaacca gggacaggct tttacgccaa aggaactggc gaattatgaa catctgcccc gcttgcatgg tccacagcgg gcgcaacagc aggtgatgca gcaataccag ttgctcagcc agttgctgcg tcccctcggg ttctccatcg cccgcctgga aatgagcgat cgcggcggtt gggcgctgac cacggcccag ggcgtcgaga tccagatcgg gcgcgaccac gtggtagaca agattcgccg cttcgtaagc atctacgaca aggcactcaa agatcagatt tcgaacattg cgcggatcga cctgcgttac cccaacggcc tcgccgttgc gtggcgcgag ccggtcacgc ccgcgacggt ggccacggcc agcgccgtgc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat