SSGCID: Target Page

PsaeA.01028.a

tyrosyl tRNA synthetase

CENTER ID:	PsaeA.01028.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01028.a.B3.GE43990	truncation 2		1	317
PsaeA.01028.a.B4.GE43991	truncation 3		1	386

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4336
PGAT:	pseudomonas:PA4138
RefSeq:	WP_003113003.1
UniProt:	Q9HWP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVPTHQQDL IALLEERGFV HQCTDRDGLA AHLAAGPATA YLGFDATADS LHVGHLQGLM LMRWLQKAGH RPLLLIGGAT TRIGDPSFRD SSRPILTEAQ IQANIDGIAR VFSRYVELHD DSLVNNAEWL DGVGYLEFLD RVGRHFSINR LLTFDAIRQR LDREHSLSFL EFGYTLLQAY DFVELSRRRG CTLQLGGADQ WANIINGVEL SRRQGGAQLF GLTMPLLATS DGRKMGKSAQ GAVWLNAERL APFDFWQFWR NCDDRDVGRF LALFSELPMD EVRRLGALQG AELNEAKVVL ANAATALAHG EHAARSAADA ARGVFADGTR DSGLPVMKLS RARLAQGLSL TDLLLEHAIQ PSRSAVRRLA AGGGLRLDGT PVSDPDTPLA GEVDGLRLSL GKKQHLHLRL ED

NT Sequence

gtgtccgtcc cgacccatca gcaagacctg atcgccctgc tcgaagagcg tggcttcgtc caccagtgca ccgaccgcga cggcctcgcc gcgcacctgg ccgccggtcc ggcaaccgcc tacctgggct tcgacgccac cgccgacagc ctgcacgtcg gccacctaca gggcctgatg ctgatgcgct ggctacagaa ggccgggcac cgcccgttgc tgctgatcgg tggcgcgacc acccggatcg gcgatccgag cttccgcgat tcgagccggc cgatcctcac cgaggcgcag atccaggcca acatcgacgg catcgcccgg gttttctcgc gctacgtcga actgcacgac gacagcctgg tgaacaacgc cgaatggctg gacggcgtcg gctacctgga gttcctcgac cgggtcggcc ggcacttctc gatcaaccgc ctgctgacct tcgacgccat caggcagcgc ctggaccgcg agcattcgct gtcgttcctc gagttcggct acaccctgct gcaggcctac gatttcgtcg aactgtcgcg ccggcgcggc tgcaccctgc aactcggcgg cgccgaccag tgggcgaaca tcatcaacgg cgtggagctg tcgcgacgcc agggcggcgc ccagctgttc ggcctgacca tgccgctgct ggccaccagt gacgggcgca agatgggcaa gtcggcgcag ggtgcggtat ggctcaacgc cgagcgcctg gcgccgttcg acttctggca gttctggcgc aactgcgatg atcgcgacgt cggtcgcttc ctcgccctgt tcagcgaact gccgatggat gaggtgcgcc gcctgggcgc cttgcagggc gccgagctga acgaagcgaa ggtggtcctg gccaatgccg ccaccgccct ggcgcacggc gagcacgccg cgcgatccgc cgccgatgcc gcgcgcgggg tgttcgccga cggtacgcgg gacagcggcc tgccggtgat gaagctatcc cgcgcgcgcc tggcgcaggg actgtcgctg accgacctgc tgctggaaca cgcgatccag ccctcccgca gcgcggtccg ccgcctcgcc gccggcggcg gcctgcgcct ggacggcacg ccggtcagcg atccggacac gccgctggcg ggcgaggtcg acgggctgcg cttgagcctg ggcaagaagc agcatttgca cctgcgactc gaggattga

Details for PsaeA.01028.a.B3.GE43990

HARVESTED ON:	12/3/2020
SEQUENCED ON:	12/16/2020
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	79

Validated AA Sequence

MLMRWLQKAG HRPLLLIGGA TTRIGDPSFR DSSRPILTEA QIQANIDGIA RVFSRYVELH DDSLVNNAEW LDGVGYLEFL DRVGRHFSIN RLLTFDAIRQ RLDREHSLSF LEFGYTLLQA YDFVELSRRR GCTLQLGGAD QWANIINGVE LSRRQGGAQL FGLTMPLLAT SDGRKMGKSA QGAVWLNAER LAPFDFWQFW RNCDDRDVGR FLALFSELPM DEVRRLGALQ GAELNEAKVV LANAATALAH GEHAARSA

Validated NT Sequence

ttttcnnnnt ttgttagcag ccggnnccta tcttactcac ttaggcggat cgcgcggcgt gctcgccgtg cgccagggcg gtggcggcat tggccaggac caccttcgct tcgttcagct cggcgccctg caaggcgccc aggcggcgca cctcatccat cggcagttcg ctgaacaggg cgaggaagcg accgacgtcg cgatcatcgc agttgcgcca gaactgccag aagtcgaacg gcgccaggcg ctcggcgttg agccataccg caccctgcgc cgacttgccc atcttgcgcc cgtcactggt ggccagcagc ggcatggtca ggccgaacag ctgggcgccg ccctggcgtc gcgacagctc cacgccgttg atgatgttcg cccactggtc ggcgccgccg agttgcaggg tgcagccgcg ccggcgcgac agttcgacga aatcgtaggc ctgcagcagg gtgtagccga actcgaggaa cgacagcgaa tgctcgcggt ccaggcgctg cctgatggcg tcgaaggtca gcaggcggtt gatcgagaag tgccggccga cccggtcgag gaactccagg tagccgacgc cgtccagcca ttcggcgttg ttcaccaggc tgtcgtcgtg cagttcgacg tagcgcgaga aaacccgggc gatgccgtcg atgttggcct ggatctgcgc ctcggtgagg atcggccggc tcgaatcgcg gaagctcgga tcgccgatcc gggtggtcgc gccaccgatc agcagcaacg ggcggtgccc ggccttctgt agccagcgca tcagcatcag gccctgtnnt ggccgacgtg cngctgtcgg cggtggcgtc gaagcccagg taggcggntt gccggaccgg cggccaggtg cgcggcgang gccgtcgcgg tcggtgcact ggtggacgaa gccacgctct tcgagcnggn gatcaggtct tgctgatggg tcgggacgga cncatatgnn ngnggnnngn ggnngnnann nnngnnnana nctcgttcnt angnnnanac nnaantnttt tcnngangna aacccgntgg nng

Expected Protein Sequence

MAHHHHHHMS VPTHQQDLIA LLEERGFVHQ CTDRDGLAAH LAAGPATAYL GFDATADSLH VGHLQGLMLM RWLQKAGHRP LLLIGGATTR IGDPSFRDSS RPILTEAQIQ ANIDGIARVF SRYVELHDDS LVNNAEWLDG VGYLEFLDRV GRHFSINRLL TFDAIRQRLD REHSLSFLEF GYTLLQAYDF VELSRRRGCT LQLGGADQWA NIINGVELSR RQGGAQLFGL TMPLLATSDG RKMGKSAQGA VWLNAERLAP FDFWQFWRNC DDRDVGRFLA LFSELPMDEV RRLGALQGAE LNEAKVVLAN AATALAHGEH AARSA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtccg tcccgaccca tcagcaagac ctgatcgccc tgctcgaaga gcgtggcttc gtccaccagt gcaccgaccg cgacggcctc gccgcgcacc tggccgccgg tccggcaacc gcctacctgg gcttcgacgc caccgccgac agcctgcacg tcggccacct acagggcctg atgctgatgc gctggctaca gaaggccggg caccgcccgt tgctgctgat cggtggcgcg accacccgga tcggcgatcc gagcttccgc gattcgagcc ggccgatcct caccgaggcg cagatccagg ccaacatcga cggcatcgcc cgggttttct cgcgctacgt cgaactgcac gacgacagcc tggtgaacaa cgccgaatgg ctggacggcg tcggctacct ggagttcctc gaccgggtcg gccggcactt ctcgatcaac cgcctgctga ccttcgacgc catcaggcag cgcctggacc gcgagcattc gctgtcgttc ctcgagttcg gctacaccct gctgcaggcc tacgatttcg tcgaactgtc gcgccggcgc ggctgcaccc tgcaactcgg cggcgccgac cagtgggcga acatcatcaa cggcgtggag ctgtcgcgac gccagggcgg cgcccagctg ttcggcctga ccatgccgct gctggccacc agtgacgggc gcaagatggg caagtcggcg cagggtgcgg tatggctcaa cgccgagcgc ctggcgccgt tcgacttctg gcagttctgg cgcaactgcg atgatcgcga cgtcggtcgc ttcctcgccc tgttcagcga actgccgatg gatgaggtgc gccgcctggg cgccttgcag ggcgccgagc tgaacgaagc gaaggtggtc ctggccaatg ccgccaccgc cctggcgcac ggcgagcacg ccgcgcgatc cgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for PsaeA.01028.a.B4.GE43991

HARVESTED ON:	12/3/2020
SEQUENCED ON:	12/16/2020
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMV SVPTHQQDLI ALLEERGFVH QCTDRDGLAA HLAAGPATAY LGFDATADSL HVGHLQGLML MRWLQKAGHR PLLLIGGATT RIGDPSFRDS SRPILTEAQI QANIDGIARV FSRYVELHDD SLVNNAEWLD GVGYLEFLDR VGRHFSINRL LTFDAIRQRL DREHSLSFLE FGYTLLQAYD FVELSRRRGC TLQLGGADQW ANIINGVELS RRQGGAQLFG LTMPLLATSD GRKMGKSAQG AVWLNAERLA PFDFWQFWRN CDDRDVGRFL ALFSELPMDE VRRLGALQGA ELNEAKVVLA NAATALAHGE HAARSAADAA RGVFADGTRD SGLPVMKLSR ARLAQGLSLT DLLLEHAIQP SRSAVRRLAA GGGLRLDG

Validated NT Sequence

atggctcacc accaccacca ccatatggtg tccgtcccga cccatcagca agacctgatc gccctgctcg aagagcgtgg cttcgtccac cagtgcaccg accgcgacgg cctcgccgcg cacctggccg ccggtccggc aaccgcctac ctgggcttcg acgccaccgc cgacagcctg cacgtcggcc acctacaggg cctgatgctg atgcgctggc tacagaaggc cgggcaccgc ccgttgctgc tgatcggtgg cgcgaccacc cggatcggcg atccgagctt ccgcgattcg agccggccga tcctcaccga ggcgcagatc caggccaaca tcgacggcat cgcccgggtt ttctcgcgct acgtcgaact gcacgacgac agcctggtga acaacgccga atggctggac ggcgtcggct acctggagtt cctcgaccgg gtcggccggc acttctcgat caaccgcctg ctgaccttcg acgccatcag gcagcgcctg gaccgcgagc attcgctgtc gttcctcgag ttcggctaca ccctgctgca ggcctacgat ttcgtcgaac tgtcgcgccg gcgcggctgc accctgcaac tcggcggcgc cgaccagtgg gcgaacatca tcaacggcgt ggagctgtcg cgacgccagg gcggcgccca gctgttcggc ctgaccatgc cgctgctggc caccagtgac gggcgcaaga tgggcaagtc ggcgcagggt gcggtatggc tcaacgccga gcgcctggcg ccgttcgact tctggcagtt ctggcgcaac tgcgatgatc gcgacgtcgg tcgcttcctc gccctgttca gcgaactgcc gatggatgag gtgcgccgcc tgggcgcctt gcagggcgcc gagctgaacg aagcgaaggt ggtcctggcc aatgccgcca ccgccctggc gcacggcgag cacgccgcgc gatccgccgc cgatgccgcg cgcggggtgt tcgccgacgg tacgcgggac agcggcctgc cggtgatgaa gctatcccgc gcgcgcctgg cgcagggact gtcgctgacc gacctgctgc tggaacacgc gatccagccc tcccgcagcg cggtccgccg cctcgccgcc ggcggcggcc tgcgcctgga cggcacg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtccg tcccgaccca tcagcaagac ctgatcgccc tgctcgaaga gcgtggcttc gtccaccagt gcaccgaccg cgacggcctc gccgcgcacc tggccgccgg tccggcaacc gcctacctgg gcttcgacgc caccgccgac agcctgcacg tcggccacct acagggcctg atgctgatgc gctggctaca gaaggccggg caccgcccgt tgctgctgat cggtggcgcg accacccgga tcggcgatcc gagcttccgc gattcgagcc ggccgatcct caccgaggcg cagatccagg ccaacatcga cggcatcgcc cgggttttct cgcgctacgt cgaactgcac gacgacagcc tggtgaacaa cgccgaatgg ctggacggcg tcggctacct ggagttcctc gaccgggtcg gccggcactt ctcgatcaac cgcctgctga ccttcgacgc catcaggcag cgcctggacc gcgagcattc gctgtcgttc ctcgagttcg gctacaccct gctgcaggcc tacgatttcg tcgaactgtc gcgccggcgc ggctgcaccc tgcaactcgg cggcgccgac cagtgggcga acatcatcaa cggcgtggag ctgtcgcgac gccagggcgg cgcccagctg ttcggcctga ccatgccgct gctggccacc agtgacgggc gcaagatggg caagtcggcg cagggtgcgg tatggctcaa cgccgagcgc ctggcgccgt tcgacttctg gcagttctgg cgcaactgcg atgatcgcga cgtcggtcgc ttcctcgccc tgttcagcga actgccgatg gatgaggtgc gccgcctggg cgccttgcag ggcgccgagc tgaacgaagc gaaggtggtc ctggccaatg ccgccaccgc cctggcgcac ggcgagcacg ccgcgcgatc cgccgccgat gccgcgcgcg gggtgttcgc cgacggtacg cgggacagcg gcctgccggt gatgaagcta tcccgcgcgc gcctggcgca gggactgtcg ctgaccgacc tgctgctgga acacgcgatc cagccctccc gcagcgcggt ccgccgcctc gccgccggcg gcggcctgcg cctggacggc acgccggtca gcgatccgga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat