PsaeA.00941.b

Uncharacterized protein (HHPRED: putative dna replication regulator)

CENTER ID: PsaeA.00941.b
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00941.b.B1.GE38256 full length 1 234
PsaeA.00941.b.B2.GE39839 N-term truncated 15 233
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.984
PGAT: pseudomonas:PA0947
RefSeq: NP_249638.1
UniProt: Q9I511

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPIQLPLSV RLRDDATFAN YYPGANAAAL GYVERLCEAE AGWAESLIYL WGHDGVGRSH LLQAACLRFE QFEERTIYLP MADLVQYGPE IFDDLEQCEL VCIDDLDVLV GKREWEEGLF HLFNRLRDTG RRLLLAASKS PRELQVKLPD LKSRLTMALI FQLHGLSDED KLRALQLRAS RRGLHLTDEV GRFILNRGSR SMNSLFDLLE QLDRASLQAQ RKLTIPFLKE TLGW
NT Sequence
atgaaaccta ttcagcttcc cctcagtgtg cgtctgcgcg acgacgccac cttcgccaac tactatcccg gtgccaatgc cgcggcactg ggatatgtcg agcgtttgtg cgaagccgag gctggctggg cggaaagcct catctacctc tggggacacg acggcgtcgg ccgcagccac ctgttgcagg cggcctgctt gcgtttcgag cagttcgaag agcgaaccat ctacctgccg atggccgatc tggtgcagta cggcccggaa atcttcgacg atctcgagca gtgcgagctg gtctgcatcg acgacctgga tgtgctggtt ggcaagcgag aatgggaaga ggggctgttc cacctgttca atcgactgcg tgataccggt cggcgcctgc tgctggcggc atcgaagtcg ccccgggagc tgcaggtcaa gctgccggac ctgaagtccc gcctcaccat ggcgctgatc ttccagttgc acggtctttc cgacgaggac aagcttcgtg ccctgcaact gcgggcatcc cgtcgtggcc tgcatctgac cgatgaagtc ggtcgtttca tcctcaaccg cggttcgcgc agcatgaact ctctgttcga tctgctggag caactggatc gcgcttcgct ccaggctcag cgcaagctga ccattccttt cctcaaggaa accctcggct ggtaa
Details for PsaeA.00941.b.B1.GE38256
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK PIQLPLSVRL RDDATFANYY PGANAAALGY VERLCEAEAG WAESLIYLWG HDGVGRSHLL QAACLRFEQF EERTIYLPMA DLVQYGPEIF DDLEQCELVC IDDLDVLVGK REWEEGLFHL FNRLRDTGRR LLLAASKSPR ELQVKLPDLK SRLTMALIFQ LHGLSDEDKL RALQLRASRR GLHLTDEVGR FILNRGSRSM NSLFDLLEQL DRASLQAQRK LTIPFLKETL GW
Validated NT Sequence
tgtagcagcc ggatcctatc ttactcactt accagccgag ggtttccttg aggaaaggaa tggtcagctt gcgctgagcc tggagcgaag cgcgatccag ttgctccagc agatcgaaca gagagttcat gctgcgcgaa ccgcggttga ggatgaaacg accgacttca tcggtcagat gcaggccacg acgggatgcc cgcagttgca gggcacgaag cttgtcctcg tcggaaagac cgtgcaactg gaagatcagc gccatggtga ggcgggactt caggtccggc agcttgacct gcagctcccg gggcgacttc gatgccgcca gcagcaggcg ccgaccggta tcacgcagtc gattgaacag gtggaacagc ccctcttccc attctcgctt gccaaccagc acatccaggt cgtcgatgca gaccagctcg cactgctcga gatcgtcgaa gatttccggg ccgtactgca ccagatcggc catcggcagg tagatggttc gctcttcgaa ctgctcgaaa cgcaagcagg ccgcctgcaa caggtggctg cggccgacgc cgtcgtgtcc ccagaggtag atgaggcttt ccgcccagcc agcctcggct tcgcacaaac gctcgacata tcccagtgcc gcggcattgg caccgggata gtagttggcg aaggtggcgt cgtcgcgcag acgcacactg aggggaagct gaataggttt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK PIQLPLSVRL RDDATFANYY PGANAAALGY VERLCEAEAG WAESLIYLWG HDGVGRSHLL QAACLRFEQF EERTIYLPMA DLVQYGPEIF DDLEQCELVC IDDLDVLVGK REWEEGLFHL FNRLRDTGRR LLLAASKSPR ELQVKLPDLK SRLTMALIFQ LHGLSDEDKL RALQLRASRR GLHLTDEVGR FILNRGSRSM NSLFDLLEQL DRASLQAQRK LTIPFLKETL GW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac ctattcagct tcccctcagt gtgcgtctgc gcgacgacgc caccttcgcc aactactatc ccggtgccaa tgccgcggca ctgggatatg tcgagcgttt gtgcgaagcc gaggctggct gggcggaaag cctcatctac ctctggggac acgacggcgt cggccgcagc cacctgttgc aggcggcctg cttgcgtttc gagcagttcg aagagcgaac catctacctg ccgatggccg atctggtgca gtacggcccg gaaatcttcg acgatctcga gcagtgcgag ctggtctgca tcgacgacct ggatgtgctg gttggcaagc gagaatggga agaggggctg ttccacctgt tcaatcgact gcgtgatacc ggtcggcgcc tgctgctggc ggcatcgaag tcgccccggg agctgcaggt caagctgccg gacctgaagt cccgcctcac catggcgctg atcttccagt tgcacggtct ttccgacgag gacaagcttc gtgccctgca actgcgggca tcccgtcgtg gcctgcatct gaccgatgaa gtcggtcgtt tcatcctcaa ccgcggttcg cgcagcatga actctctgtt cgatctgctg gagcaactgg atcgcgcttc gctccaggct cagcgcaagc tgaccattcc tttcctcaag gaaaccctcg gctggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.00941.b.B2.GE39839
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD ATFANYYPGA NAAALGYVER LCEAEAGWAE SLIYLWGHDG VGRSHLLQAA CLRFEQFEER TIYLPMADLV QYGPEIFDDL EQCELVCIDD LDVLVGKREW EEGLFHLFNR LRDTGRRLLL AASKSPRELQ VKLPDLKSRL TMALIFQLHG LSDEDKLRAL QLRASRRGLH LTDEVGRFIL NRGSRSMNSL FDLLEQLDRA SLQAQRKLTI PFLKETLG
Validated NT Sequence
atcttactca cttagccgag ggtttccttg aggaaaggaa tggtcagctt gcgctgagcc tggagcgaag cgcgatccag ttgctccagc agatcgaaca gagagttcat gctgcgcgaa ccgcggttga ggatgaaacg accgacttca tcggtcagat gcaggccacg acgggatgcc cgcagttgca gggcacgaag cttgtcctcg tcggaaagac cgtgcaactg gaagatcagc gccatggtga ggcgggactt caggtccggc agcttgacct gcagctcccg gggcgacttc gatgccgcca gcagcaggcg ccgaccggta tcacgcagtc gattgaacag gtggaacagc ccctcttccc attctcgctt gccaaccagc acatccaggt cgtcgatgca gaccagctcg cactgctcga gatcgtcgaa gatttccggg ccgtactgca ccagatcggc catcggcagg tagatggttc gctcttcgaa ctgctcgaaa cgcaagcagg ccgcctgcaa caggtggctg cggccgacgc cgtcgtgtcc ccagaggtag atgaggcttt ccgcccagcc agcctcggct tcgcacaaac gctcgacata tcccagtgcc gcggcattgg caccgggata gtagttggcg aaggtggcgt ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHDA TFANYYPGAN AAALGYVERL CEAEAGWAES LIYLWGHDGV GRSHLLQAAC LRFEQFEERT IYLPMADLVQ YGPEIFDDLE QCELVCIDDL DVLVGKREWE EGLFHLFNRL RDTGRRLLLA ASKSPRELQV KLPDLKSRLT MALIFQLHGL SDEDKLRALQ LRASRRGLHL TDEVGRFILN RGSRSMNSLF DLLEQLDRAS LQAQRKLTIP FLKETLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgcca ccttcgccaa ctactatccc ggtgccaatg ccgcggcact gggatatgtc gagcgtttgt gcgaagccga ggctggctgg gcggaaagcc tcatctacct ctggggacac gacggcgtcg gccgcagcca cctgttgcag gcggcctgct tgcgtttcga gcagttcgaa gagcgaacca tctacctgcc gatggccgat ctggtgcagt acggcccgga aatcttcgac gatctcgagc agtgcgagct ggtctgcatc gacgacctgg atgtgctggt tggcaagcga gaatgggaag aggggctgtt ccacctgttc aatcgactgc gtgataccgg tcggcgcctg ctgctggcgg catcgaagtc gccccgggag ctgcaggtca agctgccgga cctgaagtcc cgcctcacca tggcgctgat cttccagttg cacggtcttt ccgacgagga caagcttcgt gccctgcaac tgcgggcatc ccgtcgtggc ctgcatctga ccgatgaagt cggtcgtttc atcctcaacc gcggttcgcg cagcatgaac tctctgttcg atctgctgga gcaactggat cgcgcttcgc tccaggctca gcgcaagctg accattcctt tcctcaagga aaccctcggc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat