PsaeA.01608.a

Ribonucleoside-diphosphate reductase subunit beta (EC 1.17.4.1)

CENTER ID: PsaeA.01608.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.01608.a.B3.GE38285 soluble domain #2 273 415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.1200
PGAT: pseudomonas:PA1155
RefSeq: NP_249846.1
UniProt: Q9I4I2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSWDEFDKE DPTEAKAAPA AAQAVAQGHD KLDDEAAGSV EEARAVSADD SDAVARAKKA LNDLDIQEGL DDLEGSAARV QVGDKQMINA RADLNQLVPF KYDWAWQKYL DGCANHWMPQ EVNMNADIAL WKSKDGLSED ERRIVMRNLG FFSTADSLVA NNLVLAVYRL ITNPECRQYI LRQAFEEAIH THAYQYCIES LGMDEGEIFN MYHEIPSVAK KASWGLKYTR SISDPMFQTG TPETDRQFLR NLIAYYCVLE GIFFYCGFTQ ILSMGRRNKM TGTAEQFQYI LRDESMHLNF GIDVINQIKI ENPHLWDAQM KDEATQMILQ GTQLEIEYAR DTMPRGVLGM NAAMMEDYLK FIANRRLTQI GLKEEYPGTT NPFPWMSEIM DLKKEKNFFE TRVIEYQTGG ALSWD
NT Sequence
atgctgagct gggacgaatt cgacaaagaa gacccgaccg aagccaaggc cgcgcccgcc gccgcccagg ccgttgccca gggccatgac aagctggacg acgaagccgc cggctcggtg gaagaagcgc gcgccgtttc cgccgacgac tccgacgctg tcgcccgcgc caagaaggcc ctgaacgacc tcgacatcca ggaaggcttg gacgacctcg aaggctccgc cgcgcgcgtg caggtcggcg acaagcagat gatcaacgcc cgtgccgacc tcaaccagct cgttcccttc aagtacgact gggcctggca gaagtatctg gatggttgcg ccaaccactg gatgccgcag gaagtcaaca tgaacgccga catcgccctg tggaagagca aggacggcct cagcgaagac gagcggcgca tcgtcatgcg caacctcggc ttcttctcca ccgccgactc gctggtcgcc aacaacctgg tgctggccgt gtaccgcctg atcaccaacc ccgagtgccg ccagtacatc ctgcgccagg ccttcgaaga ggcgatccac acccacgcct accagtactg catcgagtcg ctgggcatgg acgagggcga gatcttcaac atgtaccacg agatccccag cgtggcgaag aaggcctcct ggggcctgaa gtacacccgc tcgatctccg acccgatgtt ccagaccggc accccggaaa ccgaccgcca gttcctgcgc aacctgatcg cctactactg cgtgctggaa ggcatcttct tctactgcgg cttcacccag atcctctcca tgggccgccg caacaagatg accggcaccg ccgagcagtt ccagtacatc ctccgcgacg agtcgatgca cctgaacttc ggtatcgacg tgatcaacca gatcaagatc gagaacccgc acctgtggga cgcccagatg aaggacgagg cgacccagat gatcctccag ggcacccagc tggagatcga atacgcgcgc gacaccatgc cgcgcggggt gctgggcatg aacgcggcga tgatggagga ctacctgaag ttcatcgcca accggcgcct gacccagatc ggcctgaagg aagagtatcc ggggaccacc aacccgttcc cgtggatgtc ggagatcatg gacctgaaga aggagaagaa cttcttcgag acgcgggtta tcgagtacca gacgggcgga gcactgagct gggattga
Details for PsaeA.01608.a.B3.GE38285
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS MGRRNKMTGT AEQFQYILRD ESMHLNFGID VINQIKIENP HLWDAQMKDE ATQMILQGTQ LEIEYARDTM PRGVLGMNAA MMEDYLKFIA NRRLTQIGLK EEYPGTTNPF PWMSEIMDLK KEKNFFETRV IEYQTGGALS WD
Validated NT Sequence
tcnntnnnan nattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgt ccatgggccg ccgcaacaag atgaccggca ccgccgagca gttccagtac atcctccgcg acgagtcgat gcacctgaac ttcggtatcg acgtgatcaa ccagatcaag atcgagaacc cgcacctgtg ggacgcccag atgaaggacg aggcgaccca gatgatcctc cagggcaccc agctggagat cgaatacgcg cgcgacacca tgccgcgcgg ggtgctgggc atgaacgcgg cgatgatgga ggactacctg aagttcatcg ccaaccggcg cctgacccag atcggcctga aggaagagta tccggggacc accaacccgt tcccgtggat gtcggagatc atggacctga agaaggagaa gaacttcttc gagacgcggg ttatcgagta ccagacgggc ggagcactga gctgggatta agtgagtaag ataggatccg gctgctaaca gncccccgaa agaaggttnt naanaaaatt cgntnacnna gacgactccg tccattagag cgacacatcc taatgaatct acgacctgtg ctccaagttc ctacacatct gagggaccgc gatcgaacga tgtctgctan gctgccnnnc ancgtcatga tgccgatgag gagcaggtgc accacngcna catggtcctg gtggagggca gnnnnatagt tccaactcca nntcctgana aactcgtcta ccggatggnc acaccgctac ctaatcattg ctctgagnac tgtcnatact ctagttatga cgatnagtnn tcnnacng
Expected Protein Sequence
MAHHHHHHSM GRRNKMTGTA EQFQYILRDE SMHLNFGIDV INQIKIENPH LWDAQMKDEA TQMILQGTQL EIEYARDTMP RGVLGMNAAM MEDYLKFIAN RRLTQIGLKE EYPGTTNPFP WMSEIMDLKK EKNFFETRVI EYQTGGALSW D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccatgg gccgccgcaa caagatgacc ggcaccgccg agcagttcca gtacatcctc cgcgacgagt cgatgcacct gaacttcggt atcgacgtga tcaaccagat caagatcgag aacccgcacc tgtgggacgc ccagatgaag gacgaggcga cccagatgat cctccagggc acccagctgg agatcgaata cgcgcgcgac accatgccgc gcggggtgct gggcatgaac gcggcgatga tggaggacta cctgaagttc atcgccaacc ggcgcctgac ccagatcggc ctgaaggaag agtatccggg gaccaccaac ccgttcccgt ggatgtcgga gatcatggac ctgaagaagg agaagaactt cttcgagacg cgggttatcg agtaccagac gggcggagca ctgagctggg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat