SSGCID: Target Page

PsaeA.01012.a

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (Asp/Glu-ADT subunit B) (EC 6.3.5.-)

CENTER ID:	PsaeA.01012.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01012.a.B1.GE38264	full length		1	481

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.01012.a.B1.PS02153	full length		1	481

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4694
PGAT:	pseudomonas:PA4484
RefSeq:	NP_253174.1
UniProt:	Q9HVT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQWETVIGLE IHAQLATQSK IFSGSSTAFG AAPNTQASLV DLAMPGTLPV LNEEAVRMAC LFGLAIDARI DRQNVFARKN YFYPDLPKGY QTSQMDHPIV GKGHLDITLE DGTTKRIGIT RAHLEEDAGK SLHEDFQGMS GIDLNRAGTP LLEIVSEPDI RSAKEAVAYV KAIHALVRYL GICDGNMAEG SLRCDCNVSV RPKGQAEFGT RAEIKNVNSF RFIEKAINHE IQRQIELIED GGKVVQETRL YDPNKDETRS MRGKEEANDY RYFPCPDLLP VVIEPEYLAK LREQLPELPV QKRERFESQY GLSAYDASVL SASREMADYF EKVQGICGDA KLAANWVMVE LGSLLNKDGL EIEQSPVSAE QLGGMILRIK DNTISGKLAK MVFEAMANGE GSADQIIEAK GLKQVTDSGA IEKMLDEVLA ANAEQVEQYR AADEAKRGKM FGFFVGQAMK ASKGKANPQQ VNELLKKKLE A

NT Sequence

atgcaatggg aaaccgtgat cgggctggaa atccacgcac agctcgccac ccaatcgaag atcttctccg gcagctcgac cgccttcggc gccgcgccga atacccaagc cagcctggtc gacctggcca tgccgggcac cctgccggtg ctcaacgagg aggccgtacg catggcctgc ctgttcggcc tggcgatcga cgcacggatc gaccggcaga acgtgttcgc ccgcaagaac tacttctacc ccgacctgcc gaagggctac cagaccagcc agatggacca tcctatcgtc ggcaagggcc atctcgacat caccctggaa gacggcacca ccaagcgcat cggcatcacc cgcgcgcacc tggaggagga cgccggcaag agcctgcacg aagacttcca gggcatgagc ggcatcgacc tgaaccgcgc cggcacgccg ttgctggaga tcgtttccga gccggacatc cgcagcgcca aggaagccgt ggcctacgtc aaggcgatcc acgccctggt gcgctacctg ggcatctgcg acggcaacat ggccgagggc tcgctgcgct gcgactgcaa cgtctcggta cgaccgaagg gccaggccga attcggcacc cgcgccgaga tcaagaacgt caactccttc cgcttcatcg agaaggcgat caaccacgag atccagcgcc agatcgagct gatcgaggac ggcggcaagg tagtccagga aacccgcctg tacgacccga acaaggacga gacccgctcc atgcgcggca aggaagaagc caacgactac cgttacttcc cctgtcccga cctgctgccg gtggtcatcg agcccgagta cctggcgaag ctgcgcgagc aactgccgga actgccggtg cagaagcgcg aacgcttcga gagccagtac ggcctgtccg cctacgatgc cagcgtgctg tccgccagcc gcgagatggc cgactacttc gagaaggtcc agggcatctg cggcgacgcc aagctggcgg ccaactgggt gatggtcgag ctgggcagcc tgctcaacaa ggacggcctg gagatcgagc agtccccggt gtccgccgag caactgggcg gcatgatcct gcgcatcaag gacaacacca tctccggcaa gctcgccaag atggtcttcg aagccatggc caacggcgag ggttccgccg accagatcat cgaggccaag ggcctgaagc aggtcaccga cagtggcgcc atcgagaaga tgctcgacga ggtgctggcg gccaacgccg agcaggtcga gcagtaccgc gctgccgacg aggccaagcg cggcaagatg ttcggcttct tcgtcggcca ggcgatgaag gcctcgaaag gcaaggccaa cccgcagcag gtgaacgagt tgctgaagaa aaagctcgaa gcgtga

Details for PsaeA.01012.a.B1.GE38264

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/10/2014
EXPECTED MW:	54kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	21
PERCENT COVERAGE:	96

Validated AA Sequence

MGNRDRAGNP RTARHPIEDL LRQLDRLRRR AEYPSQPGRP GHAGHPAGAQ RGGRTHGLPV RPGDRRTDRP AERVRPQELL LPRPAEGLPD QPDGPSYRRQ GPSRHHPGRR HHQAHRHHPR APGGGRRQEP ARRLPGHERH RPEPRRHAVA GDRFRAGHPQ RQGSRGLRQG DPRPGALPGH LRRQHGRGLA ALRLQRLGTT EGPGRIRHPR RDQERQLLPL HREGDQPRDP APDRADRGRR QGSPGNPPVR PEQGRDPLHA RQGRSQRLPL LPLSRPAAGG HRARVPGEAA RATAGTAGAE ARTLREPVRP VRLRCQRAVR QPRDGRLLRE GPGHLRRRQA GGQLGDGRAG QPAQQGRPGD RAVPGVRRAT GRHDPAHQGQ HHLRQARQDG LRSHGQRRGF RRPDHRGQGP EAGHRQWRHR EDARRGAGGQ RRAGRAVPRC RRGQARQDVR LLRRPGDEGL ERQGQPAAGE R

Validated NT Sequence

aactcgttca cctgctgcgg gttggccttg cctttcgagg ccttcatcgc ctggccgacg aagaagccga acatcttgcc gcgcttggcc tcgtcggcag cgcggtactg ctcgacctgc tcggcgttgg ccgccagcac ctcgtcgagc atcttctcga tggcgccact gtcggtgacc tgcttcaggc ccttggcctc gatgatctgg tcggcggaac cctcgccgtt ggccatggct tcgaagacca tcttggcgag cttgccggag atggtgttgt ccttgatgcg caggatcatg ccgcccagtt gctcggcgga caccggggac tgctcgatct ccaggccgtc cttgttgagc aggctgccca gctcgaccat cacccagttg gccgccagct tggcgtcgcc gcagatgccc tggaccttct cgaagtagtc ggccatctcg cggctggcgg acagcacgct ggcatcgtag gcggacaggc cgtactggct ctcgaagcgt tcgcgcttct gcaccggcag ttccggcagt tgctcgcgca gcttcgccag gtactcgggc tcgatgacca ccggcagcag gtcgggacag gggaagtaac ggtagtcgtt ggcttcttcc ttgccgcgca tggagcgggt ctcgtccttg ttcgggtcgt acaggcgggt ttcctggact accttgccgc cgtcctcgat cagctcgatc tggcgctgga tctcgtggtt gatcgccttc tcgatgaagc ggaaggagtt gacgttcttg atctcggcgc gggtgccgaa ttcggcctgg cccttcggtc gtaccgagac gttgcagtcg cagcgcagcg agccctcggc catgttgccg tcgcagatgc ccaggtagcg caccagggcg tggatcgcct tgacgtaggc cacggcttcc ttggcgctgc ggatgtccgg ctcggaaacg atctccagca acggcgtgcc ggcgcggttc aggtcgatgc cgctcatgcc ctggaagtct tcgtgcaggc tcttgccggc gtcctcctcc aggtgcgcgc gggtgatgcc gatgcgcttg gtggtgccgt cttccagggt gatgtcgaga tggcccttgc cgacgatagg atggtccatc tggctggtct ggtagccctt cggcaggtcg gggtagaagt agttcttgcg ggcgaacacg ttctgccggt cgatccgtgc gtcgatcgcc aggccgaaca ggcaggccat gcgtacggcc tcctcgttga gcaccggcag ggtgcccggc atggccaggt cgaccaggct ggcttgggta ttcggcgcgg cgccgaaggc ggtcgagctg ccggagaaga tcttcgattg ggtggcgagc tgtgcgtgga tttccagccc gatcacggtt tcccattg

Expected Protein Sequence

MAHHHHHHMQ WETVIGLEIH AQLATQSKIF SGSSTAFGAA PNTQASLVDL AMPGTLPVLN EEAVRMACLF GLAIDARIDR QNVFARKNYF YPDLPKGYQT SQMDHPIVGK GHLDITLEDG TTKRIGITRA HLEEDAGKSL HEDFQGMSGI DLNRAGTPLL EIVSEPDIRS AKEAVAYVKA IHALVRYLGI CDGNMAEGSL RCDCNVSVRP KGQAEFGTRA EIKNVNSFRF IEKAINHEIQ RQIELIEDGG KVVQETRLYD PNKDETRSMR GKEEANDYRY FPCPDLLPVV IEPEYLAKLR EQLPELPVQK RERFESQYGL SAYDASVLSA SREMADYFEK VQGICGDAKL AANWVMVELG SLLNKDGLEI EQSPVSAEQL GGMILRIKDN TISGKLAKMV FEAMANGEGS ADQIIEAKGL KQVTDSGAIE KMLDEVLAAN AEQVEQYRAA DEAKRGKMFG FFVGQAMKAS KGKANPQQVN ELLKKKLEA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat gggaaaccgt gatcgggctg gaaatccacg cacagctcgc cacccaatcg aagatcttct ccggcagctc gaccgccttc ggcgccgcgc cgaataccca agccagcctg gtcgacctgg ccatgccggg caccctgccg gtgctcaacg aggaggccgt acgcatggcc tgcctgttcg gcctggcgat cgacgcacgg atcgaccggc agaacgtgtt cgcccgcaag aactacttct accccgacct gccgaagggc taccagacca gccagatgga ccatcctatc gtcggcaagg gccatctcga catcaccctg gaagacggca ccaccaagcg catcggcatc acccgcgcgc acctggagga ggacgccggc aagagcctgc acgaagactt ccagggcatg agcggcatcg acctgaaccg cgccggcacg ccgttgctgg agatcgtttc cgagccggac atccgcagcg ccaaggaagc cgtggcctac gtcaaggcga tccacgccct ggtgcgctac ctgggcatct gcgacggcaa catggccgag ggctcgctgc gctgcgactg caacgtctcg gtacgaccga agggccaggc cgaattcggc acccgcgccg agatcaagaa cgtcaactcc ttccgcttca tcgagaaggc gatcaaccac gagatccagc gccagatcga gctgatcgag gacggcggca aggtagtcca ggaaacccgc ctgtacgacc cgaacaagga cgagacccgc tccatgcgcg gcaaggaaga agccaacgac taccgttact tcccctgtcc cgacctgctg ccggtggtca tcgagcccga gtacctggcg aagctgcgcg agcaactgcc ggaactgccg gtgcagaagc gcgaacgctt cgagagccag tacggcctgt ccgcctacga tgccagcgtg ctgtccgcca gccgcgagat ggccgactac ttcgagaagg tccagggcat ctgcggcgac gccaagctgg cggccaactg ggtgatggtc gagctgggca gcctgctcaa caaggacggc ctggagatcg agcagtcccc ggtgtccgcc gagcaactgg gcggcatgat cctgcgcatc aaggacaaca ccatctccgg caagctcgcc aagatggtct tcgaagccat ggccaacggc gagggttccg ccgaccagat catcgaggcc aagggcctga agcaggtcac cgacagtggc gccatcgaga agatgctcga cgaggtgctg gcggccaacg ccgagcaggt cgagcagtac cgcgctgccg acgaggccaa gcgcggcaag atgttcggct tcttcgtcgg ccaggcgatg aaggcctcga aaggcaaggc caacccgcag caggtgaacg agttgctgaa gaaaaagctc gaagcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for PsaeA.01012.a.B1.PS02153

PURIFICATION DATe:	9/4/2014
CONCENTRATION:	37.68mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	21
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAT GGGAAACCGT GATCGGGCTG GAAATCCACG CACAGCTCGC CACCCAATCG AAGATCTTCT CCGGCAGCTC GACCGCCTTC GGCGCCGCGC CGAATACCCA AGCCAGCCTG GTCGACCTGG CCATGCCGGG CACCCTGCCG GTGCTCAACG AGGAGGCCGT ACGCATGGCC TGCCTGTTCG GCCTGGCGAT CGACGCACGG ATCGACCGGC AGAACGTGTT CGCCCGCAAG AACTACTTCT ACCCCGACCT GCCGAAGGGC TACCAGACCA GCCAGATGGA CCATCCTATC GTCGGCAAGG GCCATCTCGA CATCACCCTG GAAGACGGCA CCACCAAGCG CATCGGCATC ACCCGCGCGC ACCTGGAGGA GGACGCCGGC AAGAGCCTGC ACGAAGACTT CCAGGGCATG AGCGGCATCG ACCTGAACCG CGCCGGCACG CCGTTGCTGG AGATCGTTTC CGAGCCGGAC ATCCGCAGCG CCAAGGAAGC CGTGGCCTAC GTCAAGGCGA TCCACGCCCT GGTGCGCTAC CTGGGCATCT GCGACGGCAA CATGGCCGAG GGCTCGCTGC GCTGCGACTG CAACGTCTCG GTACGACCGA AGGGCCAGGC CGAATTCGGC ACCCGCGCCG AGATCAAGAA CGTCAACTCC TTCCGCTTCA TCGAGAAGGC GATCAACCAC GAGATCCAGC GCCAGATCGA GCTGATCGAG GACGGCGGCA AGGTAGTCCA GGAAACCCGC CTGTACGACC CGAACAAGGA CGAGACCCGC TCCATGCGCG GCAAGGAAGA AGCCAACGAC TACCGTTACT TCCCCTGTCC CGACCTGCTG CCGGTGGTCA TCGAGCCCGA GTACCTGGCG AAGCTGCGCG AGCAACTGCC GGAACTGCCG GTGCAGAAGC GCGAACGCTT CGAGAGCCAG TACGGCCTGT CCGCCTACGA TGCCAGCGTG CTGTCCGCCA GCCGCGAGAT GGCCGACTAC TTCGAGAAGG TCCAGGGCAT CTGCGGCGAC GCCAAGCTGG CGGCCAACTG GGTGATGGTC GAGCTGGGCA GCCTGCTCAA CAAGGACGGC CTGGAGATCG AGCAGTCCCC GGTGTCCGCC GAGCAACTGG GCGGCATGAT CCTGCGCATC AAGGACAACA CCATCTCCGG CAAGCTCGCC AAGATGGTCT TCGAAGCCAT GGCCAACGGC GAGGGTTCCG CCGACCAGAT CATCGAGGCC AAGGGCCTGA AGCAGGTCAC CGACAGTGGC GCCATCGAGA AGATGCTCGA CGAGGTGCTG GCGGCCAACG CCGAGCAGGT CGAGCAGTAC CGCGCTGCCG ACGAGGCCAA GCGCGGCAAG ATGTTCGGCT TCTTCGTCGG CCAGGCGATG AAGGCCTCGA AAGGCAAGGC CAACCCGCAG CAGGTGAACG AGTTGCTGAA GAAAAAGCTC GAAGCGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T