PsaeA.10201.a
Methionine--tRNA ligase (EC 6.1.1.10) (Methionyl-tRNA synthetase) (MetRS)
| CENTER ID: | PsaeA.10201.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.10201.a.B2.GE43993 | truncation OB-fold deletion | 1 | 551 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.10201.a.B2.PW37938 | truncation OB-fold deletion | 1 | 551 |
Structures
7ULZ
DEPOSITED: 4/5/2022
DETERMINATION: XRay
CLONE: PsaeA.10201.a.B2.GE43993
PROTEIN: PsaeA.10201.a.B2.PW38931
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.3645 |
| PGAT: | pseudomonas:PA3482 |
| RefSeq: | NP_252172.1 |
| UniProt: | Q9HYC7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEPRKILVT SALPYANGSI HLGHMLEYIQ TDMWVRFQKM RGNQAVYVCA DDAHGSAIML RAEREGITSE QLIDAVRAEH MGDFADFLVD FDNYHSTHSE ENRELSSAIY LKLRDAGHID TRPVTQYFDP EKQMFLADRF IKGTCPKCGT ADQYGDNCEA CGATYAPTEL KDPKSAISGA TPVLKESLHY FFKLPDFEAM LKQWTRSGAL QESVANKLAE WLDSGLQQWD ISRDAPYFGF EIPDAPGKYF YVWLDAPIGY MASFKNLCAR RPELDFDAFW GKDSGAELYH FIGKDIVNFH ALFWPAMLEG AGYRKPTALN VHGYLTVNGQ KMSKSRGTFV KARTYLDHLD PEYLRYYYAS KLGRGVEDLD LNLEDFVQKV NSDLVGKVVN IASRCAGFIH KGNAGVLVGA DPAPELLAAF REAAPGIAEA YEARDFNRAM REIMALADRA NAWIAEQAPW ALAKQEGQQD KVQAVCGLGI NLFRQLVIFL KPVLPKLAAA AEAFLNVAPL TWADHQTLLA NHQLNPFQPL MTRIEPAKVE AMIEASKEDL AAASQPAGNG ELVKEPIAAE IDFDAFAAVD LRIALIEKCE FVEGADKLLR LSLDIGDAKR NVFSGIKSAY PDPSALEGRL TLYVANLAPR KMKFGVSEGM VLAAGPGGEE IYLLSPDSGA KPGQRVK
NT Sequence
atgtccgaac cacgcaagat cctcgttacc agcgccctcc cctacgccaa cggttccatt cacctgggac acatgctgga gtacatccag acggacatgt gggtgcgctt ccagaagatg cgtggcaacc aggccgtcta cgtctgcgcc gacgacgccc acggttcggc gatcatgctc cgcgccgagc gcgagggcat cacctccgag caactgatcg acgccgtgcg cgccgagcac atgggcgact tcgccgactt cctggtggac ttcgacaact accactcgac ccactcggaa gagaaccgcg agctgtccag cgcgatctac ctgaaactgc gcgacgccgg gcacatcgac acccgcccgg tgacccagta cttcgacccg gaaaagcaga tgttcctcgc cgaccgcttc atcaagggca cctgcccgaa gtgcggcacc gccgaccagt acggcgacaa ctgcgaggcc tgcggcgcga cctacgcccc gaccgaactg aaggacccga aatcggcgat ctccggcgcc accccggtgc tcaaggaatc gctgcactac ttcttcaagc tgccggactt cgaggccatg ctcaagcaat ggacccgcag cggcgccctt caggagtcgg tggccaacaa gctcgccgaa tggctggaca gcggcctcca gcagtgggac atctcgcgtg acgcgccgta cttcggcttc gagattcccg acgctcccgg caagtacttc tacgtctggc tggacgcgcc gatcggctac atggccagct tcaagaacct ctgcgcgcgg cgtcccgagc tggacttcga cgcgttctgg ggcaaggact ccggcgccga gctgtaccac ttcatcggca aggacatcgt caacttccac gcgctgttct ggccggccat gctcgaaggc gccggctacc gcaagccgac cgcgctgaac gtgcacggct acctgaccgt gaacggccag aagatgtcca agtcgcgcgg caccttcgtc aaggcgcgca cctacctcga ccacctggac ccggaatacc tgcgctacta ctacgcgtcc aagctcggcc gcggcgtcga ggacctcgac ctgaacctcg aggacttcgt gcagaaggtc aactccgacc tggtcggcaa ggtcgtcaac atcgccagcc gctgcgccgg cttcatccac aagggcaacg ccggcgtgct ggtcggcgcc gatccggcgc cggaactgct cgccgccttc cgcgaggccg caccgggcat cgccgaggcc tatgaggccc gcgacttcaa tcgcgccatg cgcgagatca tggccctcgc cgaccgcgcc aacgcctgga tcgccgaaca ggcgccctgg gcgctggcca agcaggaagg ccagcaggac aaggtccagg ccgtctgcgg cctgggcatc aacctgttcc gccagttggt gatcttcctc aagccggtgc tgccgaagct ggccgccgcc gccgaagcct tcctcaacgt cgccccgctg acctgggccg accaccagac cctgctggcc aaccaccagt tgaacccgtt ccagcccctg atgacccgta tcgagcccgc gaaagtagaa gccatgatcg aagcctccaa ggaagacctc gccgccgcat cccaacccgc cggcaacggc gagctggtca aggaacccat cgccgcggag atcgacttcg acgctttcgc cgcggtcgac ctgcgcatcg ccctgatcga gaagtgcgaa ttcgtcgaag gcgccgacaa gctgctgcgc ctgtcgctgg acatcggcga cgcgaagcgt aacgtgttct ccgggatcaa gagcgcatat cccgacccga gtgccctgga aggccgcctg acgctctatg tggccaacct ggcgccgcgc aagatgaagt tcggggtgtc cgagggcatg gtcctggccg ccggcccggg tggcgaggag atctacctgc tgagtccgga cagcggcgcc aagccgggcc agcgcgtcaa gtaa
Details for PsaeA.10201.a.B2.GE43993
| HARVESTED ON: | 12/3/2020 |
| SEQUENCED ON: | 12/16/2020 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 63kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS EPRKILVTSA LPYANGSIHL GHMLEYIQTD MWVRFQKMRG NQAVYVCADD AHGSAIMLRA EREGITSEQL IDAVRAEHMG DFADFLVDFD NYHSTHSEEN RELSSAIYLK LRDAGHIDTR PVTQYFDPEK QMFLADRFIK GTCPKCGTAD QYGDNCEACG ATYAPTELKD PKSAISGATP VLKESLHYFF KLPDFEAMLK QWTRSGALXE SVANKLAEWL DSGLQQWDIS RDAPYFGFEX PDAPXKYFYV WLDAPIGYMA SFKNLCARRP ELDFDAFWXK DSGAELYHFI GKDIVNFHAL XWPAMLEGAG YRKPXALNVH GYLTVNGQKM SKSRGTFVKA RTYLDHLDPE YLRYYYASKL GRGVEDLDLN LEDFVQKVNS DLVGKVVNIA SRCAGFIHKG NAGVLVGADP APELLAAFRE AAPGIAEAYE ARDFNRAMRE IMALADRANA WIAEQAPWAL AKQEGQQDKV QAVCGLGINL FRQLVIFLKP VLPKLAAAAE AFLNVAPLTW ADHQTLLANH QLNPFQPLMT RIEPAKVEAM IEA
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc gaaccacgca agatcctcgt taccagcgcc ctcccctacg ccaacggttc cattcacctg ggacacatgc tggagtacat ccagacggac atgtgggtgc gcttccagaa gatgcgtggc aaccaggccg tctacgtctg cgccgacgac gcccacggtt cggcgatcat gctccgcgcc gagcgcgagg gcatcacctc cgagcaactg atcgacgccg tgcgcgccga gcacatgggc gacttcgccg acttcctggt ggacttcgac aactaccact cgacccactc ggaagagaac cgcgagctgt ccagcgcgat ctacctgaaa ctgcgcgacg ccgggcacat cgacacccgc ccggtgaccc agtacttcga cccggaaaag cagatgttcc tcgccgaccg cttcatcaag ggcacctgcc cgaagtgcgg caccgccgac cagtacggcg acaactgcga ggcctgcggc gcgacctacg ccccgaccga actgaaggac ccgaaatcgg cgatctccgg cgccaccccg gtgctcaagg aatcgctgca ctacttcttc aagctgccgg acttcgaggc catgctcaag caatggaccc gcagcggcgc ccttcangag tcggtggcca acaagctcgc cgaatggctg gacagcggcc tccagcagtg ggacatctcg cgtgacgcgc cgtacttcgg cttcgagant cccgacgctc ccgncaagta cttctacgtc tggctggacg cgccgatcgg ctacatggcc agcttcaaga acctctgcgc gcggcgtccc gagctggact tcgacgcgtt ctgggncaag gactccggcg ccgagctgta ccacttcatc ggcaaggaca tcgtcaactt ccacgcgctg ntctggccgg ccatgctcga aggcgccggc taccgcaagc cgnccgcgct gaacgtgcac ggctacctga ccgtgaacgg ccagaagatg tccaagtcgc gcggcacctt cgtcaaggcg cgcacctacc tcgaccacct ggacccggaa tacctgcgct actactacgc gtccaagctc ggccgcggcg tcgaggacct cgacctgaac ctcgaggact tcgtgcagaa ggtcaactcc gacctggtcg gcaaggtcgt caacatcgcc agccgctgcg ccggcttcat ccacaagggc aacgccggcg tgctggtcgg cgccgatccg gcgccggaac tgctcgccgc cttccgcgag gccgcaccgg gcatcgccga ggcctatgag gcccgcgact tcaatcgcgc catgcgcgag atcatggccc tcgccgaccg cgccaacgcc tggatcgccg aacaggcgcc ctgggcgctg gccaagcagg aaggccagca ggacaaggtc caggccgtct gcggcctggg catcaacctg ttccgccagt tggtgatctt cctcaagccg gtgctgccga agctggccgc cgccgccgaa gccttcctca acgtcgcccc gctgacctgg gccgaccacc agaccctgct ggccaaccac cagttgaacc cgttccagcc cctgatgacc cgtatcgagc ccgcgaaagt agaagccatg atcgaagcct c
Expected Protein Sequence
MAHHHHHHMS EPRKILVTSA LPYANGSIHL GHMLEYIQTD MWVRFQKMRG NQAVYVCADD AHGSAIMLRA EREGITSEQL IDAVRAEHMG DFADFLVDFD NYHSTHSEEN RELSSAIYLK LRDAGHIDTR PVTQYFDPEK QMFLADRFIK GTCPKCGTAD QYGDNCEACG ATYAPTELKD PKSAISGATP VLKESLHYFF KLPDFEAMLK QWTRSGALQE SVANKLAEWL DSGLQQWDIS RDAPYFGFEI PDAPGKYFYV WLDAPIGYMA SFKNLCARRP ELDFDAFWGK DSGAELYHFI GKDIVNFHAL FWPAMLEGAG YRKPTALNVH GYLTVNGQKM SKSRGTFVKA RTYLDHLDPE YLRYYYASKL GRGVEDLDLN LEDFVQKVNS DLVGKVVNIA SRCAGFIHKG NAGVLVGADP APELLAAFRE AAPGIAEAYE ARDFNRAMRE IMALADRANA WIAEQAPWAL AKQEGQQDKV QAVCGLGINL FRQLVIFLKP VLPKLAAAAE AFLNVAPLTW ADHQTLLANH QLNPFQPLMT RIEPAKVEAM IEASKEDLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aaccacgcaa gatcctcgtt accagcgccc tcccctacgc caacggttcc attcacctgg gacacatgct ggagtacatc cagacggaca tgtgggtgcg cttccagaag atgcgtggca accaggccgt ctacgtctgc gccgacgacg cccacggttc ggcgatcatg ctccgcgccg agcgcgaggg catcacctcc gagcaactga tcgacgccgt gcgcgccgag cacatgggcg acttcgccga cttcctggtg gacttcgaca actaccactc gacccactcg gaagagaacc gcgagctgtc cagcgcgatc tacctgaaac tgcgcgacgc cgggcacatc gacacccgcc cggtgaccca gtacttcgac ccggaaaagc agatgttcct cgccgaccgc ttcatcaagg gcacctgccc gaagtgcggc accgccgacc agtacggcga caactgcgag gcctgcggcg cgacctacgc cccgaccgaa ctgaaggacc cgaaatcggc gatctccggc gccaccccgg tgctcaagga atcgctgcac tacttcttca agctgccgga cttcgaggcc atgctcaagc aatggacccg cagcggcgcc cttcaggagt cggtggccaa caagctcgcc gaatggctgg acagcggcct ccagcagtgg gacatctcgc gtgacgcgcc gtacttcggc ttcgagattc ccgacgctcc cggcaagtac ttctacgtct ggctggacgc gccgatcggc tacatggcca gcttcaagaa cctctgcgcg cggcgtcccg agctggactt cgacgcgttc tggggcaagg actccggcgc cgagctgtac cacttcatcg gcaaggacat cgtcaacttc cacgcgctgt tctggccggc catgctcgaa ggcgccggct accgcaagcc gaccgcgctg aacgtgcacg gctacctgac cgtgaacggc cagaagatgt ccaagtcgcg cggcaccttc gtcaaggcgc gcacctacct cgaccacctg gacccggaat acctgcgcta ctactacgcg tccaagctcg gccgcggcgt cgaggacctc gacctgaacc tcgaggactt cgtgcagaag gtcaactccg acctggtcgg caaggtcgtc aacatcgcca gccgctgcgc cggcttcatc cacaagggca acgccggcgt gctggtcggc gccgatccgg cgccggaact gctcgccgcc ttccgcgagg ccgcaccggg catcgccgag gcctatgagg cccgcgactt caatcgcgcc atgcgcgaga tcatggccct cgccgaccgc gccaacgcct ggatcgccga acaggcgccc tgggcgctgg ccaagcagga aggccagcag gacaaggtcc aggccgtctg cggcctgggc atcaacctgt tccgccagtt ggtgatcttc ctcaagccgg tgctgccgaa gctggccgcc gccgccgaag ccttcctcaa cgtcgccccg ctgacctggg ccgaccacca gaccctgctg gccaaccacc agttgaaccc gttccagccc ctgatgaccc gtatcgagcc cgcgaaagta gaagccatga tcgaagcctc caaggaagac ctcgcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.10201.a.B2.PW37938
| PURIFICATION DATe: | 8/8/2016 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS EPRKILVTSA LPYANGSIHL GHMLEYIQTD MWVRFQKMRG NQAVYVCADD AHGSAIMLRA EREGITSEQL IDAVRAEHMG DFADFLVDFD NYHSTHSEEN RELSSAIYLK LRDAGHIDTR PVTQYFDPEK QMFLADRFIK GTCPKCGTAD QYGDNCEACG ATYAPTELKD PKSAISGATP VLKESLHYFF KLPDFEAMLK QWTRSGALXE SVANKLAEWL DSGLQQWDIS RDAPYFGFEX PDAPXKYFYV WLDAPIGYMA SFKNLCARRP ELDFDAFWXK DSGAELYHFI GKDIVNFHAL XWPAMLEGAG YRKPXALNVH GYLTVNGQKM SKSRGTFVKA RTYLDHLDPE YLRYYYASKL GRGVEDLDLN LEDFVQKVNS DLVGKVVNIA SRCAGFIHKG NAGVLVGADP APELLAAFRE AAPGIAEAYE ARDFNRAMRE IMALADRANA WIAEQAPWAL AKQEGQQDKV QAVCGLGINL FRQLVIFLKP VLPKLAAAAE AFLNVAPLTW ADHQTLLANH QLNPFQPLMT RIEPAKVEAM IEA
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc gaaccacgca agatcctcgt taccagcgcc ctcccctacg ccaacggttc cattcacctg ggacacatgc tggagtacat ccagacggac atgtgggtgc gcttccagaa gatgcgtggc aaccaggccg tctacgtctg cgccgacgac gcccacggtt cggcgatcat gctccgcgcc gagcgcgagg gcatcacctc cgagcaactg atcgacgccg tgcgcgccga gcacatgggc gacttcgccg acttcctggt ggacttcgac aactaccact cgacccactc ggaagagaac cgcgagctgt ccagcgcgat ctacctgaaa ctgcgcgacg ccgggcacat cgacacccgc ccggtgaccc agtacttcga cccggaaaag cagatgttcc tcgccgaccg cttcatcaag ggcacctgcc cgaagtgcgg caccgccgac cagtacggcg acaactgcga ggcctgcggc gcgacctacg ccccgaccga actgaaggac ccgaaatcgg cgatctccgg cgccaccccg gtgctcaagg aatcgctgca ctacttcttc aagctgccgg acttcgaggc catgctcaag caatggaccc gcagcggcgc ccttcangag tcggtggcca acaagctcgc cgaatggctg gacagcggcc tccagcagtg ggacatctcg cgtgacgcgc cgtacttcgg cttcgagant cccgacgctc ccgncaagta cttctacgtc tggctggacg cgccgatcgg ctacatggcc agcttcaaga acctctgcgc gcggcgtccc gagctggact tcgacgcgtt ctgggncaag gactccggcg ccgagctgta ccacttcatc ggcaaggaca tcgtcaactt ccacgcgctg ntctggccgg ccatgctcga aggcgccggc taccgcaagc cgnccgcgct gaacgtgcac ggctacctga ccgtgaacgg ccagaagatg tccaagtcgc gcggcacctt cgtcaaggcg cgcacctacc tcgaccacct ggacccggaa tacctgcgct actactacgc gtccaagctc ggccgcggcg tcgaggacct cgacctgaac ctcgaggact tcgtgcagaa ggtcaactcc gacctggtcg gcaaggtcgt caacatcgcc agccgctgcg ccggcttcat ccacaagggc aacgccggcg tgctggtcgg cgccgatccg gcgccggaac tgctcgccgc cttccgcgag gccgcaccgg gcatcgccga ggcctatgag gcccgcgact tcaatcgcgc catgcgcgag atcatggccc tcgccgaccg cgccaacgcc tggatcgccg aacaggcgcc ctgggcgctg gccaagcagg aaggccagca ggacaaggtc caggccgtct gcggcctggg catcaacctg ttccgccagt tggtgatctt cctcaagccg gtgctgccga agctggccgc cgccgccgaa gccttcctca acgtcgcccc gctgacctgg gccgaccacc agaccctgct ggccaaccac cagttgaacc cgttccagcc cctgatgacc cgtatcgagc ccgcgaaagt agaagccatg atcgaagcct c
Expressed Protein Sequence
MAHHHHHHMS EPRKILVTSA LPYANGSIHL GHMLEYIQTD MWVRFQKMRG NQAVYVCADD AHGSAIMLRA EREGITSEQL IDAVRAEHMG DFADFLVDFD NYHSTHSEEN RELSSAIYLK LRDAGHIDTR PVTQYFDPEK QMFLADRFIK GTCPKCGTAD QYGDNCEACG ATYAPTELKD PKSAISGATP VLKESLHYFF KLPDFEAMLK QWTRSGALQE SVANKLAEWL DSGLQQWDIS RDAPYFGFEI PDAPGKYFYV WLDAPIGYMA SFKNLCARRP ELDFDAFWGK DSGAELYHFI GKDIVNFHAL FWPAMLEGAG YRKPTALNVH GYLTVNGQKM SKSRGTFVKA RTYLDHLDPE YLRYYYASKL GRGVEDLDLN LEDFVQKVNS DLVGKVVNIA SRCAGFIHKG NAGVLVGADP APELLAAFRE AAPGIAEAYE ARDFNRAMRE IMALADRANA WIAEQAPWAL AKQEGQQDKV QAVCGLGINL FRQLVIFLKP VLPKLAAAAE AFLNVAPLTW ADHQTLLANH QLNPFQPLMT RIEPAKVEAM IEASKEDLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG AACCACGCAA GATCCTCGTT ACCAGCGCCC TCCCCTACGC CAACGGTTCC ATTCACCTGG GACACATGCT GGAGTACATC CAGACGGACA TGTGGGTGCG CTTCCAGAAG ATGCGTGGCA ACCAGGCCGT CTACGTCTGC GCCGACGACG CCCACGGTTC GGCGATCATG CTCCGCGCCG AGCGCGAGGG CATCACCTCC GAGCAACTGA TCGACGCCGT GCGCGCCGAG CACATGGGCG ACTTCGCCGA CTTCCTGGTG GACTTCGACA ACTACCACTC GACCCACTCG GAAGAGAACC GCGAGCTGTC CAGCGCGATC TACCTGAAAC TGCGCGACGC CGGGCACATC GACACCCGCC CGGTGACCCA GTACTTCGAC CCGGAAAAGC AGATGTTCCT CGCCGACCGC TTCATCAAGG GCACCTGCCC GAAGTGCGGC ACCGCCGACC AGTACGGCGA CAACTGCGAG GCCTGCGGCG CGACCTACGC CCCGACCGAA CTGAAGGACC CGAAATCGGC GATCTCCGGC GCCACCCCGG TGCTCAAGGA ATCGCTGCAC TACTTCTTCA AGCTGCCGGA CTTCGAGGCC ATGCTCAAGC AATGGACCCG CAGCGGCGCC CTTCAGGAGT CGGTGGCCAA CAAGCTCGCC GAATGGCTGG ACAGCGGCCT CCAGCAGTGG GACATCTCGC GTGACGCGCC GTACTTCGGC TTCGAGATTC CCGACGCTCC CGGCAAGTAC TTCTACGTCT GGCTGGACGC GCCGATCGGC TACATGGCCA GCTTCAAGAA CCTCTGCGCG CGGCGTCCCG AGCTGGACTT CGACGCGTTC TGGGGCAAGG ACTCCGGCGC CGAGCTGTAC CACTTCATCG GCAAGGACAT CGTCAACTTC CACGCGCTGT TCTGGCCGGC CATGCTCGAA GGCGCCGGCT ACCGCAAGCC GACCGCGCTG AACGTGCACG GCTACCTGAC CGTGAACGGC CAGAAGATGT CCAAGTCGCG CGGCACCTTC GTCAAGGCGC GCACCTACCT CGACCACCTG GACCCGGAAT ACCTGCGCTA CTACTACGCG TCCAAGCTCG GCCGCGGCGT CGAGGACCTC GACCTGAACC TCGAGGACTT CGTGCAGAAG GTCAACTCCG ACCTGGTCGG CAAGGTCGTC AACATCGCCA GCCGCTGCGC CGGCTTCATC CACAAGGGCA ACGCCGGCGT GCTGGTCGGC GCCGATCCGG CGCCGGAACT GCTCGCCGCC TTCCGCGAGG CCGCACCGGG CATCGCCGAG GCCTATGAGG CCCGCGACTT CAATCGCGCC ATGCGCGAGA TCATGGCCCT CGCCGACCGC GCCAACGCCT GGATCGCCGA ACAGGCGCCC TGGGCGCTGG CCAAGCAGGA AGGCCAGCAG GACAAGGTCC AGGCCGTCTG CGGCCTGGGC ATCAACCTGT TCCGCCAGTT GGTGATCTTC CTCAAGCCGG TGCTGCCGAA GCTGGCCGCC GCCGCCGAAG CCTTCCTCAA CGTCGCCCCG CTGACCTGGG CCGACCACCA GACCCTGCTG GCCAACCACC AGTTGAACCC GTTCCAGCCC CTGATGACCC GTATCGAGCC CGCGAAAGTA GAAGCCATGA TCGAAGCCTC CAAGGAAGAC CTCGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T