PsaeA.01294.a
Transketolase (EC 2.2.1.1)
| CENTER ID: | PsaeA.01294.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01294.a.B1.GE38275 | full length | 1 | 665 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01294.a.B1.PW37551 | full length | 1 | 665 |
Structures
4XEU
DEPOSITED: 12/24/2014
DETERMINATION: XRay
CLONE: PsaeA.01294.a.B1.GE38275
PROTEIN: PsaeA.01294.a.B1.PW37551
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.580 |
| PGAT: | pseudomonas:PA0548 |
| RefSeq: | NP_249239.1 |
| UniProt: | Q9I5Y8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSRRERANA IRALSMDAVQ KANSGHPGAP MGMADIAEVL WRDYMQHNPS NPQWANRDRF VLSNGHGSML IYSLLHLTGY DLGIEDLKNF RQLNSRTPGH PEYGYTAGVE TTTGPLGQGI ANAVGMALAE KVLAAQFNRD GHAVVDHYTY AFLGDGCMME GISHEVASLA GTLRLNKLIA FYDDNGISID GEVHGWFTDD TPKRFEAYGW QVIRNVDGHD ADEIKTAIDT ARKSDQPTLI CCKTVIGFGS PNKQGKEECH GAPLGADEIA ATRAALGWEH APFEIPAQIY AEWDAKETGA AQEAEWNKRF AAYQAAHPEL AAELLRRLKG ELPADFAEKA AAYVADVANK GETIASRKAS QNALNAFGPL LPELLGGSAD LAGSNLTLWK GCKGVSADDA AGNYVFYGVR EFGMSAIMNG VALHGGFIPY GATFLIFMEY ARNAVRMSAL MKQRVLYVFT HDSIGLGEDG PTHQPIEQLA SLRLTPNLDT WRPADAVESA VAWKHAIERA DGPSALIFSR QNLPHQARDV AQVADIARGG YVLKDCEGEP ELILIATGSE VGLAVQAYDK LSEQGRKVRV VSMPCTSVYE QQDESYKQSV LPVEVGARIA IEAAHADYWY KYVGLDGRII GMTSFGESAP APALFEHFGF TLDNVLAVAE ELLED
NT Sequence
atgcccagcc gtcgtgagcg agccaatgcc atccgtgcac tgagcatgga tgccgtgcag aaagccaaca gcggccaccc gggcgccccg atgggcatgg ccgatatcgc cgaggtcctc tggcgcgact acatgcagca caacccgagc aacccgcagt gggccaaccg cgaccgcttc gtgctgtcca acggccacgg ctcgatgctg atctactccc tgctgcacct caccgggtac gacctcggca tcgaggacct gaagaacttc cgccagctca actcgcgcac cccgggccac ccggagtacg gctacaccgc cggcgtcgag accaccaccg gtccgctcgg ccagggcatc gccaatgcgg tgggcatggc gctggcggag aaggtcctgg ccgcccagtt caaccgcgac ggccacgcgg tggtcgacca ctacacctac gccttcctcg gcgacggctg catgatggaa ggcatttccc atgaggtcgc ctcgctggcc ggcaccctgc gcctgaacaa gctgatcgcc ttctacgacg acaacggcat ttccatcgac ggcgaggtcc acggctggtt caccgacgac accccgaagc gcttcgaggc ctatggctgg caagtgatcc gcaacgtcga cgggcatgac gccgacgaga tcaagaccgc catcgatacc gcgcgcaaga gcgaccagcc gaccctgatc tgctgcaaga ccgtgatcgg tttcggctcg ccgaacaagc agggcaagga agagtgccac ggcgcgccgc tgggcgccga cgagatcgcc gcgacccgcg ccgcgctggg ctgggagcac gctccgttcg agatcccggc gcagatctac gccgagtggg acgccaagga aaccggcgcc gcccaggaag ccgagtggaa caagcgtttc gccgcctacc aggctgccca tccggaactg gccgccgaat tgctgcgccg cctgaagggc gagctgccgg ccgacttcgc cgagaaggcc gcggcctacg tcgccgatgt tgccaacaag ggtgagacca tcgccagccg caaggccagc cagaacgcgc tgaacgcctt cggcccgctg ctgccggagc tgctcggcgg ttccgccgac ctggccggct ccaacctgac cttgtggaag ggctgcaagg gcgtcagcgc cgacgacgcc gccggcaact acgtgttcta cggcgtgcgc gaattcggca tgagcgcgat catgaatggc gtcgccctgc acggcggttt cattccctac ggtgcgacct tcctgatctt catggaatac gcgcgcaacg ccgtgcgcat gtccgcactg atgaagcagc gcgtgctcta cgtgttcacc cacgactcca tcggcctcgg cgaggacggc ccgacccacc agccgatcga acaactggcc agcctgcgcc tgaccccgaa cctggacacc tggcgcccgg ccgacgcggt cgagtcggcg gtggcctgga agcatgccat cgagcgcgcc gacggtccgt ccgcgctgat cttctcccgc cagaacctgc cgcaccaggc gcgcgacgtc gcccaggtgg ccgacatcgc ccgcggcggc tacgtgctga aggactgcga aggcgagccg gaactgatcc tgatcgccac cggttcggaa gtcggcctgg ccgtgcaggc ctacgacaag ctcagcgagc agggccgcaa ggtccgcgtg gtatcgatgc catgcaccag cgtctacgag cagcaggacg agtcctacaa gcagtccgtg ctgccggtgg aagtcggcgc gcgcatcgcc atcgaggccg cccatgccga ctactggtac aagtacgtcg gtctcgacgg gcgcatcatc ggcatgacca gcttcggcga gtcggcgccg gccccggcgc tgttcgagca cttcggcttc accctggaca acgtcctggc ggtggccgag gagctgctgg aagactga
Details for PsaeA.01294.a.B1.GE38275
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 73kDa |
| OBSERVED MW: | 79kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 83 |
| PERCENT COVERAGE: | 57 |
Validated AA Sequence
MAHHHHHHMP SRRERANAIR ALSMDAVQKA NSGHPGAPMG MADIAEVLWR DYMQHNPSNP QWANRDRFVL SNGHGSMLIY SLLHLTGYDL GIEDLKNFRQ LNSRTPGHPE YGYTAGVETT TGPLGQGIAN AVGMALAEKV LAAQFNRDGH AVVDHYTYAF LGDGCMMEGI SHEVASLAGT LRLNKLIAFY DDNGISIDGE VHGWFTDDTP KRFEAYGWQV IRNVDGHDAD EIKTAIDTAR KSDQPTLICC KTVIGFGSPN KQGKEECHGA PLGADEIAAT RAALGWEHAP FEIPAQIYAE WDAKETGAAQ EAEXTSVSPP TRLPIRNWRD AAPPEXELXX FXXXRXRXXX XRVRHXXXXX XXAXXXXLXT XXXXXXXXXX XTVXGXXG
Validated NT Sequence
cnnnnnnnnn nnnntngant aattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatgcc cagccgtcgt gagcgagcca atgccatccg tgcactgagc atggatgccg tgcagaaagc caacagcggc cacccgggcg ccccgatggg catggccgat atcgccgagg tcctctggcg cgactacatg cagcacaacc cgagcaaccc gcagtgggcc aaccgcgacc gcttcgtgct gtccaacggc cacggctcga tgctgatcta ctccctgctg cacctcaccg ggtacgacct cggcatcgag gacctgaaga acttccgcca gctcaactcg cgcaccccgg gccacccgga gtacggctac accgccggcg tcgagaccac caccggtccg ctcggccagg gcatcgccaa tgcggtgggc atggcgctgg cggagaaggt cctggccgcc cagttcaacc gcgacggcca cgcggtggtc gaccactaca cctacgcctt cctcggcgac ggctgcatga tggaaggcat ttcccatgag gtcgcctcgc tggccggcac cctgcgcctg aacaagctga tcgccttcta cgacgacaac ggcatttcca tcgacggcga ggtccacggc tggttcaccg acgacacccc gaagcgcttc gaggcctatg gctggcaagt gatccgcaac gtcgacgggc atgacgccga cgagatcaag accgccatcg ataccgcgcg caagagcgac cagccgaccc tgatctgctg caagaccgtg atcggtttcg gctcgccgaa caagcagggc aaggaagagt gccacggcgc gccgctgggc gccgacgaga tcgccgcgac ccgcgccgcg ctgggctggg agcacgctcc gttcgagatc ccggcgcaga tctacgccga gtgggacgcc aaggaaaccg gcgccgccca ggaagccgag tnnacnagcg tttcgccgcc taccaggctg cccatccgga actggcgcga tgctgcgccg cctgaanncg agctgnnnna cttcncnann nnncggnnac gtcncnatnt gcnnagggtg agacatcncn gcngcagnnn cnnangngct nannccnnnt nnctncnnac tngnnnnnnn ncgcnnnncn gnnnnnnnnc acnnactgtn nnnggnnnnc anggggg
Expected Protein Sequence
MAHHHHHHMP SRRERANAIR ALSMDAVQKA NSGHPGAPMG MADIAEVLWR DYMQHNPSNP QWANRDRFVL SNGHGSMLIY SLLHLTGYDL GIEDLKNFRQ LNSRTPGHPE YGYTAGVETT TGPLGQGIAN AVGMALAEKV LAAQFNRDGH AVVDHYTYAF LGDGCMMEGI SHEVASLAGT LRLNKLIAFY DDNGISIDGE VHGWFTDDTP KRFEAYGWQV IRNVDGHDAD EIKTAIDTAR KSDQPTLICC KTVIGFGSPN KQGKEECHGA PLGADEIAAT RAALGWEHAP FEIPAQIYAE WDAKETGAAQ EAEWNKRFAA YQAAHPELAA ELLRRLKGEL PADFAEKAAA YVADVANKGE TIASRKASQN ALNAFGPLLP ELLGGSADLA GSNLTLWKGC KGVSADDAAG NYVFYGVREF GMSAIMNGVA LHGGFIPYGA TFLIFMEYAR NAVRMSALMK QRVLYVFTHD SIGLGEDGPT HQPIEQLASL RLTPNLDTWR PADAVESAVA WKHAIERADG PSALIFSRQN LPHQARDVAQ VADIARGGYV LKDCEGEPEL ILIATGSEVG LAVQAYDKLS EQGRKVRVVS MPCTSVYEQQ DESYKQSVLP VEVGARIAIE AAHADYWYKY VGLDGRIIGM TSFGESAPAP ALFEHFGFTL DNVLAVAEEL LED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca gccgtcgtga gcgagccaat gccatccgtg cactgagcat ggatgccgtg cagaaagcca acagcggcca cccgggcgcc ccgatgggca tggccgatat cgccgaggtc ctctggcgcg actacatgca gcacaacccg agcaacccgc agtgggccaa ccgcgaccgc ttcgtgctgt ccaacggcca cggctcgatg ctgatctact ccctgctgca cctcaccggg tacgacctcg gcatcgagga cctgaagaac ttccgccagc tcaactcgcg caccccgggc cacccggagt acggctacac cgccggcgtc gagaccacca ccggtccgct cggccagggc atcgccaatg cggtgggcat ggcgctggcg gagaaggtcc tggccgccca gttcaaccgc gacggccacg cggtggtcga ccactacacc tacgccttcc tcggcgacgg ctgcatgatg gaaggcattt cccatgaggt cgcctcgctg gccggcaccc tgcgcctgaa caagctgatc gccttctacg acgacaacgg catttccatc gacggcgagg tccacggctg gttcaccgac gacaccccga agcgcttcga ggcctatggc tggcaagtga tccgcaacgt cgacgggcat gacgccgacg agatcaagac cgccatcgat accgcgcgca agagcgacca gccgaccctg atctgctgca agaccgtgat cggtttcggc tcgccgaaca agcagggcaa ggaagagtgc cacggcgcgc cgctgggcgc cgacgagatc gccgcgaccc gcgccgcgct gggctgggag cacgctccgt tcgagatccc ggcgcagatc tacgccgagt gggacgccaa ggaaaccggc gccgcccagg aagccgagtg gaacaagcgt ttcgccgcct accaggctgc ccatccggaa ctggccgccg aattgctgcg ccgcctgaag ggcgagctgc cggccgactt cgccgagaag gccgcggcct acgtcgccga tgttgccaac aagggtgaga ccatcgccag ccgcaaggcc agccagaacg cgctgaacgc cttcggcccg ctgctgccgg agctgctcgg cggttccgcc gacctggccg gctccaacct gaccttgtgg aagggctgca agggcgtcag cgccgacgac gccgccggca actacgtgtt ctacggcgtg cgcgaattcg gcatgagcgc gatcatgaat ggcgtcgccc tgcacggcgg tttcattccc tacggtgcga ccttcctgat cttcatggaa tacgcgcgca acgccgtgcg catgtccgca ctgatgaagc agcgcgtgct ctacgtgttc acccacgact ccatcggcct cggcgaggac ggcccgaccc accagccgat cgaacaactg gccagcctgc gcctgacccc gaacctggac acctggcgcc cggccgacgc ggtcgagtcg gcggtggcct ggaagcatgc catcgagcgc gccgacggtc cgtccgcgct gatcttctcc cgccagaacc tgccgcacca ggcgcgcgac gtcgcccagg tggccgacat cgcccgcggc ggctacgtgc tgaaggactg cgaaggcgag ccggaactga tcctgatcgc caccggttcg gaagtcggcc tggccgtgca ggcctacgac aagctcagcg agcagggccg caaggtccgc gtggtatcga tgccatgcac cagcgtctac gagcagcagg acgagtccta caagcagtcc gtgctgccgg tggaagtcgg cgcgcgcatc gccatcgagg ccgcccatgc cgactactgg tacaagtacg tcggtctcga cgggcgcatc atcggcatga ccagcttcgg cgagtcggcg ccggccccgg cgctgttcga gcacttcggc ttcaccctgg acaacgtcct ggcggtggcc gaggagctgc tggaagactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.01294.a.B1.PW37551
| PURIFICATION DATe: | 9/8/2014 |
| CONCENTRATION: | 39mg/ml |
| OBSERVED MW: | 75kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 83 |
| PERCENT COVERAGE: | 57 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP SRRERANAIR ALSMDAVQKA NSGHPGAPMG MADIAEVLWR DYMQHNPSNP QWANRDRFVL SNGHGSMLIY SLLHLTGYDL GIEDLKNFRQ LNSRTPGHPE YGYTAGVETT TGPLGQGIAN AVGMALAEKV LAAQFNRDGH AVVDHYTYAF LGDGCMMEGI SHEVASLAGT LRLNKLIAFY DDNGISIDGE VHGWFTDDTP KRFEAYGWQV IRNVDGHDAD EIKTAIDTAR KSDQPTLICC KTVIGFGSPN KQGKEECHGA PLGADEIAAT RAALGWEHAP FEIPAQIYAE WDAKETGAAQ EAEXTSVSPP TRLPIRNWRD AAPPEXELXX FXXXRXRXXX XRVRHXXXXX XXAXXXXLXT XXXXXXXXXX XTVXGXXG
Validated NT Sequence
cnnnnnnnnn nnnntngant aattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatgcc cagccgtcgt gagcgagcca atgccatccg tgcactgagc atggatgccg tgcagaaagc caacagcggc cacccgggcg ccccgatggg catggccgat atcgccgagg tcctctggcg cgactacatg cagcacaacc cgagcaaccc gcagtgggcc aaccgcgacc gcttcgtgct gtccaacggc cacggctcga tgctgatcta ctccctgctg cacctcaccg ggtacgacct cggcatcgag gacctgaaga acttccgcca gctcaactcg cgcaccccgg gccacccgga gtacggctac accgccggcg tcgagaccac caccggtccg ctcggccagg gcatcgccaa tgcggtgggc atggcgctgg cggagaaggt cctggccgcc cagttcaacc gcgacggcca cgcggtggtc gaccactaca cctacgcctt cctcggcgac ggctgcatga tggaaggcat ttcccatgag gtcgcctcgc tggccggcac cctgcgcctg aacaagctga tcgccttcta cgacgacaac ggcatttcca tcgacggcga ggtccacggc tggttcaccg acgacacccc gaagcgcttc gaggcctatg gctggcaagt gatccgcaac gtcgacgggc atgacgccga cgagatcaag accgccatcg ataccgcgcg caagagcgac cagccgaccc tgatctgctg caagaccgtg atcggtttcg gctcgccgaa caagcagggc aaggaagagt gccacggcgc gccgctgggc gccgacgaga tcgccgcgac ccgcgccgcg ctgggctggg agcacgctcc gttcgagatc ccggcgcaga tctacgccga gtgggacgcc aaggaaaccg gcgccgccca ggaagccgag tnnacnagcg tttcgccgcc taccaggctg cccatccgga actggcgcga tgctgcgccg cctgaanncg agctgnnnna cttcncnann nnncggnnac gtcncnatnt gcnnagggtg agacatcncn gcngcagnnn cnnangngct nannccnnnt nnctncnnac tngnnnnnnn ncgcnnnncn gnnnnnnnnc acnnactgtn nnnggnnnnc anggggg
Expressed Protein Sequence
MAHHHHHHMP SRRERANAIR ALSMDAVQKA NSGHPGAPMG MADIAEVLWR DYMQHNPSNP QWANRDRFVL SNGHGSMLIY SLLHLTGYDL GIEDLKNFRQ LNSRTPGHPE YGYTAGVETT TGPLGQGIAN AVGMALAEKV LAAQFNRDGH AVVDHYTYAF LGDGCMMEGI SHEVASLAGT LRLNKLIAFY DDNGISIDGE VHGWFTDDTP KRFEAYGWQV IRNVDGHDAD EIKTAIDTAR KSDQPTLICC KTVIGFGSPN KQGKEECHGA PLGADEIAAT RAALGWEHAP FEIPAQIYAE WDAKETGAAQ EAEWNKRFAA YQAAHPELAA ELLRRLKGEL PADFAEKAAA YVADVANKGE TIASRKASQN ALNAFGPLLP ELLGGSADLA GSNLTLWKGC KGVSADDAAG NYVFYGVREF GMSAIMNGVA LHGGFIPYGA TFLIFMEYAR NAVRMSALMK QRVLYVFTHD SIGLGEDGPT HQPIEQLASL RLTPNLDTWR PADAVESAVA WKHAIERADG PSALIFSRQN LPHQARDVAQ VADIARGGYV LKDCEGEPEL ILIATGSEVG LAVQAYDKLS EQGRKVRVVS MPCTSVYEQQ DESYKQSVLP VEVGARIAIE AAHADYWYKY VGLDGRIIGM TSFGESAPAP ALFEHFGFTL DNVLAVAEEL LED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCA GCCGTCGTGA GCGAGCCAAT GCCATCCGTG CACTGAGCAT GGATGCCGTG CAGAAAGCCA ACAGCGGCCA CCCGGGCGCC CCGATGGGCA TGGCCGATAT CGCCGAGGTC CTCTGGCGCG ACTACATGCA GCACAACCCG AGCAACCCGC AGTGGGCCAA CCGCGACCGC TTCGTGCTGT CCAACGGCCA CGGCTCGATG CTGATCTACT CCCTGCTGCA CCTCACCGGG TACGACCTCG GCATCGAGGA CCTGAAGAAC TTCCGCCAGC TCAACTCGCG CACCCCGGGC CACCCGGAGT ACGGCTACAC CGCCGGCGTC GAGACCACCA CCGGTCCGCT CGGCCAGGGC ATCGCCAATG CGGTGGGCAT GGCGCTGGCG GAGAAGGTCC TGGCCGCCCA GTTCAACCGC GACGGCCACG CGGTGGTCGA CCACTACACC TACGCCTTCC TCGGCGACGG CTGCATGATG GAAGGCATTT CCCATGAGGT CGCCTCGCTG GCCGGCACCC TGCGCCTGAA CAAGCTGATC GCCTTCTACG ACGACAACGG CATTTCCATC GACGGCGAGG TCCACGGCTG GTTCACCGAC GACACCCCGA AGCGCTTCGA GGCCTATGGC TGGCAAGTGA TCCGCAACGT CGACGGGCAT GACGCCGACG AGATCAAGAC CGCCATCGAT ACCGCGCGCA AGAGCGACCA GCCGACCCTG ATCTGCTGCA AGACCGTGAT CGGTTTCGGC TCGCCGAACA AGCAGGGCAA GGAAGAGTGC CACGGCGCGC CGCTGGGCGC CGACGAGATC GCCGCGACCC GCGCCGCGCT GGGCTGGGAG CACGCTCCGT TCGAGATCCC GGCGCAGATC TACGCCGAGT GGGACGCCAA GGAAACCGGC GCCGCCCAGG AAGCCGAGTG GAACAAGCGT TTCGCCGCCT ACCAGGCTGC CCATCCGGAA CTGGCCGCCG AATTGCTGCG CCGCCTGAAG GGCGAGCTGC CGGCCGACTT CGCCGAGAAG GCCGCGGCCT ACGTCGCCGA TGTTGCCAAC AAGGGTGAGA CCATCGCCAG CCGCAAGGCC AGCCAGAACG CGCTGAACGC CTTCGGCCCG CTGCTGCCGG AGCTGCTCGG CGGTTCCGCC GACCTGGCCG GCTCCAACCT GACCTTGTGG AAGGGCTGCA AGGGCGTCAG CGCCGACGAC GCCGCCGGCA ACTACGTGTT CTACGGCGTG CGCGAATTCG GCATGAGCGC GATCATGAAT GGCGTCGCCC TGCACGGCGG TTTCATTCCC TACGGTGCGA CCTTCCTGAT CTTCATGGAA TACGCGCGCA ACGCCGTGCG CATGTCCGCA CTGATGAAGC AGCGCGTGCT CTACGTGTTC ACCCACGACT CCATCGGCCT CGGCGAGGAC GGCCCGACCC ACCAGCCGAT CGAACAACTG GCCAGCCTGC GCCTGACCCC GAACCTGGAC ACCTGGCGCC CGGCCGACGC GGTCGAGTCG GCGGTGGCCT GGAAGCATGC CATCGAGCGC GCCGACGGTC CGTCCGCGCT GATCTTCTCC CGCCAGAACC TGCCGCACCA GGCGCGCGAC GTCGCCCAGG TGGCCGACAT CGCCCGCGGC GGCTACGTGC TGAAGGACTG CGAAGGCGAG CCGGAACTGA TCCTGATCGC CACCGGTTCG GAAGTCGGCC TGGCCGTGCA GGCCTACGAC AAGCTCAGCG AGCAGGGCCG CAAGGTCCGC GTGGTATCGA TGCCATGCAC CAGCGTCTAC GAGCAGCAGG ACGAGTCCTA CAAGCAGTCC GTGCTGCCGG TGGAAGTCGG CGCGCGCATC GCCATCGAGG CCGCCCATGC CGACTACTGG TACAAGTACG TCGGTCTCGA CGGGCGCATC ATCGGCATGA CCAGCTTCGG CGAGTCGGCG CCGGCCCCGG CGCTGTTCGA GCACTTCGGC TTCACCCTGG ACAACGTCCT GGCGGTGGCC GAGGAGCTGC TGGAAGACTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT