SSGCID: Target Page

PsaeA.01643.a

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13) (Meso-A2pm-adding enzyme) (Meso-diaminopimelate-adding enzyme) (UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase) (UDP-MurNAc-tripeptide synthetase) (UDP-N-acetylmuramyl-tripeptide synthetase) (MurE)

CENTER ID:	PsaeA.01643.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01643.a.B1.GE38288	full length		1	487

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01643.a.B1.PS02199	full length		1	487

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4626
PGAT:	pseudomonas:PA4417
RefSeq:	NP_253107.1
UniProt:	Q59650

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPMSLNQLFP QAERDLLIRE LTLDSRGVRP GDLFLAVPGG RQDGRAHIAD ALAKGAAAVA YEAEGAGELP PSDAPLIAVK GLAAQLSAVA GRFYGEPSRG LDLIGVTGTN GKTSVSQLVA QALDLLGERC GIVGTLGTGF YGALESGRHT TPDPLAVQAT LATLKQAGAR AVAMEVSSHG LDQGRVAALG FDIAVFTNLS RDHLDYHGSM EAYAAAKAKL FAWPGLRCRV INLDDDFGRR LAGEEQDSEL ITYSLTDSSA FLYCREARFG DAGIEAALVT PHGEGLLRSP LLGRFNLSNL LAAVGALLGL GYPLGDILRT LPQLQGPVGR MQRLGGGDKP LVVVDYAHTP DALEKVLEAL RPHAAARLLC LFGCGGDRDA GKRPLMAAIA ERLADEVLVT DDNPRTEASA AIIADIRKGF AAADKVTFLP SRGEAIAHLI ASAAVDDVVL LAGKGHEDYQ EIDGVRHPFS DIEQAERALA AWEVPHA

NT Sequence

atgcctatga gcctgaacca actgtttccc caggccgagc gcgatctgct gatccgcgag ctgaccctgg atagccgcgg cgttcgtccg ggcgacctgt tcctggcggt gccgggcggg cgccaggatg gtcgtgcgca catcgccgat gccctggcca agggcgcggc tgccgtggct tacgaggcgg aaggcgccgg agagttgccg cccagcgatg cgccgctgat cgcggtgaag gggctggccg cgcaactgtc ggcggtcgcc gggcgtttct acggcgagcc gagccgcggg ctggacctga tcggcgtcac cggcaccaac ggcaagacca gcgtcagcca actggtggcc caggccctgg atctgctcgg cgagcgctgc ggcatcgtcg gcaccctcgg caccggtttc tacggcgccc tggagagcgg ccggcacacc acgccggacc cgctcgcggt gcaggccacg ctggccacgc tgaagcaggc cggcgcccgc gcggtagcga tggaagtgtc ttcccacggc ctcgaccagg gccgcgtggc ggcgctcggt ttcgatatcg cggtgttcac caatctgtcc cgcgaccacc tcgactatca cggttcgatg gaagcctatg ccgccgccaa ggccaagctg ttcgcctggc cgggcctgcg ctgccgggtg atcaacctgg acgacgattt cggccgtcga ctggccggcg aggagcagga ctcggagctg atcacctaca gcctcaccga cagctcggcg ttcctctatt gccgcgaagc gcgcttcggc gacgccggca tcgaggcggc gctggtcact ccgcacggcg agggcctgct gcgcagcccg ttgctcggcc gcttcaacct gagcaacctg ctggcggcgg tcggtgcgtt gcttggcctg ggttatcccc tgggcgatat cctccgcact ttgccgcaac tgcaggggcc ggtcggccgc atgcagcgcc tgggaggcgg cgacaagccg ctggtggtgg tggactacgc gcatactccc gacgccctgg aaaaagtcct ggaggccctg cgtccgcacg cggccgcgcg cctgctgtgc ctgttcggct gcggtggcga tcgcgatgcc ggcaagcgtc cgctgatggc tgcgatcgcc gaacgcctgg cggatgaggt gctggtcacc gacgacaacc cgcgcaccga ggccagtgcg gcgatcatcg ccgatatccg caaaggcttc gctgccgctg acaaggttac cttcctgccg tcgcgcggtg aggcgatcgc ccatctgatc gcttccgctg cggtggatga cgtggtgctc ctggccggca agggtcacga ggattatcag gagatcgacg gcgtacgcca tccgttctcc gacatcgagc aggccgagcg cgccctggcc gcctgggagg tgccgcatgc ttga

Details for PsaeA.01643.a.B1.GE38288

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Validated AA Sequence

MSLNQLFPQA ERDLLIRELT LDSRGVRPGD LFLAVPGGRQ DGRAHIADAL AKGAAAVAYE AEGAGELPPS DAPLIAVKGL AAQLSAVAGR FYGEPSRGLD LIGVTGTNGK TSVSQLVAQA LDLLGERCGI VGTLGTGFYG ALESGRHTTP DPLAVQATLA TLKQAGARAV AMEVSSHGLD QGRVAALGFD IAVFTNLSRD HLDYHGSMEA YAAAKAKLFA WPGLRCRVIN LDDDFGRRLA GEEQXSELIT YSLTDSSAFL YCREARFGDA GIEAALVTPH GEGLLRSPLL GRFNLSNLLA AVGALLGLGY PLGDILRTLP QLQGPVGRMQ RLGGGDKPLV VVDYAHTPDA LEKVLEALRP HAAARLLCLF GCGGDRDAGK RPXMAAIAER LADEVLVTDD NPRTEASAAI IADIRKGFAA ADKVTFLPSR GEAIAHXIAS AAVDDVVLLA XKGHEDYQEI DGVRHPFSDI EQAERA

Validated NT Sequence

agggcgcgct cggcctgctc gatgtcggag aacggatggc gtacgccgtc gatctcctga taatcctcgt gacccttgnc ggccaggagc accacgtcat ccaccgcagc ggaagcgatc anatgggcga tcgcctcacc gcgcgacggc aggaaggtaa ccttgtcagc ggcagcgaag cctttgcgga tatcggcgat gatcgccgca ctggcctcgg tgcgcgggtt gtcgtcggtg accagcacct catccgccag gcgttcggcg atcgcagcca tcancggacg cttgccggca tcgcgatcgc caccgcagcc gaacaggcac agcaggcgcg cggccgcgtg nggacgcagg gcctccagga ctttttccag ggcgtcggga gtatgcgcgt agtccaccac caccagcggc ttgtcgccgc ctcccaggcg ctgcatgcgg ccgaccggcc cctgcagttg cggcaaagtg cggaggatat cgcccagggg ataacccagg ccaagcaacg caccgaccgc cgccagcagg ttgctcaggt tgaagcggcc gagcaacggg ctgcgcagca ggccctcgcc gtgcggagtg accagcgccg cctcgatgcc ggcgtcgccg aagcgcgctt cgcggcaata gaggaacgcc gagctgtcgg tgaggctgta ggtgatcagc tccgantcct gctcctcgcc ggccagtcga cggccgaaat cgtcgtccag gttgatcacc cggcagcgca ggcccggcca ggcgaacagc ttggccttgg cggcggcata ggcttccatc gaaccgtgat agtcgaggtg gtcgcgggac agattggtga acaccgcgat atcgaaaccg agcgccgcca cgcggccctg gtcgaggccg tgggaagaca cttccatcgc taccgcgcgg gcgccggcct gcttcagcgt ggccagcgtg gcctgcaccg cgagcgggtc cggcgtggtg tgccggccgc tctccagggc gccgtagaaa ccggtgccga gggtgccgac gatgccgcag cgctcgccga gcagatccag ggcctgggcc accagttggc tgacgctggt cttgccgttg gtgccggtga cgccgatcag gtccagcccg cggctcggct cgccgtagaa acgcccggcg accgccgaca gttgcgcggc cagccccttc accgcgatca gcggcgcatc gctgggcggc aactctccgg cgccttccgc ctcgtaagcc acggcagccg cgcccttggc cagggcatcg gcgatgtgcg cacgaccatc ctggcgcccg cccggcaccg ccaggaacag gtcgcccgga cgaacgccgc ggctatccag ggtcagctcg cggatcagca gatcgcgctc ggcctgggga aacagttggt tcaggctcat agg

Expected Protein Sequence

MAHHHHHHMP MSLNQLFPQA ERDLLIRELT LDSRGVRPGD LFLAVPGGRQ DGRAHIADAL AKGAAAVAYE AEGAGELPPS DAPLIAVKGL AAQLSAVAGR FYGEPSRGLD LIGVTGTNGK TSVSQLVAQA LDLLGERCGI VGTLGTGFYG ALESGRHTTP DPLAVQATLA TLKQAGARAV AMEVSSHGLD QGRVAALGFD IAVFTNLSRD HLDYHGSMEA YAAAKAKLFA WPGLRCRVIN LDDDFGRRLA GEEQDSELIT YSLTDSSAFL YCREARFGDA GIEAALVTPH GEGLLRSPLL GRFNLSNLLA AVGALLGLGY PLGDILRTLP QLQGPVGRMQ RLGGGDKPLV VVDYAHTPDA LEKVLEALRP HAAARLLCLF GCGGDRDAGK RPLMAAIAER LADEVLVTDD NPRTEASAAI IADIRKGFAA ADKVTFLPSR GEAIAHLIAS AAVDDVVLLA GKGHEDYQEI DGVRHPFSDI EQAERALAAW EVPHA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccta tgagcctgaa ccaactgttt ccccaggccg agcgcgatct gctgatccgc gagctgaccc tggatagccg cggcgttcgt ccgggcgacc tgttcctggc ggtgccgggc gggcgccagg atggtcgtgc gcacatcgcc gatgccctgg ccaagggcgc ggctgccgtg gcttacgagg cggaaggcgc cggagagttg ccgcccagcg atgcgccgct gatcgcggtg aaggggctgg ccgcgcaact gtcggcggtc gccgggcgtt tctacggcga gccgagccgc gggctggacc tgatcggcgt caccggcacc aacggcaaga ccagcgtcag ccaactggtg gcccaggccc tggatctgct cggcgagcgc tgcggcatcg tcggcaccct cggcaccggt ttctacggcg ccctggagag cggccggcac accacgccgg acccgctcgc ggtgcaggcc acgctggcca cgctgaagca ggccggcgcc cgcgcggtag cgatggaagt gtcttcccac ggcctcgacc agggccgcgt ggcggcgctc ggtttcgata tcgcggtgtt caccaatctg tcccgcgacc acctcgacta tcacggttcg atggaagcct atgccgccgc caaggccaag ctgttcgcct ggccgggcct gcgctgccgg gtgatcaacc tggacgacga tttcggccgt cgactggccg gcgaggagca ggactcggag ctgatcacct acagcctcac cgacagctcg gcgttcctct attgccgcga agcgcgcttc ggcgacgccg gcatcgaggc ggcgctggtc actccgcacg gcgagggcct gctgcgcagc ccgttgctcg gccgcttcaa cctgagcaac ctgctggcgg cggtcggtgc gttgcttggc ctgggttatc ccctgggcga tatcctccgc actttgccgc aactgcaggg gccggtcggc cgcatgcagc gcctgggagg cggcgacaag ccgctggtgg tggtggacta cgcgcatact cccgacgccc tggaaaaagt cctggaggcc ctgcgtccgc acgcggccgc gcgcctgctg tgcctgttcg gctgcggtgg cgatcgcgat gccggcaagc gtccgctgat ggctgcgatc gccgaacgcc tggcggatga ggtgctggtc accgacgaca acccgcgcac cgaggccagt gcggcgatca tcgccgatat ccgcaaaggc ttcgctgccg ctgacaaggt taccttcctg ccgtcgcgcg gtgaggcgat cgcccatctg atcgcttccg ctgcggtgga tgacgtggtg ctcctggccg gcaagggtca cgaggattat caggagatcg acggcgtacg ccatccgttc tccgacatcg agcaggccga gcgcgccctg gccgcctggg aggtgccgca tgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for PsaeA.01643.a.B1.PS02199

PURIFICATION DATe:	11/4/2014
CONCENTRATION:	27.6mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTA TGAGCCTGAA CCAACTGTTT CCCCAGGCCG AGCGCGATCT GCTGATCCGC GAGCTGACCC TGGATAGCCG CGGCGTTCGT CCGGGCGACC TGTTCCTGGC GGTGCCGGGC GGGCGCCAGG ATGGTCGTGC GCACATCGCC GATGCCCTGG CCAAGGGCGC GGCTGCCGTG GCTTACGAGG CGGAAGGCGC CGGAGAGTTG CCGCCCAGCG ATGCGCCGCT GATCGCGGTG AAGGGGCTGG CCGCGCAACT GTCGGCGGTC GCCGGGCGTT TCTACGGCGA GCCGAGCCGC GGGCTGGACC TGATCGGCGT CACCGGCACC AACGGCAAGA CCAGCGTCAG CCAACTGGTG GCCCAGGCCC TGGATCTGCT CGGCGAGCGC TGCGGCATCG TCGGCACCCT CGGCACCGGT TTCTACGGCG CCCTGGAGAG CGGCCGGCAC ACCACGCCGG ACCCGCTCGC GGTGCAGGCC ACGCTGGCCA CGCTGAAGCA GGCCGGCGCC CGCGCGGTAG CGATGGAAGT GTCTTCCCAC GGCCTCGACC AGGGCCGCGT GGCGGCGCTC GGTTTCGATA TCGCGGTGTT CACCAATCTG TCCCGCGACC ACCTCGACTA TCACGGTTCG ATGGAAGCCT ATGCCGCCGC CAAGGCCAAG CTGTTCGCCT GGCCGGGCCT GCGCTGCCGG GTGATCAACC TGGACGACGA TTTCGGCCGT CGACTGGCCG GCGAGGAGCA GGACTCGGAG CTGATCACCT ACAGCCTCAC CGACAGCTCG GCGTTCCTCT ATTGCCGCGA AGCGCGCTTC GGCGACGCCG GCATCGAGGC GGCGCTGGTC ACTCCGCACG GCGAGGGCCT GCTGCGCAGC CCGTTGCTCG GCCGCTTCAA CCTGAGCAAC CTGCTGGCGG CGGTCGGTGC GTTGCTTGGC CTGGGTTATC CCCTGGGCGA TATCCTCCGC ACTTTGCCGC AACTGCAGGG GCCGGTCGGC CGCATGCAGC GCCTGGGAGG CGGCGACAAG CCGCTGGTGG TGGTGGACTA CGCGCATACT CCCGACGCCC TGGAAAAAGT CCTGGAGGCC CTGCGTCCGC ACGCGGCCGC GCGCCTGCTG TGCCTGTTCG GCTGCGGTGG CGATCGCGAT GCCGGCAAGC GTCCGCTGAT GGCTGCGATC GCCGAACGCC TGGCGGATGA GGTGCTGGTC ACCGACGACA ACCCGCGCAC CGAGGCCAGT GCGGCGATCA TCGCCGATAT CCGCAAAGGC TTCGCTGCCG CTGACAAGGT TACCTTCCTG CCGTCGCGCG GTGAGGCGAT CGCCCATCTG ATCGCTTCCG CTGCGGTGGA TGACGTGGTG CTCCTGGCCG GCAAGGGTCA CGAGGATTAT CAGGAGATCG ACGGCGTACG CCATCCGTTC TCCGACATCG AGCAGGCCGA GCGCGCCCTG GCCGCCTGGG AGGTGCCGCA TGCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT