SSGCID: Target Page

PsaeA.01095.a

Octaprenyl-diphosphate synthase

CENTER ID:	PsaeA.01095.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01095.a.B1.GE38269	full length		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01095.a.B1.PS02163	full length		1	322

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4781
PGAT:	pseudomonas:PA4569
RefSeq:	NP_253259.1
UniProt:	Q9HVL5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQPQAFYRVV AEDFTAVDGI IRRQLTSRVP LVEKIGDYII SAGGKRLRPL LVLLAGKTLG YKGDDLCLLA ATIEFLHTST LLHDDVVDAS GLRRGRSTAN ALWGNAPSVL VGDFLYARSF EMMVELGSMP VMRIISQATR VIAEGEVLQL SKVRDASTTE ETYMEVIRGK TAMLFEASTH SAAALCQAGE EQSEALRRFG DYLGIAFQLV DDLLDYRGDA ATLGKNVGDD LAEGKPTLPL IVTMRDGTEE QAALVRKAIQ QGGSQDLESV CAAVEAAGAL DYTANLARDY AARAIACLDT LPDNEYRSAL VELSEFAVAR TH

NT Sequence

atgcaacccc aggccttcta ccgcgtggtg gcggaagatt tcaccgccgt cgacgggatc atccgtcgcc aactcacctc ccgcgttccc ctggtggaaa agatcggcga ctacatcatc tccgccggcg gcaagcgcct gcgtccgctg ctggtcctgc tcgccggcaa gaccctgggc tacaagggcg acgacctctg cctgctggcc gccaccatcg aattcctgca cacctccact ctgctgcacg acgacgtcgt cgacgcctcc ggcctgcgcc gcggccgctc caccgccaat gccctgtggg gcaacgcgcc gagcgtgctg gtaggcgact tcctttatgc ccgctccttc gaaatgatgg tcgagctggg ttccatgccg gtcatgcgca tcatatccca ggccacccgg gtgatcgccg agggcgaagt gctgcaattg tccaaggtac gcgacgccag cactaccgaa gagacctaca tggaagtgat ccgcggcaag accgcgatgc ttttcgaggc ctcgacccac agcgccgccg cgctgtgcca ggccggcgag gaacagtccg aggcactgcg ccgcttcggc gactacctgg gaatcgcctt ccagctggtg gacgacctgc tcgactaccg cggcgacgcc gcgaccctcg gcaagaacgt cggcgacgac ctcgccgaag gcaagcccac cctgccgctg atcgtcacca tgcgcgacgg caccgaggaa caggccgcgc tggtacgcaa agccatccag caaggtggca gccaggacct ggagagcgtc tgtgccgccg tcgaggcagc cggcgccctg gactataccg ccaacctcgc ccgcgactac gccgcccgcg ccatcgcctg cctggatacg ctgccggaca acgaataccg ctccgccctg gtcgaactca gcgaattcgc tgtcgcccga acccactga

Details for PsaeA.01095.a.B1.GE38269

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

atcttactca cttagtgggt tcgggcgaca gcgaattcgc tgagttcgac cagggcggag cggtattcgt tgtccggcag cgtatccagg caggcgatgg cgcgggcggc gtagtcgcgg gcgaggttgg cggtatagtc cagggcgccg gctgcctcga cggcggcaca gacgctctcc aggtcctggc tgccaccttg ctggatggct ttgcgtacca gcgcggcctg ttcctcggtg ccgtcgcgca tggtgacgat cagcggcagg gtgggcttgc cttcggcgag gtcgtcgccg acgttcttgc cgagggtcgc ggcgtcgccg cggtagtcga gcaggtcgtc caccagctgg aaggcgattc ccaggtagtc gccgaagcgg cgcagtgcct cggactgttc ctcgccggcc tggcacagcg cggcggcgct gtgggtcgag gcctcgaaaa gcatcgcggt cttgccgcgg atcacttcca tgtaggtctc ttcggtagtg ctggcgtcgc gtaccttgga caattgcagc acttcgccct cggcgatcac ccgggtggcc tgggatatga tgcgcatgac cggcatggaa cccagctcga ccatcatttc gaaggagcgg gcataaagga agtcgcctac cagcacgctc ggcgcgttgc cccacagggc attggcggtg gagcggccgc ggcgcaggcc ggaggcgtcg acgacgtcgt cgtgcagcag agtggaggtg tgcaggaatt cgatggtggc ggccagcagg cagaggtcgt cgcccttgta gcccagggtc ttgccggcga gcaggaccag cagcggacgc aggcgcttgc cgccggcgga gatgatgtag tcgccgatct tttccaccag gggaacgcgg gaggtgagtt ggcgacggat gatcccgtcg acggcggtga aatcttccgc caccacgcgg tagaaggcct ggggttgcat atg

Expected Protein Sequence

MAHHHHHHMQ PQAFYRVVAE DFTAVDGIIR RQLTSRVPLV EKIGDYIISA GGKRLRPLLV LLAGKTLGYK GDDLCLLAAT IEFLHTSTLL HDDVVDASGL RRGRSTANAL WGNAPSVLVG DFLYARSFEM MVELGSMPVM RIISQATRVI AEGEVLQLSK VRDASTTEET YMEVIRGKTA MLFEASTHSA AALCQAGEEQ SEALRRFGDY LGIAFQLVDD LLDYRGDAAT LGKNVGDDLA EGKPTLPLIV TMRDGTEEQA ALVRKAIQQG GSQDLESVCA AVEAAGALDY TANLARDYAA RAIACLDTLP DNEYRSALVE LSEFAVARTH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac cccaggcctt ctaccgcgtg gtggcggaag atttcaccgc cgtcgacggg atcatccgtc gccaactcac ctcccgcgtt cccctggtgg aaaagatcgg cgactacatc atctccgccg gcggcaagcg cctgcgtccg ctgctggtcc tgctcgccgg caagaccctg ggctacaagg gcgacgacct ctgcctgctg gccgccacca tcgaattcct gcacacctcc actctgctgc acgacgacgt cgtcgacgcc tccggcctgc gccgcggccg ctccaccgcc aatgccctgt ggggcaacgc gccgagcgtg ctggtaggcg acttccttta tgcccgctcc ttcgaaatga tggtcgagct gggttccatg ccggtcatgc gcatcatatc ccaggccacc cgggtgatcg ccgagggcga agtgctgcaa ttgtccaagg tacgcgacgc cagcactacc gaagagacct acatggaagt gatccgcggc aagaccgcga tgcttttcga ggcctcgacc cacagcgccg ccgcgctgtg ccaggccggc gaggaacagt ccgaggcact gcgccgcttc ggcgactacc tgggaatcgc cttccagctg gtggacgacc tgctcgacta ccgcggcgac gccgcgaccc tcggcaagaa cgtcggcgac gacctcgccg aaggcaagcc caccctgccg ctgatcgtca ccatgcgcga cggcaccgag gaacaggccg cgctggtacg caaagccatc cagcaaggtg gcagccagga cctggagagc gtctgtgccg ccgtcgaggc agccggcgcc ctggactata ccgccaacct cgcccgcgac tacgccgccc gcgccatcgc ctgcctggat acgctgccgg acaacgaata ccgctccgcc ctggtcgaac tcagcgaatt cgctgtcgcc cgaacccact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for PsaeA.01095.a.B1.PS02163

PURIFICATION DATe:	9/11/2014
CONCENTRATION:	54.4mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC CCCAGGCCTT CTACCGCGTG GTGGCGGAAG ATTTCACCGC CGTCGACGGG ATCATCCGTC GCCAACTCAC CTCCCGCGTT CCCCTGGTGG AAAAGATCGG CGACTACATC ATCTCCGCCG GCGGCAAGCG CCTGCGTCCG CTGCTGGTCC TGCTCGCCGG CAAGACCCTG GGCTACAAGG GCGACGACCT CTGCCTGCTG GCCGCCACCA TCGAATTCCT GCACACCTCC ACTCTGCTGC ACGACGACGT CGTCGACGCC TCCGGCCTGC GCCGCGGCCG CTCCACCGCC AATGCCCTGT GGGGCAACGC GCCGAGCGTG CTGGTAGGCG ACTTCCTTTA TGCCCGCTCC TTCGAAATGA TGGTCGAGCT GGGTTCCATG CCGGTCATGC GCATCATATC CCAGGCCACC CGGGTGATCG CCGAGGGCGA AGTGCTGCAA TTGTCCAAGG TACGCGACGC CAGCACTACC GAAGAGACCT ACATGGAAGT GATCCGCGGC AAGACCGCGA TGCTTTTCGA GGCCTCGACC CACAGCGCCG CCGCGCTGTG CCAGGCCGGC GAGGAACAGT CCGAGGCACT GCGCCGCTTC GGCGACTACC TGGGAATCGC CTTCCAGCTG GTGGACGACC TGCTCGACTA CCGCGGCGAC GCCGCGACCC TCGGCAAGAA CGTCGGCGAC GACCTCGCCG AAGGCAAGCC CACCCTGCCG CTGATCGTCA CCATGCGCGA CGGCACCGAG GAACAGGCCG CGCTGGTACG CAAAGCCATC CAGCAAGGTG GCAGCCAGGA CCTGGAGAGC GTCTGTGCCG CCGTCGAGGC AGCCGGCGCC CTGGACTATA CCGCCAACCT CGCCCGCGAC TACGCCGCCC GCGCCATCGC CTGCCTGGAT ACGCTGCCGG ACAACGAATA CCGCTCCGCC CTGGTCGAAC TCAGCGAATT CGCTGTCGCC CGAACCCACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT