PsaeA.00944.a
Ribose-5-phosphate isomerase A (EC 5.3.1.6) (Phosphoriboisomerase A) (PRI)
| CENTER ID: | PsaeA.00944.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00944.a.B1.GE38257 | full length | 1 | 223 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00944.a.B1.PS02170 | full length | 1 | 223 |
Structures
4X84
DEPOSITED: 12/10/2014
DETERMINATION: XRay
CLONE: PsaeA.00944.a.B1.GE38257
PROTEIN: PsaeA.00944.a.B1.PS02170
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.346 |
| PGAT: | pseudomonas:PA0330 |
| RefSeq: | NP_249021.1 |
| UniProt: | Q9I6G1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQDQLKQAV AQAAVDHILP HLDSKSIVGV GTGSTANFFI DALARHKAEF DGAVASSEAT AKRLKEHGIP VYELNTVSEL EFYVDGADES NERLELIKGG GAALTREKIV AAVAKTFICI ADASKLVPIL GQFPLPVEVI PMARSHVARQ LVKLGGDPVY REGVLTDNGN IILDVHNLRI DSPVELEEKI NAIVGVVTNG LFAARPADLL LLGTADGVKT LKA
NT Sequence
atgaaccagg atcagctcaa gcaggcagtg gcacaggccg ccgtcgacca cattcttccg cacctcgaca gcaagagcat cgtcggcgtc ggcacaggct ccaccgccaa cttcttcatc gatgccctgg cccgccacaa ggccgagttc gacggcgcag tagccagttc ggaggccacc gccaagcgcc tgaaggagca tggcatcccg gtctacgagc tgaacacggt cagcgagctg gagttctacg tcgacggcgc cgacgaaagc aacgagcgcc tggagctgat caagggcggc ggtgccgcgc tgacccgcga gaagatcgtc gccgcggtgg ccaagacctt catctgcatc gccgatgcca gcaagctggt gcccatcctc ggccagttcc cgctgccggt ggaggtcatc ccgatggctc gcagccacgt cgcccgccaa ctggtgaagc tcggcggcga tccggtctac cgcgaaggcg tgctgaccga caacggcaat atcatcctcg acgtgcacaa cctgcgcatc gacagcccgg tggaactgga agagaagatc aacgccatcg tcggcgtggt caccaacggc ttgttcgccg cccgcccggc cgacctgctg ctactcggca ccgccgacgg cgtcaagacc ctcaaggcct ga
Details for PsaeA.00944.a.B1.GE38257
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMN QDQLKQAVAQ AAVDHILPHL DSKSIVGVGT GSTANFFIDA LARHKAEFDG AVASSEATAK RLKEHGIPVY ELNTVSELEF YVDGADESNE RLELIKGGGA ALTREKIVAA VAKTFICIAD ASKLVPILGQ FPLPVEVIPM ARSHVARQLV KLGGDPVYRE GVLTDNGNII LDVHNLRIDS PVELEEKINA IVGVVTNGLF AARPADLLLL GTADGVKTLK A
Validated NT Sequence
tgttagcagc cggatcctat cttactcact taggccttga gggtcttgac gccgtcggcg gtgccgagta gcagcaggtc ggccgggcgg gcggcgaaca agccgttggt gaccacgccg acgatggcgt tgatcttctc ttccagttcc accgggctgt cgatgcgcag gttgtgcacg tcgaggatga tattgccgtt gtcggtcagc acgccttcgc ggtagaccgg atcgccgccg agcttcacca gttggcgggc gacgtggctg cgagccatcg ggatgacctc caccggcagc gggaactggc cgaggatggg caccagcttg ctggcatcgg cgatgcagat gaaggtcttg gccaccgcgg cgacgatctt ctcgcgggtc agcgcggcac cgccgccctt gatcagctcc aggcgctcgt tgctttcgtc ggcgccgtcg acgtagaact ccagctcgct gaccgtgttc agctcgtaga ccgggatgcc atgctccttc aggcgcttgg cggtggcctc cgaactggct actgcgccgt cgaactcggc cttgtggcgg gccagggcat cgatgaagaa gttggcggtg gagcctgtgc cgacgccgac gatgctcttg ctgtcgaggt gcggaagaat gtggtcgacg gcggcctgtg ccactgcctg cttgagctga tcctggttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN QDQLKQAVAQ AAVDHILPHL DSKSIVGVGT GSTANFFIDA LARHKAEFDG AVASSEATAK RLKEHGIPVY ELNTVSELEF YVDGADESNE RLELIKGGGA ALTREKIVAA VAKTFICIAD ASKLVPILGQ FPLPVEVIPM ARSHVARQLV KLGGDPVYRE GVLTDNGNII LDVHNLRIDS PVELEEKINA IVGVVTNGLF AARPADLLLL GTADGVKTLK A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc aggatcagct caagcaggca gtggcacagg ccgccgtcga ccacattctt ccgcacctcg acagcaagag catcgtcggc gtcggcacag gctccaccgc caacttcttc atcgatgccc tggcccgcca caaggccgag ttcgacggcg cagtagccag ttcggaggcc accgccaagc gcctgaagga gcatggcatc ccggtctacg agctgaacac ggtcagcgag ctggagttct acgtcgacgg cgccgacgaa agcaacgagc gcctggagct gatcaagggc ggcggtgccg cgctgacccg cgagaagatc gtcgccgcgg tggccaagac cttcatctgc atcgccgatg ccagcaagct ggtgcccatc ctcggccagt tcccgctgcc ggtggaggtc atcccgatgg ctcgcagcca cgtcgcccgc caactggtga agctcggcgg cgatccggtc taccgcgaag gcgtgctgac cgacaacggc aatatcatcc tcgacgtgca caacctgcgc atcgacagcc cggtggaact ggaagagaag atcaacgcca tcgtcggcgt ggtcaccaac ggcttgttcg ccgcccgccc ggccgacctg ctgctactcg gcaccgccga cggcgtcaag accctcaagg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PsaeA.00944.a.B1.PS02170
| PURIFICATION DATe: | 9/17/2014 |
| CONCENTRATION: | 29.94mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN QDQLKQAVAQ AAVDHILPHL DSKSIVGVGT GSTANFFIDA LARHKAEFDG AVASSEATAK RLKEHGIPVY ELNTVSELEF YVDGADESNE RLELIKGGGA ALTREKIVAA VAKTFICIAD ASKLVPILGQ FPLPVEVIPM ARSHVARQLV KLGGDPVYRE GVLTDNGNII LDVHNLRIDS PVELEEKINA IVGVVTNGLF AARPADLLLL GTADGVKTLK A
Validated NT Sequence
tgttagcagc cggatcctat cttactcact taggccttga gggtcttgac gccgtcggcg gtgccgagta gcagcaggtc ggccgggcgg gcggcgaaca agccgttggt gaccacgccg acgatggcgt tgatcttctc ttccagttcc accgggctgt cgatgcgcag gttgtgcacg tcgaggatga tattgccgtt gtcggtcagc acgccttcgc ggtagaccgg atcgccgccg agcttcacca gttggcgggc gacgtggctg cgagccatcg ggatgacctc caccggcagc gggaactggc cgaggatggg caccagcttg ctggcatcgg cgatgcagat gaaggtcttg gccaccgcgg cgacgatctt ctcgcgggtc agcgcggcac cgccgccctt gatcagctcc aggcgctcgt tgctttcgtc ggcgccgtcg acgtagaact ccagctcgct gaccgtgttc agctcgtaga ccgggatgcc atgctccttc aggcgcttgg cggtggcctc cgaactggct actgcgccgt cgaactcggc cttgtggcgg gccagggcat cgatgaagaa gttggcggtg gagcctgtgc cgacgccgac gatgctcttg ctgtcgaggt gcggaagaat gtggtcgacg gcggcctgtg ccactgcctg cttgagctga tcctggttca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMN QDQLKQAVAQ AAVDHILPHL DSKSIVGVGT GSTANFFIDA LARHKAEFDG AVASSEATAK RLKEHGIPVY ELNTVSELEF YVDGADESNE RLELIKGGGA ALTREKIVAA VAKTFICIAD ASKLVPILGQ FPLPVEVIPM ARSHVARQLV KLGGDPVYRE GVLTDNGNII LDVHNLRIDS PVELEEKINA IVGVVTNGLF AARPADLLLL GTADGVKTLK A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC AGGATCAGCT CAAGCAGGCA GTGGCACAGG CCGCCGTCGA CCACATTCTT CCGCACCTCG ACAGCAAGAG CATCGTCGGC GTCGGCACAG GCTCCACCGC CAACTTCTTC ATCGATGCCC TGGCCCGCCA CAAGGCCGAG TTCGACGGCG CAGTAGCCAG TTCGGAGGCC ACCGCCAAGC GCCTGAAGGA GCATGGCATC CCGGTCTACG AGCTGAACAC GGTCAGCGAG CTGGAGTTCT ACGTCGACGG CGCCGACGAA AGCAACGAGC GCCTGGAGCT GATCAAGGGC GGCGGTGCCG CGCTGACCCG CGAGAAGATC GTCGCCGCGG TGGCCAAGAC CTTCATCTGC ATCGCCGATG CCAGCAAGCT GGTGCCCATC CTCGGCCAGT TCCCGCTGCC GGTGGAGGTC ATCCCGATGG CTCGCAGCCA CGTCGCCCGC CAACTGGTGA AGCTCGGCGG CGATCCGGTC TACCGCGAAG GCGTGCTGAC CGACAACGGC AATATCATCC TCGACGTGCA CAACCTGCGC ATCGACAGCC CGGTGGAACT GGAAGAGAAG ATCAACGCCA TCGTCGGCGT GGTCACCAAC GGCTTGTTCG CCGCCCGCCC GGCCGACCTG CTGCTACTCG GCACCGCCGA CGGCGTCAAG ACCCTCAAGG CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT