SSGCID: Target Page

PsaeA.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP reductoisomerase) (EC 1.1.1.267) (1-deoxyxylulose-5-phosphate reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate synthase)

CENTER ID:	PsaeA.01136.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01136.a.B1.GE38270	full length		1	396

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01136.a.B1.PS02239	full length		1	396

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3819
PGAT:	pseudomonas:PA3650
RefSeq:	NP_252340.1
RefSeq:	WP_003113864.1
UniProt:	Q9KGU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRPQRISVL GATGSIGLST LDVVQRHPDR YEAFALTGFS RLAELEALCL RHRPVYAVVP EQAAAIALQG SLAAAGIRTR VLFGEQALCE VASAPEVDMV MAAIVGAAGL PSTLAAVEAG KRVLLANKEA LVMSGALFMQ AVKRSGAVLL PIDSEHNAIF QSLPRNYADG LERVGVRRIL LTASGGPFRE TPLEQLASVT PEQACAHPNW SMGRKISVDS ASMMNKGLEL IEACWLFDAQ PSQVEVVIHP QSVIHSMVDY VDGSVIAQLG NPDMRTPISY AMAWPERIDS GVSPLDMFAV GRLDFQRPDE QRFPCLRLAS QAAETGGSAP AMLNAANEVA VAAFLERHIR FSDIAVIIED VLNREAVTAV ESLDQVLAAD RRARSVAGQW LTRHAG

NT Sequence

atgagtcgac cgcagcggat cagcgtgctc ggcgcgaccg gctcgatcgg cctgagcacc ctggacgtcg tccagcgtca tcccgatcgt tacgaagcct tcgccctgac tggcttcagc cgcctggccg aactcgaggc gctgtgcctc aggcaccgcc ccgtctatgc ggtggtgccg gagcaggccg cggcgattgc cttgcagggc tcgctcgccg cggcgggtat ccgcacccgg gtgctgttcg gcgagcaggc gttgtgcgaa gtggccagcg cgcccgaagt ggacatggta atggcggcca tcgtcggcgc cgccgggctg ccgtcgaccc tggcggccgt cgaggccggc aagcgcgtac tgctggccaa caaggaggcg ctggtgatgt ccggcgcgct gttcatgcag gcggtcaagc gcagcggcgc ggtgctcctg ccgatcgaca gcgagcacaa cgcgatcttc cagtcgctgc cgcgcaatta tgccgatggc ctggagcggg tcggcgtgcg ccggatcctc ttgaccgcct ccggcggccc gttccgcgag acgccgctgg agcaactcgc ttcggtgacg ccggagcagg cttgtgcgca cccgaactgg tcgatggggc gtaagatttc cgtcgactcc gccagcatga tgaacaaggg gctcgaactg atcgaggcgt gctggctgtt cgacgcccag ccgagccagg tcgaggtggt gatccacccg cagagcgtga tccactcgat ggtggactac gtcgacggtt cggtgatcgc ccagctcggc aatccggaca tgcgcacgcc gatttcctat gccatggcct ggccggagcg aatcgattcc ggcgtttcgc cgctggatat gttcgccgtc ggtcgcctgg atttccagcg ccccgacgag cagcgcttcc cctgcctgcg cctggcgagc caggccgcgg aaaccggcgg cagcgccccg gccatgctga atgccgcgaa cgaggtggcc gtggccgcat ttctcgagcg gcacatccgc ttcagcgaca tcgcggttat catcgaggac gtgctgaacc gcgaggcggt gaccgcagtc gaatcgctcg atcaggtcct ggctgccgat cgccgcgcgc gttcggtcgc cgggcaatgg ttgacccggc acgccggcta g

Details for PsaeA.01136.a.B1.GE38270

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	94

Validated AA Sequence

MAHHHHHHMS RPQRISVLGA TGSIGLSTLD VVQRHPDRYE AFALTGFSRL AELEALCLRH RPVYAVVPEQ AAAIALQGSL AAAGIRTRVL FGEQALCEVA SAPEVDMVMA AIVGAAGLPS TLAAVEAGKR VLLANKEALV MSGALFMQAV KRSGAVLLPI DSEHNAIFQS LPRNYADGLE RVGVRRILLT ASGGPFRETP LEQLASVTPE QACAHPNWSM GRKISVDSAS MMNKGLELIE ACWLFDAQPS QVEVVIHPQS VIHSMVDYVD GSVIAQLGNP DMRTPISYAM AWPERIDSXV SPLDMFAVGR LDFQRPDEQR FPCLRLAXXA ETXAAPXXAX AXRXXXXISR XXIASXXSXX XXXXXXXTXX XXDXXXXXXX RX

Validated NT Sequence

cnctanatat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc gaccgcagcg gatcagcgtg ctcggcgcga ccggctcgat cggcctgagc accctggacg tcgtccagcg tcatcccgat cgttacgaag ccttcgccct gactggcttc agccgcctgg ccgaactcga ggcgctgtgc ctcaggcacc gccccgtcta tgcggtggtg ccggagcagg ccgcggcgat tgccttgcag ggctcgctcg ccgcggcggg tatccgcacc cgggtgctgt tcggcgagca ggcgttgtgc gaagtggcca gcgcgcccga agtggacatg gtaatggcgg ccatcgtcgg cgccgccggg ctgccgtcga ccctggcggc cgtcgaggcc ggcaagcgcg tactgctggc caacaaggag gcgctggtga tgtccggcgc gctgttcatg caggcggtca agcgcagcgg cgcggtgctc ctgccgatcg acagcgagca caacgcgatc ttccagtcgc tgccgcgcaa ttatgccgat ggcctggagc gggtcggcgt gcgccggatc ctcttgaccg cctccggcgg cccgttccgc gagacgccgc tggagcaact cgcttcggtg acgccggagc aggcttgtgc gcacccgaac tggtcgatgg ggcgtaagat ttccgtcgac tccgccagca tgatgaacaa ggggctcgaa ctgatcgagg cgtgctggct gttcgacgcc cagccgagcc aggtcgaggt ggtgatccac ccgcagagcg tgatccactc gatggtggac tacgtcgacg gttcggtgat cgcccagctc ggcaatccgg acatgcgcac gccgatttcc tatgccatgg cctggccgga gcgaatcgat tccnncgttt cgccgctgga tatgttcgcc gtcggtcgcc tggatttcca gcgccccgac gagcagcgct tcccctgcct gcgcctggcg annnnngcgg aaaccngcgc agcgccccnn nntgctgang cncnacgagn nnngtnnnnc atttctcgan cgnnnatcgc ttcannnnnn tcncnnntca tnnngnnnnn nnacgngnnn acncanncnn nnnnngatcn gnnngnnnna ntnnnncncc ggnang

Expected Protein Sequence

MAHHHHHHMS RPQRISVLGA TGSIGLSTLD VVQRHPDRYE AFALTGFSRL AELEALCLRH RPVYAVVPEQ AAAIALQGSL AAAGIRTRVL FGEQALCEVA SAPEVDMVMA AIVGAAGLPS TLAAVEAGKR VLLANKEALV MSGALFMQAV KRSGAVLLPI DSEHNAIFQS LPRNYADGLE RVGVRRILLT ASGGPFRETP LEQLASVTPE QACAHPNWSM GRKISVDSAS MMNKGLELIE ACWLFDAQPS QVEVVIHPQS VIHSMVDYVD GSVIAQLGNP DMRTPISYAM AWPERIDSGV SPLDMFAVGR LDFQRPDEQR FPCLRLASQA AETGGSAPAM LNAANEVAVA AFLERHIRFS DIAVIIEDVL NREAVTAVES LDQVLAADRR ARSVAGQWLT RHAG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc gaccgcagcg gatcagcgtg ctcggcgcga ccggctcgat cggcctgagc accctggacg tcgtccagcg tcatcccgat cgttacgaag ccttcgccct gactggcttc agccgcctgg ccgaactcga ggcgctgtgc ctcaggcacc gccccgtcta tgcggtggtg ccggagcagg ccgcggcgat tgccttgcag ggctcgctcg ccgcggcggg tatccgcacc cgggtgctgt tcggcgagca ggcgttgtgc gaagtggcca gcgcgcccga agtggacatg gtaatggcgg ccatcgtcgg cgccgccggg ctgccgtcga ccctggcggc cgtcgaggcc ggcaagcgcg tactgctggc caacaaggag gcgctggtga tgtccggcgc gctgttcatg caggcggtca agcgcagcgg cgcggtgctc ctgccgatcg acagcgagca caacgcgatc ttccagtcgc tgccgcgcaa ttatgccgat ggcctggagc gggtcggcgt gcgccggatc ctcttgaccg cctccggcgg cccgttccgc gagacgccgc tggagcaact cgcttcggtg acgccggagc aggcttgtgc gcacccgaac tggtcgatgg ggcgtaagat ttccgtcgac tccgccagca tgatgaacaa ggggctcgaa ctgatcgagg cgtgctggct gttcgacgcc cagccgagcc aggtcgaggt ggtgatccac ccgcagagcg tgatccactc gatggtggac tacgtcgacg gttcggtgat cgcccagctc ggcaatccgg acatgcgcac gccgatttcc tatgccatgg cctggccgga gcgaatcgat tccggcgttt cgccgctgga tatgttcgcc gtcggtcgcc tggatttcca gcgccccgac gagcagcgct tcccctgcct gcgcctggcg agccaggccg cggaaaccgg cggcagcgcc ccggccatgc tgaatgccgc gaacgaggtg gccgtggccg catttctcga gcggcacatc cgcttcagcg acatcgcggt tatcatcgag gacgtgctga accgcgaggc ggtgaccgca gtcgaatcgc tcgatcaggt cctggctgcc gatcgccgcg cgcgttcggt cgccgggcaa tggttgaccc ggcacgccgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for PsaeA.01136.a.B1.PS02239

PURIFICATION DATe:	12/4/2014
CONCENTRATION:	36.24mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	86
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMS RPQRISVLGA TGSIGLSTLD VVQRHPDRYE AFALTGFSRL AELEALCLRH RPVYAVVPEQ AAAIALQGSL AAAGIRTRVL FGEQALCEVA SAPEVDMVMA AIVGAAGLPS TLAAVEAGKR VLLANKEALV MSGALFMQAV KRSGAVLLPI DSEHNAIFQS LPRNYADGLE RVGVRRILLT ASGGPFRETP LEQLASVTPE QACAHPNWSM GRKISVDSAS MMNKGLELIE ACWLFDAQPS QVEVVIHPQS VIHSMVDYVD GSVIAQLGNP DMRTPISYAM AWPERIDSGV SPLDMFAVGR LDFQRPDEQR FPCLRLASQA AETGGSAPAM LNAANEVAVA AFLERHIRFS DIAVIIEDVL NREAVTAVES LDQVLAADRR ARSVAGQWLT RHAG

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTC GACCGCAGCG GATCAGCGTG CTCGGCGCGA CCGGCTCGAT CGGCCTGAGC ACCCTGGACG TCGTCCAGCG TCATCCCGAT CGTTACGAAG CCTTCGCCCT GACTGGCTTC AGCCGCCTGG CCGAACTCGA GGCGCTGTGC CTCAGGCACC GCCCCGTCTA TGCGGTGGTG CCGGAGCAGG CCGCGGCGAT TGCCTTGCAG GGCTCGCTCG CCGCGGCGGG TATCCGCACC CGGGTGCTGT TCGGCGAGCA GGCGTTGTGC GAAGTGGCCA GCGCGCCCGA AGTGGACATG GTAATGGCGG CCATCGTCGG CGCCGCCGGG CTGCCGTCGA CCCTGGCGGC CGTCGAGGCC GGCAAGCGCG TACTGCTGGC CAACAAGGAG GCGCTGGTGA TGTCCGGCGC GCTGTTCATG CAGGCGGTCA AGCGCAGCGG CGCGGTGCTC CTGCCGATCG ACAGCGAGCA CAACGCGATC TTCCAGTCGC TGCCGCGCAA TTATGCCGAT GGCCTGGAGC GGGTCGGCGT GCGCCGGATC CTCTTGACCG CCTCCGGCGG CCCGTTCCGC GAGACGCCGC TGGAGCAACT CGCTTCGGTG ACGCCGGAGC AGGCTTGTGC GCACCCGAAC TGGTCGATGG GGCGTAAGAT TTCCGTCGAC TCCGCCAGCA TGATGAACAA GGGGCTCGAA CTGATCGAGG CGTGCTGGCT GTTCGACGCC CAGCCGAGCC AGGTCGAGGT GGTGATCCAC CCGCAGAGCG TGATCCACTC GATGGTGGAC TACGTCGACG GTTCGGTGAT CGCCCAGCTC GGCAATCCGG ACATGCGCAC GCCGATTTCC TATGCCATGG CCTGGCCGGA GCGAATCGAT TCCGGCGTTT CGCCGCTGGA TATGTTCGCC GTCGGTCGCC TGGATTTCCA GCGCCCCGAC GAGCAGCGCT TCCCCTGCCT GCGCCTGGCG AGCCAGGCCG CGGAAACCGG CGGCAGCGCC CCGGCCATGC TGAATGCCGC GAACGAGGTG GCCGTGGCCG CATTTCTCGA GCGGCACATC CGCTTCAGCG ACATCGCGGT TATCATCGAG GACGTGCTGA ACCGCGAGGC GGTGACCGCA GTCGAATCGC TCGATCAGGT CCTGGCTGCC GATCGCCGCG CGCGTTCGGT CGCCGGGCAA TGGTTGACCC GGCACGCCGG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT