PsaeA.01026.a
Glutamate-1-semialdehyde 2,1-aminomutase (GSA) (EC 5.4.3.8) (Glutamate-1-semialdehyde aminotransferase) (GSA-AT)
| CENTER ID: | PsaeA.01026.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.01026.a.B1.GE38266 | full length | 1 | 427 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5I92
DEPOSITED: 2/19/2016
DETERMINATION: XRay
CLONE: PsaeA.01026.a.B1.GE38266
PROTEIN: PsaeA.01026.a.B1.PS02194
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4169 |
| PGAT: | pseudomonas:PA3977 |
| RefSeq: | NP_252666.1 |
| UniProt: | P48247 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRSETLFNN AQKHIPGGVN SPVRAFKSVG GTPLFFKHAE GAYVLDEDDK RYVDYVGSWG PMILGHSHPD VLDAVRRQLD HGLSYGAPTA LEVEMADLVC SMVPSMEMVR MVSSGTEATM SAIRLARGYT GRDSIIKFEG CYHGHSDSLL VKAGSGALTF GVPNSPGVPA AFAKHTLTLP FNDIEAVRKT LGEVGKEVAC IIVEPVAGNM NCVPPAPGFL EGLREACDEH GVVLIFDEVM TGFRVALGGA QAYYGVTPDL STFGKIIGGG MPVGAFGGKR EIMQQISPLG PVYQAGTLSG NPLAMAAGLT TLRLISRPGF HDELTAYTTR MLDGLQQRAD AAGIPFVTTQ AGGMFGLYFS GADAIVTFED VMASDVERFK RFFHLMLDGG VYLAPSAFEA GFTSIAHGDK ELEITLNAAE KAFAALK
NT Sequence
atgtcccgtt ccgaaacgct gttcaacaac gcccagaaac acattcccgg cggggtcaac tccccggtgc gcgccttcaa gagcgtcggc ggcaccccgc tgttcttcaa gcacgccgaa ggcgcctatg tactggacga ggacgacaag cgctacgtgg actatgtcgg ctcctggggc ccgatgatcc tcggccacag ccatccggac gtgctcgacg cggtgcgccg gcaactcgac cacggcctgt cctatggcgc gccgaccgcg ctggaagtgg aaatggccga cctggtctgc tccatggtgc cgtcgatgga gatggtgcgc atggtcagct ccggcaccga ggcgaccatg agcgccatcc gcctggcccg cggctacacc gggcgcgaca gcatcatcaa gttcgaaggc tgctaccacg gtcactccga cagcctgctg gtgaaggccg gctccggctc cctgaccttc ggcgtgccga actccccggg cgttccggcg gccttcgcca agcacaccct gaccctgccg ttcaacgaca tcgaggcggt gcgcaagacc ctgggcgaag tcggcaagga ggtcgcctgc atcatcgtcg agccggttgc cggcaacatg aactgtgtgc cgccggcgcc gggcttcctc gaaggcctgc gcgaagcctg cgacgaacac ggcgtggtgc tgatcttcga cgaagtgatg accggcttcc gtgtcgccct cggcggcgcc caggcctact acggggtcac cccggacctg tcgaccttcg gcaagatcat cggcggcggc atgccggtgg gcgccttcgg cggcaagcgc gagatcatgc agcagatttc cccgctgggc ccggtctacc aggccggcac gctctcgggc aacccgctgg caatggccgc cggcctgacc accctgcgcc tgatcagccg ccccggtttc cacgacgaac tgaccgccta caccacgcgc atgctcgacg gcctgcaaca gcgcgcggat gccgccggca tccccttcgt caccacccag gcgggcggca tgttcggcct gtacttcagc ggcgccgacg ccatcgtcac cttcgaggac gtgatggcca gcgacgtcga acgcttcaag cgcttcttcc acctgatgct cgacggtggc gtgtaccttg cgcctagcgc cttcgaggcc ggcttcacct ccatcgccca tggcgacaag gagctggaaa tcacgctgaa cgccgccgaa aaagccttcg ccgcgctgaa atga
Details for PsaeA.01026.a.B1.GE38266
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 21 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MGDGGEAGLE GARRKVHATV EHQVEEALEA FDVAGHHVLE GDDGVGAAEV QAEHAARLGG DEGDAGGIRA LLQAVEHARG VGGQFVVETG AADQAQGGQA GGHCQRVARE RAGLVDRAQR GNLLHDLALA AEGAHRHAAA DDLAEGRQVR GDPVVGLGAA EGDTEAGHHF VEDQHHAVFV AGFAQAFEEA RRRRHTVHVA GNRLDDDAGD LLADFAQGLA HRLDVVERQG QGVLGEGRRN ARGVRHAEGQ GAGAGLHQQA VGVTVVAAFE LDDAVAPGVA AGQADGAHGR LGAGADHAHH LHRRHHGADQ VGHFHFQRGR RAIGQAVVEL PAHRVEHVRM AVAEDHRAPG ADIVHVALVV LVQYIGAFGV LEEQRGAADA LEGAHRGVDP AGNVFLGVVE QRFG
Validated NT Sequence
cggcggcgtt cagcgtgatt tccagctcct tgtcgccatg ggcgatggag gtgaagccgg cctcgaaggc gctaggcgca aggtacacgc caccgtcgag catcaggtgg aagaagcgct tgaagcgttc gacgtcgctg gccatcacgt cctcgaaggt gacgatggcg tcggcgccgc tgaagtacag gccgaacatg ccgcccgcct gggtggtgac gaaggggatg ccggcggcat ccgcgcgctg ttgcaggccg tcgagcatgc gcgtggtgta ggcggtcagt tcgtcgtgga aaccggggcg gctgatcagg cgcagggtgg tcaggccggc ggccattgcc agcgggttgc ccgagagcgt gccggcctgg tagaccgggc ccagcgggga aatctgctgc atgatctcgc gcttgccgcc gaaggcgccc accggcatgc cgccgccgat gatcttgccg aaggtcgaca ggtccggggt gaccccgtag taggcctggg cgccgccgag ggcgacacgg aagccggtca tcacttcgtc gaagatcagc accacgccgt gttcgtcgca ggcttcgcgc aggccttcga ggaagcccgg cgccggcggc acacagttca tgttgccggc aaccggctcg acgatgatgc aggcgacctc cttgccgact tcgcccaggg tcttgcgcac cgcctcgatg tcgttgaacg gcagggtcag ggtgtgcttg gcgaaggccg ccggaacgcc cggggagttc ggcacgccga aggtcagggc gccggagccg gccttcacca gcaggctgtc ggagtgaccg tggtagcagc cttcgaactt gatgatgctg tcgcgcccgg tgtagccgcg ggccaggcgg atggcgctca tggtcgcctc ggtgccggag ctgaccatgc gcaccatctc catcgacggc accatggagc agaccaggtc ggccatttcc acttccagcg cggtcggcgc gccataggac aggccgtggt cgagttgccg gcgcaccgcg tcgagcacgt ccggatggct gtggccgagg atcatcgggc cccaggagcc gacatagtcc acgtagcgct tgtcgtcctc gtccagtaca taggcgcctt cggcgtgctt gaagaacagc ggggtgccgc cgacgctctt gaaggcgcgc accggggagt tgaccccgcc gggaatgtgt ttctgggcgt tgttgaacag cgtttcggaa c
Expected Protein Sequence
MAHHHHHHMS RSETLFNNAQ KHIPGGVNSP VRAFKSVGGT PLFFKHAEGA YVLDEDDKRY VDYVGSWGPM ILGHSHPDVL DAVRRQLDHG LSYGAPTALE VEMADLVCSM VPSMEMVRMV SSGTEATMSA IRLARGYTGR DSIIKFEGCY HGHSDSLLVK AGSGALTFGV PNSPGVPAAF AKHTLTLPFN DIEAVRKTLG EVGKEVACII VEPVAGNMNC VPPAPGFLEG LREACDEHGV VLIFDEVMTG FRVALGGAQA YYGVTPDLST FGKIIGGGMP VGAFGGKREI MQQISPLGPV YQAGTLSGNP LAMAAGLTTL RLISRPGFHD ELTAYTTRML DGLQQRADAA GIPFVTTQAG GMFGLYFSGA DAIVTFEDVM ASDVERFKRF FHLMLDGGVY LAPSAFEAGF TSIAHGDKEL EITLNAAEKA FAALK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccc gttccgaaac gctgttcaac aacgcccaga aacacattcc cggcggggtc aactccccgg tgcgcgcctt caagagcgtc ggcggcaccc cgctgttctt caagcacgcc gaaggcgcct atgtactgga cgaggacgac aagcgctacg tggactatgt cggctcctgg ggcccgatga tcctcggcca cagccatccg gacgtgctcg acgcggtgcg ccggcaactc gaccacggcc tgtcctatgg cgcgccgacc gcgctggaag tggaaatggc cgacctggtc tgctccatgg tgccgtcgat ggagatggtg cgcatggtca gctccggcac cgaggcgacc atgagcgcca tccgcctggc ccgcggctac accgggcgcg acagcatcat caagttcgaa ggctgctacc acggtcactc cgacagcctg ctggtgaagg ccggctccgg ctccctgacc ttcggcgtgc cgaactcccc gggcgttccg gcggccttcg ccaagcacac cctgaccctg ccgttcaacg acatcgaggc ggtgcgcaag accctgggcg aagtcggcaa ggaggtcgcc tgcatcatcg tcgagccggt tgccggcaac atgaactgtg tgccgccggc gccgggcttc ctcgaaggcc tgcgcgaagc ctgcgacgaa cacggcgtgg tgctgatctt cgacgaagtg atgaccggct tccgtgtcgc cctcggcggc gcccaggcct actacggggt caccccggac ctgtcgacct tcggcaagat catcggcggc ggcatgccgg tgggcgcctt cggcggcaag cgcgagatca tgcagcagat ttccccgctg ggcccggtct accaggccgg cacgctctcg ggcaacccgc tggcaatggc cgccggcctg accaccctgc gcctgatcag ccgccccggt ttccacgacg aactgaccgc ctacaccacg cgcatgctcg acggcctgca acagcgcgcg gatgccgccg gcatcccctt cgtcaccacc caggcgggcg gcatgttcgg cctgtacttc agcggcgccg acgccatcgt caccttcgag gacgtgatgg ccagcgacgt cgaacgcttc aagcgcttct tccacctgat gctcgacggt ggcgtgtacc ttgcgcctag cgccttcgag gccggcttca cctccatcgc ccatggcgac aaggagctgg aaatcacgct gaacgccgcc gaaaaagcct tcgccgcgct gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat