PsaeA.10537.a

Cell division protein FtsA

CENTER ID: PsaeA.10537.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.10537.a.B1.GE38290 full length 1 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4617
PGAT: pseudomonas:PA4408
RefSeq: NP_253098.1
UniProt: P47203

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASVQSGKMI VGLDIGTSKV VALVGEVTAD GQLEVVGIGT HPSRGLKKGV VVNIESTVQS IQRAIDEAQQ MAGCRIHSAF VGIAGNHIRS LNSHGIVAIR DREVNPADIE RVLDAAQAVA IPADQRVLHT LAQDYVIDNQ EGVREPLGMS GVRLEAKVHV VTCAVNASQN IEKCVRRCGL EVDDIILEQL ASAYSVLTED EKELGVCLVD IGGGTTDIAI FTEGAIRHTA VIPIAGDQVT NDIAMALRTP TQYAEEIKIR YACALAKLAG AGETIKVPSV GDRPPRELSR QALAEVVEPR YDELFTLVQA ELRRSGYEDL IPAGIVLTGG TSKMEGAVEL AEEIFHMPVR LGVPYSVKGL TDVVRNPIYS TGVGLLMYGL QKQSDGMSMS VSGSSYSSDE PKAPVLERLK RWVQGNF
NT Sequence
atggcaagcg tgcagagcgg caagatgatc gtcggcctcg acatcggtac ctccaaggtg gtggcgctgg tgggcgaggt gacggccgat ggccagctgg aggtcgttgg catcggtact catccgtccc gcggcctgaa gaagggcgtg gtggtgaaca tcgagtccac cgtgcagtcg atccagcgcg ccatcgacga ggcccagcag atggccggtt gccgtatcca ctcggcgttc gtcggcatcg ccggcaacca catccgcagc ctcaattccc acggcatcgt ggcgatccgc gatcgcgagg tgaacccggc ggatatcgaa cgggtactgg atgccgccca ggcggtggcg atcccagcgg accagcgggt gctgcatacc ctggcccagg attacgtgat cgataaccag gaaggcgtgc gcgagccgct gggcatgtcc ggggtgcgcc tggaagccaa ggtgcacgtg gtgacctgcg cggtgaatgc ttcgcagaac atcgagaagt gcgtgcgccg ctgcggcctc gaggtcgacg acatcatcct cgagcagttg gcctcggcct attcggtgct gaccgaggac gagaaggaac tgggcgtgtg cctggtggat atcggcggcg gcaccaccga tatcgcgatc ttcaccgaag gcgcgatccg ccataccgcg gtgatcccga tcgccggcga ccaggtcacc aatgacatcg ccatggccct gcgtacgccg acccagtacg cggaagagat caagatccgc tacgcctgcg ccctggccaa gctggccggt gccggcgaga ccatcaaggt gcccagcgtc ggcgaccggc cgccgcgcga actgtcgcgc caggccctgg ccgaagtggt cgagcctcgt tacgacgagc tgttcaccct ggtccaggcc gagctgcgcc gcagcggcta cgaggacctg atcccggcag ggatcgtcct caccggcggc acctcgaaga tggaaggcgc ggtcgagctg gccgaggaaa tcttccacat gccggtacgc ctcggcgtgc cgtatagcgt gaagggcctc accgacgtgg tgcgcaaccc gatctattcg acgggcgtcg gcctgctgat gtacggactg cagaagcagt ccgatggaat gtcgatgtct gtgtcaggca gcagctacag cagcgacgaa cccaaggcac ctgtcctgga gcggctcaaa cgctgggtcc agggcaattt ctga
Details for PsaeA.10537.a.B1.GE38290
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMA SVQSGKMIVG LDIGTSKVVA LVGEVTADGQ LEVVGIGTHP SRGLKKGVVV NIESTVQSIQ RAIDEAQQMA GCRIHSAFVG IAGNHIRSLN SHGIVAIRDR EVNPADIERV LDAAQAVAIP ADQRVLHTLA QDYVIDNQEG VREPLGMSGV RLEAKVHVVT CAVNASQNIE KCVRRCGLEV DDIILEQLAS AYSVLTEDEK ELGVCLVDIG GGTTDIAIFT EGAIRHTAVI PIAGDQVTND IAMALRTPTQ YAEEIKIRYA CALAKLAGAG ETIKVPSVGD RPPRELSRQA LAEVVEPRYD ELFTLGPGRA APQRLRGPDP XRDRPHXRXL XMEXRXSXXX IFXCXTPRXA YSXXXRXGCX XIXFXGXXXX LRTAXXXXXX XXXXX
Validated NT Sequence
annncnnnag aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tggcaagcgt gcagagcggc aagatgatcg tcggcctcga catcggtacc tccaaggtgg tggcgctggt gggcgaggtg acggccgatg gccagctgga ggtcgttggc atcggtactc atccgtcccg cggcctgaag aagggcgtgg tggtgaacat cgagtccacc gtgcagtcga tccagcgcgc catcgacgag gcccagcaga tggccggttg ccgtatccac tcggcgttcg tcggcatcgc cggcaaccac atccgcagcc tcaattccca cggcatcgtg gcgatccgcg atcgcgaggt gaacccggcg gatatcgaac gggtactgga tgccgcccag gcggtggcga tcccagcgga ccagcgggtg ctgcataccc tggcccagga ttacgtgatc gataaccagg aaggcgtgcg cgagccgctg ggcatgtccg gggtgcgcct ggaagccaag gtgcacgtgg tgacctgcgc ggtgaatgct tcgcagaaca tcgagaagtg cgtgcgccgc tgcggcctcg aggtcgacga catcatcctc gagcagttgg cctcggccta ttcggtgctg accgaggacg agaaggaact gggcgtgtgc ctggtggata tcggcggcgg caccaccgat atcgcgatct tcaccgaagg cgcgatccgc cataccgcgg tgatcccgat cgccggcgac caggtcacca atgacatcgc catggccctg cgtacgccga cccagtacgc ggaagagatc aagatccgct acgcctgcgc cctggccaag ctggccggtg ccggcgagac catcaaggtg cccagcgtcg gcgaccggcc gccgcgcgaa ctgtcgcgcc aggccctggc cgaagtggtc gagcctcgtt acgacgagct gttcaccctg ggtccaggcc gagctgcgcc gcagcggcta cgaggacctg atcccngcag ggatcgtcct caccngcggc anctcnnnat ggaanngcgg ncgagctgnn gagnaatctt cncatgccnn acncctcggc nngcgtatag cgnnnnggnn cgacnnggnt gcgnancgat ctnnttcnnn ggnnnnnnnn nnatnttacg gactgcnaan cnnnncnann naannnnnnn annnnngnnn nt
Expected Protein Sequence
MAHHHHHHMA SVQSGKMIVG LDIGTSKVVA LVGEVTADGQ LEVVGIGTHP SRGLKKGVVV NIESTVQSIQ RAIDEAQQMA GCRIHSAFVG IAGNHIRSLN SHGIVAIRDR EVNPADIERV LDAAQAVAIP ADQRVLHTLA QDYVIDNQEG VREPLGMSGV RLEAKVHVVT CAVNASQNIE KCVRRCGLEV DDIILEQLAS AYSVLTEDEK ELGVCLVDIG GGTTDIAIFT EGAIRHTAVI PIAGDQVTND IAMALRTPTQ YAEEIKIRYA CALAKLAGAG ETIKVPSVGD RPPRELSRQA LAEVVEPRYD ELFTLVQAEL RRSGYEDLIP AGIVLTGGTS KMEGAVELAE EIFHMPVRLG VPYSVKGLTD VVRNPIYSTG VGLLMYGLQK QSDGMSMSVS GSSYSSDEPK APVLERLKRW VQGNF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa gcgtgcagag cggcaagatg atcgtcggcc tcgacatcgg tacctccaag gtggtggcgc tggtgggcga ggtgacggcc gatggccagc tggaggtcgt tggcatcggt actcatccgt cccgcggcct gaagaagggc gtggtggtga acatcgagtc caccgtgcag tcgatccagc gcgccatcga cgaggcccag cagatggccg gttgccgtat ccactcggcg ttcgtcggca tcgccggcaa ccacatccgc agcctcaatt cccacggcat cgtggcgatc cgcgatcgcg aggtgaaccc ggcggatatc gaacgggtac tggatgccgc ccaggcggtg gcgatcccag cggaccagcg ggtgctgcat accctggccc aggattacgt gatcgataac caggaaggcg tgcgcgagcc gctgggcatg tccggggtgc gcctggaagc caaggtgcac gtggtgacct gcgcggtgaa tgcttcgcag aacatcgaga agtgcgtgcg ccgctgcggc ctcgaggtcg acgacatcat cctcgagcag ttggcctcgg cctattcggt gctgaccgag gacgagaagg aactgggcgt gtgcctggtg gatatcggcg gcggcaccac cgatatcgcg atcttcaccg aaggcgcgat ccgccatacc gcggtgatcc cgatcgccgg cgaccaggtc accaatgaca tcgccatggc cctgcgtacg ccgacccagt acgcggaaga gatcaagatc cgctacgcct gcgccctggc caagctggcc ggtgccggcg agaccatcaa ggtgcccagc gtcggcgacc ggccgccgcg cgaactgtcg cgccaggccc tggccgaagt ggtcgagcct cgttacgacg agctgttcac cctggtccag gccgagctgc gccgcagcgg ctacgaggac ctgatcccgg cagggatcgt cctcaccggc ggcacctcga agatggaagg cgcggtcgag ctggccgagg aaatcttcca catgccggta cgcctcggcg tgccgtatag cgtgaagggc ctcaccgacg tggtgcgcaa cccgatctat tcgacgggcg tcggcctgct gatgtacgga ctgcagaagc agtccgatgg aatgtcgatg tctgtgtcag gcagcagcta cagcagcgac gaacccaagg cacctgtcct ggagcggctc aaacgctggg tccagggcaa tttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat