PsaeA.00952.a
UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) (Enoylpyruvate transferase) (UDP-N-acetylglucosamine enolpyruvyl transferase) (EPT)
| CENTER ID: | PsaeA.00952.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00952.a.B1.GE38260 | full length | 1 | 421 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00952.a.B1.PS02212 | full length | 1 | 421 |
Structures
5BQ2
DEPOSITED: 5/28/2015
DETERMINATION: XRay
CLONE: PsaeA.00952.a.B1.GE38260
PROTEIN: PsaeA.00952.a.B1.PS02212
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4660 |
| PGAT: | pseudomonas:PA4450 |
| RefSeq: | NP_253140.1 |
| UniProt: | Q9HVW7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKLIITGGN RLDGEIRISG AKNSALPILA ATLLADTPVT VCNLPHLHDI TTMIELFGRM GVQPIIDEKL NVEVDASSIK TLVAPYELVK TMRASILVLG PMLARFGEAE VALPGGCAIG SRPVDLHIRG LEAMGAQIEV EGGYIKAKAP AGGLRGGHFF FDTVSVTGTE NLMMAAALAN GRTVLQNAAR EPEVVDLANC LNAMGANVQG AGSDTIVIEG VKRLGGARYD VLPDRIETGT YLVAAAATGG RVKLKDTDPT ILEAVLQKLE EAGAHISTGS NWIELDMKGN RPKAVNVRTA PYPAFPTDMQ AQFISMNAVA EGTGAVIETV FENRFMHVYE MNRMGAQILV EGNTAIVTGV PKLKGAPVMA TDLRASASLV IAGLVAEGDT LIDRIYHIDR GYECIEEKLQ LLGAKIRRVP G
NT Sequence
atggataaac tgattattac cggcggtaac cgcctcgatg gcgaaatccg catttccggc gcgaagaact cggcgctgcc gatcctggcc gcgaccctgc tggccgatac tccggtcacc gtctgcaacc tgccgcacct gcacgacatt accaccatga tcgaactgtt cggccgcatg ggcgtgcagc cgatcatcga cgagaagctc aacgtcgaag tcgatgccag cagcatcaaa accctggtcg cgccgtacga actggtgaag accatgcgtg cctcgatcct ggtgctgggc ccgatgctgg cgcgcttcgg cgaggccgaa gtggccctgc cgggcggttg cgcgatcggt tcgcgtccgg tcgacctgca tatccgcggt ctcgaggcca tgggcgcgca gatcgaggtc gaaggcggct acatcaaggc caaggcgccg gccggcggcc tgcgtggcgg tcacttcttc ttcgataccg tcagcgtgac cggcaccgag aacctgatga tggccgccgc gctggccaac ggccgtaccg tgctgcagaa cgccgctcgc gagccggagg tggtcgacct ggccaactgc ctgaacgcca tgggcgccaa cgtccagggc gctggctccg ataccatcgt catcgaaggc gtgaagcgcc tcggcggtgc tcgctacgac gtactgcccg accgtatcga gaccggcacc tacctggtgg ccgcggccgc gaccggtggc cgggtgaagc tgaaggatac cgacccgacc atcctcgagg cggtcctgca gaagctggaa gaggccggtg cccacatcag caccggcagc aactggatcg agctggacat gaagggcaac cggccgaagg cggtcaacgt gcgtaccgcg ccgtacccgg cgttccccac cgacatgcag gcccagttca tctccatgaa cgcggtagcc gaaggcaccg gcgcggtcat cgagacggtc ttcgagaacc gcttcatgca tgtttacgaa atgaaccgca tgggcgcgca aatcctcgtc gaaggcaaca ccgccatcgt caccggcgta cccaagctca agggcgctcc ggtcatggcg accgacctgc gcgcatccgc gagcctggtg atcgccggcc tggtggccga aggcgacacc ctgatcgatc gcatctacca catcgaccgt ggctacgagt gcatcgaaga gaaactccag ctgctcggcg ccaagatccg ccgcgtaccg ggctag
Details for PsaeA.00952.a.B1.GE38260
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Validated AA Sequence
MIELFGRMGV QPIIDEKLNV EVDASSIKTL VAPYELVKTM RASILVLGPM LARFGEAEVA LPGGCAIGSR PVDLHIRGLE AMGAQIEVEG GYIKAKAPAG GLRGGHFFFD TVSVTGTENL MMAAALANGR TVLQNAAREP EVVDLANCLN AMGANVQGAG SDTIVIEGVK RLGGARYDVL PDRIETGTYL VAAAATGGRV KLKDTDPTIL EAVLQKLEEA GAHISTGSNW IELDMKGNRP KAVNVRTAPY PAFPTDMQAQ FISMNAVAEG TGAVIETVFE NRFMHVYEMN RMGAQILVEG NTAIVTGVPK LKGAPVMATD LRASASLVIA GLVAEGDTLI DRIYHIDRGY ECIEEKLQLL G
Validated NT Sequence
cgccgagcag ctggagtttc tcttcgatgc actcgtagcc acggtcgatg tggtagatgc gatcgatcag ggtgtcgcct tcggccacca ggccggcgat caccaggctc gcggatgcgc gcaggtcggt cgccatgacc ggagcgccct tgagcttggg tacgccggtg acgatggcgg tgttgccttc gacgaggatt tgcgcgccca tgcggttcat ttcgtaaaca tgcatgaagc ggttctcgaa gaccgtctcg atgaccgcgc cggtgccttc ggctaccgcg ttcatggaga tgaactgggc ctgcatgtcg gtggggaacg ccgggtacgg cgcggtacgc acgttgaccg ccttcggccg gttgcccttc atgtccagct cgatccagtt gctgccggtg ctgatgtggg caccggcctc ttccagcttc tgcaggaccg cctcgaggat ggtcgggtcg gtatccttca gcttcacccg gccaccggtc gcggccgcgg ccaccaggta ggtgccggtc tcgatacggt cgggcagtac gtcgtagcga gcaccgccga ggcgcttcac gccttcgatg acgatggtat cggagccagc gccctggacg ttggcgccca tggcgttcag gcagttggcc aggtcgacca cctccggctc gcgagcggcg ttctgcagca cggtacggcc gttggccagc gcggcggcca tcatcaggtt ctcggtgccg gtcacgctga cggtatcgaa gaagaagtga ccgccacgca ggccgccggc cggcgccttg gccttgatgt agccgccttc gacctcgatc tgcgcgccca tggcctcgag accgcggata tgcaggtcga ccggacgcga accgatcgcg caaccgcccg gcagggccac ttcggcctcg ccgaagcgcg ccagcatcgg gcccagcacc aggatcgagg cacgcatggt cttcaccagt tcgtacggcg cgaccagggt tttgatgctg ctggcatcga cttcgacgtt gagcttctcg tcgatgatcg gctgcacgcc catgcggccg aacagttcga tcatggtggt aatgtcgtgc aggtgcggca ggttgcagac ggtgaccgga gtatcggcca gcagggtcgc ggccaggatc ggcagcgccg agttcttcgc gccggaaatg cggatttcgc catcgaggcg gttaccgccg gtaataatca gtttatcc
Expected Protein Sequence
MAHHHHHHMD KLIITGGNRL DGEIRISGAK NSALPILAAT LLADTPVTVC NLPHLHDITT MIELFGRMGV QPIIDEKLNV EVDASSIKTL VAPYELVKTM RASILVLGPM LARFGEAEVA LPGGCAIGSR PVDLHIRGLE AMGAQIEVEG GYIKAKAPAG GLRGGHFFFD TVSVTGTENL MMAAALANGR TVLQNAAREP EVVDLANCLN AMGANVQGAG SDTIVIEGVK RLGGARYDVL PDRIETGTYL VAAAATGGRV KLKDTDPTIL EAVLQKLEEA GAHISTGSNW IELDMKGNRP KAVNVRTAPY PAFPTDMQAQ FISMNAVAEG TGAVIETVFE NRFMHVYEMN RMGAQILVEG NTAIVTGVPK LKGAPVMATD LRASASLVIA GLVAEGDTLI DRIYHIDRGY ECIEEKLQLL GAKIRRVPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata aactgattat taccggcggt aaccgcctcg atggcgaaat ccgcatttcc ggcgcgaaga actcggcgct gccgatcctg gccgcgaccc tgctggccga tactccggtc accgtctgca acctgccgca cctgcacgac attaccacca tgatcgaact gttcggccgc atgggcgtgc agccgatcat cgacgagaag ctcaacgtcg aagtcgatgc cagcagcatc aaaaccctgg tcgcgccgta cgaactggtg aagaccatgc gtgcctcgat cctggtgctg ggcccgatgc tggcgcgctt cggcgaggcc gaagtggccc tgccgggcgg ttgcgcgatc ggttcgcgtc cggtcgacct gcatatccgc ggtctcgagg ccatgggcgc gcagatcgag gtcgaaggcg gctacatcaa ggccaaggcg ccggccggcg gcctgcgtgg cggtcacttc ttcttcgata ccgtcagcgt gaccggcacc gagaacctga tgatggccgc cgcgctggcc aacggccgta ccgtgctgca gaacgccgct cgcgagccgg aggtggtcga cctggccaac tgcctgaacg ccatgggcgc caacgtccag ggcgctggct ccgataccat cgtcatcgaa ggcgtgaagc gcctcggcgg tgctcgctac gacgtactgc ccgaccgtat cgagaccggc acctacctgg tggccgcggc cgcgaccggt ggccgggtga agctgaagga taccgacccg accatcctcg aggcggtcct gcagaagctg gaagaggccg gtgcccacat cagcaccggc agcaactgga tcgagctgga catgaagggc aaccggccga aggcggtcaa cgtgcgtacc gcgccgtacc cggcgttccc caccgacatg caggcccagt tcatctccat gaacgcggta gccgaaggca ccggcgcggt catcgagacg gtcttcgaga accgcttcat gcatgtttac gaaatgaacc gcatgggcgc gcaaatcctc gtcgaaggca acaccgccat cgtcaccggc gtacccaagc tcaagggcgc tccggtcatg gcgaccgacc tgcgcgcatc cgcgagcctg gtgatcgccg gcctggtggc cgaaggcgac accctgatcg atcgcatcta ccacatcgac cgtggctacg agtgcatcga agagaaactc cagctgctcg gcgccaagat ccgccgcgta ccgggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00952.a.B1.PS02212
| PURIFICATION DATe: | 11/19/2014 |
| CONCENTRATION: | 56.53mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MIELFGRMGV QPIIDEKLNV EVDASSIKTL VAPYELVKTM RASILVLGPM LARFGEAEVA LPGGCAIGSR PVDLHIRGLE AMGAQIEVEG GYIKAKAPAG GLRGGHFFFD TVSVTGTENL MMAAALANGR TVLQNAAREP EVVDLANCLN AMGANVQGAG SDTIVIEGVK RLGGARYDVL PDRIETGTYL VAAAATGGRV KLKDTDPTIL EAVLQKLEEA GAHISTGSNW IELDMKGNRP KAVNVRTAPY PAFPTDMQAQ FISMNAVAEG TGAVIETVFE NRFMHVYEMN RMGAQILVEG NTAIVTGVPK LKGAPVMATD LRASASLVIA GLVAEGDTLI DRIYHIDRGY ECIEEKLQLL G
Validated NT Sequence
cgccgagcag ctggagtttc tcttcgatgc actcgtagcc acggtcgatg tggtagatgc gatcgatcag ggtgtcgcct tcggccacca ggccggcgat caccaggctc gcggatgcgc gcaggtcggt cgccatgacc ggagcgccct tgagcttggg tacgccggtg acgatggcgg tgttgccttc gacgaggatt tgcgcgccca tgcggttcat ttcgtaaaca tgcatgaagc ggttctcgaa gaccgtctcg atgaccgcgc cggtgccttc ggctaccgcg ttcatggaga tgaactgggc ctgcatgtcg gtggggaacg ccgggtacgg cgcggtacgc acgttgaccg ccttcggccg gttgcccttc atgtccagct cgatccagtt gctgccggtg ctgatgtggg caccggcctc ttccagcttc tgcaggaccg cctcgaggat ggtcgggtcg gtatccttca gcttcacccg gccaccggtc gcggccgcgg ccaccaggta ggtgccggtc tcgatacggt cgggcagtac gtcgtagcga gcaccgccga ggcgcttcac gccttcgatg acgatggtat cggagccagc gccctggacg ttggcgccca tggcgttcag gcagttggcc aggtcgacca cctccggctc gcgagcggcg ttctgcagca cggtacggcc gttggccagc gcggcggcca tcatcaggtt ctcggtgccg gtcacgctga cggtatcgaa gaagaagtga ccgccacgca ggccgccggc cggcgccttg gccttgatgt agccgccttc gacctcgatc tgcgcgccca tggcctcgag accgcggata tgcaggtcga ccggacgcga accgatcgcg caaccgcccg gcagggccac ttcggcctcg ccgaagcgcg ccagcatcgg gcccagcacc aggatcgagg cacgcatggt cttcaccagt tcgtacggcg cgaccagggt tttgatgctg ctggcatcga cttcgacgtt gagcttctcg tcgatgatcg gctgcacgcc catgcggccg aacagttcga tcatggtggt aatgtcgtgc aggtgcggca ggttgcagac ggtgaccgga gtatcggcca gcagggtcgc ggccaggatc ggcagcgccg agttcttcgc gccggaaatg cggatttcgc catcgaggcg gttaccgccg gtaataatca gtttatcc
Expressed Protein Sequence
MAHHHHHHMD KLIITGGNRL DGEIRISGAK NSALPILAAT LLADTPVTVC NLPHLHDITT MIELFGRMGV QPIIDEKLNV EVDASSIKTL VAPYELVKTM RASILVLGPM LARFGEAEVA LPGGCAIGSR PVDLHIRGLE AMGAQIEVEG GYIKAKAPAG GLRGGHFFFD TVSVTGTENL MMAAALANGR TVLQNAAREP EVVDLANCLN AMGANVQGAG SDTIVIEGVK RLGGARYDVL PDRIETGTYL VAAAATGGRV KLKDTDPTIL EAVLQKLEEA GAHISTGSNW IELDMKGNRP KAVNVRTAPY PAFPTDMQAQ FISMNAVAEG TGAVIETVFE NRFMHVYEMN RMGAQILVEG NTAIVTGVPK LKGAPVMATD LRASASLVIA GLVAEGDTLI DRIYHIDRGY ECIEEKLQLL GAKIRRVPG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATA AACTGATTAT TACCGGCGGT AACCGCCTCG ATGGCGAAAT CCGCATTTCC GGCGCGAAGA ACTCGGCGCT GCCGATCCTG GCCGCGACCC TGCTGGCCGA TACTCCGGTC ACCGTCTGCA ACCTGCCGCA CCTGCACGAC ATTACCACCA TGATCGAACT GTTCGGCCGC ATGGGCGTGC AGCCGATCAT CGACGAGAAG CTCAACGTCG AAGTCGATGC CAGCAGCATC AAAACCCTGG TCGCGCCGTA CGAACTGGTG AAGACCATGC GTGCCTCGAT CCTGGTGCTG GGCCCGATGC TGGCGCGCTT CGGCGAGGCC GAAGTGGCCC TGCCGGGCGG TTGCGCGATC GGTTCGCGTC CGGTCGACCT GCATATCCGC GGTCTCGAGG CCATGGGCGC GCAGATCGAG GTCGAAGGCG GCTACATCAA GGCCAAGGCG CCGGCCGGCG GCCTGCGTGG CGGTCACTTC TTCTTCGATA CCGTCAGCGT GACCGGCACC GAGAACCTGA TGATGGCCGC CGCGCTGGCC AACGGCCGTA CCGTGCTGCA GAACGCCGCT CGCGAGCCGG AGGTGGTCGA CCTGGCCAAC TGCCTGAACG CCATGGGCGC CAACGTCCAG GGCGCTGGCT CCGATACCAT CGTCATCGAA GGCGTGAAGC GCCTCGGCGG TGCTCGCTAC GACGTACTGC CCGACCGTAT CGAGACCGGC ACCTACCTGG TGGCCGCGGC CGCGACCGGT GGCCGGGTGA AGCTGAAGGA TACCGACCCG ACCATCCTCG AGGCGGTCCT GCAGAAGCTG GAAGAGGCCG GTGCCCACAT CAGCACCGGC AGCAACTGGA TCGAGCTGGA CATGAAGGGC AACCGGCCGA AGGCGGTCAA CGTGCGTACC GCGCCGTACC CGGCGTTCCC CACCGACATG CAGGCCCAGT TCATCTCCAT GAACGCGGTA GCCGAAGGCA CCGGCGCGGT CATCGAGACG GTCTTCGAGA ACCGCTTCAT GCATGTTTAC GAAATGAACC GCATGGGCGC GCAAATCCTC GTCGAAGGCA ACACCGCCAT CGTCACCGGC GTACCCAAGC TCAAGGGCGC TCCGGTCATG GCGACCGACC TGCGCGCATC CGCGAGCCTG GTGATCGCCG GCCTGGTGGC CGAAGGCGAC ACCCTGATCG ATCGCATCTA CCACATCGAC CGTGGCTACG AGTGCATCGA AGAGAAACTC CAGCTGCTCG GCGCCAAGAT CCGCCGCGTA CCGGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T