PsaeA.01370.a

Phosphoglucosamine mutase (EC 5.4.2.10)

CENTER ID: PsaeA.01370.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.01370.a.B1.GE38279 full length 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.01370.a.B1.PW37560 full length 1 445

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4975
PGAT: pseudomonas:PA4749
RefSeq: NP_253437.1
UniProt: Q9HV50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKYFGTDG IRGRVGEFPI TPDFVLKLGW AVGMAFRRQG NCRVLIGKDT RSSGYMFESA FEAGLSASGA DTLLLGPMPT PGIAYLTRTF HAEAGVVISA SHNPHDDNGI KFFSGQGTKL PDDVELMIEE LLDAPMTVVE SARLGKVSRI NDAAGRYIEF CKSSVPTSTD FNGLKVVLDC ANGATYKIAP SVFRELGAEV TVLAASPNGL NINDKCGSTH LDGLQAAVVE HHADLGIAFD GDGDRVMMVD HTGAVVDGDE LLFLIARDLQ ESGRLQGGVV GTLMSNLGLE LALQELHIPF VRAKVGDRYV MAELLARNWM LGGENSGHIV CCQNTTTGDA IIAALQVLMA LKHRGQTLAE ARQGIRKCPQ VLINVRFKGE NDPLEHPSVK EASVRVTEQM GGRGRVLLRK SGTEPLVRVM VEGDEEASVR AHAEQLAKIV SEVCA
NT Sequence
atgagcagaa aatacttcgg gactgacggt atccgtggtc gtgttggcga gtttccgatt actccggact tcgttctcaa gctgggctgg gcggtgggca tggccttccg ccggcagggc aactgccggg tcctgatcgg caaggacacg cgcagctcgg gttacatgtt cgagtcggcc ttcgaggccg ggctctccgc ttccggcgcc gataccctgt tgctgggacc gatgcctacc cccggcatcg cctacctgac gcggactttc cacgccgagg ccggggtggt gatcagcgct tcgcacaatc cccacgacga caacggcatc aagttcttct ccgggcaggg gaccaagctg ccggacgacg tcgagctgat gatcgaggaa ctgctcgatg cgccgatgac cgtggtggag tccgcccgtc tcggcaaggt ttcgcggatc aatgacgcgg cggggcgcta catcgaattc tgcaagagca gcgtgccgac cagcaccgac ttcaacggtc tcaaggtcgt gctcgactgc gccaacggcg ctacctacaa gatcgctccc agcgtcttcc gggaactggg cgcggaagtg acggtgctgg cggccagccc gaatggcctg aacatcaacg acaagtgcgg ttccactcac ctggatggcc tgcaggctgc ggtggtcgag caccatgccg acctcggcat cgccttcgac ggcgacggcg accgggtgat gatggtcgac cataccggtg cggtcgtgga tggcgacgaa ctgcttttcc tcatcgcgcg cgacctgcag gaaagcgggc ggctgcaagg tggtgtggtc ggcaccctga tgagcaacct cggcctggag ctggctttgc aggaactgca tatcccgttc gtgcgggcga aggtcggcga tcgctatgtg atggccgaac tgctggctcg gaactggatg ctgggagggg agaattcggg gcatatcgtc tgctgccaga acaccacgac gggcgatgcc atcatcgcgg cgctacaggt gctgatggcg ctcaagcatc gcggccagac cctggcagag gcgcgccagg ggattcgcaa gtgcccccag gtgttgatca atgtgcgctt caagggcgaa aacgatccgc tcgagcaccc ctcggtgaag gaggccagcg tccgcgtcac cgagcaaatg ggcggtcgcg gtcgtgtcct gctgcgcaaa tccggcacag agccgttggt ccgggtgatg gtcgagggcg acgaggaagc tagcgttcgt gcccacgccg aacagctggc aaaaattgtt tctgaggtat gtgcttga
Details for PsaeA.01370.a.B1.GE38279
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MAFRRQGNCR VLIGKDTRSS GYMFESAFEA GLSASGADTL LLGPMPTPGI AYLTRTFHAE AGVVISASHN PHDDNGIKFF SGQGTKLPDD VELMIEELLD APMTVVESAR LGKVSRINDA AGRYIEFCKS SVPTSTDFNG LKVVLDCANG ATYKIAPSVF RELGAEVTVL AASPNGLNIN DKCGSTHLDG LQAAVVEHHA DLGIAFDGDG DRVMMVDHTG AVVDGDELLF LIARDLQESG RLQGGVVGTL MSNLGLELAL QELHIPFVRA KVGDRYVMAE LLARNWMLGG ENSGHIVCCQ NTTTGDAIIA ALQVLMALKH RGQTLAEARQ GIRKCPQVLI NVRFKGENDP LEHPSVKEAS VRVTEQMGGR GRVLLRKSGT EPLVRVMVEG DEEASVRAHA EQLA
Validated NT Sequence
ttgccagctg ttcggcgtgg gcacgaacgc tagcttcctc gtcgccctcg accatcaccc ggaccaacgg ctctgtgccg gatttgcgca gcaggacacg accgcgaccg cccatttgct cggtgacgcg gacgctggcc tccttcaccg aggggtgctc gagcggatcg ttttcgccct tgaagcgcac attgatcaac acctgggggc acttgcgaat cccctggcgc gcctctgcca gggtctggcc gcgatgcttg agcgccatca gcacctgtag cgccgcgatg atggcatcgc ccgtcgtggt gttctggcag cagacgatat gccccgaatt ctcccctccc agcatccagt tccgagccag cagttcggcc atcacatagc gatcgccgac cttcgcccgc acgaacggga tatgcagttc ctgcaaagcc agctccaggc cgaggttgct catcagggtg ccgaccacac caccttgcag ccgcccgctt tcctgcaggt cgcgcgcgat gaggaaaagc agttcgtcgc catccacgac cgcaccggta tggtcgacca tcatcacccg gtcgccgtcg ccgtcgaagg cgatgccgag gtcggcatgg tgctcgacca ccgcagcctg caggccatcc aggtgagtgg aaccgcactt gtcgttgatg ttcaggccat tcgggctggc cgccagcacc gtcacttccg cgcccagttc ccggaagacg ctgggagcga tcttgtaggt agcgccgttg gcgcagtcga gcacgacctt gagaccgttg aagtcggtgc tggtcggcac gctgctcttg cagaattcga tgtagcgccc cgccgcgtca ttgatccgcg aaaccttgcc gagacgggcg gactccacca cggtcatcgg cgcatcgagc agttcctcga tcatcagctc gacgtcgtcc ggcagcttgg tcccctgccc ggagaagaac ttgatgccgt tgtcgtcgtg gggattgtgc gaagcgctga tcaccacccc ggcctcggcg tggaaagtcc gcgtcaggta ggcgatgccg ggggtaggca tcggtcccag caacagggta tcggcgccgg aagcggagag cccggcctcg aaggccgact cgaacatgta acccgagctg cgcgtgtcct tgccgatcag gacccggcag ttgccctgcc ggcggaaggc catgcccacc gcccagccca gcttgagaac gaagtccgga gtaatcggaa actcgccaac acgaccacgg ataccgtcag tcccgaagta ttttctgc
Expected Protein Sequence
MAHHHHHHMS RKYFGTDGIR GRVGEFPITP DFVLKLGWAV GMAFRRQGNC RVLIGKDTRS SGYMFESAFE AGLSASGADT LLLGPMPTPG IAYLTRTFHA EAGVVISASH NPHDDNGIKF FSGQGTKLPD DVELMIEELL DAPMTVVESA RLGKVSRIND AAGRYIEFCK SSVPTSTDFN GLKVVLDCAN GATYKIAPSV FRELGAEVTV LAASPNGLNI NDKCGSTHLD GLQAAVVEHH ADLGIAFDGD GDRVMMVDHT GAVVDGDELL FLIARDLQES GRLQGGVVGT LMSNLGLELA LQELHIPFVR AKVGDRYVMA ELLARNWMLG GENSGHIVCC QNTTTGDAII AALQVLMALK HRGQTLAEAR QGIRKCPQVL INVRFKGEND PLEHPSVKEA SVRVTEQMGG RGRVLLRKSG TEPLVRVMVE GDEEASVRAH AEQLAKIVSE VCA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca gaaaatactt cgggactgac ggtatccgtg gtcgtgttgg cgagtttccg attactccgg acttcgttct caagctgggc tgggcggtgg gcatggcctt ccgccggcag ggcaactgcc gggtcctgat cggcaaggac acgcgcagct cgggttacat gttcgagtcg gccttcgagg ccgggctctc cgcttccggc gccgataccc tgttgctggg accgatgcct acccccggca tcgcctacct gacgcggact ttccacgccg aggccggggt ggtgatcagc gcttcgcaca atccccacga cgacaacggc atcaagttct tctccgggca ggggaccaag ctgccggacg acgtcgagct gatgatcgag gaactgctcg atgcgccgat gaccgtggtg gagtccgccc gtctcggcaa ggtttcgcgg atcaatgacg cggcggggcg ctacatcgaa ttctgcaaga gcagcgtgcc gaccagcacc gacttcaacg gtctcaaggt cgtgctcgac tgcgccaacg gcgctaccta caagatcgct cccagcgtct tccgggaact gggcgcggaa gtgacggtgc tggcggccag cccgaatggc ctgaacatca acgacaagtg cggttccact cacctggatg gcctgcaggc tgcggtggtc gagcaccatg ccgacctcgg catcgccttc gacggcgacg gcgaccgggt gatgatggtc gaccataccg gtgcggtcgt ggatggcgac gaactgcttt tcctcatcgc gcgcgacctg caggaaagcg ggcggctgca aggtggtgtg gtcggcaccc tgatgagcaa cctcggcctg gagctggctt tgcaggaact gcatatcccg ttcgtgcggg cgaaggtcgg cgatcgctat gtgatggccg aactgctggc tcggaactgg atgctgggag gggagaattc ggggcatatc gtctgctgcc agaacaccac gacgggcgat gccatcatcg cggcgctaca ggtgctgatg gcgctcaagc atcgcggcca gaccctggca gaggcgcgcc aggggattcg caagtgcccc caggtgttga tcaatgtgcg cttcaagggc gaaaacgatc cgctcgagca cccctcggtg aaggaggcca gcgtccgcgt caccgagcaa atgggcggtc gcggtcgtgt cctgctgcgc aaatccggca cagagccgtt ggtccgggtg atggtcgagg gcgacgagga agctagcgtt cgtgcccacg ccgaacagct ggcaaaaatt gtttctgagg tatgtgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.01370.a.B1.PW37560
PURIFICATION DATe: 9/16/2014
CONCENTRATION: 17mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MAFRRQGNCR VLIGKDTRSS GYMFESAFEA GLSASGADTL LLGPMPTPGI AYLTRTFHAE AGVVISASHN PHDDNGIKFF SGQGTKLPDD VELMIEELLD APMTVVESAR LGKVSRINDA AGRYIEFCKS SVPTSTDFNG LKVVLDCANG ATYKIAPSVF RELGAEVTVL AASPNGLNIN DKCGSTHLDG LQAAVVEHHA DLGIAFDGDG DRVMMVDHTG AVVDGDELLF LIARDLQESG RLQGGVVGTL MSNLGLELAL QELHIPFVRA KVGDRYVMAE LLARNWMLGG ENSGHIVCCQ NTTTGDAIIA ALQVLMALKH RGQTLAEARQ GIRKCPQVLI NVRFKGENDP LEHPSVKEAS VRVTEQMGGR GRVLLRKSGT EPLVRVMVEG DEEASVRAHA EQLA
Validated NT Sequence
ttgccagctg ttcggcgtgg gcacgaacgc tagcttcctc gtcgccctcg accatcaccc ggaccaacgg ctctgtgccg gatttgcgca gcaggacacg accgcgaccg cccatttgct cggtgacgcg gacgctggcc tccttcaccg aggggtgctc gagcggatcg ttttcgccct tgaagcgcac attgatcaac acctgggggc acttgcgaat cccctggcgc gcctctgcca gggtctggcc gcgatgcttg agcgccatca gcacctgtag cgccgcgatg atggcatcgc ccgtcgtggt gttctggcag cagacgatat gccccgaatt ctcccctccc agcatccagt tccgagccag cagttcggcc atcacatagc gatcgccgac cttcgcccgc acgaacggga tatgcagttc ctgcaaagcc agctccaggc cgaggttgct catcagggtg ccgaccacac caccttgcag ccgcccgctt tcctgcaggt cgcgcgcgat gaggaaaagc agttcgtcgc catccacgac cgcaccggta tggtcgacca tcatcacccg gtcgccgtcg ccgtcgaagg cgatgccgag gtcggcatgg tgctcgacca ccgcagcctg caggccatcc aggtgagtgg aaccgcactt gtcgttgatg ttcaggccat tcgggctggc cgccagcacc gtcacttccg cgcccagttc ccggaagacg ctgggagcga tcttgtaggt agcgccgttg gcgcagtcga gcacgacctt gagaccgttg aagtcggtgc tggtcggcac gctgctcttg cagaattcga tgtagcgccc cgccgcgtca ttgatccgcg aaaccttgcc gagacgggcg gactccacca cggtcatcgg cgcatcgagc agttcctcga tcatcagctc gacgtcgtcc ggcagcttgg tcccctgccc ggagaagaac ttgatgccgt tgtcgtcgtg gggattgtgc gaagcgctga tcaccacccc ggcctcggcg tggaaagtcc gcgtcaggta ggcgatgccg ggggtaggca tcggtcccag caacagggta tcggcgccgg aagcggagag cccggcctcg aaggccgact cgaacatgta acccgagctg cgcgtgtcct tgccgatcag gacccggcag ttgccctgcc ggcggaaggc catgcccacc gcccagccca gcttgagaac gaagtccgga gtaatcggaa actcgccaac acgaccacgg ataccgtcag tcccgaagta ttttctgc
Expressed Protein Sequence
MAHHHHHHMS RKYFGTDGIR GRVGEFPITP DFVLKLGWAV GMAFRRQGNC RVLIGKDTRS SGYMFESAFE AGLSASGADT LLLGPMPTPG IAYLTRTFHA EAGVVISASH NPHDDNGIKF FSGQGTKLPD DVELMIEELL DAPMTVVESA RLGKVSRIND AAGRYIEFCK SSVPTSTDFN GLKVVLDCAN GATYKIAPSV FRELGAEVTV LAASPNGLNI NDKCGSTHLD GLQAAVVEHH ADLGIAFDGD GDRVMMVDHT GAVVDGDELL FLIARDLQES GRLQGGVVGT LMSNLGLELA LQELHIPFVR AKVGDRYVMA ELLARNWMLG GENSGHIVCC QNTTTGDAII AALQVLMALK HRGQTLAEAR QGIRKCPQVL INVRFKGEND PLEHPSVKEA SVRVTEQMGG RGRVLLRKSG TEPLVRVMVE GDEEASVRAH AEQLAKIVSE VCA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA GAAAATACTT CGGGACTGAC GGTATCCGTG GTCGTGTTGG CGAGTTTCCG ATTACTCCGG ACTTCGTTCT CAAGCTGGGC TGGGCGGTGG GCATGGCCTT CCGCCGGCAG GGCAACTGCC GGGTCCTGAT CGGCAAGGAC ACGCGCAGCT CGGGTTACAT GTTCGAGTCG GCCTTCGAGG CCGGGCTCTC CGCTTCCGGC GCCGATACCC TGTTGCTGGG ACCGATGCCT ACCCCCGGCA TCGCCTACCT GACGCGGACT TTCCACGCCG AGGCCGGGGT GGTGATCAGC GCTTCGCACA ATCCCCACGA CGACAACGGC ATCAAGTTCT TCTCCGGGCA GGGGACCAAG CTGCCGGACG ACGTCGAGCT GATGATCGAG GAACTGCTCG ATGCGCCGAT GACCGTGGTG GAGTCCGCCC GTCTCGGCAA GGTTTCGCGG ATCAATGACG CGGCGGGGCG CTACATCGAA TTCTGCAAGA GCAGCGTGCC GACCAGCACC GACTTCAACG GTCTCAAGGT CGTGCTCGAC TGCGCCAACG GCGCTACCTA CAAGATCGCT CCCAGCGTCT TCCGGGAACT GGGCGCGGAA GTGACGGTGC TGGCGGCCAG CCCGAATGGC CTGAACATCA ACGACAAGTG CGGTTCCACT CACCTGGATG GCCTGCAGGC TGCGGTGGTC GAGCACCATG CCGACCTCGG CATCGCCTTC GACGGCGACG GCGACCGGGT GATGATGGTC GACCATACCG GTGCGGTCGT GGATGGCGAC GAACTGCTTT TCCTCATCGC GCGCGACCTG CAGGAAAGCG GGCGGCTGCA AGGTGGTGTG GTCGGCACCC TGATGAGCAA CCTCGGCCTG GAGCTGGCTT TGCAGGAACT GCATATCCCG TTCGTGCGGG CGAAGGTCGG CGATCGCTAT GTGATGGCCG AACTGCTGGC TCGGAACTGG ATGCTGGGAG GGGAGAATTC GGGGCATATC GTCTGCTGCC AGAACACCAC GACGGGCGAT GCCATCATCG CGGCGCTACA GGTGCTGATG GCGCTCAAGC ATCGCGGCCA GACCCTGGCA GAGGCGCGCC AGGGGATTCG CAAGTGCCCC CAGGTGTTGA TCAATGTGCG CTTCAAGGGC GAAAACGATC CGCTCGAGCA CCCCTCGGTG AAGGAGGCCA GCGTCCGCGT CACCGAGCAA ATGGGCGGTC GCGGTCGTGT CCTGCTGCGC AAATCCGGCA CAGAGCCGTT GGTCCGGGTG ATGGTCGAGG GCGACGAGGA AGCTAGCGTT CGTGCCCACG CCGAACAGCT GGCAAAAATT GTTTCTGAGG TATGTGCTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT