PsaeA.00980.a
Phosphoserine aminotransferase (EC 2.6.1.52) (Phosphohydroxythreonine aminotransferase) (PSAT)
| CENTER ID: | PsaeA.00980.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00980.a.B1.GE38263 | full length | 1 | 361 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00980.a.B1.PW37585 | full length | 1 | 361 |
Structures
4XK1
DEPOSITED: 1/9/2015
DETERMINATION: XRay
CLONE: PsaeA.00980.a.B1.GE38263
PROTEIN: PsaeA.00980.a.B1.PW37585
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.3310 |
| PGAT: | pseudomonas:PA3167 |
| RefSeq: | NP_251857.1 |
| UniProt: | Q9HZ66 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKRAFNFCA GPAALPDAVL QRAQAELLDW RGKGLSVMEM SHRSDDYVAI ASKAEQDLRD LLDIPSDYKV LFLQGGASQQ FAEIPLNLLP EDGVADYIDT GIWSKKAIEE ARRYGTVNVA ASAKEYDYFA IPGQNEWTLT KDAAYVHYAS NETIGGLEFD WIPETGDVPL VTDMSSDILS RPLDVSRFGL IYAGAQKNIG PSGLVVVIVR EDLLGRARSV CPTMLNYKTA ADNGSMYNTP ATYSWYLSGL VFEWLKEQGG VTAMEQRNRA KKDLLYKTID ASDFYTNPIQ PSARSWMNVP FRLADERLDK PFLEGAEARG LLNLKGHRSV GGMRASIYNA LGLDAVEALV AYMAEFEKEH G
NT Sequence
gtgagcaagc gagccttcaa tttctgcgcc ggtcccgcgg cgcttcccga cgcagttctg caacgtgccc aggccgagct tctcgactgg cggggcaagg gcctttcggt catggaaatg agccaccgta gtgatgacta cgtggccatc gccagcaagg ccgagcagga cctgcgcgac ctgctcgaca ttccctcgga ctacaaggtg ctgttcctgc agggcggcgc cagccagcag ttcgccgaga ttccgctgaa cctgctgccc gaagatggcg tcgccgacta tatcgatacc ggtatctggt cgaagaaggc catcgaggag gctcgtcgct atggcaccgt gaatgtcgcg gccagcgcca aggagtacga ctacttcgcc attccgggtc agaacgagtg gacgctgacc aaggacgcgg cctatgtgca ctacgcctcc aacgagacca tcggcggcct cgagttcgac tggattcccg agaccggcga cgtgccgctg gtcaccgaca tgtcctccga catcctctcg cgcccgctcg acgtgtcccg cttcggcctg atctatgccg gggcgcagaa gaacatcggc ccgtccggcc tggtggtggt gatcgttcgc gaagacctgc tcggccgcgc ccgcagcgtc tgtccgacca tgctcaacta caagaccgcc gcggacaacg gttccatgta caacaccccg gcgacttact cctggtacct gtccggcctg gtcttcgaat ggctgaagga gcagggcggg gtgaccgcga tggagcagcg caaccgcgcc aagaaggacc tgctgtacaa gaccatcgat gccagcgact tctacaccaa cccgatccag ccgagcgccc gctcctggat gaacgtgccg ttccgcctgg ccgacgagcg tctcgacaag ccgttcctgg aaggcgccga ggcgcgcggg ctgctcaacc tgaaaggcca ccgctcggtc ggcggcatgc gcgcttccat ctacaacgcc cttggcctgg acgcggtcga ggcgctggtc gcatacatgg cggagttcga gaaggagcac ggctga
Details for PsaeA.00980.a.B1.GE38263
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 81 |
| PERCENT COVERAGE: | 104 |
Validated AA Sequence
MAHHHHHHMV SKRAFNFCAG PAALPDAVLQ RAQAELLDWR GKGLSVMEMS HRSDDYVAIA SKAEQDLRDL LDIPSDYKVL FLQGGASQQF AEIPLNLLPE DGVADYIDTG IWSKKAIEEA RRYGTVNVAA SAKEYDYFAI PGQNEWTLTK DAAYVHYASN ETIGGLEFDW IPETGDVPLV TDMSSDILSR PLDVSRFGLI YAGAQKNIGP SGLVVVIVRE DLLGRARSVC PTMLNYKTAA DNGSMYNTPA TYSWYLSGLV FEWLKEXGGV TAMEQRNRAK KGPAVQDHRC QRLLHQPDPA ERPLLDERAV PPGRRASRQA VPGXRRGXRA AQPEKPPLRS AACXLPSTTX XPGXXEALSA YMAXXXXXXX XXXXXLXXXR PXXX
Validated NT Sequence
annnttttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgagcaag cgagccttca atttctgcgc cggtcccgcg gcgcttcccg acgcagttct gcaacgtgcc caggccgagc ttctcgactg gcggggcaag ggcctttcgg tcatggaaat gagccaccgt agtgatgact acgtggccat cgccagcaag gccgagcagg acctgcgcga cctgctcgac attccctcgg actacaaggt gctgttcctg cagggcggcg ccagccagca gttcgccgag attccgctga acctgctgcc cgaagatggc gtcgccgact atatcgatac cggtatctgg tcgaagaagg ccatcgagga ggctcgtcgc tatggcaccg tgaatgtcgc ggccagcgcc aaggagtacg actacttcgc cattccgggt cagaacgagt ggacgctgac caaggacgcg gcctatgtgc actacgcctc caacgagacc atcggcggcc tcgagttcga ctggattccc gagaccggcg acgtgccgct ggtcaccgac atgtcctccg acatcctctc gcgcccgctc gacgtgtccc gcttcggcct gatctatgcc ggggcgcaga agaacatcgg cccgtccggc ctggtggtgg tgatcgttcg cgaagacctg ctcggccgcg cccgcagcgt ctgtccgacc atgctcaact acaagaccgc cgcggacaac ggttccatgt acaacacccc ggcgacttac tcctggtacc tgtccggcct ggtcttcgaa tggctgaagg agcanggcgg ggtgaccgcg atggagcagc gcaaccgcgc caagaaagga cctgctgtac aagaccatcg atgccagcga cttctacacc aacccgatcc agccgagcgc ccgctcctgg atgaacgtgc cgttccgcct ggccgacgag cgtctcgaca agccgttcct ggaangcgcc gaggcncgcg ggctgctcaa cctgaaaagc caccgctncg gtcggcggcn tgcncgcttc catctacaac gcnnnggcct ggnngcgncg aggcgctgtc cgcatacatg gcgnnnnnan nnnnnngntn nngnntaana nnnnatncgc tgcnnnacan nagacccncn nagannaa
Expected Protein Sequence
MAHHHHHHMS KRAFNFCAGP AALPDAVLQR AQAELLDWRG KGLSVMEMSH RSDDYVAIAS KAEQDLRDLL DIPSDYKVLF LQGGASQQFA EIPLNLLPED GVADYIDTGI WSKKAIEEAR RYGTVNVAAS AKEYDYFAIP GQNEWTLTKD AAYVHYASNE TIGGLEFDWI PETGDVPLVT DMSSDILSRP LDVSRFGLIY AGAQKNIGPS GLVVVIVRED LLGRARSVCP TMLNYKTAAD NGSMYNTPAT YSWYLSGLVF EWLKEQGGVT AMEQRNRAKK DLLYKTIDAS DFYTNPIQPS ARSWMNVPFR LADERLDKPF LEGAEARGLL NLKGHRSVGG MRASIYNALG LDAVEALVAY MAEFEKEHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagca agcgagcctt caatttctgc gccggtcccg cggcgcttcc cgacgcagtt ctgcaacgtg cccaggccga gcttctcgac tggcggggca agggcctttc ggtcatggaa atgagccacc gtagtgatga ctacgtggcc atcgccagca aggccgagca ggacctgcgc gacctgctcg acattccctc ggactacaag gtgctgttcc tgcagggcgg cgccagccag cagttcgccg agattccgct gaacctgctg cccgaagatg gcgtcgccga ctatatcgat accggtatct ggtcgaagaa ggccatcgag gaggctcgtc gctatggcac cgtgaatgtc gcggccagcg ccaaggagta cgactacttc gccattccgg gtcagaacga gtggacgctg accaaggacg cggcctatgt gcactacgcc tccaacgaga ccatcggcgg cctcgagttc gactggattc ccgagaccgg cgacgtgccg ctggtcaccg acatgtcctc cgacatcctc tcgcgcccgc tcgacgtgtc ccgcttcggc ctgatctatg ccggggcgca gaagaacatc ggcccgtccg gcctggtggt ggtgatcgtt cgcgaagacc tgctcggccg cgcccgcagc gtctgtccga ccatgctcaa ctacaagacc gccgcggaca acggttccat gtacaacacc ccggcgactt actcctggta cctgtccggc ctggtcttcg aatggctgaa ggagcagggc ggggtgaccg cgatggagca gcgcaaccgc gccaagaagg acctgctgta caagaccatc gatgccagcg acttctacac caacccgatc cagccgagcg cccgctcctg gatgaacgtg ccgttccgcc tggccgacga gcgtctcgac aagccgttcc tggaaggcgc cgaggcgcgc gggctgctca acctgaaagg ccaccgctcg gtcggcggca tgcgcgcttc catctacaac gcccttggcc tggacgcggt cgaggcgctg gtcgcataca tggcggagtt cgagaaggag cacggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00980.a.B1.PW37585
| PURIFICATION DATe: | 11/4/2014 |
| CONCENTRATION: | 22mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 81 |
| PERCENT COVERAGE: | 104 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV SKRAFNFCAG PAALPDAVLQ RAQAELLDWR GKGLSVMEMS HRSDDYVAIA SKAEQDLRDL LDIPSDYKVL FLQGGASQQF AEIPLNLLPE DGVADYIDTG IWSKKAIEEA RRYGTVNVAA SAKEYDYFAI PGQNEWTLTK DAAYVHYASN ETIGGLEFDW IPETGDVPLV TDMSSDILSR PLDVSRFGLI YAGAQKNIGP SGLVVVIVRE DLLGRARSVC PTMLNYKTAA DNGSMYNTPA TYSWYLSGLV FEWLKEXGGV TAMEQRNRAK KGPAVQDHRC QRLLHQPDPA ERPLLDERAV PPGRRASRQA VPGXRRGXRA AQPEKPPLRS AACXLPSTTX XPGXXEALSA YMAXXXXXXX XXXXXLXXXR PXXX
Validated NT Sequence
annnttttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgagcaag cgagccttca atttctgcgc cggtcccgcg gcgcttcccg acgcagttct gcaacgtgcc caggccgagc ttctcgactg gcggggcaag ggcctttcgg tcatggaaat gagccaccgt agtgatgact acgtggccat cgccagcaag gccgagcagg acctgcgcga cctgctcgac attccctcgg actacaaggt gctgttcctg cagggcggcg ccagccagca gttcgccgag attccgctga acctgctgcc cgaagatggc gtcgccgact atatcgatac cggtatctgg tcgaagaagg ccatcgagga ggctcgtcgc tatggcaccg tgaatgtcgc ggccagcgcc aaggagtacg actacttcgc cattccgggt cagaacgagt ggacgctgac caaggacgcg gcctatgtgc actacgcctc caacgagacc atcggcggcc tcgagttcga ctggattccc gagaccggcg acgtgccgct ggtcaccgac atgtcctccg acatcctctc gcgcccgctc gacgtgtccc gcttcggcct gatctatgcc ggggcgcaga agaacatcgg cccgtccggc ctggtggtgg tgatcgttcg cgaagacctg ctcggccgcg cccgcagcgt ctgtccgacc atgctcaact acaagaccgc cgcggacaac ggttccatgt acaacacccc ggcgacttac tcctggtacc tgtccggcct ggtcttcgaa tggctgaagg agcanggcgg ggtgaccgcg atggagcagc gcaaccgcgc caagaaagga cctgctgtac aagaccatcg atgccagcga cttctacacc aacccgatcc agccgagcgc ccgctcctgg atgaacgtgc cgttccgcct ggccgacgag cgtctcgaca agccgttcct ggaangcgcc gaggcncgcg ggctgctcaa cctgaaaagc caccgctncg gtcggcggcn tgcncgcttc catctacaac gcnnnggcct ggnngcgncg aggcgctgtc cgcatacatg gcgnnnnnan nnnnnngntn nngnntaana nnnnatncgc tgcnnnacan nagacccncn nagannaa
Expressed Protein Sequence
MAHHHHHHMS KRAFNFCAGP AALPDAVLQR AQAELLDWRG KGLSVMEMSH RSDDYVAIAS KAEQDLRDLL DIPSDYKVLF LQGGASQQFA EIPLNLLPED GVADYIDTGI WSKKAIEEAR RYGTVNVAAS AKEYDYFAIP GQNEWTLTKD AAYVHYASNE TIGGLEFDWI PETGDVPLVT DMSSDILSRP LDVSRFGLIY AGAQKNIGPS GLVVVIVRED LLGRARSVCP TMLNYKTAAD NGSMYNTPAT YSWYLSGLVF EWLKEQGGVT AMEQRNRAKK DLLYKTIDAS DFYTNPIQPS ARSWMNVPFR LADERLDKPF LEGAEARGLL NLKGHRSVGG MRASIYNALG LDAVEALVAY MAEFEKEHG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCA AGCGAGCCTT CAATTTCTGC GCCGGTCCCG CGGCGCTTCC CGACGCAGTT CTGCAACGTG CCCAGGCCGA GCTTCTCGAC TGGCGGGGCA AGGGCCTTTC GGTCATGGAA ATGAGCCACC GTAGTGATGA CTACGTGGCC ATCGCCAGCA AGGCCGAGCA GGACCTGCGC GACCTGCTCG ACATTCCCTC GGACTACAAG GTGCTGTTCC TGCAGGGCGG CGCCAGCCAG CAGTTCGCCG AGATTCCGCT GAACCTGCTG CCCGAAGATG GCGTCGCCGA CTATATCGAT ACCGGTATCT GGTCGAAGAA GGCCATCGAG GAGGCTCGTC GCTATGGCAC CGTGAATGTC GCGGCCAGCG CCAAGGAGTA CGACTACTTC GCCATTCCGG GTCAGAACGA GTGGACGCTG ACCAAGGACG CGGCCTATGT GCACTACGCC TCCAACGAGA CCATCGGCGG CCTCGAGTTC GACTGGATTC CCGAGACCGG CGACGTGCCG CTGGTCACCG ACATGTCCTC CGACATCCTC TCGCGCCCGC TCGACGTGTC CCGCTTCGGC CTGATCTATG CCGGGGCGCA GAAGAACATC GGCCCGTCCG GCCTGGTGGT GGTGATCGTT CGCGAAGACC TGCTCGGCCG CGCCCGCAGC GTCTGTCCGA CCATGCTCAA CTACAAGACC GCCGCGGACA ACGGTTCCAT GTACAACACC CCGGCGACTT ACTCCTGGTA CCTGTCCGGC CTGGTCTTCG AATGGCTGAA GGAGCAGGGC GGGGTGACCG CGATGGAGCA GCGCAACCGC GCCAAGAAGG ACCTGCTGTA CAAGACCATC GATGCCAGCG ACTTCTACAC CAACCCGATC CAGCCGAGCG CCCGCTCCTG GATGAACGTG CCGTTCCGCC TGGCCGACGA GCGTCTCGAC AAGCCGTTCC TGGAAGGCGC CGAGGCGCGC GGGCTGCTCA ACCTGAAAGG CCACCGCTCG GTCGGCGGCA TGCGCGCTTC CATCTACAAC GCCCTTGGCC TGGACGCGGT CGAGGCGCTG GTCGCATACA TGGCGGAGTT CGAGAAGGAG CACGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T