PsaeA.01152.a
Uroporphyrinogen decarboxylase (UPD) (URO-D) (EC 4.1.1.37)
| CENTER ID: | PsaeA.01152.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01152.a.B1.GE38273 | full length | 1 | 355 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01152.a.B1.PS02161 | full length | 1 | 355 |
Structures
4WSH
DEPOSITED: 10/27/2014
DETERMINATION: XRay
CLONE: PsaeA.01152.a.B1.GE38273
PROTEIN: PsaeA.01152.a.B1.PS02161
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.5277 |
| PGAT: | pseudomonas:PA5034 |
| RefSeq: | NP_253721.1 |
| UniProt: | P95458 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTALKNDRFL RALLKQPVDV TPVWMMRQAG RYLPEYRATR AKAGDFMSLC MNPELACEVT LQPLDRYPQL DAAILFSDIL TIPDAMGQGL YFETGEGPRF RKVVSSLADI EALPVPDPEQ DLGYVMDAVR TIRRELNGRV PLIGFSGSPW TLATYMVEGG SSKDFRKSKA MLYDNPKAMH ALLDKLAQSV TSYLNGQIHA GAQAVQIFDS WGGSLSAAAY QEFSLAYMRK IVDGLIREHD GRRVPVILFT KGGGLWLESM AEVGAEALGL DWTCDIGSAR ARVGERVALQ GNMDPSVLYA NPAAIRAEVA RILAAYGKGT GHVFNLGHGI TPEVDPAHAG AFFEAVHELS AQYHG
NT Sequence
ATGACTGCCC TGAAGAACGA CCGCTTCCTC CGCGCCCTGC TCAAGCAGCC CGTCGATGTC ACCCCCGTCT GGATGATGCG CCAGGCCGGT CGTTACCTGC CGGAATACCG TGCCACCCGT GCCAAGGCCG GCGATTTCAT GAGCCTGTGC ATGAACCCGG AGCTGGCCTG CGAAGTGACC CTGCAGCCGC TGGACCGTTA CCCGCAGCTG GACGCCGCGA TCCTCTTCTC CGATATCCTG ACCATTCCCG ATGCCATGGG CCAGGGCCTG TACTTCGAGA CCGGCGAAGG CCCGCGCTTC CGCAAGGTGG TCTCGTCGCT GGCCGACATC GAGGCGCTGC CGGTTCCCGA TCCGGAACAG GACCTGGGCT ATGTGATGGA CGCCGTGCGC ACCATCCGCC GCGAGCTGAA CGGCCGCGTA CCGCTGATCG GCTTCTCCGG CAGCCCCTGG ACCCTCGCCA CCTACATGGT CGAGGGCGGC TCGAGCAAGG ACTTCCGCAA GTCCAAGGCG ATGCTCTACG ACAACCCCAA GGCCATGCAC GCTCTGCTGG ACAAGCTGGC GCAATCGGTG ACCAGCTACC TCAACGGGCA GATCCACGCC GGCGCCCAGG CGGTGCAGAT CTTCGACTCA TGGGGCGGCA GCCTGTCGGC GGCGGCCTAC CAGGAGTTCT CCCTGGCCTA CATGCGCAAG ATCGTCGATG GATTGATCCG CGAGCACGAC GGACGCCGGG TGCCGGTGAT CCTCTTCACC AAGGGAGGCG GCCTGTGGCT GGAGTCGATG GCCGAGGTCG GCGCCGAGGC GCTGGGCCTG GACTGGACCT GCGACATCGG CAGCGCCCGG GCCCGTGTCG GTGAGCGCGT AGCCCTGCAA GGCAACATGG ATCCTTCGGT GCTCTACGCC AATCCGGCGG CGATCCGCGC CGAAGTCGCG CGGATCCTGG CGGCCTACGG CAAGGGTACG GGGCATGTGT TCAATCTCGG CCACGGCATC ACCCCGGAAG TCGACCCGGC CCATGCCGGC GCCTTCTTCG AGGCGGTGCA CGAGCTGTCG GCGCAGTACC ACGGCTGA
Details for PsaeA.01152.a.B1.GE38273
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MTALKNDRFL RALLKQPVDV TPVWMMRQAG RYLPEYRATR AKAGDFMSLC MNPELACEVT LQPLDRYPQL DAAILFSDIL TIPDAMGQGL YFETGEGPRF RKVVSSLADI EALPVPDPEQ DLGYVMDAVR TIRRELNGRV PLIGFSGSPW TLATYMVEGG SSKDFRKSKA MLYDNPKAMH ALLDKLAQSV TSYLNGQIHA GAQAVQIFDS WGGSLSAAAY QEFSLAYMRK IVDGLIREHD GRRVPVILFT KGGGLWLESM AEVGAEALGL DWTCDIGSAR ARVGERVALQ GNMDPSVLYA NPAAIRAEVA RILAAYGKGT GHVFNLGHGI TPEVDPAHAG AFFEAVHELS AQYH
Validated NT Sequence
cgtggtactg cgccgacagc tcgtgcaccg cctcgaagaa ggcgccggca tgggccgggt cgacttccgg ggtgatgccg tggccgagat tgaacacatg ccccgtaccc ttgccgtagg ccgccaggat ccgcgcgact tcggcgcgga tcgccgccgg attggcgtag agcaccgaag gatccatgtt gccttgcagg gctacgcgct caccgacacg ggcccgggcg ctgccgatgt cgcaggtcca gtccaggccc agcgcctcgg cgccgacctc ggccatcgac tccagccaca ggccgcctcc cttggtgaag aggatcaccg gcacccggcg tccgtcgtgc tcgcggatca atccatcgac gatcttgcgc atgtaggcca gggagaactc ctggtaggcc gccgccgaca ggctgccgcc ccatgagtcg aagatctgca ccgcctgggc gccggcgtgg atctgcccgt tgaggtagct ggtcaccgat tgcgccagct tgtccagcag agcgtgcatg gccttggggt tgtcgtagag catcgccttg gacttgcgga agtccttgct cgagccgccc tcgaccatgt aggtggcgag ggtccagggg ctgccggaga agccgatcag cggtacgcgg ccgttcagct cgcggcggat ggtgcgcacg gcgtccatca catagcccag gtcctgttcc ggatcgggaa ccggcagcgc ctcgatgtcg gccagcgacg agaccacctt gcggaagcgc gggccttcgc cggtctcgaa gtacaggccc tggcccatgg catcgggaat ggtcaggata tcggagaaga ggatcgcggc gtccagctgc gggtaacggt ccagcggctg cagggtcact tcgcaggcca gctccgggtt catgcacagg ctcatgaaat cgccggcctt ggcacgggtg gcacggtatt ccggcaggta acgaccggcc tggcgcatca tccagacggg ggtgacatcg acgggctgct tgagcagggc gcggaggaag cggtcgttct tcagggcagt catatgg
Expected Protein Sequence
MAHHHHHHMT ALKNDRFLRA LLKQPVDVTP VWMMRQAGRY LPEYRATRAK AGDFMSLCMN PELACEVTLQ PLDRYPQLDA AILFSDILTI PDAMGQGLYF ETGEGPRFRK VVSSLADIEA LPVPDPEQDL GYVMDAVRTI RRELNGRVPL IGFSGSPWTL ATYMVEGGSS KDFRKSKAML YDNPKAMHAL LDKLAQSVTS YLNGQIHAGA QAVQIFDSWG GSLSAAAYQE FSLAYMRKIV DGLIREHDGR RVPVILFTKG GGLWLESMAE VGAEALGLDW TCDIGSARAR VGERVALQGN MDPSVLYANP AAIRAEVARI LAAYGKGTGH VFNLGHGITP EVDPAHAGAF FEAVHELSAQ YHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg ccctgaagaa cgaccgcttc ctccgcgccc tgctcaagca gcccgtcgat gtcacccccg tctggatgat gcgccaggcc ggtcgttacc tgccggaata ccgtgccacc cgtgccaagg ccggcgattt catgagcctg tgcatgaacc cggagctggc ctgcgaagtg accctgcagc cgctggaccg ttacccgcag ctggacgccg cgatcctctt ctccgatatc ctgaccattc ccgatgccat gggccagggc ctgtacttcg agaccggcga aggcccgcgc ttccgcaagg tggtctcgtc gctggccgac atcgaggcgc tgccggttcc cgatccggaa caggacctgg gctatgtgat ggacgccgtg cgcaccatcc gccgcgagct gaacggccgc gtaccgctga tcggcttctc cggcagcccc tggaccctcg ccacctacat ggtcgagggc ggctcgagca aggacttccg caagtccaag gcgatgctct acgacaaccc caaggccatg cacgctctgc tggacaagct ggcgcaatcg gtgaccagct acctcaacgg gcagatccac gccggcgccc aggcggtgca gatcttcgac tcatggggcg gcagcctgtc ggcggcggcc taccaggagt tctccctggc ctacatgcgc aagatcgtcg atggattgat ccgcgagcac gacggacgcc gggtgccggt gatcctcttc accaagggag gcggcctgtg gctggagtcg atggccgagg tcggcgccga ggcgctgggc ctggactgga cctgcgacat cggcagcgcc cgggcccgtg tcggtgagcg cgtagccctg caaggcaaca tggatccttc ggtgctctac gccaatccgg cggcgatccg cgccgaagtc gcgcggatcc tggcggccta cggcaagggt acggggcatg tgttcaatct cggccacggc atcaccccgg aagtcgaccc ggcccatgcc ggcgccttct tcgaggcggt gcacgagctg tcggcgcagt accacggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.01152.a.B1.PS02161
| PURIFICATION DATe: | 9/10/2014 |
| CONCENTRATION: | 65.81mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTALKNDRFL RALLKQPVDV TPVWMMRQAG RYLPEYRATR AKAGDFMSLC MNPELACEVT LQPLDRYPQL DAAILFSDIL TIPDAMGQGL YFETGEGPRF RKVVSSLADI EALPVPDPEQ DLGYVMDAVR TIRRELNGRV PLIGFSGSPW TLATYMVEGG SSKDFRKSKA MLYDNPKAMH ALLDKLAQSV TSYLNGQIHA GAQAVQIFDS WGGSLSAAAY QEFSLAYMRK IVDGLIREHD GRRVPVILFT KGGGLWLESM AEVGAEALGL DWTCDIGSAR ARVGERVALQ GNMDPSVLYA NPAAIRAEVA RILAAYGKGT GHVFNLGHGI TPEVDPAHAG AFFEAVHELS AQYH
Validated NT Sequence
cgtggtactg cgccgacagc tcgtgcaccg cctcgaagaa ggcgccggca tgggccgggt cgacttccgg ggtgatgccg tggccgagat tgaacacatg ccccgtaccc ttgccgtagg ccgccaggat ccgcgcgact tcggcgcgga tcgccgccgg attggcgtag agcaccgaag gatccatgtt gccttgcagg gctacgcgct caccgacacg ggcccgggcg ctgccgatgt cgcaggtcca gtccaggccc agcgcctcgg cgccgacctc ggccatcgac tccagccaca ggccgcctcc cttggtgaag aggatcaccg gcacccggcg tccgtcgtgc tcgcggatca atccatcgac gatcttgcgc atgtaggcca gggagaactc ctggtaggcc gccgccgaca ggctgccgcc ccatgagtcg aagatctgca ccgcctgggc gccggcgtgg atctgcccgt tgaggtagct ggtcaccgat tgcgccagct tgtccagcag agcgtgcatg gccttggggt tgtcgtagag catcgccttg gacttgcgga agtccttgct cgagccgccc tcgaccatgt aggtggcgag ggtccagggg ctgccggaga agccgatcag cggtacgcgg ccgttcagct cgcggcggat ggtgcgcacg gcgtccatca catagcccag gtcctgttcc ggatcgggaa ccggcagcgc ctcgatgtcg gccagcgacg agaccacctt gcggaagcgc gggccttcgc cggtctcgaa gtacaggccc tggcccatgg catcgggaat ggtcaggata tcggagaaga ggatcgcggc gtccagctgc gggtaacggt ccagcggctg cagggtcact tcgcaggcca gctccgggtt catgcacagg ctcatgaaat cgccggcctt ggcacgggtg gcacggtatt ccggcaggta acgaccggcc tggcgcatca tccagacggg ggtgacatcg acgggctgct tgagcagggc gcggaggaag cggtcgttct tcagggcagt catatgg
Expressed Protein Sequence
MAHHHHHHMT ALKNDRFLRA LLKQPVDVTP VWMMRQAGRY LPEYRATRAK AGDFMSLCMN PELACEVTLQ PLDRYPQLDA AILFSDILTI PDAMGQGLYF ETGEGPRFRK VVSSLADIEA LPVPDPEQDL GYVMDAVRTI RRELNGRVPL IGFSGSPWTL ATYMVEGGSS KDFRKSKAML YDNPKAMHAL LDKLAQSVTS YLNGQIHAGA QAVQIFDSWG GSLSAAAYQE FSLAYMRKIV DGLIREHDGR RVPVILFTKG GGLWLESMAE VGAEALGLDW TCDIGSARAR VGERVALQGN MDPSVLYANP AAIRAEVARI LAAYGKGTGH VFNLGHGITP EVDPAHAGAF FEAVHELSAQ YHG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG CCCTGAAGAA CGACCGCTTC CTCCGCGCCC TGCTCAAGCA GCCCGTCGAT GTCACCCCCG TCTGGATGAT GCGCCAGGCC GGTCGTTACC TGCCGGAATA CCGTGCCACC CGTGCCAAGG CCGGCGATTT CATGAGCCTG TGCATGAACC CGGAGCTGGC CTGCGAAGTG ACCCTGCAGC CGCTGGACCG TTACCCGCAG CTGGACGCCG CGATCCTCTT CTCCGATATC CTGACCATTC CCGATGCCAT GGGCCAGGGC CTGTACTTCG AGACCGGCGA AGGCCCGCGC TTCCGCAAGG TGGTCTCGTC GCTGGCCGAC ATCGAGGCGC TGCCGGTTCC CGATCCGGAA CAGGACCTGG GCTATGTGAT GGACGCCGTG CGCACCATCC GCCGCGAGCT GAACGGCCGC GTACCGCTGA TCGGCTTCTC CGGCAGCCCC TGGACCCTCG CCACCTACAT GGTCGAGGGC GGCTCGAGCA AGGACTTCCG CAAGTCCAAG GCGATGCTCT ACGACAACCC CAAGGCCATG CACGCTCTGC TGGACAAGCT GGCGCAATCG GTGACCAGCT ACCTCAACGG GCAGATCCAC GCCGGCGCCC AGGCGGTGCA GATCTTCGAC TCATGGGGCG GCAGCCTGTC GGCGGCGGCC TACCAGGAGT TCTCCCTGGC CTACATGCGC AAGATCGTCG ATGGATTGAT CCGCGAGCAC GACGGACGCC GGGTGCCGGT GATCCTCTTC ACCAAGGGAG GCGGCCTGTG GCTGGAGTCG ATGGCCGAGG TCGGCGCCGA GGCGCTGGGC CTGGACTGGA CCTGCGACAT CGGCAGCGCC CGGGCCCGTG TCGGTGAGCG CGTAGCCCTG CAAGGCAACA TGGATCCTTC GGTGCTCTAC GCCAATCCGG CGGCGATCCG CGCCGAAGTC GCGCGGATCC TGGCGGCCTA CGGCAAGGGT ACGGGGCATG TGTTCAATCT CGGCCACGGC ATCACCCCGG AAGTCGACCC GGCCCATGCC GGCGCCTTCT TCGAGGCGGT GCACGAGCTG TCGGCGCAGT ACCACGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT