SSGCID: Target Page

PsaeA.01416.a

Fructose-bisphosphate aldolase (FBP aldolase) (FBPA) (EC 4.1.2.13) (Fructose-1,6-bisphosphate aldolase)

CENTER ID:	PsaeA.01416.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01416.a.B1.GE38280	full length		1	354

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01416.a.B1.PW37607	full length		1	354

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.587
PGAT:	pseudomonas:PA0555
RefSeq:	NP_249246.1
UniProt:	Q9I5Y1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALISMRQML DHAAEFGYGV PAFNVNNLEQ MRAIMEAADK TDSPVIVQAS AGARKYAGAP FLRHLILAAI EEFPHIPVVM HQDHGTSPDV CQRSIQLGFS SVMMDGSLRE DGKTPADYDY NVRVTQQTVA FAHACGVSVE GELGCLGSLE TGMAGEEDGV GAEGVLDHSQ LLTDPEEAAD FVKKTKVDAL AIAIGTSHGA YKFTKPPTGD TLSIQRIKEI HARIPDTHLV MHGSSSVPQD WLAIINEYGG EIKETYGVPV EEIVEGIKYG VRKVNIDTDL RLASTGAIRR FLAQNPSEFD PRKYFSKTVE AMRDICIARY EAFGTAGNAS KIKPISLEGM FQRYARGELD PKVN

NT Sequence

atggcactca tcagcatgcg ccagatgctg gatcacgccg ccgaattcgg ctacggcgtg ccggccttca acgtcaacaa cctcgagcag atgcgcgcca tcatggaagc ggcggacaag accgattcgc ccgtgatcgt ccaggcttcc gccggtgctc gcaaatacgc cggcgcgccc ttcctgcgcc acctgatcct cgccgcgatc gaagaattcc cgcacatccc ggtggtcatg caccaggatc acggcaccag tcccgacgtc tgccagcgtt ccatccagct gggcttcagc tcggtgatga tggacggctc cctgcgtgaa gacggcaaga cccctgccga ctacgactac aacgtccgtg tgacccagca gaccgtggcg ttcgcccatg cctgcggcgt gtccgtggaa ggcgagctgg gctgcctggg ttccctggaa accggcatgg ccggcgagga agacggcgtc ggcgcggaag gcgtgctgga ccacagccag ttgctgaccg atccggaaga agccgccgac ttcgtgaaga agaccaaggt cgacgccctg gccatcgcca tcggcaccag ccacggcgcc tacaagttca ccaagccgcc gaccggggac accctgtcga tccagcgcat caaggaaatc cacgcgcgca tcccggacac ccacctggtg atgcacggct cttcctcggt accgcaggac tggctggcga tcatcaacga gtacggcggc gagatcaaag agacctacgg cgtgccggtc gaggaaatcg tcgagggcat caagtacggc gtgcgcaagg tgaacatcga caccgacctg cgcctggcct ccaccggtgc catccgccgc ttcctggcgc agaacccgag cgagttcgac ccgcgcaagt acttctccaa gaccgtcgag gccatgcgcg acatctgcat cgctcgctac gaagccttcg gcaccgccgg caacgcttcc aagatcaagc cgatctccct ggaaggcatg ttccagcgct acgctcgcgg cgagctggat ccgaaggtca actaa

Details for PsaeA.01416.a.B1.GE38280

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA LISMRQMLDH AAEFGYGVPA FNVNNLEQMR AIMEAADKTD SPVIVQASAG ARKYAGAPFL RHLILAAIEE FPHIPVVMHQ DHGTSPDVCQ RSIQLGFSSV MMDGSLREDG KTPADYDYNV RVTQQTVAFA HACGVSVEGE LGCLGSLETG MAGEEDGVGA EGVLDHSQLL TDPEEAADFV KKTKVDALAI AIGTSHGAYK FTKPPTGDTL SIQRIKEIHA RIPDTHLVMH GSSSVPQDWL AIINEYGGEI KETYGVPVEE IVEGIKYGVR KVNIDTDLRL ASTGAIRRFL AQNPSEFDPR KYFSKTVEAM RDICIARYEA FGTXHASRSX ISXXMFXATL AXXXSXVXXX SKXXS

Validated NT Sequence

annnnnnnnt nnantaattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggcactc atcagcatgc gccagatgct ggatcacgcc gccgaattcg gctacggcgt gccggccttc aacgtcaaca acctcgagca gatgcgcgcc atcatggaag cggcggacaa gaccgattcg cccgtgatcg tccaggcttc cgccggtgct cgcaaatacg ccggcgcgcc cttcctgcgc cacctgatcc tcgccgcgat cgaagaattc ccgcacatcc cggtggtcat gcaccaggat cacggcacca gtcccgacgt ctgccagcgt tccatccagc tgggcttcag ctcggtgatg atggacggct ccctgcgtga agacggcaag acccctgccg actacgacta caacgtccgt gtgacccagc agaccgtggc gttcgcccat gcctgcggcg tgtccgtgga aggcgagctg ggctgcctgg gttccctgga aaccggcatg gccggcgagg aagacggcgt cggcgcggaa ggcgtgctgg accacagcca gttgctgacc gatccggaag aagccgccga cttcgtgaag aagaccaagg tcgacgccct ggccatcgcc atcggcacca gccacggcgc ctacaagttc accaagccgc cgaccgggga caccctgtcg atccagcgca tcaaggaaat ccacgcgcgc atcccggaca cccacctggt gatgcacggc tcttcctcgg taccgcagga ctggctggcg atcatcaacg agtacggcgg cgagatcaaa gagacctacg gcgtgccggt cgaggaaatc gtcgagggca tcaagtacgg cgtgcgcaag gtgaacatcg acaccgacct gcgcctggcc tccaccggtg ccatccgccg cttcctggcg cagaacccga gcgagttcga cccgcgcaag tacttctcca agaccgtcga ggccatgcgc gacatctgca tcgctcgcta cgaagccttc ggcacgcngc acgcttccag atcancnatc tcctnnngca tgttcngcgc tacgctcgcg gnnanctgnt ccnangtcan taannngagt aagannngat ccgct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac tcatcagcat gcgccagatg ctggatcacg ccgccgaatt cggctacggc gtgccggcct tcaacgtcaa caacctcgag cagatgcgcg ccatcatgga agcggcggac aagaccgatt cgcccgtgat cgtccaggct tccgccggtg ctcgcaaata cgccggcgcg cccttcctgc gccacctgat cctcgccgcg atcgaagaat tcccgcacat cccggtggtc atgcaccagg atcacggcac cagtcccgac gtctgccagc gttccatcca gctgggcttc agctcggtga tgatggacgg ctccctgcgt gaagacggca agacccctgc cgactacgac tacaacgtcc gtgtgaccca gcagaccgtg gcgttcgccc atgcctgcgg cgtgtccgtg gaaggcgagc tgggctgcct gggttccctg gaaaccggca tggccggcga ggaagacggc gtcggcgcgg aaggcgtgct ggaccacagc cagttgctga ccgatccgga agaagccgcc gacttcgtga agaagaccaa ggtcgacgcc ctggccatcg ccatcggcac cagccacggc gcctacaagt tcaccaagcc gccgaccggg gacaccctgt cgatccagcg catcaaggaa atccacgcgc gcatcccgga cacccacctg gtgatgcacg gctcttcctc ggtaccgcag gactggctgg cgatcatcaa cgagtacggc ggcgagatca aagagaccta cggcgtgccg gtcgaggaaa tcgtcgaggg catcaagtac ggcgtgcgca aggtgaacat cgacaccgac ctgcgcctgg cctccaccgg tgccatccgc cgcttcctgg cgcagaaccc gagcgagttc gacccgcgca agtacttctc caagaccgtc gaggccatgc gcgacatctg catcgctcgc tacgaagcct tcggcaccgc cggcaacgct tccaagatca agccgatctc cctggaaggc atgttccagc gctacgctcg cggcgagctg gatccgaagg tcaactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for PsaeA.01416.a.B1.PW37607

PURIFICATION DATe:	12/1/2014
CONCENTRATION:	27mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	110µl
PERCENT IDENTITY:	92
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC TCATCAGCAT GCGCCAGATG CTGGATCACG CCGCCGAATT CGGCTACGGC GTGCCGGCCT TCAACGTCAA CAACCTCGAG CAGATGCGCG CCATCATGGA AGCGGCGGAC AAGACCGATT CGCCCGTGAT CGTCCAGGCT TCCGCCGGTG CTCGCAAATA CGCCGGCGCG CCCTTCCTGC GCCACCTGAT CCTCGCCGCG ATCGAAGAAT TCCCGCACAT CCCGGTGGTC ATGCACCAGG ATCACGGCAC CAGTCCCGAC GTCTGCCAGC GTTCCATCCA GCTGGGCTTC AGCTCGGTGA TGATGGACGG CTCCCTGCGT GAAGACGGCA AGACCCCTGC CGACTACGAC TACAACGTCC GTGTGACCCA GCAGACCGTG GCGTTCGCCC ATGCCTGCGG CGTGTCCGTG GAAGGCGAGC TGGGCTGCCT GGGTTCCCTG GAAACCGGCA TGGCCGGCGA GGAAGACGGC GTCGGCGCGG AAGGCGTGCT GGACCACAGC CAGTTGCTGA CCGATCCGGA AGAAGCCGCC GACTTCGTGA AGAAGACCAA GGTCGACGCC CTGGCCATCG CCATCGGCAC CAGCCACGGC GCCTACAAGT TCACCAAGCC GCCGACCGGG GACACCCTGT CGATCCAGCG CATCAAGGAA ATCCACGCGC GCATCCCGGA CACCCACCTG GTGATGCACG GCTCTTCCTC GGTACCGCAG GACTGGCTGG CGATCATCAA CGAGTACGGC GGCGAGATCA AAGAGACCTA CGGCGTGCCG GTCGAGGAAA TCGTCGAGGG CATCAAGTAC GGCGTGCGCA AGGTGAACAT CGACACCGAC CTGCGCCTGG CCTCCACCGG TGCCATCCGC CGCTTCCTGG CGCAGAACCC GAGCGAGTTC GACCCGCGCA AGTACTTCTC CAAGACCGTC GAGGCCATGC GCGACATCTG CATCGCTCGC TACGAAGCCT TCGGCACCGC CGGCAACGCT TCCAAGATCA AGCCGATCTC CCTGGAAGGC ATGTTCCAGC GCTACGCTCG CGGCGAGCTG GATCCGAAGG TCAACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT