PsaeA.01062.a

Dihydrofolate reductase (EC 1.5.1.3)

CENTER ID: PsaeA.01062.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.01062.a.B1.GE38267 full length 1 168
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.368
PGAT: pseudomonas:PA0350
RefSeq: NP_249041.1
UniProt: Q9I6E3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARPLAMIAA LGENRAIGID NRLPWRLPAD LKHFKAMTLG KPVIMGRKTW DSLGRPLPGR LNLVVSRQAG LALEGAEVFA SLDAALARAE AWAQAEDADE LMLIGGAQLY AEALPRAARL YLTRVGLAPE GDAFFPEIDG AAWRLASSIE HAAADDAPAY AFEVWERR
NT Sequence
atggcacgac cgctggcgat gatcgccgcc cttggcgaga accgcgcgat cggcatcgac aaccgcctgc cctggcgcct gccggccgac ctcaagcatt tcaaggcgat gaccctcggc aagccggtga tcatggggcg caagacctgg gactcgctgg gtcggccgct gccgggccgg ctaaacctgg tggtcagccg ccaggccggc ctggcactgg agggtgcgga agtgttcgcc agcctggacg ccgcgctggc gcgcgccgag gcgtgggcgc aggcggagga cgccgacgaa ctgatgctga tcggcggcgc ccagctctat gccgaggcct tgccgcgcgc cgcgcgtctc tacctgaccc gggttggcct ggcgccggag ggcgacgcct ttttccccga gatcgatggt gcggcctggc gcctggcgtc gagcatcgaa cacgccgcgg ctgacgatgc gcctgcctac gccttcgagg tatgggagcg ccgctga
Details for PsaeA.01062.a.B1.GE38267
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 19kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RPLAMIAALG ENRAIGIDNR LPWRLPADLK HFKAMTLGKP VIMGRKTWDS LGRPLPGRLN LVVSRQAGLA LEGAEVFASL DAALARAEAW AQAEDADELM LIGGAQLYAE ALPRAARLYL TRVGLAPEGD AFFPEIDGAA WRLASSIEHA AADDAPAYAF EVWERR
Validated NT Sequence
cnctagnnta ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggca cgaccgctgg cgatgatcgc cgcccttggc gagaaccgcg cgatcggcat cgacaaccgc ctgccctggc gcctgccggc cgacctcaag catttcaagg cgatgaccct cggcaagccg gtgatcatgg ggcgcaagac ctgggactcg ctgggtcggc cgctgccggg ccggctaaac ctggtggtca gccgccaggc cggcctggca ctggagggtg cggaagtgtt cgccagcctg gacgccgcgc tggcgcgcgc cgaggcgtgg gcgcaggcgg aggacgccga cgaactgatg ctgatcggcg gcgcccagct ctatgccgag gccttgccgc gcgccgcgcg tctctacctg acccgggttg gcctggcgcc ggagggcgac gcctttttcc ccgagatcga tggtgcggcc tggcgcctgg cgtcgagcat cgaacacgcc gcggctgacg atgcgcctgc ctacgccttc gaggtatggg agcgccgcta agtgagtaag ataggatccg gctgctaaca ncccccaaaa agaagaanna aagnttcggt gcgcaagacc gctgggcgaa atccgctagg aggtcgcctg catcatcgtc nagccggttg ccggcaacat gaactgtgtg ccgccagcgc ccgggcttcc tcggaaagnc tgcgcgaagc ctgcgacgaa ctcngcgtgg atgctgatcn gcnacgaaat gatgaccgnc gtccgtgtcc nctcncggcg cccatgccta ctacggggtc actcccggna cctgtcgacc ctcggcacga tcannngcgg cggcangcct ggtgggcgcc ttcagcggcn ncgcganatc atgcancana tatcccngct agtcccagtt cntaccaagg ccancnngct ctcnngnnac cnntggcant ggncggccgg acctgatcan nccgntcngc cttgattnnn nncncgccgn tttccg
Expected Protein Sequence
MAHHHHHHMA RPLAMIAALG ENRAIGIDNR LPWRLPADLK HFKAMTLGKP VIMGRKTWDS LGRPLPGRLN LVVSRQAGLA LEGAEVFASL DAALARAEAW AQAEDADELM LIGGAQLYAE ALPRAARLYL TRVGLAPEGD AFFPEIDGAA WRLASSIEHA AADDAPAYAF EVWERR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gaccgctggc gatgatcgcc gcccttggcg agaaccgcgc gatcggcatc gacaaccgcc tgccctggcg cctgccggcc gacctcaagc atttcaaggc gatgaccctc ggcaagccgg tgatcatggg gcgcaagacc tgggactcgc tgggtcggcc gctgccgggc cggctaaacc tggtggtcag ccgccaggcc ggcctggcac tggagggtgc ggaagtgttc gccagcctgg acgccgcgct ggcgcgcgcc gaggcgtggg cgcaggcgga ggacgccgac gaactgatgc tgatcggcgg cgcccagctc tatgccgagg ccttgccgcg cgccgcgcgt ctctacctga cccgggttgg cctggcgccg gagggcgacg cctttttccc cgagatcgat ggtgcggcct ggcgcctggc gtcgagcatc gaacacgccg cggctgacga tgcgcctgcc tacgccttcg aggtatggga gcgccgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at