PsaeA.01328.a
3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38) (CMP-2-keto-3-deoxyoctulosonic acid synthase) (CKS) (CMP-KDO synthase)
| CENTER ID: | PsaeA.01328.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01328.a.B1.GE38276 | full length | 1 | 254 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01328.a.B1.PW37559 | full length | 1 | 254 |
Structures
4XWI
DEPOSITED: 1/29/2015
DETERMINATION: XRay
CLONE: PsaeA.01328.a.B1.GE38276
PROTEIN: PsaeA.01328.a.B1.PW37559
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.3126 |
| PGAT: | pseudomonas:PA2979 |
| RefSeq: | NP_251669.1 |
| UniProt: | Q9HZM5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQAFTVVIP ARYASTRLPG KPLQDIAGQP MIQRVWNQAR KSAASRVVVA TDDERILAAC QGFGAEALLT RAEHNSGTDR LEEVASRLGL ASDAIVVNVQ GDEPLIPPAL IDQVAANLAA HPEAAIATLA EPIHEVSALF NPNVVKVATD IDGLALTFSR APLPWARDAF ARDRDSLPEG VPYRRHIGIY AYRVGFLADF VAWGPCWLEN AESLEQLRAL WHGVRIHVAD ARENMLPGVD TPEDLERVRR VLGG
NT Sequence
atgacccagg cgttcactgt cgtcattccc gcccgctacg cctccactcg cctgcccggc aagccattgc aggatatcgc cggccagcca atgatccagc gcgtctggaa ccaggcccgc aagagcgccg ccagccgcgt ggtcgtcgcc accgacgacg aacgcatcct ggcggcgtgc caggggttcg gcgccgaggc gctgctgacg cgtgccgagc acaactccgg caccgatcgc ctggaggaag tcgcttcccg gctcggcctg gccagcgatg ccatcgtggt caacgtccag ggcgacgaac cgctgatccc cccggcgctc atcgaccagg tggcggccaa cctggcggcg cacccggaag ctgccatcgc taccctggcc gagccgatcc atgaggtctc cgcgctgttc aatccgaacg tggtcaaggt cgccaccgac atcgacggcc tggccctgac tttcagtcgt gcgcccttgc cctgggctcg cgacgccttc gcccgcgatc gcgacagcct gccggagggc gtgccctatc gtcgccacat cggcatctac gcctatcgcg tgggtttcct tgccgatttc gtcgcctggg gaccgtgctg gctggagaac gccgaaagcc ttgagcaatt gcgcgcgctc tggcacgggg tgcgaatcca tgtggccgat gcccgcgaaa acatgcttcc gggcgtggat acgccggaag acctcgaacg cgttcgccgc gtgttggggg gctga
Details for PsaeA.01328.a.B1.GE38276
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT QAFTVVIPAR YASTRLPGKP LQDIAGQPMI QRVWNQARKS AASRVVVATD DERILAACQG FGAEALLTRA EHNSGTDRLE EVASRLGLAS DAIVVNVQGD EPLIPPALID QVAANLAAHP EAAIATLAEP IHEVSALFNP NVVKVATDID GLALTFSRAP LPWARDAFAR DRDSLPEGVP YRRHIGIYAY RVGFLADFVA WGPCWLENAE SLEQLRALWH GVRIHVADAR ENMLPGVDTP EDLERVRRVL GG
Validated NT Sequence
atcttactca cttagccccc caacacgcgg cgaacgcgtt cgaggtcttc cggcgtatcc acgcccggaa gcatgttttc gcgggcatcg gccacatgga ttcgcacccc gtgccagagc gcgcgcaatt gctcaaggct ttcggcgttc tccagccagc acggtcccca ggcgacgaaa tcggcaagga aacccacgcg ataggcgtag atgccgatgt ggcgacgata gggcacgccc tccggcaggc tgtcgcgatc gcgggcgaag gcgtcgcgag cccagggcaa gggcgcacga ctgaaagtca gggccaggcc gtcgatgtcg gtggcgacct tgaccacgtt cggattgaac agcgcggaga cctcatggat cggctcggcc agggtagcga tggcagcttc cgggtgcgcc gccaggttgg ccgccacctg gtcgatgagc gccgggggga tcagcggttc gtcgccctgg acgttgacca cgatggcatc gctggccagg ccgagccggg aagcgacttc ctccaggcga tcggtgccgg agttgtgctc ggcacgcgtc agcagcgcct cggcgccgaa cccctggcac gccgccagga tgcgttcgtc gtcggtggcg acgaccacgc ggctggcggc gctcttgcgg gcctggttcc agacgcgctg gatcattggc tggccggcga tatcctgcaa tggcttgccg ggcaggcgag tggaggcgta gcgggcggga atgacgacag tgaacgcctg ggtcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT QAFTVVIPAR YASTRLPGKP LQDIAGQPMI QRVWNQARKS AASRVVVATD DERILAACQG FGAEALLTRA EHNSGTDRLE EVASRLGLAS DAIVVNVQGD EPLIPPALID QVAANLAAHP EAAIATLAEP IHEVSALFNP NVVKVATDID GLALTFSRAP LPWARDAFAR DRDSLPEGVP YRRHIGIYAY RVGFLADFVA WGPCWLENAE SLEQLRALWH GVRIHVADAR ENMLPGVDTP EDLERVRRVL GG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aggcgttcac tgtcgtcatt cccgcccgct acgcctccac tcgcctgccc ggcaagccat tgcaggatat cgccggccag ccaatgatcc agcgcgtctg gaaccaggcc cgcaagagcg ccgccagccg cgtggtcgtc gccaccgacg acgaacgcat cctggcggcg tgccaggggt tcggcgccga ggcgctgctg acgcgtgccg agcacaactc cggcaccgat cgcctggagg aagtcgcttc ccggctcggc ctggccagcg atgccatcgt ggtcaacgtc cagggcgacg aaccgctgat ccccccggcg ctcatcgacc aggtggcggc caacctggcg gcgcacccgg aagctgccat cgctaccctg gccgagccga tccatgaggt ctccgcgctg ttcaatccga acgtggtcaa ggtcgccacc gacatcgacg gcctggccct gactttcagt cgtgcgccct tgccctgggc tcgcgacgcc ttcgcccgcg atcgcgacag cctgccggag ggcgtgccct atcgtcgcca catcggcatc tacgcctatc gcgtgggttt ccttgccgat ttcgtcgcct ggggaccgtg ctggctggag aacgccgaaa gccttgagca attgcgcgcg ctctggcacg gggtgcgaat ccatgtggcc gatgcccgcg aaaacatgct tccgggcgtg gatacgccgg aagacctcga acgcgttcgc cgcgtgttgg ggggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.01328.a.B1.PW37559
| PURIFICATION DATe: | 9/16/2014 |
| CONCENTRATION: | 25mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QAFTVVIPAR YASTRLPGKP LQDIAGQPMI QRVWNQARKS AASRVVVATD DERILAACQG FGAEALLTRA EHNSGTDRLE EVASRLGLAS DAIVVNVQGD EPLIPPALID QVAANLAAHP EAAIATLAEP IHEVSALFNP NVVKVATDID GLALTFSRAP LPWARDAFAR DRDSLPEGVP YRRHIGIYAY RVGFLADFVA WGPCWLENAE SLEQLRALWH GVRIHVADAR ENMLPGVDTP EDLERVRRVL GG
Validated NT Sequence
atcttactca cttagccccc caacacgcgg cgaacgcgtt cgaggtcttc cggcgtatcc acgcccggaa gcatgttttc gcgggcatcg gccacatgga ttcgcacccc gtgccagagc gcgcgcaatt gctcaaggct ttcggcgttc tccagccagc acggtcccca ggcgacgaaa tcggcaagga aacccacgcg ataggcgtag atgccgatgt ggcgacgata gggcacgccc tccggcaggc tgtcgcgatc gcgggcgaag gcgtcgcgag cccagggcaa gggcgcacga ctgaaagtca gggccaggcc gtcgatgtcg gtggcgacct tgaccacgtt cggattgaac agcgcggaga cctcatggat cggctcggcc agggtagcga tggcagcttc cgggtgcgcc gccaggttgg ccgccacctg gtcgatgagc gccgggggga tcagcggttc gtcgccctgg acgttgacca cgatggcatc gctggccagg ccgagccggg aagcgacttc ctccaggcga tcggtgccgg agttgtgctc ggcacgcgtc agcagcgcct cggcgccgaa cccctggcac gccgccagga tgcgttcgtc gtcggtggcg acgaccacgc ggctggcggc gctcttgcgg gcctggttcc agacgcgctg gatcattggc tggccggcga tatcctgcaa tggcttgccg ggcaggcgag tggaggcgta gcgggcggga atgacgacag tgaacgcctg ggtcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMT QAFTVVIPAR YASTRLPGKP LQDIAGQPMI QRVWNQARKS AASRVVVATD DERILAACQG FGAEALLTRA EHNSGTDRLE EVASRLGLAS DAIVVNVQGD EPLIPPALID QVAANLAAHP EAAIATLAEP IHEVSALFNP NVVKVATDID GLALTFSRAP LPWARDAFAR DRDSLPEGVP YRRHIGIYAY RVGFLADFVA WGPCWLENAE SLEQLRALWH GVRIHVADAR ENMLPGVDTP EDLERVRRVL GG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AGGCGTTCAC TGTCGTCATT CCCGCCCGCT ACGCCTCCAC TCGCCTGCCC GGCAAGCCAT TGCAGGATAT CGCCGGCCAG CCAATGATCC AGCGCGTCTG GAACCAGGCC CGCAAGAGCG CCGCCAGCCG CGTGGTCGTC GCCACCGACG ACGAACGCAT CCTGGCGGCG TGCCAGGGGT TCGGCGCCGA GGCGCTGCTG ACGCGTGCCG AGCACAACTC CGGCACCGAT CGCCTGGAGG AAGTCGCTTC CCGGCTCGGC CTGGCCAGCG ATGCCATCGT GGTCAACGTC CAGGGCGACG AACCGCTGAT CCCCCCGGCG CTCATCGACC AGGTGGCGGC CAACCTGGCG GCGCACCCGG AAGCTGCCAT CGCTACCCTG GCCGAGCCGA TCCATGAGGT CTCCGCGCTG TTCAATCCGA ACGTGGTCAA GGTCGCCACC GACATCGACG GCCTGGCCCT GACTTTCAGT CGTGCGCCCT TGCCCTGGGC TCGCGACGCC TTCGCCCGCG ATCGCGACAG CCTGCCGGAG GGCGTGCCCT ATCGTCGCCA CATCGGCATC TACGCCTATC GCGTGGGTTT CCTTGCCGAT TTCGTCGCCT GGGGACCGTG CTGGCTGGAG AACGCCGAAA GCCTTGAGCA ATTGCGCGCG CTCTGGCACG GGGTGCGAAT CCATGTGGCC GATGCCCGCG AAAACATGCT TCCGGGCGTG GATACGCCGG AAGACCTCGA ACGCGTTCGC CGCGTGTTGG GGGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT