PsaeA.01348.a
Glutamate--tRNA ligase (EC 6.1.1.17) (Glutamyl-tRNA synthetase) (GluRS)
| CENTER ID: | PsaeA.01348.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01348.a.B1.GE38278 | full length | 1 | 494 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01348.a.B1.PS02214 | full length | 1 | 494 |
Structures
5TGT
DEPOSITED: 9/28/2016
DETERMINATION: XRay
CLONE: PsaeA.01348.a.B1.GE38278
PROTEIN: PsaeA.01348.a.B1.PS02214
8VC5
DEPOSITED: 12/13/2023
DETERMINATION: XRay
CLONE: PsaeA.01348.a.B1.GE38278
PROTEIN: PsaeA.01348.a.B1.PW37887
Publications by SSGCID
Architecture of Pseudomonas aeruginosa glutamyl tRNA synthetase defines new subfamily of class Ib aminoacyl tRNA synthetases
Abramov A, Choi R, Edwards TE, Hewitt SN, Lorimer D, Myler PJ, Phan I, Staker BL, Subramanian S, Dranow D, Bullard JM, Mayclin SJ, Wower IK, Battaile KP, Seibold S, Lovell S, DeRocher AE, Soe AC, Tsutakawa SE, Wower J
Proc. Natl. Acad. Sci. U.S.A. - 2025
volume 122, issue 19, pages e2504757122
PMID: 40343997; PMCID: PMC12088422
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|208964.12.peg.3286 |
| PGAT: | pseudomonas:PA3134 |
| RefSeq: | NP_251824.1 |
| UniProt: | Q9XCL6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTVRTRIAP SPTGDPHVGT AYIALFNLCF ARQHGGQFIL RIEDTDQLRS TRESEQQIYD ALRWLGIEWD EGPDVGGPHG PYRQSERGHI YKKYSDELVE KGHAFTCFCT PERLDAVRAE QMARKETPRY DGHCMHLPKD EVQRRLAAGE SHVTRMKVPT EGVCVVPDML RGDVEIPWDR MDMQVLMKAD GLPTYFLANV VDDHLMGITH VLRGEEWLPS APKLIKLYEY FGWEQPQLCY MPLLRNPDKS KLSKRKNPTS ITFYERMGYL PQALLNYLGR MGWSMPDERE KFTLAEMIEH FDLSRVSLGG PIFDLEKLSW LNGQWIREQS VEEFAREVQK WALNPEYLMK IAPHVQGRVE NFSQIAPLAG FFFSGGVPLD ASLFEHKKLD PTQVRQVLQL VLWKLESLRQ WEKERITGCI QAVAEHLQLK LRDVMPLMFP AITGHASSVS VLDAMEILGA DLSRYRLRQA LELLGGASKK ETKEWEKIRD AIPG
NT Sequence
atgaccactg ttcgtactcg catcgccccg tcgccgacgg gcgacccgca cgtcggtacc gcatacatcg cgctgttcaa cctgtgcttc gcgcgtcagc acggcggtca gttcatcctg cgcatcgagg acaccgacca gctgcgttcg acgcgggagt ccgagcagca gatctacgac gccctgcgct ggctcggcat cgaatgggac gagggcccgg acgtcggcgg cccgcatggc ccgtaccggc agagcgagcg cgggcatatc tacaagaagt attcggacga actggtcgag aagggccatg ccttcacctg cttctgcacg cccgagcgcc tcgatgcggt tcgcgccgag cagatggcgc gtaaggaaac cccgcgctac gacggccact gcatgcacct gccgaaggat gaggtgcagc gccgcctcgc cgccggcgag tcccacgtca cccgcatgaa ggtgccgacc gagggcgtct gcgtggtgcc ggacatgctc cgcggcgatg tcgagattcc gtgggatcgc atggacatgc aggtgctgat gaaggccgac ggcctgccca cctacttcct cgccaacgtg gtcgacgacc acctgatggg catcacccac gtcctgcgcg gcgaagagtg gctgccgtcg gcgcccaagc tgatcaagct ctacgagtat ttcggctggg agcagccgca gctgtgctac atgccactgc tgcgcaaccc ggacaagagc aagctgtcca agcgcaagaa cccgacctct atcaccttct acgagcgcat gggctacctg ccgcaggcgc tgctcaacta cctcgggcgg atgggctggt cgatgccgga cgagcgcgag aaattcaccc tcgccgagat gatcgagcat ttcgacctgt cgcgcgtctc cctgggcgga ccgatcttcg acctggagaa gctctcctgg ctgaacgggc agtggatccg cgagcagtcc gtggaggagt tcgcccgtga ggtgcagaag tgggcgttga atcccgaata cctgatgaag atcgctccgc acgtgcaggg acgggtggag aacttcagcc agatcgcgcc gctggccgga ttcttcttca gcggcggggt gccgctggac gcttcgctgt tcgaacacaa gaagctcgat ccgacccagg tccgccaggt cctgcaactg gtgctgtgga aactcgaatc cctgcgccag tgggagaagg aacgcatcac cggctgcatc caggctgtcg ccgaacacct ccagctcaag ctgcgcgacg tgatgccgct gatgttcccg gccatcaccg ggcatgccag ctcggtttcg gtgctggacg ccatggagat ccttggcgcc gacctgtcgc gttatcgctt gcgccaggcc ctggagctgc tgggcggggc gtcgaaaaag gaaaccaagg agtgggagaa gatccgcgac gccattcccg gctga
Details for PsaeA.01348.a.B1.GE38278
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT TVRTRIAPSP TGDPHVGTAY IALFNLCFAR QHGGQFILRI EDTDQLRSTR ESEQQIYDAL RWLGIEWDEG PDVGGPHGPY RQSERGHIYK KYSDELVEKG HAFTCFCTPE RLDAVRAEQM ARKETPRYDG HCMHLPKDEV QRRLAAGESH VTRMKVPTEG VCVVPDMLRG DVEIPWDRMD MQVLMKADGL PTYFLANVVD DHLMGITHVL RGEEWLPSAP KLIKLYEYFG WEQPQLCYMP LLRNPDKSKL SKRKNPTSIT FYERMGYLPQ ALLNYLGRMG WSMPDEREKF TLAEMIEHFD LSRVSLGGPI FDLEKLSWLN GQWIREQSVE EFAREVQKWA LNPEYLMKIA PHVQGRVENF SQIAPLAGFF FSGGVPLDAS LFEHKKLDPT QVRQVLQLVL WKLESLRQWE KERITGCIQA VAEHLQLKLR DVMPLMFPAI TGHASSVSVL DAMEILGADL SRYRLRQALE LLGGASKKET KEWEKIRDAI PG
Validated NT Sequence
atggctcacc accaccacca ccatatgacc actgttcgta ctcgcatcgc cccgtcgccg acgggcgacc cgcacgtcgg taccgcatac atcgcgctgt tcaacctgtg cttcgcgcgt cagcacggcg gtcagttcat cctgcgcatc gaggacaccg accagctgcg ttcgacgcgg gagtccgagc agcagatcta cgacgccctg cgctggctcg gcatcgaatg ggacgagggc ccggacgtcg gcggcccgca tggcccgtac cggcagagcg agcgcgggca tatctacaag aagtattcgg acgaactggt cgagaagggc catgccttca cctgcttctg cacgcccgag cgcctcgatg cggttcgcgc cgagcagatg gcgcgtaagg aaaccccgcg ctacgacggc cactgcatgc acctgccgaa ggatgaggtg cagcgccgcc tcgccgccgg cgagtcccac gtcacccgca tgaaggtgcc gaccgagggc gtctgcgtgg tgccggacat gctccgcggc gatgtcgaga ttccgtggga tcgcat-gga catgcaggtg ctgatgaagg ccgacggcct gcccacctac ttcctcgcca acgtggtcga cgaccacctg atgggcatca cccacgtcct gcgcggcgaa gagtggctgc cgtcggcgcc caagctgatc aagctctacg agtatttcgg ctgggagcag ccgcagctgt gctacatgcc actgctgcgc aacccggaca agagcaagct gtccaagcgc aagaacccga cctctatcac cttctacgag cgcatgggct acctgccgca ggcgctgctc aactacctcg ggcggatggg ctggtcgatg ccggacgagc gcgagaaatt caccctcgcc gagatgatcg agcatttcga cctgtcgcgc gtctccctgg gcggaccgat cttcgacctg gagaagctct cctggctgaa cgggcagtgg atccgcgagc agtccgtgga ggagttcgcc cgtgaggtgc agaagtgggc gttgaatccc gaatacctga tgaagatcgc tccgcacgtg cagggacggg tggagaactt cagccagatc gcgccgctgg ccggattctt cttcagcggc ggggtgccgc tggacgcttc gctgttcgaa cacaagaagc tcgatccgac ccaggtccgc caggtcctgc aactggtgct gtggaaactc gaatccctgc gccagtggga gaaggaacgc atcaccggct gcatccaggc tgtcgccgaa cacctccagc tcaagctgcg cgacgtgatg ccgctgatgt tcccggccat caccgggcat gccagctcgg tttcggtgct ggacgccatg gagatccttg gcgccgacct gtcgcgttat cgcttgcgcc aggccctgga gctgctgggc ggggcgtcga aaaaggaaac caaggagtgg gagaagatcc gcgacgccat tcccggctaa
Expected Protein Sequence
MAHHHHHHMT TVRTRIAPSP TGDPHVGTAY IALFNLCFAR QHGGQFILRI EDTDQLRSTR ESEQQIYDAL RWLGIEWDEG PDVGGPHGPY RQSERGHIYK KYSDELVEKG HAFTCFCTPE RLDAVRAEQM ARKETPRYDG HCMHLPKDEV QRRLAAGESH VTRMKVPTEG VCVVPDMLRG DVEIPWDRMD MQVLMKADGL PTYFLANVVD DHLMGITHVL RGEEWLPSAP KLIKLYEYFG WEQPQLCYMP LLRNPDKSKL SKRKNPTSIT FYERMGYLPQ ALLNYLGRMG WSMPDEREKF TLAEMIEHFD LSRVSLGGPI FDLEKLSWLN GQWIREQSVE EFAREVQKWA LNPEYLMKIA PHVQGRVENF SQIAPLAGFF FSGGVPLDAS LFEHKKLDPT QVRQVLQLVL WKLESLRQWE KERITGCIQA VAEHLQLKLR DVMPLMFPAI TGHASSVSVL DAMEILGADL SRYRLRQALE LLGGASKKET KEWEKIRDAI PG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca ctgttcgtac tcgcatcgcc ccgtcgccga cgggcgaccc gcacgtcggt accgcataca tcgcgctgtt caacctgtgc ttcgcgcgtc agcacggcgg tcagttcatc ctgcgcatcg aggacaccga ccagctgcgt tcgacgcggg agtccgagca gcagatctac gacgccctgc gctggctcgg catcgaatgg gacgagggcc cggacgtcgg cggcccgcat ggcccgtacc ggcagagcga gcgcgggcat atctacaaga agtattcgga cgaactggtc gagaagggcc atgccttcac ctgcttctgc acgcccgagc gcctcgatgc ggttcgcgcc gagcagatgg cgcgtaagga aaccccgcgc tacgacggcc actgcatgca cctgccgaag gatgaggtgc agcgccgcct cgccgccggc gagtcccacg tcacccgcat gaaggtgccg accgagggcg tctgcgtggt gccggacatg ctccgcggcg atgtcgagat tccgtgggat cgcatggaca tgcaggtgct gatgaaggcc gacggcctgc ccacctactt cctcgccaac gtggtcgacg accacctgat gggcatcacc cacgtcctgc gcggcgaaga gtggctgccg tcggcgccca agctgatcaa gctctacgag tatttcggct gggagcagcc gcagctgtgc tacatgccac tgctgcgcaa cccggacaag agcaagctgt ccaagcgcaa gaacccgacc tctatcacct tctacgagcg catgggctac ctgccgcagg cgctgctcaa ctacctcggg cggatgggct ggtcgatgcc ggacgagcgc gagaaattca ccctcgccga gatgatcgag catttcgacc tgtcgcgcgt ctccctgggc ggaccgatct tcgacctgga gaagctctcc tggctgaacg ggcagtggat ccgcgagcag tccgtggagg agttcgcccg tgaggtgcag aagtgggcgt tgaatcccga atacctgatg aagatcgctc cgcacgtgca gggacgggtg gagaacttca gccagatcgc gccgctggcc ggattcttct tcagcggcgg ggtgccgctg gacgcttcgc tgttcgaaca caagaagctc gatccgaccc aggtccgcca ggtcctgcaa ctggtgctgt ggaaactcga atccctgcgc cagtgggaga aggaacgcat caccggctgc atccaggctg tcgccgaaca cctccagctc aagctgcgcg acgtgatgcc gctgatgttc ccggccatca ccgggcatgc cagctcggtt tcggtgctgg acgccatgga gatccttggc gccgacctgt cgcgttatcg cttgcgccag gccctggagc tgctgggcgg ggcgtcgaaa aaggaaacca aggagtggga gaagatccgc gacgccattc ccggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.01348.a.B1.PS02214
| PURIFICATION DATe: | 11/11/2014 |
| CONCENTRATION: | 48.2mg/ml |
| OBSERVED MW: | 54kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TVRTRIAPSP TGDPHVGTAY IALFNLCFAR QHGGQFILRI EDTDQLRSTR ESEQQIYDAL RWLGIEWDEG PDVGGPHGPY RQSERGHIYK KYSDELVEKG HAFTCFCTPE RLDAVRAEQM ARKETPRYDG HCMHLPKDEV QRRLAAGESH VTRMKVPTEG VCVVPDMLRG DVEIPWDRMD MQVLMKADGL PTYFLANVVD DHLMGITHVL RGEEWLPSAP KLIKLYEYFG WEQPQLCYMP LLRNPDKSKL SKRKNPTSIT FYERMGYLPQ ALLNYLGRMG WSMPDEREKF TLAEMIEHFD LSRVSLGGPI FDLEKLSWLN GQWIREQSVE EFAREVQKWA LNPEYLMKIA PHVQGRVENF SQIAPLAGFF FSGGVPLDAS LFEHKKLDPT QVRQVLQLVL WKLESLRQWE KERITGCIQA VAEHLQLKLR DVMPLMFPAI TGHASSVSVL DAMEILGADL SRYRLRQALE LLGGASKKET KEWEKIRDAI PG
Validated NT Sequence
atggctcacc accaccacca ccatatgacc actgttcgta ctcgcatcgc cccgtcgccg acgggcgacc cgcacgtcgg taccgcatac atcgcgctgt tcaacctgtg cttcgcgcgt cagcacggcg gtcagttcat cctgcgcatc gaggacaccg accagctgcg ttcgacgcgg gagtccgagc agcagatcta cgacgccctg cgctggctcg gcatcgaatg ggacgagggc ccggacgtcg gcggcccgca tggcccgtac cggcagagcg agcgcgggca tatctacaag aagtattcgg acgaactggt cgagaagggc catgccttca cctgcttctg cacgcccgag cgcctcgatg cggttcgcgc cgagcagatg gcgcgtaagg aaaccccgcg ctacgacggc cactgcatgc acctgccgaa ggatgaggtg cagcgccgcc tcgccgccgg cgagtcccac gtcacccgca tgaaggtgcc gaccgagggc gtctgcgtgg tgccggacat gctccgcggc gatgtcgaga ttccgtggga tcgcat-gga catgcaggtg ctgatgaagg ccgacggcct gcccacctac ttcctcgcca acgtggtcga cgaccacctg atgggcatca cccacgtcct gcgcggcgaa gagtggctgc cgtcggcgcc caagctgatc aagctctacg agtatttcgg ctgggagcag ccgcagctgt gctacatgcc actgctgcgc aacccggaca agagcaagct gtccaagcgc aagaacccga cctctatcac cttctacgag cgcatgggct acctgccgca ggcgctgctc aactacctcg ggcggatggg ctggtcgatg ccggacgagc gcgagaaatt caccctcgcc gagatgatcg agcatttcga cctgtcgcgc gtctccctgg gcggaccgat cttcgacctg gagaagctct cctggctgaa cgggcagtgg atccgcgagc agtccgtgga ggagttcgcc cgtgaggtgc agaagtgggc gttgaatccc gaatacctga tgaagatcgc tccgcacgtg cagggacggg tggagaactt cagccagatc gcgccgctgg ccggattctt cttcagcggc ggggtgccgc tggacgcttc gctgttcgaa cacaagaagc tcgatccgac ccaggtccgc caggtcctgc aactggtgct gtggaaactc gaatccctgc gccagtggga gaaggaacgc atcaccggct gcatccaggc tgtcgccgaa cacctccagc tcaagctgcg cgacgtgatg ccgctgatgt tcccggccat caccgggcat gccagctcgg tttcggtgct ggacgccatg gagatccttg gcgccgacct gtcgcgttat cgcttgcgcc aggccctgga gctgctgggc ggggcgtcga aaaaggaaac caaggagtgg gagaagatcc gcgacgccat tcccggctaa
Expressed Protein Sequence
MAHHHHHHMT TVRTRIAPSP TGDPHVGTAY IALFNLCFAR QHGGQFILRI EDTDQLRSTR ESEQQIYDAL RWLGIEWDEG PDVGGPHGPY RQSERGHIYK KYSDELVEKG HAFTCFCTPE RLDAVRAEQM ARKETPRYDG HCMHLPKDEV QRRLAAGESH VTRMKVPTEG VCVVPDMLRG DVEIPWDRMD MQVLMKADGL PTYFLANVVD DHLMGITHVL RGEEWLPSAP KLIKLYEYFG WEQPQLCYMP LLRNPDKSKL SKRKNPTSIT FYERMGYLPQ ALLNYLGRMG WSMPDEREKF TLAEMIEHFD LSRVSLGGPI FDLEKLSWLN GQWIREQSVE EFAREVQKWA LNPEYLMKIA PHVQGRVENF SQIAPLAGFF FSGGVPLDAS LFEHKKLDPT QVRQVLQLVL WKLESLRQWE KERITGCIQA VAEHLQLKLR DVMPLMFPAI TGHASSVSVL DAMEILGADL SRYRLRQALE LLGGASKKET KEWEKIRDAI PG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCA CTGTTCGTAC TCGCATCGCC CCGTCGCCGA CGGGCGACCC GCACGTCGGT ACCGCATACA TCGCGCTGTT CAACCTGTGC TTCGCGCGTC AGCACGGCGG TCAGTTCATC CTGCGCATCG AGGACACCGA CCAGCTGCGT TCGACGCGGG AGTCCGAGCA GCAGATCTAC GACGCCCTGC GCTGGCTCGG CATCGAATGG GACGAGGGCC CGGACGTCGG CGGCCCGCAT GGCCCGTACC GGCAGAGCGA GCGCGGGCAT ATCTACAAGA AGTATTCGGA CGAACTGGTC GAGAAGGGCC ATGCCTTCAC CTGCTTCTGC ACGCCCGAGC GCCTCGATGC GGTTCGCGCC GAGCAGATGG CGCGTAAGGA AACCCCGCGC TACGACGGCC ACTGCATGCA CCTGCCGAAG GATGAGGTGC AGCGCCGCCT CGCCGCCGGC GAGTCCCACG TCACCCGCAT GAAGGTGCCG ACCGAGGGCG TCTGCGTGGT GCCGGACATG CTCCGCGGCG ATGTCGAGAT TCCGTGGGAT CGCATGGACA TGCAGGTGCT GATGAAGGCC GACGGCCTGC CCACCTACTT CCTCGCCAAC GTGGTCGACG ACCACCTGAT GGGCATCACC CACGTCCTGC GCGGCGAAGA GTGGCTGCCG TCGGCGCCCA AGCTGATCAA GCTCTACGAG TATTTCGGCT GGGAGCAGCC GCAGCTGTGC TACATGCCAC TGCTGCGCAA CCCGGACAAG AGCAAGCTGT CCAAGCGCAA GAACCCGACC TCTATCACCT TCTACGAGCG CATGGGCTAC CTGCCGCAGG CGCTGCTCAA CTACCTCGGG CGGATGGGCT GGTCGATGCC GGACGAGCGC GAGAAATTCA CCCTCGCCGA GATGATCGAG CATTTCGACC TGTCGCGCGT CTCCCTGGGC GGACCGATCT TCGACCTGGA GAAGCTCTCC TGGCTGAACG GGCAGTGGAT CCGCGAGCAG TCCGTGGAGG AGTTCGCCCG TGAGGTGCAG AAGTGGGCGT TGAATCCCGA ATACCTGATG AAGATCGCTC CGCACGTGCA GGGACGGGTG GAGAACTTCA GCCAGATCGC GCCGCTGGCC GGATTCTTCT TCAGCGGCGG GGTGCCGCTG GACGCTTCGC TGTTCGAACA CAAGAAGCTC GATCCGACCC AGGTCCGCCA GGTCCTGCAA CTGGTGCTGT GGAAACTCGA ATCCCTGCGC CAGTGGGAGA AGGAACGCAT CACCGGCTGC ATCCAGGCTG TCGCCGAACA CCTCCAGCTC AAGCTGCGCG ACGTGATGCC GCTGATGTTC CCGGCCATCA CCGGGCATGC CAGCTCGGTT TCGGTGCTGG ACGCCATGGA GATCCTTGGC GCCGACCTGT CGCGTTATCG CTTGCGCCAG GCCCTGGAGC TGCTGGGCGG GGCGTCGAAA AAGGAAACCA AGGAGTGGGA GAAGATCCGC GACGCCATTC CCGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT