PsaeA.01250.a

Probable inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase) (EC 2.7.1.23)

CENTER ID: PsaeA.01250.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.01250.a.B1.GE38274 full length 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.01250.a.B1.PW37561 full length 1 295

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3240
PGAT: pseudomonas:PA3088
RefSeq: NP_251778.1
UniProt: Q9HZC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPFRNIGII GRLGSTQVLD TIRRLKKFLI DRHLHVILED TIAEVLPGHG LQTCSRKIMG EICDLVVVVG GDGSMLGAAR ALARHKVPVL GINRGSLGFL TDIRPDELEA KVGEVLDGQY IVESRFLLDA QVRRGIDSMG QGDALNDVVL HPGKSTRMIE FELYIDGQFV CSQKADGLIV ATPTGSTAYA LSAGGPIMHP KLDAIVIVPM YPHMLSSRPI VVDGNSELKI VVSPNMQIYP QVSCDGQNHF TCAPGDTVTI SKKPQKLRLI HPIDHNYYEI CRTKLGWGSR LGGGD
NT Sequence
atggaaccct ttcgcaatat cggcatcatc ggccgcctcg gcagcaccca ggtgctggac accattcgcc ggctgaagaa attcctgatc gatcgccacc tgcatgtgat cctcgaggac accatcgccg aagtcctgcc cggacacggc ctgcagacct gctcgcggaa gatcatgggc gagatctgcg acctggtggt ggtggtcggc ggcgatggca gcatgctcgg cgcggcccgc gcgctggccc ggcacaaggt gccggtgctg gggatcaacc gcggcagcct gggcttcctc accgacatcc gcccggatga actggaggcc aaggtcggcg aggtgctgga cggccagtac atcgtcgaga gccgcttcct cctcgatgcc caggtgcgtc gcggtatcga ctcgatgggg cagggcgatg cgctcaacga cgtggtcctg cacccgggca agtcgacccg gatgatcgag ttcgagctgt atatcgacgg ccagttcgtc tgcagccaga aggccgacgg cctgatcgtc gccacgccca ccggttccac cgcctacgcg ctgtccgccg gcggtccgat catgcatccg aagctggatg ccatcgtcat cgtgccgatg tacccgcaca tgctttccag ccgcccgatc gtggtggacg gcaacagcga gctgaagatc gtcgtgtcgc cgaacatgca gatctacccg caggtctcct gcgacgggca gaaccatttc acctgcgccc ccggcgatac cgtgacgatc agcaagaagc cgcagaagct gcgcctgatc caccccatcg accacaacta ctacgagatc tgccggacca agctcggctg gggcagccgc ctgggcggag gcgactga
Details for PsaeA.01250.a.B1.GE38274
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME PFRNIGIIGR LGSTQVLDTI RRLKKFLIDR HLHVILEDTI AEVLPGHGLQ TCSRKIMGEI CDLVVVVGGD GSMLGAARAL ARHKVPVLGI NRGSLGFLTD IRPDELEAKV GEVLDGQYIV ESRFLLDAQV RRGIDSMGQG DALNDVVLHP GKSTRMIEFE LYIDGQFVCS QKADGLIVAT PTGSTAYALS AGGPIMHPKL DAIVIVPMYP HMLSSRPIVV DGNSELKIVV SPNMQIYPQV SCDGQNHFTC APGDTVTISK KPQKLRLIHP IDHNYYEICR TKLGWGSRLG GGD
Validated NT Sequence
atcttactca cttagtcgcc tccgcccagg cggctgcccc agccgagctt ggtccggcag atctcgtagt agttgtggtc gatggggtgg atcaggcgca gcttctgcgg cttcttgctg atcgtcacgg tatcgccggg ggcgcaggtg aaatggttct gcccgtcgca ggagacctgc gggtagatct gcatgttcgg cgacacgacg atcttcagct cgctgttgcc gtccaccacg atcgggcggc tggaaagcat gtgcgggtac atcggcacga tgacgatggc atccagcttc ggatgcatga tcggaccgcc ggcggacagc gcgtaggcgg tggaaccggt gggcgtggcg acgatcaggc cgtcggcctt ctggctgcag acgaactggc cgtcgatata cagctcgaac tcgatcatcc gggtcgactt gcccgggtgc aggaccacgt cgttgagcgc atcgccctgc cccatcgagt cgataccgcg acgcacctgg gcatcgagga ggaagcggct ctcgacgatg tactggccgt ccagcacctc gccgaccttg gcctccagtt catccgggcg gatgtcggtg aggaagccca ggctgccgcg gttgatcccc agcaccggca ccttgtgccg ggccagcgcg cgggccgcgc cgagcatgct gccatcgccg ccgaccacca ccaccaggtc gcagatctcg cccatgatct tccgcgagca ggtctgcagg ccgtgtccgg gcaggacttc ggcgatggtg tcctcgagga tcacatgcag gtggcgatcg atcaggaatt tcttcagccg gcgaatggtg tccagcacct gggtgctgcc gaggcggccg atgatgccga tattgcgaaa gggttccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHME PFRNIGIIGR LGSTQVLDTI RRLKKFLIDR HLHVILEDTI AEVLPGHGLQ TCSRKIMGEI CDLVVVVGGD GSMLGAARAL ARHKVPVLGI NRGSLGFLTD IRPDELEAKV GEVLDGQYIV ESRFLLDAQV RRGIDSMGQG DALNDVVLHP GKSTRMIEFE LYIDGQFVCS QKADGLIVAT PTGSTAYALS AGGPIMHPKL DAIVIVPMYP HMLSSRPIVV DGNSELKIVV SPNMQIYPQV SCDGQNHFTC APGDTVTISK KPQKLRLIHP IDHNYYEICR TKLGWGSRLG GGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac cctttcgcaa tatcggcatc atcggccgcc tcggcagcac ccaggtgctg gacaccattc gccggctgaa gaaattcctg atcgatcgcc acctgcatgt gatcctcgag gacaccatcg ccgaagtcct gcccggacac ggcctgcaga cctgctcgcg gaagatcatg ggcgagatct gcgacctggt ggtggtggtc ggcggcgatg gcagcatgct cggcgcggcc cgcgcgctgg cccggcacaa ggtgccggtg ctggggatca accgcggcag cctgggcttc ctcaccgaca tccgcccgga tgaactggag gccaaggtcg gcgaggtgct ggacggccag tacatcgtcg agagccgctt cctcctcgat gcccaggtgc gtcgcggtat cgactcgatg gggcagggcg atgcgctcaa cgacgtggtc ctgcacccgg gcaagtcgac ccggatgatc gagttcgagc tgtatatcga cggccagttc gtctgcagcc agaaggccga cggcctgatc gtcgccacgc ccaccggttc caccgcctac gcgctgtccg ccggcggtcc gatcatgcat ccgaagctgg atgccatcgt catcgtgccg atgtacccgc acatgctttc cagccgcccg atcgtggtgg acggcaacag cgagctgaag atcgtcgtgt cgccgaacat gcagatctac ccgcaggtct cctgcgacgg gcagaaccat ttcacctgcg cccccggcga taccgtgacg atcagcaaga agccgcagaa gctgcgcctg atccacccca tcgaccacaa ctactacgag atctgccgga ccaagctcgg ctggggcagc cgcctgggcg gaggcgactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.01250.a.B1.PW37561
PURIFICATION DATe: 9/16/2014
CONCENTRATION: 8mg/ml
OBSERVED MW: 31kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME PFRNIGIIGR LGSTQVLDTI RRLKKFLIDR HLHVILEDTI AEVLPGHGLQ TCSRKIMGEI CDLVVVVGGD GSMLGAARAL ARHKVPVLGI NRGSLGFLTD IRPDELEAKV GEVLDGQYIV ESRFLLDAQV RRGIDSMGQG DALNDVVLHP GKSTRMIEFE LYIDGQFVCS QKADGLIVAT PTGSTAYALS AGGPIMHPKL DAIVIVPMYP HMLSSRPIVV DGNSELKIVV SPNMQIYPQV SCDGQNHFTC APGDTVTISK KPQKLRLIHP IDHNYYEICR TKLGWGSRLG GGD
Validated NT Sequence
atcttactca cttagtcgcc tccgcccagg cggctgcccc agccgagctt ggtccggcag atctcgtagt agttgtggtc gatggggtgg atcaggcgca gcttctgcgg cttcttgctg atcgtcacgg tatcgccggg ggcgcaggtg aaatggttct gcccgtcgca ggagacctgc gggtagatct gcatgttcgg cgacacgacg atcttcagct cgctgttgcc gtccaccacg atcgggcggc tggaaagcat gtgcgggtac atcggcacga tgacgatggc atccagcttc ggatgcatga tcggaccgcc ggcggacagc gcgtaggcgg tggaaccggt gggcgtggcg acgatcaggc cgtcggcctt ctggctgcag acgaactggc cgtcgatata cagctcgaac tcgatcatcc gggtcgactt gcccgggtgc aggaccacgt cgttgagcgc atcgccctgc cccatcgagt cgataccgcg acgcacctgg gcatcgagga ggaagcggct ctcgacgatg tactggccgt ccagcacctc gccgaccttg gcctccagtt catccgggcg gatgtcggtg aggaagccca ggctgccgcg gttgatcccc agcaccggca ccttgtgccg ggccagcgcg cgggccgcgc cgagcatgct gccatcgccg ccgaccacca ccaccaggtc gcagatctcg cccatgatct tccgcgagca ggtctgcagg ccgtgtccgg gcaggacttc ggcgatggtg tcctcgagga tcacatgcag gtggcgatcg atcaggaatt tcttcagccg gcgaatggtg tccagcacct gggtgctgcc gaggcggccg atgatgccga tattgcgaaa gggttccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHME PFRNIGIIGR LGSTQVLDTI RRLKKFLIDR HLHVILEDTI AEVLPGHGLQ TCSRKIMGEI CDLVVVVGGD GSMLGAARAL ARHKVPVLGI NRGSLGFLTD IRPDELEAKV GEVLDGQYIV ESRFLLDAQV RRGIDSMGQG DALNDVVLHP GKSTRMIEFE LYIDGQFVCS QKADGLIVAT PTGSTAYALS AGGPIMHPKL DAIVIVPMYP HMLSSRPIVV DGNSELKIVV SPNMQIYPQV SCDGQNHFTC APGDTVTISK KPQKLRLIHP IDHNYYEICR TKLGWGSRLG GGD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAC CCTTTCGCAA TATCGGCATC ATCGGCCGCC TCGGCAGCAC CCAGGTGCTG GACACCATTC GCCGGCTGAA GAAATTCCTG ATCGATCGCC ACCTGCATGT GATCCTCGAG GACACCATCG CCGAAGTCCT GCCCGGACAC GGCCTGCAGA CCTGCTCGCG GAAGATCATG GGCGAGATCT GCGACCTGGT GGTGGTGGTC GGCGGCGATG GCAGCATGCT CGGCGCGGCC CGCGCGCTGG CCCGGCACAA GGTGCCGGTG CTGGGGATCA ACCGCGGCAG CCTGGGCTTC CTCACCGACA TCCGCCCGGA TGAACTGGAG GCCAAGGTCG GCGAGGTGCT GGACGGCCAG TACATCGTCG AGAGCCGCTT CCTCCTCGAT GCCCAGGTGC GTCGCGGTAT CGACTCGATG GGGCAGGGCG ATGCGCTCAA CGACGTGGTC CTGCACCCGG GCAAGTCGAC CCGGATGATC GAGTTCGAGC TGTATATCGA CGGCCAGTTC GTCTGCAGCC AGAAGGCCGA CGGCCTGATC GTCGCCACGC CCACCGGTTC CACCGCCTAC GCGCTGTCCG CCGGCGGTCC GATCATGCAT CCGAAGCTGG ATGCCATCGT CATCGTGCCG ATGTACCCGC ACATGCTTTC CAGCCGCCCG ATCGTGGTGG ACGGCAACAG CGAGCTGAAG ATCGTCGTGT CGCCGAACAT GCAGATCTAC CCGCAGGTCT CCTGCGACGG GCAGAACCAT TTCACCTGCG CCCCCGGCGA TACCGTGACG ATCAGCAAGA AGCCGCAGAA GCTGCGCCTG ATCCACCCCA TCGACCACAA CTACTACGAG ATCTGCCGGA CCAAGCTCGG CTGGGGCAGC CGCCTGGGCG GAGGCGACTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT